Cg Bhakta Institute Of Biotechnology

Tarsadi, India

Cg Bhakta Institute Of Biotechnology

Tarsadi, India
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Jain V.C.,Ck Pithawalla Institute Of Pharmaceutical Science And Research | Shah D.P.,Ck Pithawalla Institute Of Pharmaceutical Science And Research | Patel P.K.,Ck Pithawalla Institute Of Pharmaceutical Science And Research | Joshi B.H.,Cg Bhakta Institute Of Biotechnology
Global Journal of Pharmacology | Year: 2010

The development of bacterial resistance to presently available antibiotics has necessitated the search of new antibacterial agents. Plants and plant products are known to possess excellent antioxidant properties and play a significant role in preventing the conditions due to the excessive free radicals. The present study was aimed to evaluate the antimicrobial and antioxidant activities of the alcoholic and aqueous extracts from the root of Alangium salvifolium (L.f) Wang. Aqueous and alcoholic extracts prepared and were tested on Gram positive and Gram negative bacteria. Agar cup plate test was used to determine the sensitivity of the tested samples while the well micro-dilution was used to determine the minimum inhibitory concentration. The DPPH and Nitric oxide radical inhibiting activity were used to detect oxidative activity. The results of antimicrobial assays showed that all tested extract were active against all tested microbial species including gram positive and negative bacteria. The alcoholic extract showed direct antimicrobial activity against all tested microorganism with minimum inhibitory concentration ranging between 0.130 to 0.520 mg/ml, while aqueous extract showed 0.26 to 2.10 mg/ml, respectively. The results of DPPH method showed 50% inhibition rate at the 120.48μg/ml and 135.14 μg/ml with alcoholic and aqueous extract, respectively. Nitric oxide scavenging inhibition showed 50% inhibition rate at the 308.80 μg/ml and 450.80 μg/ml using alcoholic and aqueous extract, respectively. The overall results of this study indicates that the extract from roots have interesting antimicrobial and potential free radical scavenging activity for treatment of diseases. © IDOSI Publications, 2010.

Desai K.G.,Macleods Pharmaceuticals Ltd | Naik J.I.,West Corporation | Raval J.P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Desai K.R.,Cg Bhakta Institute Of Biotechnology
Arabian Journal of Chemistry | Year: 2014

Heterocyclic compounds containing pyrazolines were reported to possess significant biological activity. Synthesis of 2-(ω-chloroacetonyl)-3-p-fluorophenyl-6-bromoquinazoline-4(3H)-ones (2), 2-(ω-hydrazinoacetonyl)-3-p-fluorophenyl-6-bromoquinazoline-4(3H)-ones (3) and 1'-[3H-3-p-fluorophenyl-4-oxo-6-bromoquinazoline-2-acetonyl]-3'-[1-o-chlorophenyl-3-methyl-5-azomethine-2-pyrazolidene]-5'-(substituted phenyl)-δ2-pyrazolines (4a-j) have been described. Some of the new compounds were tested against bacteria (Gram -ve and Gram +ve) and fungi. © 2011.

Rana A.M.,Sardar Vallabhbhai National Institute of Technology, Surat | Desai K.R.,Cg Bhakta Institute Of Biotechnology | Jauhari S.,Sardar Vallabhbhai National Institute of Technology, Surat
Research on Chemical Intermediates | Year: 2014

To investigate the antimicrobial properties of the rhodanine (2-thioxo-4-thiazolidinone) structure, several 2-[(5Z)-5-benzylidene-4-oxo-2- thioxo-1,3-thiazolidin-3-yl]-N-phenylacetamide derivatives were synthesized by use of an efficient procedure. Variation of the functional group on the 5-benzylidine ring of rhodanine led to compounds containing a 2-thioxo-4-thiazolidinone group attached to N-phenyl acetamide. The chemical structures of the compounds were confirmed by IR, 1H NMR, and 13C NMR spectroscopy, ESI mass spectrometry, and elemental analysis. The antibacterial and antifungal activity of the compounds were tested, at seven concentrations, against Gram-positive bacterial strains (Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922), Gram-negative bacterial strains (Staphylococcus aureus ATCC 25923 and Bacillus subtilis ATCC 11774), and fungal strains (Candida albicans ATCC 66027 and Aspergillus niger ATCC 6275), by use of the Kirby Bauer disk-diffusion technique and the serial broth dilution technique. The results obtained were compared with those for reference drugs. Relationships between structure and their antimicrobial activity are discussed. © 2013 Springer Science+Business Media Dordrecht.

Selvapandiyan A.,Institute of Molecular Medicine | Dey R.,CBER | Gannavaram S.,CBER | Solanki S.,Institute of Molecular Medicine | And 3 more authors.
Vaccine | Year: 2014

Visceral leishmaniasis (VL) is fatal if not treated and is prevalent widely in the tropical and sub-tropical regions of world. VL is caused by the protozoan parasite Leishmania donovani or Leishmania infantum. Although several second generation vaccines have been licensed to protect dogs against VL, there are no effective vaccines against human VL [1]. Since people cured of leishmaniasis develop lifelong protection, development of live attenuated Leishmania parasites as vaccines, which can have controlled infection, may be a close surrogate to leishmanization. This can be achieved by deletion of genes involved in the regulation of growth and/or virulence of the parasite. Such mutant parasites generally do not revert to virulence in animal models even under conditions of induced immune suppression due to complete deletion of the essential gene(s). In the Leishmania life cycle, the intracellular amastigote form is the virulent form and causes disease in the mammalian hosts. We developed centrin gene deleted L. donovani parasites that displayed attenuated growth only in the amastigote stage and were found safe and efficacious against virulent challenge in the experimental animal models. Thus, targeting genes differentially expressed in the amastigote stage would potentially attenuate only the amastigote stage and hence controlled infectivity may be effective in developing immunity. This review lays out the strategies for attenuation of the growth of the amastigote form of Leishmania for use as live vaccine against leishmaniasis, with a focus on visceral leishmaniasis. © 2014 Elsevier Ltd.

Raval J.P.,Ashok and Rita Patel Institute of Integrated Study | Desai K.G.,Veer Narmad South Gujarat University | Desai K.R.,Cg Bhakta Institute Of Biotechnology
Journal of Saudi Chemical Society | Year: 2012

Heterocyclic compounds containing pyrazolyl-oxopropyl-quinazolin-4(3H)-one are reported to possess significant biological activity. Syntheses of 6-bromo-2-(3-chloro-2-oxopropyl)-3-(4-fluorophenyl)quinazolin-4(3H)-one 2 6-bromo-3-(4-fluorophenyl)-2-(3-hydrazinyl-2-oxopropyl)quinazolin-4(3H)-one 3 and 6-bromo-2-(3-(3-(4-(1-(2-chlorophenyl)-3-methyl-1H-pyrazol-5(4H)-ylideneamino)phenyl)-5-(substituted phenyl)-4,5-dihydro-1H-pyrazol-1-yl)-2-oxopropyl)-3-(4-fluorophenyl)quinazolin-4(3H)-one 5a-j using microwave irradiation have been described. These compounds have been characterized on the basis of the UV, IR, 1H NMR, 13C NMR, Mass and elemental analysis. Compounds have been evaluated for their antimicrobial activity. © 2011.

Parmar P.,Cg Bhakta Institute Of Biotechnology | Soni A.,Veer Narmad South Gujarat University | Vyas K.,Gujarat University | Desai P.V.,Veer Narmad South Gujarat University
Agris On-line Papers in Economics and Informatics | Year: 2012

The impact of industrialization on the quality of environment is evident and there is need to have eco-friendly strategies to treat effluent for the sustainable development of industries. Treatment with the help of microorganisms can effectively be used to reduce the load of harmful chemicals in the environment. Study on bioremediation of cyanide released in the effluent has been carried out by the microbes, isolated from soil collected from different industrial contaminated sites. Microbes are enriched in Bushnell-Haas medium containing 100ppm filter sterilized potassium cyanide which has converted it into less toxic products such as ammonia, CO2, formate and formamide. Three distinct groups of bacteria were isolated which are able to degrade cyanide present in the simulated media. Conditions regarding the pH, maximum concentration of cyanide, dry weight of microbes and presence of ammonia of the simulated effluent were optimized favoring the growth of cyanide degrading bacteria. The optimized conditions can be replicated while utilizing these microbes in the bioremediation studies of cyanide contaminated sites. Morphological, cultural, biochemical and 16S rDNA characterization of isolated bacterial cells has shown that Pseudomonas, Brucella and Ochrobacterum bacterial species are responsible for degrading cyanide. © 2010.

Patel M.,Cg Bhakta Institute Of Biotechnology | Baxi K.,Indian Central Salt and Marine Chemicals Research Institute | Dayma P.,Indian Central Salt and Marine Chemicals Research Institute | Upadhyay D.,Indian Central Salt and Marine Chemicals Research Institute | And 5 more authors.
Clean - Soil, Air, Water | Year: 2014

The present work aimed to make an assessment of physicochemical parameters and bacteriological contamination associated with 24 ground water resources used by more than 5% of total population, classified under four characteristic locations of Bhavnagar, a growing city of Gujarat. Ground water quality was evaluated with respect to most probable number of Escherichia coli, and presence of heterotrophic bacteria including some pathogens. Based on morphological differences and sites, 27 isolates were randomly selected and identified accurately. Scanning electron microscopy was also performed for each representative genus to understand morphology. Resources from thickly crowded areas were dominated by Aeromonas and Acinetobacter, residential area by Citrobacter and Serratia and sites adjacent to drainage canals by Pseudomonas spp. Further, bacterial community structure of 24 different resources was determined by Biolog EcoPlates™ and species richness and diversity were evaluated. Antibiotic resistance pattern revealed emergence of multidrug resistance among some of the identified strains. To the best of our knowledge, this is the first report on detailed bacteriological analysis of ground water resources of Bhavnagar city including bacterial identification using polyphasic approach, community study, antibiotics resistance profiling, and interpretation of data using suitable statistical tools. Furthermore, this study can be considered as a model for understanding ground water quality of other cities also. Groundwater quality of Bhavnagar, India was evaluated with respect to bacterial contamination. Resources from thickly crowded areas were dominated by Aeromonas and Acinetobacter, residential area by Citrobacter and Serratia, and sites adjacent to drainage canals by Pseudomonas spp. Antibiotic resistance pattern revealed presence of multidrug-resistant bacteria. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Lokhandwala S.,Institute of Chemical Technology | Desai K.R.,Cg Bhakta Institute Of Biotechnology
Asian Journal of Chemistry | Year: 2013

A new family of organophosphorus compounds namely 3-chloro-1-(10-hydroxy- 10-oxo-5,10-dihydro-phenophosphazin-2-yl)-4-(substituted phenyl)-azetidin-2-one (3a-j) has been synthesized by reaction of 2-[(substituted benzylidene)-amino]- 10-oxo-5,10-dihydro-phenophosphazin-10-ol (2a-j) with chloro acetyl chloride and triethyl amine. All these compounds were characterized by elemental and spectral analysis. Their antimicrobial activity was also evaluated. Copyright © 2013 Asian Journal of Chemistry.

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