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Carneiro P.A.,Sao Paulo State University | Umbuzeiro G.A.,CETESB Cia de Tecnologia de Saneamento Ambiental | Oliveira D.P.,Toxicologicas e Bromatologicas | Zanoni M.V.B.,Sao Paulo State University
Journal of Hazardous Materials | Year: 2010

High performance liquid chromatography coupled to a diode array detector method was developed to detect disperse dyes in water samples over the range 0.50-35 ng, with detection limits of 0.09 ng, 0.84 ng and 0.08 ng, respectively, with good repeatability and accuracy. This study identifies the disperse azo dyes C.I. Disperse Blue 373, C.I. Disperse Orange 37 and Disperse Violet 93 as components of a commercial dye formulation assigned as Dispersol Black Dye (CVS) used in the textile industry for dyeing synthetic fibers that are contributing to the mutagenicity found in the Cristais River, São Paulo, Brazil. High performance liquid chromatography coupled to a diode array detector was applied to monitor the occurrence of these dyes in: (1) the treated industrial effluent, (2) raw river water, (3) treated river water, and (4) the sludge produced by a Drinking Water Treatment Plant (DWTP) which is located 6 km downstream from the textile industrial discharge, where dyes' concentrations changed from 1.65 ng L-1 to 316 μ L-1. © 2009 Elsevier B.V. All rights reserved. Source

Umbuzeiro G.D.A.,CETESB Cia de Tecnologia de Saneamento Ambiental | Umbuzeiro G.D.A.,University of Sao Paulo | Rech C.M.,CETESB Cia de Tecnologia de Saneamento Ambiental | Correia S.,CETESB Cia de Tecnologia de Saneamento Ambiental | And 5 more authors.
Environmental and Molecular Mutagenesis | Year: 2010

The objective of this study was to compare the responses of the Salmonella/microsome microsuspension assay with the new microplate fluctuation protocol (MPF) for the evaluation of the mutagenic activity of environmental samples. Organic extracts of total particulate atmospheric air samples, surface waters, and effluents were tested in dose-response experiments. The assays were performed with strain TA98 in the absence and presence of S9 mix. Both protocols produced similar results, despite the fact that the maximum score of the MPF is limited to 48 wells, whereas in the regular plate assay it is possible to count up to 1,500 colonies using an automatic counter. Similar sensitivities based on the lowest dose that resulted in a positive response were obtained for both assays. The MPF procedure is less laborious (e.g., all-liquid format, use of multichannel pipettors) and allows for automation of the pipetting and dispensing steps, thus, reducing time of the analysis which is particularly important in environmental quality monitoring programs or in effect-directed analysis. The results show that the MPF procedure is a promising tool to test environmental samples for mutagenic activity. © 2009 Wiley-Liss, Inc. Source

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