São José do Rio Preto, Brazil
São José do Rio Preto, Brazil

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Uscategui R.A.R.,São Paulo State University | Tiosso C.,São Paulo State University | Moro J.V.,São Paulo State University | Mostachio G.Q.,Centro Universitario Of Rio Preto | And 3 more authors.
Revista Colombiana de Ciencias Pecuarias | Year: 2017

Background: mastectomy and ovariohysterectomy (OVH) in bitches are critical surgeries and pain control can be challenging. Objective: to evaluate the efficacy of pre-emptive analgesia with methadone (MET) or tramadol (TRA) in postoperative pain intensity, cardiorespiratory effects, and anaesthetic/analgesic consumption in female dogs undergoing mastectomy and OVH. Methods: a prospective randomized blind clinical trial was used to evaluate 48 bitches of various breeds, aged 10 ± 3.7 years, weighing 16 ± 12 Kg, and with multiple mammary tumours. The animals were distributed in two groups: TRA group received 5 mg/Kg tramadol and MET group 0.5 mg/Kg methadone intramuscularly, 10 min prior to anaesthesia induction with propofol followed by maintenance with isoflurane. Heart (HR) and respiratory (RR) rates, mean arterial pressure (MAP), propofol induction dose (PID), oxyhemoglobin saturation (SpO2), end-tidal isoflurane concentration (EtISO), and carbon dioxide pressure (EtCO2) were measured during the intra-operative period. Post-operative pain was evaluated for 12 h and rated according to the Melbourne scale. Rescue analgesia (0.5 mg/Kg methadone, 2 mg/Kg lidocaine, or 0.01 mg/Kg/min ketamine IV) was given when necessary and post-operative analgesic consumption recorded. Statistical tests were used to compare treatments. Results: rescue analgesia requirements, pain score, PID and analgesic consumption were significantly lower (p<0.05) in MET group. The HR was higher in TRA group, while EtCO2 and MAP were higher in MET group (p<0.05). Conclusion: methadone was more effective than tramadol in pre-emptive analgesia but not completely adequate on controlling pain in bitches subjected to unilateral mastectomy and OVH. Methadone led to lower cardiovascular depression and lower propofol dose required for anesthesia induction. However, increased EtCO2 and special care with patient ventilation is advised. © 2017, Universidad de Antioquia. All rights reserved.

Da Cruz Furini A.A.,Centro Universitario Of Rio Preto | Schiesari A.,Jr. | Franco C.,Hospital Of Base | De Baptista A.R.S.,Federal University of Fluminense | Machado R.L.D.,Instituto Evandro Chagas
Revista da Sociedade Brasileira de Medicina Tropical | Year: 2015

Introduction: In this study, clinical-laboratory and epidemiological characteristics are described for a group of 700 individuals with HIV (human immunodeficiency virus)/AIDS (acquired immunodeficiency syndrome) in the ART (antiretroviral therapy) era at a teaching hospital that provides a quaternary level of care, with an emphasis on opportunistic infections (OIs), co-infections and immune profile. Methods: A retrospective cross-sectional study of AIDS cases was conducted from 1998 to 2008 by reviewing medical records from the Base Hospital/FUNFARME (Fundação Faculdade Regional de Medicina), São José do Rio Preto, São Paulo, Brazil. Results: The individuals were 14 to 75 years of age, and 458 were males. Heterosexuals accounted for 31.1% of all patients. Eighty-three percent were on ART, and 33.8% of those presented difficulties with treatment adherence. OIs were analyzed from medical records, and Pneumocystis jiroveci pneumonia was the most prevalent, regardless of the LTCD4+ (TCD4+ Lymphocytes) levels. Individuals whose viral loads were ≥10,000 showed a 90% greater chance of neurotoxoplasmosis. For P. jiroveci pneumonia, neurotoxoplasmosis, esophageal candidiasis, pulmonary tuberculosis and neurocryptococcosis, the chances of infection were higher among patients with LTCD4+ levels below 200 cells/mm3. HIV/ hepatitis C virus (HCV) and HIV/hepatitis B virus (HBV) co-infections were significantly associated with death. Conclusions: OIs remain frequent in the ART era even in populations where the access to medical care is considered satisfactory. © 2015, Sociedade Brasileira de Medicina Tropical. All rights reserved.

Davanso T.M.,São Paulo State University | Taddei F.G.,Centro Universitario Of Rio Preto | Simoes S.M.,São Paulo State University | Fransozo A.,São Paulo State University | Da Costa R.C.,São Paulo State University
Journal of Crustacean Biology | Year: 2013

In the northwestern region of the state of São Paulo, Brazil, the freshwater crab Dilocarcinus pagei Stimpson, 1861 is usually sold as bait for several fish species for sport fishing. Thus, knowledge of the reproductive biology of this species is essential to provide information for maintaining natural stocks and implementing future farming initiatives. The objective of this study was to investigate the population dynamics of the red freshwater crab D. pagei, focusing on reproductive and juvenile recruitment periods, sex ratio, and individual growth. Sampling was performed monthly from October 2005 to September 2007 in the Marimbondo-Fumas Hydroelectric Plant reservoir, Rio Grande, municipality of Icém in northwestern São Paulo state. The individuals were sexed, carapace widths were measured, and the gonads were examined macroscopically. In total, 1002 individuals were analyzed, consisting of 568 males and 434 females, of which 4 were ovigerous and 35 carried early juveniles in the incubatory chamber. The largest numbers of reproductive individuals of both sexes were recorded during the winter and spring months before spawning, which occurred in the spring. Intense juvenile recruitment and the highest rainfall levels were observed during the Southern Hemisphere summer. The results indicate that spawning can be considered seasonal, and the simultaneous emergence of juveniles with high rainfall levels is probably the result of the higher availability of food and potential sites for protection against predators. The skewed sex ratio (♂ 1:0.76 ♀) may be associated with growth rates, mortality, food restriction, behavioral differences, migration, and differential habitat use between the sexes. The similar maximum size (CW∞) and growth constant (k) for males (k = 1.78 y-1, CW = 61.43 mm) and females (k = 1.67 y-1, CW = 60.66 mm) may be associated with different energy investment strategies between the sexes. Additionally, a closed season is proposed for the species. © The Crustacean Society, 2013. Published by Brill NV, Leiden.

Galvao A.L.B.,Centro Universitario Of Rio Preto | Carvalho M.B.,São Paulo State University
Semina:Ciencias Agrarias | Year: 2014

Nitric oxide (NO) is a free radical gas, inorganic, which has seven electrons of nitrogen and oxygen eight, possessing an unpaired electron. This radical is produced from L-arginine by a reaction mediated by the enzyme NO synthase. NO it is about a radical of who acts abundant on a variety of biological processes, particularly when produced by endothelial cells plays a significant role in cardiovascular control, as a modulator of peripheral vascular resistance and platelet aggregation. This free radical has also a neurotransmitter and mediator of the immune system. NO kidney function has been considered in many physiological functions such as: (a) regulation of hemodynamics and glomerular function tubuloglomerular, (b) participation in pressure natriuresis (c) maintaining medullar perfusion (d) inhibiting sodium reabsorption tubular, and (e) acting as a modulator of the activity of the sympathetic nervous system. Given these functions, the occurrence of its deficiency is associated with chronic kidney disease (CKD) in vasoconstriction and consequently glomerular hypertension, high blood pressure (HBP), proteinuria and progression of renal dysfunction. This work has the scope to describe the role of NO in renal physiology and pathophysiology of CKD.

Guerra A.L.,São Paulo State University | Junior Lucato R.V.,São Paulo State University | Chiachio M.C.,Centro Universitario Of Rio Preto | Taddei F.G.,Centro Universitario Of Rio Preto | Castiglioni L.,São Paulo State University
Genetics and Molecular Research | Year: 2014

The genus Macrobrachium includes prawns, which are widely distributed in lakes, floodplains, and rivers in tropical and subtropical regions of South America. This genus presents nearly 210 known species with great ecological and economic importance. However, few studies are related to the biology of these crustaceans. In this study, we analyzed the genetic variability and phylogenetic relationship between Macrobrachium amazonicum and Macrobrachium jelskii, which are closely related species. Additionally, they are syntopics and their taxonomy poses problems because it is difficult to differentiate between the species. We used the mitochondrial gene sequences COI and 16S rRNA to assess the genetic structure of these species in 3 populations that were collected from Tiete hydrographic basin (São Paulo State, Brazil). The interspecific comparison of samples that were collected at the same and different locations showed a low rate of genetic variability. This similarity was attributed to the recent introduction of these species in the regions that were sampled and the habitat conditions in which they inhabit. In addition, these results may be consistent with the hypothesis that they are a single species, interspecific hybrids, or metapopulation. The dendrogram analyses did not reveal the formation of clusters, confirming the disturbances in the genetic structure of the samples that were analyzed in this study. These data are pioneers to these crustaceans, and they confirm the ecological and evolutionary problems between these Macrobrachium species. © FUNPEC-RP.

This study aimed to evaluate growth rates of Dilocarcinus pagei Stimpson, 1861 from Barra Mansa dam, Mendonça, SP (21° 14' 27"S e 49° 56' 28"W), provide information on maximum size attained, longevity and growth rates for males and females, and then relate these data with reproductive biology of the species. For this, the carapace width (CW) of 1340 specimens of D. pagei, 804 males and 536 females, were measured over the period between july/2006 to june/2007, and their individual growth was determined by CW size classes distribution method. Data was grouped in three-months periods and then analyzed with the FISAT software. The normal components were decomposed following the Bhattacharya method and confirmed by NORMSEP routin, which provided the means and respective standard deviations. Growth rates over the three-months periods and the age cohorts were obtained after the means. The obtained curves for males and females, respectively, were LC = 55,36[1-e-0,68 (t+0,056871)], LC = 63,65[1-e-0,73(t+0,045956)]. Females showed growth rate and maximum size larger than males, probably because the larger size favors the gonads development, resulting in larger spawning in a short period of time. Age cohorts analyses indicate seasonal reproduction, with recruitment pulse in spring. These results indicate that these crabs growth rate is related to their reproductive strategy, possibly the main onthogenetic development selective pressure. Age cohorts analyses indicate seasonal reproduction, with recruitment pulse in spring. These results indicate that these crabs growth rate is related to their reproductive strategy, possibly the main onthogenetic development selective pressure.

Canada M.L.M.,Centro Universitario Of Rio Preto | Canada M.L.M.,University Camilo Castelo Branco | Abelan U.S.,Centro Universitario Of Rio Preto | Zangaro R.A.,University Camilo Castelo Branco | And 2 more authors.
Revista Brasileira de Engenharia Biomedica | Year: 2014

Introduction: Ozone is a potent antibacterial agent. Because ozone oxidizes organic material, it directly attacks microorganisms resulting in safe, fast and economical sterilization at low temperatures. This study evaluated the efficacy of ozonated water in the reprocessing of dialyzers obtained from a hemodialysis service in São José do Rio Preto. Methods: A total of 23 blood dialyzers were collected at the conclusion of the morning hemodialysis shift. The equipment was collected after the maximum number of reuses, with the last disinfection performed with purified water (obtained by reverse osmosis and subsequent reverse ultrafiltration). The number and species of microorganisms in the dialyzers were evaluated before and after treatment with ozonated water. The ozonation of sterile deionized water was achieved by direct contact between the water and the ozone generating equipment. Results: The mean number of microorganisms before sterilization was 1,47.109 colony forming units per ml (CFU/ml). After treatment with ozonated water, the number of microorganisms was 4,80.101 CFU/ml. Conclusion: Ozonated water is an effective decontaminant for most blood dialyzers. © 2014 Sociedade Brasileira de Engenharia Biomedica. All rights reserved.

Gomes A.F.,Fundacao Educacional de Barretos | Gomes A.F.,Centro Universitario Of Rio Preto | Vidiella-Barranco A.,University of Campinas
Applied Mathematics and Information Sciences | Year: 2014

We investigate the transfer of coherence from atoms to a cavity field initially in a statistical mixture within a two-photon micromaser arrangement. The field is progressively modified from a maximum entropy state (thermal state) towards an almost pure state (entropy close to zero) due to its interaction with atoms sent across the cavity. We trace over the atomic variables, i.e., the atomic states are not measured and recorded by a detector after they leave the cavity. We show that by applying an external classical driving field it is possible to substantially increase the field purity without the need of previously preparing the atoms in a superposition of their energy eigenstates. We also discuss some of the nonclassical statistical properties of the resulting field. © 2014 NSP Natural Sciences Publishing Cor.

Bonine B.M.,Paulista University | Polizelli P.P.,Centro Universitario Of Rio Preto | Bonilla-Rodriguez G.O.,Paulista University
Enzyme Research | Year: 2014

This study reports the immobilization of a new lipase isolated from oleaginous seeds of Pachira aquatica, using beads of calcium alginate (Alg) and poly(vinyl alcohol) (PVA). We evaluated the morphology, number of cycles of reuse, optimum temperature, and temperature stability of both immobilization methods compared to the free enzyme. The immobilized enzymes were more stable than the free enzyme, keeping 60% of the original activity after 4 h at 50°C. The immobilized lipase was reused several times, with activity decreasing to approximately 50% after 5 cycles. Both the free and immobilized enzymes were found to be optimally active between 30 and 40°C. © 2014 Bárbara M. Bonine et al.

Fernandes C.,São Paulo State University | Da Silva R.P.,São Paulo State University | Marcelo A.V.,Centro Universitario Of Rio Preto
Revista Brasileira de Ciencia do Solo | Year: 2015

Soil quality indicators such as penetration resistance (PR) and bulk density (BD) are traditionally determined in a single undisturbed soil sample. The aim of this study was to assess the effect of PR measurements of undisturbed samples on the determination of BD in the same sample of two soils differing in clay contents. To this end, samples were collected from the 0.00-0.10 and 0.10-0.20 m layers of two soils of clayey and very clayey texture. Volumetric rings were used to collect a total of 120 undisturbed soil samples from each soil layer that were divided into two subsets containing 60 units each. One sample set, designated “perforated samples”, was used to determine PR and BD in the same undisturbed sample; the other, named “intact samples”, was used to determine BD only. Bulk density values for perforated and intact samples were compared by analysis of variance, using a completely randomized experimental design. Means were compared by the t-test at 5 %. The BD values for the clayey soil were similar in perforated and intact samples from the two layers. However, BD of the very clayey soil was lower in the perforated than in the intact samples at both depths. Therefore, PR and BD in clayey soils can be accurately determined in the same undisturbed sample whereas in very clayey soils, different samples are required for this purpose. © 2015, Revista Brasileira de Ciencia do Solo. All rights reserved.

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