Osuna-Torres L.,Instituto Mexicano del Seguro Social |
Garcia-Marti X.,CIEF Vaersa Valencia |
Ventura-Zapata E.,CEPROBI IPN |
Zamilpa-Alvarez A.,Instituto Mexicano del Seguro Social |
And 3 more authors.
Forest Systems | Year: 2015
Aim of study: Taxus globosa is the only species of Taxus genus that grows in Mexico, and is a tree which is protected by the Mexican Government. This plant shares, with the European species T. baccata, the ability to produce taxanes, such as taxol. The objective of this work was to compare representative samples from two Taxus species: T. globosa Schltdl. (“Mexican yew”), and T. baccata L. (“European yew”), by employing different methodological chemical and pharmaco- logical tests. Area of study: Representative samples of Mexican yew were collected from states of Nuevo León, Querétaro, Hidalgo, and Oaxaca. The samples of European yew were taken from a clonal live collection located in the CIEF-Vaersa-Valencia (Spain). Materials and Methods: The aerial parts of all samples were extracted with methanol and subjected to TLC and HPLC analysis. Five extracts were selected for their anti-inflammatory evaluation. The extracts were tested on male ICR mice with ear inflammation induced with TPA, at a dose of 3.2 mg/ear. For T. globosa, an acute toxicity assay in female mice, according to OECD, was also conducted. Main results: Taxol was only detected in the yew trees samples collected in States of Hidalgo and Nuevo León (Mexico). In the inflammatory assay, in all treatments (except for that of MX35) induced slight, but significant, inhibition of inflammation, and only European sample M-9 showed inhibition of edema of >50%. T. globosa in the acute toxicity test causes only mild symptoms associated with a general depression of CNS, without being lethal. Research highlights: The aim of the present study was to perform a chemical analysis through quantification of taxol in the Mexican yew compared to European yew. In this research work was evaluated too for the first time the anti-inflammatory effect from both the Mexican and European yew. © 2015 INIA.
Guemes-Vera N.,National Autonomous University of Mexico |
Martinez-Herrera J.,CEPROBI IPN |
Hernandez-Chavez J.F.,Sonora Institute of Technology |
Yanez-Fernandez J.,UPIBI IPN |
Totosaus A.,Food Science Laboratory
Pakistan Journal of Nutrition | Year: 2012
Proximate composition, carotenoids, tannins, quinolizidine alkaloids and in vitro protein digestibility were determined in flours of two wild lupines seeds recollected at central region of Mexico. Varieties identified as Lupinus barkeri and Lupinus montanus were compared with a domesticated cultivated Lupinus albus crop. Although total protein content resulted significantly (p<0.05) higher for both L. barkeri and L. montanus, no significantly (p>0.05) difference were found in in vitro protein digestibility. Ash and crude fiber contents were significantly (p<0.05) higher for L. barkeri and L. montanus ether extract was significantly (p<0.05) higher than the other lupin samples. In general, chemical composition related to ash, fiber and ether extract contents are close to the reported range for other Lupinus species. Wild varieties of Lupinus could represent a viable alternative looking for new protein resources, from the techno-functional and nutritional point of view. Lupin flour is a good source of minerals and functional compounds, like carotenoids as antioxidant or dietary fiber, with health-promoting properties. Antinutritional factors associated to lupin can be minimized or eliminated by processing (soaking, dehulling and cooking). These characteristics of wild Lupinus varieties result in a revalorization of these crops as a protein and other healthy promoting compounds for human or animal consumption. © Asian Network for Scientific Information, 2012.
Rosas-Cardenas F.F.,CEPROBI IPN |
Paredes-Lopez O.,CEPROBI IPN |
Cruz-Hernandez A.,Laboratorio Nacional Of Genomica
Acta Horticulturae | Year: 2012
Fruit ripening involves a series of physiological, biochemical, and organoleptic changes that lead to the development of a soft and edible ripe fruit with desirable quality attributes. Due to its nutritional value and economic potential, and unique physiological characteristics, prickly pear is considered to be a model for the study of fruit ripening. In order to understand prickly pear fruit ripening, integration of physicochemical, biochemical and molecular studies are necessary. Knowledge of whole protein content will allow us to understand the different metabolic pathways involved in fruit ripening. In this work, we analyze protein differential expression and identify the proteins associated with ripening of prickly pears at different stages of development (early, intermediate and late ripening). Fruits from different morphospecies were harvested at green, middle-ripe and ripe stages. The proteins were extracted from the peel and resolved on 2-DE gel, analyzed with the Image master 2D Platinum 6.0 software (Amersham Biosciences) and grouped in Venn diagrams. Differential spots were analyzed by mass spectrometry approaches. There was differential expression and a high synthesis of proteins during ripening, the highest differential expression was obtained at the ripe stage of all materials. 1689 proteins were associated with ripening. We identified proteins associated with fruit ripening and with specific activities, i.e., photosynthesis, respiration, fatty acid and anthocyanins synthesis. This investigation provides the first proteomic approach for ripening of prickly pear fruits and allowed the analysis of the diversity of peptides and proteins that could be key to understanding and subsequent practical application for controlling ripening of this important fruit.
Peralta-Flores L.,CEPROBI IPN |
Gallegos-Tintore S.,CEPROBI IPN |
Solorza-Feria J.,CEPROBI IPN |
Chel-Guerrero L.,Autonomous University of Yucatan |
Martinez-Ayala A.,CEPROBI IPN
Grasas y Aceites | Year: 2012
J. curcas seed proteins were fractioned according to the Osborne method and some biochemical properties were determined for these fractions. Glutelins (378 g kg-1 protein) and globulins (201 g kg-1 protein) were the main components. Albumins and prolamins were the minor components. Protein digestibility was highest in glutelins and globulins with values of 81 and 80% respectively. Electrophoresis analysis showed that globulins and glutelins exhibited similar polypeptide profiles. Electrophoresis patterns suggested that there could be a structural relationship among 2S, 7S and 11S storage proteins from plant sources. According to the FAO?WHO reference, the protein fractions had acceptable levels of most of the essential amino acids, but its globulins and glutelins were low in lysine and tryptophan.
Gladiolus rust inoculation methods and evaluation of isothiocyanates of botanical extracts from plants of the brassicaceae family in rust control [Métodos de inoculación y evaluación de extractos botánicos e isotiocianatos de la familia brassicaceae en el control de la roya del gladiolo]
Ortega-Centeno S.,Autonomous University of the State of Morelos |
Guillen-Sanchez D.,Autonomous University of the State of Morelos |
Ramos-Garcia M.,CEPROBI IPN |
Troncoso-Rojas R.,CIAD |
And 4 more authors.
Revista Chapingo, Serie Horticultura | Year: 2010
Gladiolus rust caused by Uromyces transversalis (Thümen) G. Winter is listed as a major quarantine disease in Mexico. In Mexico, this fungus is controlled by continuous fungicide applications, which can result in fungus resistance, risk to human health and environmental pollution. The objectives of this research were to identify the most adequate method of artificial inoculation of U. transversalis on gladiolus plants under laboratory conditions and in vivo, to identify the main isothiocyanates of the extracts from plants of the Brassicacea family, and to determine their fungicidal potential together with the isothiocyanates of benzyl and phenyl to control this fungus under field and greenhouse conditions. Experiments were carried out in the regions of Yautepec and Ayala, Morelos, Mexico, and at the Center for Development of Biotic Products (CEPROBI) at the Instituto Politecnico Nacional. The main parameters evaluated in the field were disease severity, affected area, percentage infection and disease progress. In the greenhouse only percentage of infection and disease progress were studied. The results associated with artificial inoculation by the methods of spraying and steaming resulted in infection of 2 to 3 plants. The main isothiocyanates identified in the extracts applied were phenyl, benzyl, 2-phenylethyl, alyl and propyl. In the experiments carried out in Yautepec and Ayala, plants treated with the extract at the concentration of 0.1 % had a lower percentage infection than those treated at 0.2 % and 0.5%. Under greenhouse conditions, the isothiocyanates of phenyl and benzyl exerted good control of the fungus. In plants treated with extracts and isothiocyanates under greenhouse and field conditions no phytotoxicity was observed.