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Wu B.,Jilin University | Wu B.,Changchun Institute of Technology | Cong Q.,Jilin University | Sun T.,Changchun Keygen Biological Products Co. | And 2 more authors.
Harbin Gongcheng Daxue Xuebao/Journal of Harbin Engineering University | Year: 2016

Considering the huge numbers of internal combustion engines in use worldwide, even a small reduction in the friction of the piston-cylinder sleeve system would greatly impact energy conservation and emission reduction. In this paper, we use the LX-2V engine as a test matrix. First, we applied drag reduction and a wear-resistant stripe pattern on a shell's surface to the main friction pair of the internal combustion engine-piston skirt. We then constructed a three-level and three-factor orthogonal test plan. Under the worst skirt conditions, we analyzed the finite element contact for the standard piston and nine bionic piston models, and obtained and compared the three most typical test indexes. We then used a range analysis method to test and optimize the design. Lastly, we selected a standard piston, a bionic piston with optimum performance, and a piston with an optimum combination on which to carry out a durability bench test of the internal combustion engine. Results show that the bionic piston performs better than the standard piston regarding the unloading of concentrated stress of the piston's oil return hole, drag reduction, and abrasion resistance. The average abrasion loss of the bionic piston was 42.9% less than that of the standard piston. In addition, the average cylinder pressure changing rate of the bionic piston was 50.2% more stable than that of the standard piston. © 2016, Editorial Board of Journal of Harbin Engineering. All right reserved. Source


Liang H.-Y.,Changchun Keygen Biological Products Co. | Zou Y.,Changchun Keygen Biological Products Co.
Chinese Journal of New Drugs | Year: 2012

Varicella, a common infectious disease in childhood, is caused by varicella-zoster virus. The initial infection is manifested as varicella, and the virus continues to lurk in the spinal ganglia. When the body is in a pathological condition or the immune function is decreased, it produces shingles. Freeze dried live attenuated varicella vaccine is prepared by varicella-zoster virus strain, and it is the only means of varicella infection prevention. Here, we reviewed the development status of freeze dried live attenuated varicella vaccine at home and abroad and the development trend in the future. Source


Zhang Y.,Changchun Keygen Biological Products Co. | Hao B.-S.,Changchun Keygen Biological Products Co. | Zhao H.-B.,Changchun Keygen Biological Products Co.
Chinese Journal of New Drugs | Year: 2012

Original infection of varicella-zoster virus can induce varicella and then herpes when the virus is activated again. In recent years, with the progress of aging of domestic population, the incidence rate of herpes is increasing apparently, and there is no apparent therapeutic effect to treat post-herpetic neuralgia caused by herpes with antiviral drugs. Therefore, the prevention and therapy of herpes has been attached more and more importance for it has become an important public hygiene issue. The prevention of herpes with vaccine has been accepted by the society widely. In this article, we summarized the causative agent of herpes and the current situation of research and development of vaccine research. Source


Liang X.,Changchun Keygen Biological Products Co. | Liu Y.-W.,Changchun Keygen Biological Products Co. | Zhang Y.,Changchun Keygen Biological Products Co. | Shi L.,Changchun Keygen Biological Products Co. | And 3 more authors.
Chinese Journal of Biologicals | Year: 2014

Objective To develop a culture procedure for 2BS cell factory during production of herpes zoster vaccine. Methods The 2BS cells were resuscitated (passage 28) and subcultured to passages 35 and 36, then to the ten layer cell factory at mass ratios of 1 : 4 and 1 : 2 respectively. The cells on each layer were added with various volumes (200, 300 and 400 ml) of medium, observed for morphology by microscopy and counted. The cell factory was inoculated with varicella- zoster virus at a MOI of 0. 008 and prepared into live attenuated vaccine. The bulk and final product of vaccine were determined for titers. The final product was subjected to stability test at 37 °C. The residual antibiotics and bovine albumin contents were determined by ELISA, while the endotoxin content by gel method, and the moisture content by volume titration method. Results Dense 2BS cell monolayer with high quality was prepared by subculture at a mass ratio of 1 : 2 and addition of 300 ml of medium, of which the CPE reached more than 80% at time of harvest. The cells with CPE were round, most of which were in typical shape. The titers of bulk and final product of vaccine prepared with cells subcultured at a mass ratio of 1 : 4 were significantly lower than those at 1 : 2. However, the titer in stability test at 37 °C. of final product of vaccine prepared with the cells subcultured at a mass ratio of 1 : 4 failed to met the requirements of not less than 4. 6 lgPFU/ml after storage for one week. Other indexes met the relevant requirements. Conclusion A culture procedure for 2BS cell factory during production of herpes zoster vaccine was developed, by which dense 2BS cells with even and high quality were prepared. Source


Dai C.-H.,Changchun Keygen Biological Products Co. | Wang F.,Changchun Keygen Biological Products Co. | Shan X.-F.,Changchun Keygen Biological Products Co. | Li Q.,Changchun Keygen Biological Products Co. | And 3 more authors.
Chinese Journal of Biologicals | Year: 2013

Objective: To compare the culture effects of human diploid cell 2BS strain with disposable culture flask and with common flask. Methods The 2BS cells at the same concentrations were inoculated into disposable culture flask and small common flask respectively, and incubated at (37 ± 0. 5) °C for 3 d, observed for growth under microscope and counted, then infected with varicella-zoster virus (VZV) Oka strain at a MOI of 0.025-0.05 and incubated at (35 ± 0.5) °C for 3 d. The cells were harvested when CPE reached more than 70% to prepare freeze-dried live attenuated varicella vaccine. Overall control tests were performed on the prepared vaccine according to the standard for registration of live attenuated varicella vaccine and the requirements in Chinese Pharmacopoeia(Volume III, 2010 edition). Results: At the same concentrations for inoculation, the area of 2BS cell culture in disposable culture flask increased as compared with that in common flask, while the total cell count was relatively large. The 2BS cells in disposable flask became adherent rapidly, which were in spindle shape, at high density, with good directivity, clear outline and full cytoplasm, and in even distribution. However, the 2BS cells in common flask became adherent slowly and were uneven in growth. All the quality indexes of vaccines prepared with 2BS cells cultured in both disposable culture flask and common flask met the requirements in Chinese Pharmacopoeia (Volume III, 2010 edition). If the virus titers showed no significant difference, the yield of varicella vaccine prepared with 2BS cells cultured in disposable culture flask increased remarkably. Conclusion: The yield of live attenuated varicella vaccine prepared with 2BS cells cultured in disposable culture flask increased remarkably as compared with that in common flask. Source

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