Certis Europe

Saronno, Italy

Certis Europe

Saronno, Italy

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Myrta A.,Certis Europe | Palmisano F.,CNR Institute of Plant virology | Pulaj B.,Intercooperation Horticultural Promotion in Kosovo | Susuri L.R.,Kosova Academy of science and Arts | Boscia D.,CNR Institute of Plant virology
Journal of Plant Pathology | Year: 2011

Sharka, caused by Plum pox virus (PPV), is the most destructive viral disease of plum, apricot and peach. Although PPV is widespread in all fruit-growing areas of eastern European countries, and causes serious yield losses, little is known about its occurrence and distribution in Kosovo. Therefore, a survey was conducted in orchards and nurseries at 18 sites located in seven different districts to verify the presence of PPV and to determine the virus strains occurring in the country by serological and molecular tools (ELISA and PCR). Field observations and laboratory analysis disclosed a very high incidence of PPV in nurseries and orchards at all locations. Characterization of 26 isolates representative of all surveyed sites and Prunus species, revealed a predominance of PPV-Rec (23 out of 26), and a low incidence of PPV-M and PPV-D.


Matic S.,University of Bari | Matic S.,CNR Institute of Plant virology | Myrta A.,Certis Europe | Boscia D.,CNR Institute of Plant virology | Minafra A.,CNR Institute of Plant virology
Journal of Plant Pathology | Year: 2010

Different Prunus species from Italy, Serbia, Turkey and Egypt were assayed by one-step RT-PCR and nested PCR for the presence of Plum bark necrosis stem pittingassociated virus (PBNSPaV). This virus had a fairly high incidence and was monitored throughout the year by IC-RT-PCR and ELISA. It was detected during all seasons by both techniques but most easily in spring in the new flushes of vegetation. Six PBNSPaV isolates were characterized by partial sequencing of three genomic regions, e.g. HSP70, coat protein and ORF4. High nucleotide similarity was found between all isolates from different geographic and host origins, suggesting either a relatively recent origin or the presence of unidentified constraint on variation.


Youssef K.,Egyptian Plant Pathology Research Institute | Sanzani S.M.,University of Bari | Myrta A.,Certis Europe | Ippolito A.,University of Bari
Journal of Plant Pathology | Year: 2014

The efficacy of the novel potassium bicarbonate formulation Karma (Certis Europe) for controlling Penicillium decay of orange fruit was tested. In vitro trials were carried out by amending potato dextrose agar (PDA) medium with different Karma concentrations, thereby revealing a complete inhibition of Penicillium digitatum, P. italicum, and P. ulaiense growth at 0.3, 0.3, and 0.2% (w/v), respectively. In vivo trials using dipping and spraying application strategies were conducted on Valencia late and Tarocco, two sweet orange cultivars with different degrees of susceptibility to Penicillium rot. Fruit treated with unformulated potassium bicarbonate (PB) or water served as controls. When applied by dipping, Karma and PB at 3% significantly reduced the incidence of Penicillium decay of cv. Valencia late oranges, i.e. by 79 and 31%, respectively. On the other hand, when applied by spraying, 6% Karma and PB were needed to completely inhibit decay incidence. On cv. Tarocco oranges, Karma and PB applied at 3% by dipping reduced the percentage of Penicillium decay, by a significant 87 and 68%, respectively. However, when applied by spraying at 6%, no difference was observed between the two treatments. Overall, Karma performed better than PB in controlling Penicillium rots and dipping proved to be the best application strategy.


Matic S.,University of Bari | Matic S.,CNR Institute of Plant virology | Myrta A.,Certis Europe | Minafra A.,CNR Institute of Plant virology
Journal of Plant Pathology | Year: 2010

Simultaneous detection of Plum bark necrosis stem pitting- associated virus (PBNSPaV), Little cherry virus 1 (LChV-1) and Little cherry virus 2 (LChV-2) was achieved with a quadruplex one-step RT-PCR, and a hybridization test with a multi-riboprobe, apparently representing the first successful attempt of concomitant identification of viruses of the family Closteroviridae in stone fruit trees. Quadruplex RT-PCR detected double (natural and artificial) and triple (artificial) infections in all samples tested, amplifying also a plant mRNA as an internal control. Standard and quadruplex RT-PCR were comparable in terms of detection limits and specificity. Molecular hybridization with a multi-riboprobe ('clotri') containing partial sequences of PBNSPaV, LChV-1 and LChV-2, allowed the detection of each of the three viruses in Prunus hosts. The sensitivity of single riboprobes and "clotri" was comparable.


Yesilcollou S.,Ege University | Minoia S.,CNR Institute of Plant virology | Torchetti E.M.,CNR Institute of Plant virology | Kaymak S.,Horticultural Research Institute of Egirdir | And 4 more authors.
Journal of Plant Pathology | Year: 2010

A survey of the presence of Pear blister canker viroid (PBCVd) in pear trees in Turkey was carried out by tissue print hybridization. Four out of 74 trees (5.4%) from a pear germplasm collection tested positive and this result was confirmed by RT-PCR and sequencing. Molecular characterization of the Turkish PBCVd isolates from four different local pear cultivars, together with multiple sequence alignments and phylogenetic analyses including all PBCVd sequences reported previously, provided an insight in the sequence variability of this viroid and showed that: (i) a total of 98 polymorphic positions distributed throughout the molecule are present in PBCVd genomic RNA; (ii) some regions with presumed relevant functions are strictly preserved and, (iii) the geographic origin of the isolates and the host species apparently have a bearing on the sequence variability of the viroid. This study records the presence of PBCVd in Turkey and confirms that its geographic distribution has been underestimated, most likely because the majority of infected pear cultivars are symptomless.


Wang L.P.,Huazhong Agricultural University | Wang L.P.,Agriculture and Agri Food Canada | Hong N.,Huazhong Agricultural University | Matic S.,CNR Institute of Plant virology | And 4 more authors.
Scientia Horticulturae | Year: 2011

A survey for apple and pear viruses was carried out at the Canadian Clonal Genebank (CCG), Harrow, Ontario, Canada, during the fall/winter of 2007 and spring of 2008. Leaves and/or dormant cuttings were randomly collected from 438 to 122 accessions of apple and pear, respectively. Samples were tested by Double Antibody Sandwich-Enzyme Linked Immunosorbent Assay (DAS-ELISA) for the presence of Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV) and Apple mosaic virus (ApMV). Infection rates for apples were ACLSV (48.1%), ASGV (10%), ASPV (6.6%) and ApMV (7.1%), and for pears ACLSV (42.6%). ACLSV was detected and characterization by multiplex RT-PCR with primers targeting a fragment of 677. bp corresponding to the partial coat protein (CP), movement protein (MP) and untranslated (3'UTR) region in 22 accessions of apple and pear. Multiplex RT-PCR showed a higher sensitivity over the ELISA test. The nucleotide and amino acid deduced partial CP identities ranged from 82.6-100% to 91-100%, respectively, while partial MP identities was 62.5-100% at aa level based on the amplified fragment appropriate for partial MP using a frame shift, among 22 ACLSV isolates. Phylogenetic analyses based on the partial CP region clustered CCG ACLSV isolates in two different groups, while those based on the partial MP region embraced CCG ACLSV isolates in two sub-clusters within the same group. This is the first report on the detection of ACLSV, ASPV, ASGV and ApMV at CCG, and the molecular characterization of ACLSV isolates in apple and pear plants from worldwide countries to deduce possible heterogeneity and evolution. © 2011 Elsevier B.V.

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