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Arosio P.,University of Milan | Arosio P.,Consortium for Science and Technology of Materials | Baldi G.,CERICOL | Chiellini F.,University of Pisa | And 20 more authors.
Dalton Transactions | Year: 2013

Encapsulated Fe3O4 nanoparticles of average diameters d = 12 nm are obtained by coprecipitation, in the presence of 2-methoxyethanol hemiester of poly(maleic anhydride-alt-butyl vinyl ether) 5% grafted with poly(ethylene glycol) (VP-MAG nanoparticles). A complete characterization of nude and encapsulated nanoparticles through structural techniques (namely XRD, TEM, SEM), Raman spectroscopy and magnetic measurements has been performed. These nanoparticles compared with commercial compounds (ENDOREM®) present superparamagnetic behavior and nuclear relaxivities that make them promising as magnetic resonance imaging (MRI) contrast agents (CAs). We found that our nanostructures exhibit r2 relaxivity higher than those of commercial CAs over the whole frequency range. The MRI efficiency of our samples was related to their microstructural and magnetic properties. © 2013 The Royal Society of Chemistry.


Psimadas D.,Technological Educational Institute of Athens | Psimadas D.,Institute of Nuclear and Radiological science and Technology | Baldi G.,CERICOL | Ravagli C.,CERICOL | And 5 more authors.
Nanotechnology | Year: 2014

Metal oxide nanoparticles, hybridized with various polymeric chemicals, represent a novel and breakthrough application in drug delivery, hyperthermia treatment and imaging techniques. Radiolabeling of these nanoformulations can result in new and attractive dual-imaging agents as well as provide accurate in vivo information on their biodistribution profile. In this paper a comparison study has been made between two of the most promising hybrid core-shell nanosystems, bearing either magnetite (Fe3O4) or cobalt ferrite (CoFe2O4) cores, regarding their magnetic, radiolabeling, hyperthermic and biodistribution properties. While hyperthermic properties were found to be affected by the metal-core type, the radiolabeling ability and the in vivo fate of the nanoformulations seem to depend critically on the size and the shell composition. © 2014 IOP Publishing Ltd.


Baldi G.,CERICOL | Bonacchi D.,CERICOL | Giudetti G.,European Commission - Joint Research Center Ispra | Lascialfari A.,University of Milan | And 11 more authors.
Small | Year: 2010

(Figure Presented) Chemical synthesis, stability, and characterization of a new albumin-based magnetic nanocarrier containing cobalt ferrite nanoparticles is reported. The BSA-cobalt-based nanocarrier is tested as a theranostic nanomedicine: both diagnostic abilities in vivo and therapeutic hyperthermic effects on standard human tumor cell line (HeLa cells) are investigated. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.


Horev-Azaria L.,Tel Aviv University | Baldi G.,CERICOL | Beno D.,Tel Aviv University | Bonacchi D.,CERICOL | And 14 more authors.
Particle and Fibre Toxicology | Year: 2013

Background: Cobalt-ferrite nanoparticles (Co-Fe NPs) are attractive for nanotechnology-based therapies. Thus, exploring their effect on viability of seven different cell lines representing different organs of the human body is highly important.Methods: The toxicological effects of Co-Fe NPs were studied by in-vitro exposure of A549 and NCIH441 cell-lines (lung), precision-cut lung slices from rat, HepG2 cell-line (liver), MDCK cell-line (kidney), Caco-2 TC7 cell-line (intestine), TK6 (lymphoblasts) and primary mouse dendritic-cells. Toxicity was examined following exposure to Co-Fe NPs in the concentration range of 0.05 -1.2 mM for 24 and 72 h, using Alamar blue, MTT and neutral red assays. Changes in oxidative stress were determined by a dichlorodihydrofluorescein diacetate based assay. Data analysis and predictive modeling of the obtained data sets were executed by employing methods of Knowledge Discovery from Data with emphasis on a decision tree model (J48).Results: Different dose-response curves of cell viability were obtained for each of the seven cell lines upon exposure to Co-Fe NPs. Increase of oxidative stress was induced by Co-Fe NPs and found to be dependent on the cell type. A high linear correlation (R2=0.97) was found between the toxicity of Co-Fe NPs and the extent of ROS generation following their exposure to Co-Fe NPs. The algorithm we applied to model the observed toxicity belongs to a type of supervised classifier. The decision tree model yielded the following order with decrease of the ranking parameter: NP concentrations (as the most influencing parameter), cell type (possessing the following hierarchy of cell sensitivity towards viability decrease: TK6 > Lung slices > NCIH441 > Caco-2 = MDCK > A549 > HepG2 = Dendritic) and time of exposure, where the highest-ranking parameter (NP concentration) provides the highest information gain with respect to toxicity. The validity of the chosen decision tree model J48 was established by yielding a higher accuracy than that of the well-known " naive bayes" classifier.Conclusions: The observed correlation between the oxidative stress, caused by the presence of the Co-Fe NPs, with the hierarchy of sensitivity of the different cell types towards toxicity, suggests that oxidative stress is one possible mechanism for the toxicity of Co-Fe NPs. © 2013 Horev-Azaria et al.; licensee BioMed Central Ltd.


Baldi G.,CERICOL | Ravagli C.,CERICOL | Mazzantini F.,CERICOL | Loudos G.,Technological Educational Institute of Athens | And 10 more authors.
International Journal of Nanomedicine | Year: 2014

Polymeric nanoparticles with targeting moieties containing magnetic nanoparticles as theranostic agents have considerable potential for the treatment of cancer. Here we report the chemical synthesis and characterization of a poly(D,L-lactide-co-glycolide)-b-poly(ethylene glycol)-based nanocarrier containing iron oxide nanoparticles and human epithelial growth factor receptor on the outer shell. The nanocarrier was also radiolabeled with 99mTc and tested as a theranostic nanomedicine, ie, it was investigated for both its diagnostic ability in vivo and its therapeutic hyperthermic effects in a standard A431 human tumor cell line. Following radiolabeling with 99mTc, the biodistribution and therapeutic hyperthermic effects of the nanosystem were studied noninvasively in vivo in tumor-bearing mice. A substantial decrease in tumor size correlated with an increase in both nanoparticle concentration and local temperature was achieved, confirming the possibility of using this multifunctional nanosystem as a therapeutic tool for epidermoid carcinoma. © 2014 Baldi et al.


Psimadas D.,University Hospital of Larissa | Psimadas D.,Institute of Radioisotopes Radiodiagnostic Products | Psimadas D.,Technological Educational Institute of Athens | Baldi G.,CERICOL | And 6 more authors.
Journal of Biomedical Nanotechnology | Year: 2012

Magnetic nanoparticles have become important tools for imaging a wide range of diseases, improving drug delivery and applying hyperthermic treatment. Iron oxide based nanoparticles have been widely examined, unlike cobalt ferrite based ones. Herein, monodisperse and stable CoFe 2O 4nanoparticles have been produced, coated and further stabilized using ethyl 12-(hydroxyamino)-12-oxododecanoate, poly(lactic-co-glycolic acid) and bovine serum albumin. The final product, NBRh1, was fully characterized and has been directly radiolabeled with 99mTc using SnCl 2 as the reducing agent in high yields. In vitro stability and hyperthermic properties of 99mTc-NBRh1 were encouraging for further application in low frequencies hyperthermia and biomagnetic applications. In vivo evaluation followed after injection in healthy mice. The planar and SPECT imaging data as well as the biodistribution results were in accordance, showing high liver and spleen uptake as expected starting almost immediately after administration. In conclusion the preliminary results for nanoparticles bearing a cobalt ferrite core justify further investigations towards potential hyperthermic applications, drug transportation and liver or spleen imaging. Copyright © 2012 American Scientific Publishers All rights reserved.


PubMed | Technological Educational Institute of Athens, CNR Institute of Chemistry of organometallic Compounds, University of Bologna, CERICOL and 2 more.
Type: | Journal: International journal of nanomedicine | Year: 2014

Polymeric nanoparticles with targeting moieties containing magnetic nanoparticles as theranostic agents have considerable potential for the treatment of cancer. Here we report the chemical synthesis and characterization of a poly(D,L-lactide-co-glycolide)-b-poly(ethylene glycol)-based nanocarrier containing iron oxide nanoparticles and human epithelial growth factor receptor on the outer shell. The nanocarrier was also radiolabeled with (99m)Tc and tested as a theranostic nanomedicine, ie, it was investigated for both its diagnostic ability in vivo and its therapeutic hyperthermic effects in a standard A431 human tumor cell line. Following radiolabeling with (99m)Tc, the biodistribution and therapeutic hyperthermic effects of the nanosystem were studied noninvasively in vivo in tumor-bearing mice. A substantial decrease in tumor size correlated with an increase in both nanoparticle concentration and local temperature was achieved, confirming the possibility of using this multifunctional nanosystem as a therapeutic tool for epidermoid carcinoma.


Blosi M.,CNR Institute of Science and Technology for Ceramics | Albonetti S.,University of Bologna | Gatti F.,University of Bologna | Dondi M.,CNR Institute of Science and Technology for Ceramics | And 4 more authors.
Nanotechnology 2010: Advanced Materials, CNTs, Particles, Films and Composites - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 | Year: 2010

A simple, microwave-assisted route for producing Au/Ag concentred sols by glucose reduction in water was developed. Ag-Au bimetallic nanoparticles stabilized by polyvinylpyrrolidone (PVP) were characterized and their catalytic activity was studied in the reduction of 4-nitrophenol (4-NP) with NaBH 4. Moreover they were tested as red ceramic inks for ink-jet printing technology. Since the research was developed in collaboration with a company interested in the large scale production of the suspensions, some fundamental properties for an industrial scale up were developed: high metal concentration, long time stability. The Au-Ag core-shell structures were prepared by a two-step process. Particle size-control and colloidal stability were achieved thanks to the accurate reaction optimization, combined with microwave heating, that allows the intensification of process even on large scale production. Prepared Au, Ag and Au/Ag nanocrystals acted as effective catalyst for the reduction of 4-NP.


Albonetti S.,University of Bologna | Blosi M.,CNR Institute of Science and Technology for Ceramics | Gatti F.,University of Bologna | Migliori A.,CNR Institute for Microelectronics and Microsystems | And 5 more authors.
Studies in Surface Science and Catalysis | Year: 2010

A simple, microwave-assisted, strategy for producing Au/Ag concentrated sols by glucose reduction in water was developed. Ag-Au bimetallic nanoparticles stabilized by polyvinylpyrrolidone (PVP) were characterized and their catalytic activity was studied in the reduction of 4-nitrophenol with NaBH4 as a probe reaction. The Ag-Au nanoparticles were prepared by first optimizing the synthesis of Au colloid and then carrying out the deposition of a silver shell. Microwave heating has been shown to provide more homogeneous particle nucleation and shorter synthesis time than traditional heating. Prepared Au, Ag and Au/Ag nanocrystals function as effective catalysts for the reduction of p-nitrophenol in the presence of NaBH4, otherwise unfeasible if only the strong reducing agent NaBH4 is employed. © 2010 Elsevier B.V. All rights reserved.

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