Silva A.M.M.,Faculdade Brasileira |
Ton A.,Centro Universitario Vila Velha |
Loureiro T.F.,Faculdade Brasileira |
Agrizzi B.L.,Faculdade Brasileira
Anais Brasileiros de Dermatologia | Year: 2011
Nicolau syndrome also known as Embolia cutis medicamentosa and Livedoid dermatitis is a rare complication characterized by tissue necrosis that occurs after injection of medicines. We describe a case of late development of Nicolau syndrome following intra-articular infiltration with corticosteroid. © 2011 by Anais Brasileiros de Dermatologia.
Negreiros L.A.,Centro Universitario Vila Velha |
Silva B.F.,Centro Universitario Vila Velha |
Paulino M.G.,Federal University of São Carlos |
Fernandes M.N.,Federal University of São Carlos |
Chippari-Gomes A.R.,Centro Universitario Vila Velha
Comparative Biochemistry and Physiology - C Toxicology and Pharmacology | Year: 2011
Hypoxia events are common in many aquatic systems, which may be a natural event or provoked by anthropogenic actions, as well as accidents involving oil occurring throughout the world are frequent. Thus, through the possibility of occurrence of these two situations in same place the purpose of this study was to evaluate if damage caused by crude oil on genotoxic and morphological parameters in the marine fish species Hippocampus reidi will be aggravated by events of severe hypoxia. Sea horses were exposed during 8 h to the following conditions: crude oil (OIL), severe hypoxia (HYP), association of severe hypoxia and crude oil (HYP + OIL) and normoxia without contaminant (CONT). An increase in micronuclei observed in OIL and HYP + OIL groups indicates that the crude oil exposure was a determining factor in the micronuclei induction and hypoxia did not intensify this result. In comet assays, both petroleum and hypoxia provoke DNA damage. The most frequent histopathology in the control groups and in those exposed to OIL and HYP + OIL groups were: hypertrophy and capillary dilation; hypertrophy and hyperplasia; hypertrophy, epithelial "lifting" and epithelial hyperplasia. An elongation of the lamellae was observed in fish from the two groups exposed to hypoxia, probably due to the fact that these groups required a greater flow of blood in the gills to increase the efficiency of gas exchange, since they were in a hypoxic environment. In summary, the micronuclei test and comet assay can be used as a good biomarker of contamination by petroleum. The association of hypoxia with crude oil in some aspects may exacerbate the responses of fish, in the light of the increase in DNA damage and the alterations in thickness of the gill epithelium. © 2011 Elsevier Inc.
Brinn R.P.,Florida International University |
Marcon J.L.,Federal University of Amazonas |
McComb D.M.,Florida Atlantic University |
Gomes L.C.,Centro Universitario Vila Velha |
And 2 more authors.
Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology | Year: 2012
Potamotrygon cf. histrix (cururu stingray) are endemic freshwater stingrays from the middle region of the Rio Negro in the Brazilian Amazon basin and are exported worldwide as ornamentals caught by artisanal fishermen. The transport process from capture to final destination is long and stressful. This study quantified stress related changes in corticosterone, blood and water samples (baseline, pre-transport, 3h, 12h and 24h) analyzed during a transport experiment which tested two water additives (tetracycline and the probiotic Efinol®). There was a significant stepwise increase in corticosterone levels in stingrays over transport time in combination with osmoregulatory disturbances suggesting a stress related role of this corticosteroid. There were significant increases in water conductivity, Na + and K + losses and ammonia excretion. Blood parameters such as glucose, hematocrit, red blood count and urea did not change significantly during the experiment. Glucose levels did not increase significantly during transport and this may be due to the fact that other elasmobranchs have been shown to rely more on ketone bodies for energy rather than glucose and produce ammonia as their main nitrogenous waste. The mineralocorticoid action of this hormone has been shown in elasmobranchs and most likely plays a role in osmotic homeostasis. The use of probiotic and especially antibiotic should be avoided since no beneficial effects were observed. © 2011 Elsevier Inc.
Schneider S.,Albert Ludwigs University of Freiburg |
Lenz D.,Centro Universitario Vila Velha |
Holzer M.,Albert Ludwigs University of Freiburg |
Palme K.,Albert Ludwigs University of Freiburg |
Suss R.,Albert Ludwigs University of Freiburg
Journal of Controlled Release | Year: 2010
Gene therapy is a promising therapeutic concept for a large number of incurable diseases. Lipid/DNA complexes (lipoplexes) are used to deliver genes into cells. However, while large efforts have been made to investigate the fate of lipoplexes once inside the cell, the rate of intracellular dissociation is still largely unknown. Analysis of the dissociation rates of DNA from lipid/DNA complexes is crucial for the evaluation of a gene delivery system's efficiency. This study introduces a new fluorescence resonance energy transfer (FRET) approach for the intracellular dissociation analysis of lipid/DNA complexes. Here, the labeling of both complex components, DNA as well as lipid, reveals whether DNA is still associated with the lipid or has dissociated. In this study the kinetic properties of complex dissociation were consistently measured with flow cytometry and fluorescence microscopy, and indicated that most complexes were dissociated after 24. h in A-10 cells. © 2010 Elsevier B.V.
Sarti T.D.,Federal University of Espirito Santo |
Campos E.A.C.,Federal University of Rio de Janeiro |
Zandonade E.,Federal University of Espirito Santo |
Enrico Cabral Ruschi G.,Centro Universitario Vila Velha |
Maciel E.L.N.,Federal University of Espirito Santo
Cadernos de Saude Publica | Year: 2012
Evaluation of health planning activities adapted to the local context is an essential part of daily work in the Family Health Strategy (FHS). This study aimed to evaluate the planning activities conducted by family health teams in Espírito Santo State, Brazil. This was an ecological study based on secondary data from 241 health teams in 46 municipalities (counties) in the State that use the Evaluation for Quality Improvement in the Family Health Strategy (AMQ-ESF) form. Criteria were set for evaluating the responses by health professionals to the quality standards in the AMQ-ESF forms, and health planning indicators were listed. The responses were stratified according to the municipality's population size and time since implementation of the FHS and population coverage. The study mostly showed low adequacy rates in the teams' work processes in relation to the AMQ quality standards, and stratification of the variables had no influence on the local planning practices. Specific measures are needed to upgrade the work processes in the FHS.
Ligeiro R.,Federal University of Minas Gerais |
Moretti M.S.,Federal University of Minas Gerais |
Moretti M.S.,Centro Universitario Vila Velha |
Goncalves Jr. J.F.,Federal University of Minas Gerais |
And 2 more authors.
Hydrobiologia | Year: 2010
The objective of this study was to evaluate the influences of detritus from the leaves of different species, and of exposure time on invertebrate colonization of leaves in a shaded Cerrado stream. We hypothesized that the exposure time is the main factor that influences the colonization of leaves by invertebrates. We used leaves of five tree species native to the Brazilian Cerrado: Protium heptaphyllum and Protium brasiliense (Burseraceae), Ocotea sp. (Lauraceae), Myrcia guyanensis (Myrtaceae), and Miconia chartacea (Melastomataceae), which are characterized by their toughness and low-nutritional quality. Litter bags, each containing leaves from one species, were placed in a headwater stream and removed after 7, 15, 30, 60, 90, and 120 days. The dominant taxon was Chironomidae, which comprised ca. 52% of all organisms and ca. 20% of the total biomass. The taxonomic richness of colonizing organisms did not vary among the leaf species. However, the density and biomass of the associated organisms varied differently among the kinds of detritus during the course of the incubation. The collector-gatherers and shredders reached higher densities in the detritus that decomposed more rapidly (Ocotea sp. and M. guyanensis), principally in the more advanced stages of colonization. The collector-filterers reached higher densities in the detritus that decomposed more slowly (P. heptaphyllum, P. brasiliense, and M. chartacea), principally in the initial stages of incubation. A cluster analysis divided the detritus samples of different leaf species according to the exposure time (initial phase: up to 7 days; intermediate phase: 7-30 days; advanced phase: 30-120 days), suggesting some succession in invertebrate colonization, with differences in taxon composition (indicator taxa analysis). These results suggest that regardless of the leaf-detritus species, exposure time was the main factor that influenced the colonization process of aquatic invertebrates. © 2010 Springer Science+Business Media B.V.
Santos C.A.,Centro Universitario Vila Velha |
Novaes L.S.,Centro Universitario Vila Velha |
Gomes L.C.,Centro Universitario Vila Velha
Zoologia | Year: 2010
The high toxicity of diesel components makes oil spills a threat to the biota in coastal marine environments. The genotoxic effect of the diesel water-soluble fraction (DWSF) on Hippocampus reidi (Ginsburg, 1933) was assessed. Fish were exposed to three different DWSF dilutions for up to 96 hours, and genotoxicity was analyzed using the micronuclei test and the comet assay. The micronuclei test revealed no significant differences between any of the DWSF dilutions and the control in the 24-hours period; however, micronuclei increased in fish exposed to 1:500 and 1:100 DWSF dilutions for 96 hours. For all dilutions, there was an increase in micronuclei in fish exposed for 96 hours when compared to those exposed for 24 hours. The tested dilutions increased frequencies of cell classes 2 (medium damage) and 3 (large damage) in the comet assay after 24 and 96 hours. Fish exposed to DWSF 1:100 exhibited a higher frequency of class 4 (apoptosis) cells in the 96-hours period. All dilutions increased the comet score when compared to the control at 24 and 96 hours. The micronuclei and comet tests were efficient in detecting DWSF genotoxic effects in H. reidi. © 2010 Sociedade Brasileira de Zoologia. All rights reserved.
Nesting ecology of Podocnemis expansa (Schweigger, 1812) and Podocnemis unifilis (Troschel, 1848) (Testudines, Podocnemididae) in the Javaés River, Brazil [Ecologia reprodutiva de Podocnemis expansa (Schweigger, 1812) e Podocnemis unifilis (Troschel, 1848) (Testudines, Podocnemididae) no Rio Javaés, Brasil]
Ferreira Jr. P.D.,Centro Universitario Vila Velha |
Castro P.T.A.,Federal University of Ouro Preto
Brazilian Journal of Biology | Year: 2010
Nest site has influence on incubation duration and hatching success of two Neotropical turtles, the Giant Amazon River Turtle (Podocnemis expansa) and Yellow-Spotted Side-Neck Turtle (Podocnemis unifilis - "Tracajá"). The 2000 and 2001 nesting seasons have been monitored at the Javaés River in Bananal Island, Brazil. Although they nest on the same beaches, there is a separation of the nesting areas of P unifilis and P. expansa nests on the upper parts of the beach. The incubation duration for P. expansa is influenced by the nesting period, the height of the nest from the river, the clutch size, and the grain size in the site of the nest. Nests of Podocnemis expansa placed in coarse sediments have shorter incubation duration than those placed in finer sediments. The hatching success in P. expansa is influenced by grain size, incubation duration, and nesting period. The grain size is negatively correlated with hatching success, indicating that the nests situated in finer-grained sand have better chances of successful egg hatching than those in coarser-grained sand. Nests of the end of the reproductive season have lower hatching success and incubation duration than those at the start of the season. For P. unifilis, the nesting period and nest depth influence the incubation duration; moreover, the river dynamics significantly affect the hatching success. The oscillation of the river level and the moment of initial increase, the height of the nest from the river level, and the nesting period are all decisive components for hatching success. The results of this research show the importance of protecting areas with great geological diversity, wherein the features of the environment can affect the microenvironment of nests, with consequences on incubation duration and hatching success.
Duca C.,University of Brasilia |
Duca C.,Centro Universitario Vila Velha |
Marini M.A.,University of Brasilia
Wilson Journal of Ornithology | Year: 2011
The Shrike-like Tanager (Neothraupis fasciata) is a Cerrado bird considered as near threatened. Its life history is poorly known, especially its reproduction. We monitored reproduction during four breeding seasons (2003-2006) with 120 nests in a protected area in central Brazil. Nesting began at the end of the dry season and start of the rainy season. The incubation (13.0 days) and nestling (11.7 days) periods were shorter than for most neotropical birds, but similar to some other tanagers. Clutch size (2-3 eggs) was similar to most tropical birds. However, clutch size increased and nest initiation date advanced ∼30 days in a year of early precipitation compared to 3 other years with regular or late precipitation. The Shrike-like Tanager had breeding flexibility and ability to adapt to changes in temporal precipitation patterns. © 2011 by the Wilson Ornithological Society.
Simoes L.N.,Centro Universitario Vila Velha |
Paiva G.,Centro Universitario Vila Velha |
de Carvalho Gomes L.,Centro Universitario Vila Velha
Pesquisa Agropecuaria Brasileira | Year: 2010
The objective of this study was to test the efficiency of clove oil as an anesthetic for adult tilapia (Oreochromis niloticus) and to evaluate, sensorially, the aroma and taste of fillets after anesthesia. In the first experiment, fish were exposed to different clove oil concentrations (0, 100, 150, 200, 250 and 350 mg L -1) of anesthetic baths. In the second experiment, different times of exposure (10, 20 and 30 minutes) to anesthesia were evaluated. Finally, the aroma and taste of the tilapia fillets, when exposed to optimal concentration of clove oil, were tested. The concentration of 250 mg L -1 of clove oil was adequate to induce lower opercular movement in adult tilapia, and for anesthesia for biometrics and brief management, the recommended concentration is 100 mg L -1. Fillets from tilapia anesthetized with clove oil showed a moderate difference in aroma and taste just after anesthesia. Thus, clove oil is an effective anesthetic for the management of adult tilapia as a routine procedure in fish farming, however tilapias should be slaughtered 12 hours after exposure to the drug, so as not to change the organoleptic characteristics of anesthetized fishes.