Chaitanya Postgraduate College

Kishanpura, India

Chaitanya Postgraduate College

Kishanpura, India
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PubMed | Krishna University, CSIR - Central Electrochemical Research Institute, Sri Krishnadevaraya University, Gandhi Institute of Technology and Management and 4 more.
Type: | Journal: Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis | Year: 2016

Mitochondrial displacement loop (D-loop) is the hot spot for mitochondrial DNA (mtDNA) alterations which influence the generation of cellular reactive oxygen species. In the present study, we sequenced the entire mitochondrial D-loop region (1124bp) of colorectal cancer (CRC) patients (n=174) and controls (n=170) of south Indian origin to identify significant mutations/polymorphisms. Our results showed 152 polymorphisms in the D-loop region of patients and/or controls. Polymorphisms were predominantly located in hypervariable region I (54.6%) than in II (45.4%) of D-loop region. The frequencies of 310C insertion (p=0.0078), T16189C (p=0.0097) variants and 310Cins/16189C haplotype (p=0.0029) were significantly higher in cases than in controls. Furthermore, strong linkage disequilibrium was observed between nucleotide position 310 and 16189 in cases (D=0.68) as compared with controls (D=0.27). In conclusion, mitochondrial D-loop sequence alterations may constitute inherent risk factor for CRC.


Tipirisetti N.R.,Osmania University | Govatati S.,Osmania University | Pullari P.,Periyar University | Malempati S.,Krishna University | And 8 more authors.
PLoS ONE | Year: 2014

Background: Mitochondrial displacement loop (D-loop) is the hot spot for mitochondrial DNA (mtDNA) alterations which influence the generation of cellular reactive oxygen species (ROS). Association of D-loop alterations with breast cancer has been reported in few ethnic groups; however none of the reports were documented from Indian subcontinent. Methodology: We screened the entire mitochondrial D-loop region (1124 bp) of breast cancer patients (n = 213) and controls (n = 207) of south Indian origin by PCR-sequencing analysis. Haplotype frequencies for significant loci, the standardized disequilibrium coefficient (D′) for pair-wise linkage disequilibrium (LD) were assessed by Haploview Software. Principal Findings: We identified 7 novel mutations and 170 reported polymorphisms in the D-loop region of patients and/or controls. Polymorphisms were predominantly located in hypervariable region I (60%) than in II (30%) of D-loop region. The frequencies of 310′C′ insertion (P = 0.018), T16189C (P = 0.0019) variants and 310′C′ins/16189C (P = 0.00019) haplotype were significantly higher in cases than in controls. Furthermore, strong LD was observed between nucleotide position 310 and 16189 in controls (D′ = 0.49) as compared to patients (D′ = 0.14). Conclusions: Mitochondrial D-loop alterations may constitute inherent risk factors for breast cancer development. The analysis of genetic alterations in the D-loop region might help to identify patients at high risk for bad progression, thereby helping to refine therapeutic decisions in breast cancer. © 2014 Tipirisetti et al.


PubMed | Krishna University, Nizam's Institute of Medical Sciences, Sri Krishnadevaraya University, Periyar University and 3 more.
Type: Journal Article | Journal: PloS one | Year: 2014

Mitochondrial displacement loop (D-loop) is the hot spot for mitochondrial DNA (mtDNA) alterations which influence the generation of cellular reactive oxygen species (ROS). Association of D-loop alterations with breast cancer has been reported in few ethnic groups; however none of the reports were documented from Indian subcontinent.We screened the entire mitochondrial D-loop region (1124 bp) of breast cancer patients (n=213) and controls (n=207) of south Indian origin by PCR-sequencing analysis. Haplotype frequencies for significant loci, the standardized disequilibrium coefficient (D) for pair-wise linkage disequilibrium (LD) were assessed by Haploview Software.We identified 7 novel mutations and 170 reported polymorphisms in the D-loop region of patients and/or controls. Polymorphisms were predominantly located in hypervariable region I (60%) than in II (30%) of D-loop region. The frequencies of 310C insertion (P=0.018), T16189C (P=0.0019) variants and 310Cins/16189C (P=0.00019) haplotype were significantly higher in cases than in controls. Furthermore, strong LD was observed between nucleotide position 310 and 16189 in controls (D=0.49) as compared to patients (D=0.14).Mitochondrial D-loop alterations may constitute inherent risk factors for breast cancer development. The analysis of genetic alterations in the D-loop region might help to identify patients at high risk for bad progression, thereby helping to refine therapeutic decisions in breast cancer.


Gullapelli K.,Kakatiya University | Thupurani M.K.,Chaitanya Postgraduate College | Brahmeshwari G.,Kakatiya University
International Journal of Pharma and Bio Sciences | Year: 2014

As a part of systematic investigation of synthesis, characterization and biological activities of N-(4-(1H-benzo(d)imidazole -2-yl)-2-(4-hydroxy -6-methyl pyramidine -2-yl thio) acetamide have been synthesized from 2-(4-aminophenyl) benzimidazole and 2-mercapto -4-hydroxy -6-methyl pyramidine, which have been prepared from p-amino benzoic acid and benzene 1,2 diamine and EAA with thiourea. All the synthesized compounds have been characterized by 1HNMR; IR spectral data. The prepared compounds were tested for antibacterial activity.


Murali Krishna T.,Chaitanya Postgraduate College | Vadluri R.,Chaitanya Postgraduate College | Manoj Kumar E.,Chaitanya Postgraduate College
International Journal of Pharma and Bio Sciences | Year: 2013

The present investigation was carried out to determine the antioxidant properties in methanolic extract of leaves, stem, fruit and roots of Physalis angulata (L). The antioxidant assay was carried out using free radicals DPPH, superoxide, nitric oxide, hydrogen peroxide and hydroxyl radical. Among the extracts tested fruit and leaf extracts were found to be significant in their antioxidant property than stem and roots. However, amounts of total phenolic and flavanoid content were high in leaves and fruit extracts.

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