Feher E.,Hungarian Academy of Sciences |
Szekely C.,Hungarian Academy of Sciences |
Lorincz M.,Szent Istvan University |
Cech G.,Hungarian Academy of Sciences |
And 4 more authors.
Virus Genes | Year: 2013
Recently a new group of circoviruses have been detected in tissues of Barbel fish and European catfish in Hungary. In our study circovirus genomes were screened in eight additional fish species for the detection and characterization of circoviruses. Two species of these bore circoviral sequences based on conventional PCR assay targeting the replication-associated protein coding gene fragments. Interestingly, the methods successfully used before failed to amplify other parts of the circular viral genome, suggesting the presence of partial, integrated genetic elements in the genome of the host. The successfully sequenced fragments of the Indian rohu (Labeo rohita) encoded mutations which may cause frameshifts or termination in the coding region described previously in other vertebrates. Phylogenetic analyses presumed that integration of the viral genetic elements might have progressed concurrently or following the diversification of cyprinid fish. Further studies on the nature of whole circovirus genomes and integrated elements may help to understand their potential role and evolution in different fish species. © 2013 Springer Science+Business Media New York.
Ahmad F.,University of Delhi |
Singh D.,University of Delhi |
Arya P.V.,University of Delhi |
Singh H.S.,Ch iversity
Biochemical and Cellular Archives | Year: 2015
Class Monogenea attracted lot of attention for phylogenetic study in recent past. Family Ancyrocephalidae present a good material to employ in silico tools. Present study is an attempt to employ 28S rRNA information in predicting phylogeny of the different members. Another approach of integrating geo-mapping is also attempted for understanding diversity, distribution and relatedness among the various members of the family under the study.
Tiwari A.K.,Ch iversity |
Bharti Y.,Ch iversity |
Tripathi S.,Ch iversity |
Mishra N.,Ch iversity |
And 5 more authors.
Acta Phytopathologica et Entomologica Hungarica | Year: 2010
Research on sugarcane biotechnology began in the 1960s with in vitro culture. Serious efforts to improve sugarcane crops by molecular approaches have commenced only in the past two decades. There is an increasing pressure worldwide to enhance the productivity of sugarcane in order to sustain profitable sugar industries, while there are several diseases attacking sugarcane and reducing the quality of the crop. Biotechnological approaches for sugarcane improvement have been applied in the areas of: (1) cell and tissue culture for rapid propagation genetic transformation and molecular breeding, (2) engineering novel genes into commercial cultivars, (3) molecular diagnostics of sugarcane pathogens, (4) developing genetic maps using molecular marker technology, (5) understanding the molecular basis of sucrose accumulation in the stem, (6) molecular testing of plants for clonal fidelity, (7) variety identification and (8) molecular characterization of various traits. Most of the current research in sugarcane biotechnology is recently focused primarily on transgenic and marker assisted breeding. Advancements have made it possible to sequence the complete genome of increasingly complex organisms and to clone and transfer individual genes to engineer new traits.