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Elphine Prabahar A.,Chalapathi Institute of Pharmaceutical Science | Vijayaraj Kumar P.,Bharat Institute of Pharmacy
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2010

Two simple, rapid and sensitive extractive spectrophotometry methods have been developed for the assay of cinitapride in pure and pharmaceutical formulations. These methods are based on the formation of chloroform soluble ion-association complexes of cinitapride with Bromocresol Green (BCG) and with Bromothymol Blue (BTB) in potassium hydrogen phthalate buffer pH-4 with absorption maximum at 414 nm and 416 nm for BCG and BTB, respectively. Reaction conditions were optimized to obtain the maximum colour intensity. The absorbance was found to increase linearly with increase in concentration of cinitapride, which was corroborated by the calculated correlation coefficient values (0.9999 and 0.9998). The systems obeyed Beer's law in the range of 5-40 and 2-10 μg/mL for BCG and BTB, respectively. Various analytical parameters have been evaluated and the results have been validated by statistical data. No interference was observed from common excipients present in tablets.


Arifa Begum S.K.,Chalapathi Institute of Pharmaceutical science | Rama Rao N.,Chalapathi Institute of Pharmaceutical science
Der Pharmacia Lettre | Year: 2013

A simple, specific, accurate, precise and reproducible method has been developed and validated for the simultaneous estimation of Rifampicin and Isoniazid in combined dosage form by UV Spectrophotometric method. UV spectrophotometric method includes simultaneous equation method using 337.0 nm and 263.0 nm λmax of both the drugs were selected for estimation of Rifampicin and Isoniazid respectively. Rifampicin and Isoniazid follow Beer's law over the concentration range of 5-35 μg/ml and 5-25 μg/ml. The percentage recoveries of the both the drugs were found to be nearly 100 % representing the accuracy of the proposed methods. Validation of the proposed methods was carried out for its accuracy, precision, and specificity according to ICH guidelines. The proposed method can be successfully applied in routine laboratory analysis for the determination of Rifampicin and Isoniazid in combined dosage form.


Sathish Kumar M.,Chalapathi Institute of Pharmaceutical science | Nadendla R.,Chalapathi Institute of Pharmaceutical science
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014

The study was undertaken to evaluate the analgesic and anti-inflammatory activity of Tragia plukenetii R. Smith leaf extracts using eddy's hot plate method, undiluted fresh egg albumin induced rat paw oedema method respectively. The study comprised of four treatment groups (control, standard and test - Tragia plukenetii aqueous and methanolic leaf extract) all with six animals in each group. At the end of the study aqueous leaf extract showed significant analgesic activity when compared with methanolic leaf extract, standard and control treatment groups.


Sathish Kumar M.,Chalapathi Institute of Pharmaceutical science | Farzana S.K.,Chalapathi Institute of Pharmaceutical science | Ramarao N.,Chalapathi Institute of Pharmaceutical science
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014

The study was undertaken to evaluate the anti-catatonic effects of Tragia plukenetii R. Smith methanolic leaf extracts using phenothiazine induced catatonia in rats. Catatonia was produced by the administration of phenothiazine in Wistar rats. The study comprised of three treatment groups (Control, Standard and Test - Tragia plukenetii methanolic leaf extract) all with five animals in each group. Severity of catatonia was observed and scored. At the end of the study the test treated (TPME) group showed significant anticatatonic effect, showing an overall p value <0.0001 when compared with other treatment groups i.e., control and standard.


Rama Rao N.,Chalapathi Institute of Pharmaceutical science
E-Journal of Chemistry | Year: 2010

Simple, sensitive, accurate, precise and rapid ultraviolet (UV) spectrophotometric method was developed for the estimation of efavirenz in pure form, its formulations and stability samples. For the estimation of efavirenz, solvent system employed was 1% w/v sodium lauryl sulphate (SLS) and wavelength of detection (λdet) was 247 nm. The developed method was used to estimate the total drug content in two commercially available oral formulations of efavirenz and recovery studies were also carried out. Sample recovery in both the formulations using the above method was in good agreement with their respective labeled claims, thus suggesting the validity of the method and non-interference of formulation excipients in the estimation. The developed method was found to be stability specific and were validated as per ICH guidelines-2005, USP-2000 and statistical methods.


Kumar M.S.,Chalapathi Institute of Pharmaceutical science | Tony D.E.,Chalapathi Institute of Pharmaceutical science | Rao N.R.,Chalapathi Institute of Pharmaceutical science
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013

Hebbs William maze is used for studying learning, memory and reasoning in animals. The clever the rat, the more quickly it is able to make use of past experience and therefore more quickly it learns its way out in the maze. In the present study, we evaluated the effects of Tragia plukenetii. ith leaf extracts on learning, memory and reasoning model using the Hebbs William maze in rats.


Ponnuru V.S.,Chalapathi Institute of Pharmaceutical science | Ponnuru V.S.,Andhra University | Nadendla R.,Chalapathi Institute of Pharmaceutical science
Journal of Pharmaceutical Analysis | Year: 2012

Obesity can be considered as a chronic illness of epidemic proportion and its incidents have increased exponentially in recent years. The use of anti-obesity drugs such as sibutramine is somewhat helpful. There is a need to quantify such drugs in biological samples, which is generally quite difficult. In this report, we developed and validated a simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of sibutramine (SB) and its two metabolites N-des methyl sibutramine (DSB) and N-di desmethyl sibutramine (DDSB) in human plasma. Zorbax SB-C18 (4.6 mm×75 mm, 3.5 μm, 80 Å) analytical column and 5 mM ammonium formate:acetonitrile (10:90, v/v) mobile phase were used for chromatographic separation of SB, DSB and DDSB. Multiple reaction monitoring (MRM) in the positive mode was used to detect SB, DSB and DDSB at m/z 280.3/124.9, 266.3/125.3 and 252.2/124.9, respectively. Liquid-liquid extraction was used for the extraction of analytes and internal standard from human plasma. This method was validated over a linear concentration range of 10.0-10,000.0 pg/mL for SB, DSB and DDSB with correlation coefficients (r) of ≥0.9997. The drug and the two metabolites were stable in plasma samples. The validated method was successfully applied in a bioequivalence and pharmacokinetic study with human volunteers under fasting condition. © 2012 Xi'an Jiaotong University.


Ponnuru V.S.,Chalapathi Institute of Pharmaceutical science | Ponnuru V.S.,Krishna University | Nadendla R.,Chalapathi Institute of Pharmaceutical science
Journal of Pharmaceutical Analysis | Year: 2012

A simple, sensitive, and specific liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of desloratadine (DL) in human plasma using desloratadine-d5 (DLD5) as an internal standard (IS). Chromatographic separation was performed using an Xbridge C18 column (50 mm×4.6 mm, 5 μm) with an isocratic mobile phase composed of 10 mM ammonium formate: methanol (20:80, v/v), at a flow rate of 0.7 mL/min. DL and DLD5 were detected with proton adducts at m/z 311.2→259.2 and 316.2→264.3 in multiple reaction monitoring (MRM) positive modes, respectively. Liquid-liquid extraction (LLE) method was used to extract the drug and the IS. The method was validated over a linear concentration range of 5.0-5000.0 pg/mL with a correlation coefficient of (r2)≥0.9994. This method demonstrated intra- and inter-day precision within 0.7-2.0% and 0.7-2.7%, and an accuracy within 101.4-102.4%, and 99.5-104.8%. DL was found to be stable throughout the freeze-thaw cycles, bench-top, and postoperative stability studies. This method was successfully applied in the analysis of plasma samples following oral administration of DL (5 mg) in 35 healthy Indian male human volunteers under fasting conditions. © 2012 Xian Jiaotong University. Production and hosting by Elsevier B.V. All rights reserved.


Lakshmi K.,Chalapathi Institute of Pharmaceutical science | Rama Rao N.,Chalapathi Institute of Pharmaceutical science | Basaveswararao M.V.,Krishna University
Rasayan Journal of Chemistry | Year: 2014

The objective of the present study was synthesis, physical and spectral characterization, in vitro antimicrobial and anthelmintic evaluation of a series of some novel quinazolinonyl chalcones as better leads. The titled compounds (3a-r) with IUPAC name {3-phenyl-1-[3'-(un) substituted phenyl-6'-(un) substituted-2'-thioxo-4'(3'H)-quinazolinon- 1'-yl]-2-propen-1-ones} have been synthesized by Claisen-Schmidt condensation reaction between substituted 1- acetyl-2-thioxoquinazolin-4(3H)-ones (2a-r) and aromatic aldehyde (Benzaldehyde). All the synthesized compounds have been confirmed by elemental, IR, 1HNMR and Mass spectral analyses. Further, they were evaluated for in vitro antibacterial, antifungal and anthelmintic activities against several pathogenic bacteria, fungi and helminth (Peritima posthuma) respectively. Out of 18 newly synthesized quinazolinonyl chalcones, titled compounds 3b, 3f, 3i, 3k, 3l and 3n showed excellent antimicrobial activity against all the selected pathogenic microorganisms. Significant anthelmintic activity was shown by titled compounds 3h, 3i, 3j, 3k, 3l, 3m and 3o in comparison with standard Albendazole. Finally, the titled compounds 3i, 3k and 3l showed significant activity in all the three in vitro evaluations and found to be the best among all the synthesized compounds in the series. © 2014 RASAYAN. All rights reserved.


Kotresha D.,Indian Institute of Science | Nedendla R.,Chalapathi Institute of Pharmaceutical Science
Indian Journal of Pharmacology | Year: 2011

Objective: This study was undertaken to evaluate the neuroprotective activity of Wedelia calendulacea against cerebral ischemia/reperfusion induced oxidative stress in the rats. Materials and Methods: The global cerebral ischemia was induced in male albino Wistar rats by occluding the bilateral carotid arteries for 30 min followed by 1 h and 4 h reperfusion. At various times of reperfusion, the histopathological changes and the levels of malondialdehyde (MDA), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione-s-transferase (GST), and hydrogen peroxide (H 2 O 2) activity and brain water content were measured. Results: The ischemic changes were preceded by increase in concentration of MDA, hydrogen peroxide and followed by decreased GPx, GR, and GST activity. Treatment with W. calendulacea significantly attenuated ischemia-induced oxidative stress. W. calendulacea administration markedly reversed and restored to near normal level in the groups pre-treated with methanolic extract (250 and 500 mg/kg, given orally in single and double dose/day for 10 days) in dose-dependent way. Similarly, W. calendulacea reversed the brain water content in the ischemia reperfusion animals. The neurodegenaration also conformed by the histopathological changes in the cerebral-ischemic animals. Conclusion: The findings from the present investigation reveal that W. calendulacea protects neurons from global cerebral-ischemic injury in rat by attenuating oxidative stress.

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