Manohara A.,Field Marshal K M Cariappa College |
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2011
Glucoamylases are one of the amylolytic enzymes used in starch hydrolysis in food industry. Major enzymes of industrial importance are of microbial origin. Glucoamylases are of a family of Endoamylases that catalysis both 1,4 and 1,6- glycosidic linkage in polymers of a D-glucose. So it increases the yield but is used after initial treatment with α-amylase. The amylolytic yeasts that are potential candidates that can reduce the cost of production. In the present work, several strains of yeasts were screened from rotten potato and rotten sweet potato and tested for efficiency of starch hydrolysis. Quantitative determinations of amylolytic potentiality of the yeasts were determined by assay method. Different parameters like pH, starch concentration and temperature required for maximum glucoamylase production were tested. These screened yeast strains were identified by microscopic and biochemical parameters. Totally four different strains of yeasts were isolated from rotten potato and rotten sweet potato are identified as Cystofilabasidium sp, Filobasidium Sp, Saccharomycopsis Sp and Cryptococcus Sp. © Global Science Publications.
Isolation, partial purification of protein and detection of antibacterial activity in leaf extracts of Tephrosia cinerea (L) Pers. - An ethno-medicinal plant practiced by tribal community at biligirirangana hills of Karnataka, india
Panduranga Murthy G.,Shridevi Institute of Engineering and Technology |
Mokshith M.C.,CFTRI |
Ravishankar H.G.,Shridevi Institute of Engineering and Technology
International Journal of Pharma and Bio Sciences | Year: 2011
Aqueous and Organic solvent extracts of Tephrosia cinerea, (L) Pers. an ethno-medicinal plant practiced by Tribal (Soliga) community against wound healing at Biligirirangana Hills of Karnataka have been evaluated for its bioactivity. The leaves were used to isolate and purify Proteins and ammonium sulphate fractionation yield 2.4 gm/ml. A single prominent peak was obtained on sephadex G-75 column with fraction 6 th showing maximum bioactivity. TLC of ethyl acetate, acetone and petroleum ether extracts yielded three spots with Rf value of 0.85. The native Page revealed that, the presence of four bands and their positions suggested that, the protein present appears to be medium to low molecular size. However, the molecular characterization of the purified protein was very much essential to ascertain the nature of the same. Antimicrobial activity of organic solvent extracts, such as Acetone, Ethyl acetate and Petroleum ether were tested against pathogenic bacteria i.e., Gram +ve (E.coli) and Gram -ve (Pseudomonas aeruginosa) which were isolated from infected patients of the authorized hospital. The result reveals that, both Acetone (7.2mm) and Ethyl acetate (6.3mm) showed significant zone of inhibition against P. aeruginosa. But, Petroleum ether did not show any positive activity against P. aeruginosa. Similarly, both Ethyl acetate and Petroleum ether did not show activity against E. coli, whereas, Acetone extract of T. cinerea inhibited the growth of E.coli (9mm) and are significantly superior over other solvent extracts. Besides, the total content of phenolics in the sample was found to be 212.5 mg/ml. Hence, the obtained result suggests, susceptibility of both bacterial strains to the organic as well as purified extracts of tested ethno-medicinal plant. In addition, this plant would also exert several beneficial effects by virtue of its antibacterial activity and could be harnessed as drug formulation based on the tribal practice. The further investigation however is required to ascertain these in vitro results which are attainable in vivo through biophysical characterization of isolated bioactive constituents after complete purification.
Pednekar M.,Bhabha Atomic Research Center |
Das A.K.,CFTRI |
Rajalakshmi V.,Bhabha Atomic Research Center |
Sharma A.,Bhabha Atomic Research Center
Radiation Physics and Chemistry | Year: 2010
Effect of radiation processing (10, 20 and 30 kGy) on soybean for better utilization was studied. Radiation processing reduced the cooking time of soybean and increased the oil absorption capacity of soy flour without affecting its proximate composition. Irradiation improved the functional properties like solubility, emulsification activity and foam stability of soybean protein isolate. The value addition effect of radiation processing has been discussed for the products (soy milk, tofu and tofu fortified patties) prepared from soybean. © 2009.
Chetana P.R.,Bangalore University |
Rao R.,Bangalore University |
Saha S.,Indian Institute of Science |
Policegoudra R.S.,Defense Research Laboratory |
Polyhedron | Year: 2012
New ternary copper (II) complexes, [Cu(l-orn)(B)(Cl)](Cl·2H 2O) (1-2) where l-orn is l-ornithine, B is an N,N-donor heterocyclic base, viz. 2,2′-bipyridine (bpy, 1) and 1,10-phenanthroline (phen, 2), were synthesized and characterized by various spectroscopic techniques. Complex 2 is characterized by the X-ray single crystallographic method. The complex shows a distorted square-pyramidal (4 + 1) CuN 3OCl coordination sphere. Binding interactions of the complexes with calf thymus DNA (CT-DNA) were investigated by UV-Vis absorption titration, ethidium bromide displacement assay, viscometric titration experiment and DNA melting studies. Complex 2 shows appreciable chemical nuclease activity in the presence of 3-mercaptopropionic acid (MPA). The complexes were subjected to in vitro cytotoxicity studies against carcinomic human alveolar basal epithelial cells (A-549) and human epithelial (HEp-2) cells. The IC 50 values of 1 and 2 are less than that of cisplatin against HEp-2 cell lines. MIC values for 1 against the bacterial strains Streptococcus mutans and Pseudomonas aeruginosa are 0.5 mM. © 2012 Elsevier Ltd. All rights reserved.
Manohara A.,FMKMC College |
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2010
Amylase is one of the most important enzyme used in Biotechnology, particularly in processing starch hydrolysis in food industry. Enzymes are Protein with highly specialized calalytic function produced by all living organisms including micro organisms. Amylolytic yeasts play a significant role in starch degradation. The capacity to degrade starch differs with different species. In the present work, several strains of yeast were isolated from rotten sweet potato and rotten potato used to screen for their efficiency to hydrolyse starch. Qualitative and quantitative determination of amylolytic potentiality of the yeasts were determined by assay method. Different parameters like pH, starch concentration and temperature requirement for maximum amylase production were optimized. Amylase Positive yeast strains were identified by microscopic and biochemical parameters. Four strains of yeasts of different genus were isolated from rotten sweet potato and rotten potato with higher amylase degrading capacity. Cystofilobasidium sp Filobasidium sp, Saccharomycopsis sp, and Cryptococcus sp are the different strains isolated. © Global Science Publications.