Entity

Time filter

Source Type


Panjwani D.,University of Central Florida | Yesiltas M.,University of Central Florida | Nath J.,University of Central Florida | Maukonen D.E.,University of Central Florida | And 9 more authors.
Infrared Physics and Technology | Year: 2014

A method to pattern infrared-absorbing gold black by conventional photolithography and lift-off is described. A photo-resist pattern is developed on a substrate by standard photolithography. Gold black is deposited over the whole by thermal evaporation in an inert gas at ∼1 Torr. SiO2 is then deposited as a protection layer by electron beam evaporation. Lift-off proceeds by dissolving the photoresist in acetone. The resulting sub-millimeter size gold black patterns that remain on the substrate retain high infrared absorption out to ∼5 μm wavelength and exhibit good mechanical stability. This technique allows selective application of gold black coatings to the pixels of thermal infrared imaging array detectors. © 2013 Elsevier B.V. All rights reserved. Source


Paprotka T.,University of Stuttgart | Paprotka T.,ACT Biotech | Deuschle K.,University of Stuttgart | Pilartz M.,University of Stuttgart | And 2 more authors.
Virus Research | Year: 2015

A comprehensive survey on the viral minichromosomes of the begomoviruses Abutilon mosaic virus, tomato yellow leaf curl Sardinia virus, African cassava mosaic virus, Indian cassava mosaic virus (family Geminiviridae) during the course of infections in Nicotiana benthamiana is summarized. Using optimized one-dimensional and two-dimensional gel systems combined with blot hybridization and a standardized evaluation, discrete and heterogeneous virus-specific signals with different DNA forms were compared to trace functions of viral multiplication with inactive/active replication and/or transcription. A quantitative approach to compare the distantly related viruses during the course of infection with the aim to generalize the conclusions for geminiviruses has been developed. Focussing on the distribution of topoisomers of viral supercoiled DNA, which reflect minichromosomal stages, predominant minichromosomes with 12 nucleosomes, less with 13 nucleosomes and no with 11 nucleosomes were found. These results indicate that chromatin with only one open gap to bind transcription factors is the favourite form. The dynamics during infections in dependence on the experimental conditions is discussed with reference to the design of experiments for resistance breeding and molecular analyses. © 2014 Elsevier B.V. Source


Unger M.,University of Wisconsin - Milwaukee | Unger M.,CETICS Healthcare Technologies GmbH | Sedlmair J.,U.S. Department of Agriculture | Siesler H.W.,University of Duisburg - Essen | Hirschmugl C.,University of Wisconsin - Milwaukee
Vibrational Spectroscopy | Year: 2014

In the present study, 3D FT-IR spectroscopic imaging measurements were applied to study the phase separation of a poly(3-hydroxybutyrate) (PHB)/poly(l-lactic acid) (PLA) (50:50 wt.%) polymer blend film. While in 2D projection imaging the z-dependent information is overlapped, thereby complicating the analysis, FT-IR spectro-micro-tomography, obtained from computed tomographic back projection calculations, results in distinct 3D chemical images that provide detailed information of phase separation of the two polymer components that are well separated. © 2014 Elsevier B.V. All rights reserved. Source


Unger M.,CETICS Healthcare Technologies GmbH | Koster H.,CETICS Healthcare Technologies GmbH | Popescu A.,BioTeSys | Pfitzner I.,BioTeSys
BioSpektrum | Year: 2014

Cell culture has become one of the major tools in life science today. In addition to the study of cell functions for fundamental research, the use of cell lines for the production of pharmaceutical substances is of great importance. Evaluating the general health of a cell culture is usually based on different cell characteristics. Mid-infrared spectroscopy in combination with chemometric methods proves to be highly suited for this purpose in terms of reproducibility, objectivity, cost-effectiveness and speed. © 2014, Springer-Verlag Berlin Heidelberg. Source


Unger M.,CETICS Healthcare Technologies GmbH | Koster H.,CETICS Healthcare Technologies GmbH | Popescu A.,BioTeSys | Pfitzner I.,BioTeSys
BioSpektrum | Year: 2014

Cell culture has become one of the major tools in life science today. In addition to the study of cell functions for fundamental research, the use of cell lines for the production of pharmaceutical substances is of great importance. Evaluating the general health of a cell culture is usually based on different cell characteristics. Mid-infrared spectroscopy in combination with chemometric methods proves to be highly suited for this purpose in terms of reproducibility, objectivity, cost-effectiveness and speed. © 2014, Springer-Verlag Berlin Heidelberg. Source

Discover hidden collaborations