CEVA PHYLAXIA Veterinary Biologicals Co.

Budapest, Hungary

CEVA PHYLAXIA Veterinary Biologicals Co.

Budapest, Hungary
SEARCH FILTERS
Time filter
Source Type

PubMed | University of Pécs, Veterinary Diagnostic Directorate, Hungarian Academy of Sciences, Ceva Phylaxia Veterinary Biologicals Co. and United Szent Istvan Szent Laszlo Hospital Clinic
Type: Journal Article | Journal: Genome announcements | Year: 2015

We have investigated the genomic properties of three turkey reovirus strains-19831M09, D1246, and D1104-isolated in Hungary in 2009. Sequence identity values and phylogenetic calculations indicated genetic conservativeness among the studied Hungarian strains and a close relationship with strains isolated in the United States.


Banyai K.,Hungarian Academy of Sciences | Dandar E.,Hungarian Academy of Sciences | Dorsey K.M.,CEVA BIOMUNE | Mato T.,CEVA PHYLAXIA Veterinary Biologicals Co. | Palya V.,CEVA PHYLAXIA Veterinary Biologicals Co.
Virus Genes | Year: 2011

Avian orthoreoviruses (ARVs) are responsible for considerable economic losses in broiler chickens; yet, the genetic characterization of most ARV strains is limited to a few genes, and the full coding region has been determined for only S1133 and 138, two ARV strains associated with tenosynovitis. Recently, in parts of the United States, ARVs with novel neutralization antigen type were isolated from chickens afflicted with runting-stunting syndrome. One such strain, AVS-B, was selected for full genome sequencing and phylogenetic analysis. The complete genome was 23,494 bp in size and included 12 open reading frames. The lengths of the coding regions, as well as those of the 50 and 30 ends, were fairly well conserved between AVS-B and other reference strains. In pairwise comparisons to the S1133 and 138 strains, the AVS-B strain shared a wide range of sequence identities along each genome segment, i.e., a range of 54-55% for the σC coding region of S1 genome segment and 91-93% for the S2 genome segment. Phylogenetic analyses of individual genes of AVS-B did not identify any single common ancestor among more completely characterized ARV strains for which sequence data are available. One exception to this lack of identity was strain 138, which shared 90-93% nt identity with AVS-B along seven of ten genome segments; only M2, M3, and S1 segments of these strains shared lower sequence identities. Collectively, our analyses indicated that multiple reassortment events and strong divergence caused by the accumulation of point mutations could have led to the observed assortment and genetic heterogeneity of the AVS-B genome. © Springer Science+Business Media, LLC 2010.


Palya V.,Ceva Phylaxia Veterinary Biologicals Co. | Kiss I.,Ceva Phylaxia Veterinary Biologicals Co. | Tatar-Kis T.,Ceva Phylaxia Veterinary Biologicals Co. | Mato T.,Ceva Phylaxia Veterinary Biologicals Co. | And 2 more authors.
Avian Diseases | Year: 2012

Newcastle disease (ND) is a highly contagious disease of chickens causing significant economic losses worldwide. Due to the limitation in their efficacy, current vaccination strategies against ND need improvements. This study aimed to evaluate a new-generation ND vaccine for its efficacy in providing clinical protection and reducing virus shedding after challenge. Broiler chickens were vaccinated in ovo or subcutaneously at hatch with a turkey herpesvirus-based recombinant vaccine (rHVT) expressing a key protective antigen (F glycoprotein) of Newcastle disease virus (NDV). Groups of birds were challenged at 20, 27, and 40 days of age with a genotype V viscerotropic velogenic NDV strain. Protection was 57 and 81, 100 and 95, and 100 and 100 after the subsequent challenges in the in ovo and subcutaneously vaccinated chickens, respectively. Humoral immune response to vaccination could be detected from 34 wk of age. Challenge virus shedding was lower and gradually decreased over time in the vaccinated birds compared to the unvaccinated control chickens. In spite of the phylogenetic distance between the NDV F gene inserted into the vector vaccine and the challenge virus (genotype I and V, respectively), the rHVT NDV vaccine provided good clinical protection and significantly reduced challenge virus shedding. © American Association of Avian Pathologists.


Kugler R.,Hungarian Academy of Sciences | Dandar E.,United Szent Istvan es Szent Laszlo Hospital Clinic | Feher E.,Hungarian Academy of Sciences | Jakab F.,University of Pécs | And 4 more authors.
Virus Research | Year: 2016

Avian orthoreoviruses cause various diseases in wild birds and domesticated poultry. In this study we report the detection and genomic characterization of a partridge (Perdix perdix) origin reovirus strain, D1007/2008. The virus was isolated on cell culture from acute pneumonia and infra-orbital sinusitis. The 23,497 nucleotide long genome sequence was obtained by combined use of semiconductor and capillary sequencing. Sequence and phylogenetic analyses showed that the partridge reovirus strain was related to orthoreoviruses of gallinaceous birds. In fact, five (λB, λC, μB, σC, σNS) and one (σB) out of 10 genes clustered definitely with turkey or chicken origin orthoreoviruses, respectively, whereas in the λA, μA, μNS and σA phylogenies a more distant genetic relationship was observed. Our data indicate that the identified reovirus strain is composed of a mixture of chicken and turkey orthoreovirus related alleles. This finding implies that partridges may serve as natural reservoirs for orthoreoviruses of domesticated poultry. © 2015 Elsevier B.V.


Sutton K.M.C.,Roslin Institute | Hu T.,Roslin Institute | Wu Z.,Roslin Institute | Siklodi B.,CEVA Phylaxia Veterinary Biologicals Co. | And 2 more authors.
Developmental and Comparative Immunology | Year: 2015

A new member of the chicken TNF superfamily has recently been identified, namely receptor activator of NF-κB ligand (RANKL), as have its signalling receptor, RANK, and its decoy receptor, osteoprotegerin (OPG). In mammals, RANKL and RANK are transmembrane proteins expressed on the surface of Th1 cells and dendritic cells (DC) respectively, whereas OPG is expressed as a soluble protein from osteoblasts and DC. Recombinant soluble chicken RANKL (chRANKL) forms homotrimers whereas chicken OPG (chOPG) forms homodimers, characteristic of these molecules in mammals. ChRANKL, chRANK and chOPG are expressed at the mRNA level in most tissues and organs. ChRANKL is transcriptionally regulated by Ca2+ mobilisation and enhances the mRNA expression levels of pro-inflammatory cytokines in bone marrow-derived DC (BMDC); this is inhibited by both chOPG-Fc and soluble chRANK-Fc. However, chRANKL does not enhance the expression of cell surface markers in either BMDC or BM-derived macrophages (BMM). Furthermore, chRANKL enhances the survival of APC similar to its mammalian orthologue. © 2015.


Jordan I.,ProBioGen AG | John K.,ProBioGen AG | Howing K.,ProBioGen AG | Lohr V.,ProBioGen AG | And 7 more authors.
Avian Pathology | Year: 2016

Veterinary vaccines contribute to food security, interrupt zoonotic transmissions, and help to maintain overall health in livestock. Although vaccines are usually cost-effective, their adoption depends on a multitude of factors. Because poultry vaccines are usually given to birds with a short life span, very low production cost per dose is one important challenge. Other hurdles are to ensure a consistent and reliable supply of very large number of doses, and to have flexible production processes to accommodate a range of different pathogens and dosage requirements. Most poultry vaccines are currently being produced on primary avian cells derived from chicken or waterfowl embryos. This production system is associated with high costs, logistic complexities, rigid intervals between harvest and production, and supply limitations. We investigated whether the continuous cell lines Cairina retina and CR.pIX may provide a substrate independent of primary cell cultures or embryonated eggs. Viruses examined for replication in these cell lines are strains associated with, or contained in vaccines against egg drop syndrome, Marek's disease, Newcastle disease, avian influenza, infectious bursal disease and Derzsy's disease. Each of the tested viruses required the development of unique conditions for replication that are described here and can be used to generate material for in vivo efficacy studies and to accelerate transfer of the processes to larger production volumes. © 2016 Houghton Trust Ltd.


Dandar E.,Hungarian Academy of Sciences | Farkas S.L.,Hungarian Academy of Sciences | Marton S.,Hungarian Academy of Sciences | Oldal M.,University of Pécs | And 4 more authors.
Archives of Virology | Year: 2014

The complete genomic sequence of a Hungarian goose orthoreovirus strain (D20/99) is reported in this study. The genome of D20/99 is 22,969 bp in length (range, 3958 bp for L1 to 1124 bp for S4) and encodes 11 putative proteins. Pairwise sequence comparisons and phylogenetic analyses indicated that D20/99 shares genetic signatures with some contemporary Chinese duck and goose reovirus strains, except for the μA, μNS and σA protein coding genes, which represented independent genetic lineages. This study implies a greater genetic diversity among waterfowl-origin orthoreoviruses than hitherto recognized. © 2014 Springer-Verlag Wien.


PubMed | University of Pécs, Indian Veterinary Research Institute, Hungarian Academy of Sciences, Ceva Phylaxia Veterinary Biologicals Co. and University of Bari
Type: Journal Article | Journal: Biologicals : journal of the International Association of Biological Standardization | Year: 2014

The laboratory rotavirus strain, BRS/115, has been used for more than two decades to monitor rotaviruses in specific pathogen free flocks of laying hens. However, the virus strain has not been characterized in detail. Therefore we aimed at the description of molecular features of BRS115 by using random primed reverse transcription-PCR of the genomic RNA followed by massive parallel sequencing using the semiconductor sequencing technology. Over 64,000 trimmed reads mapped to reference sequences obtained from GenBank. The strain classified into the species Rotavirus A and genotyped G7-P[35]-I4-R4-C4-M4-A16-N4-T4-E11-H4 according to guidelines of the Rotavirus Classification Working Group. Phylogenetic analysis identified shared features with chicken, turkey and pigeon origin rotaviruses. This study demonstrates the robustness of next generation sequencing in the characterization of reference virus materials used in specialized laboratories.


PubMed | Roslin Institute and CEVA Phylaxia Veterinary Biologicals Co.
Type: Journal Article | Journal: Developmental and comparative immunology | Year: 2015

A new member of the chicken TNF superfamily has recently been identified, namely receptor activator of NF-B ligand (RANKL), as have its signalling receptor, RANK, and its decoy receptor, osteoprotegerin (OPG). In mammals, RANKL and RANK are transmembrane proteins expressed on the surface of Th1 cells and dendritic cells (DC) respectively, whereas OPG is expressed as a soluble protein from osteoblasts and DC. Recombinant soluble chicken RANKL (chRANKL) forms homotrimers whereas chicken OPG (chOPG) forms homodimers, characteristic of these molecules in mammals. ChRANKL, chRANK and chOPG are expressed at the mRNA level in most tissues and organs. ChRANKL is transcriptionally regulated by Ca(2+) mobilisation and enhances the mRNA expression levels of pro-inflammatory cytokines in bone marrow-derived DC (BMDC); this is inhibited by both chOPG-Fc and soluble chRANK-Fc. However, chRANKL does not enhance the expression of cell surface markers in either BMDC or BM-derived macrophages (BMM). Furthermore, chRANKL enhances the survival of APC similar to its mammalian orthologue.


PubMed | United Szent Istvan es Szent Laszlo Hospital Clinic, University of Pécs, Hungarian Academy of Sciences and Ceva Phylaxia Veterinary Biologicals Co.
Type: | Journal: Virus research | Year: 2016

Avian orthoreoviruses cause various diseases in wild birds and domesticated poultry. In this study we report the detection and genomic characterization of a partridge (Perdix perdix) origin reovirus strain, D1007/2008. The virus was isolated on cell culture from acute pneumonia and infra-orbital sinusitis. The 23,497 nucleotide long genome sequence was obtained by combined use of semiconductor and capillary sequencing. Sequence and phylogenetic analyses showed that the partridge reovirus strain was related to orthoreoviruses of gallinaceous birds. In fact, five (B, C, B, C, NS) and one (B) out of 10 genes clustered definitely with turkey or chicken origin orthoreoviruses, respectively, whereas in the A, A, NS and A phylogenies a more distant genetic relationship was observed. Our data indicate that the identified reovirus strain is composed of a mixture of chicken and turkey orthoreovirus related alleles. This finding implies that partridges may serve as natural reservoirs for orthoreoviruses of domesticated poultry.

Loading CEVA PHYLAXIA Veterinary Biologicals Co. collaborators
Loading CEVA PHYLAXIA Veterinary Biologicals Co. collaborators