Centro Universitario Of Lavras

Lavras, Brazil

Centro Universitario Of Lavras

Lavras, Brazil
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The β-blocker drug atenolol was subjected to forced degradation testing in this study, to assess the specificity of the analytical method used. The degradation conditions employed were: light, heat, humidity, acid/ base hydrolysis, oxidation and exposure to metal ions. The analytical method was that described in the 5th edition of Farmacopeia Brasileira to determine the drug content of atenolol tablets. Breakdown products were detected by means of reversed-phase HPLC (column: C18, 250 mm x 4.6 mm, 5 μm; mobile phase: aqueous solution of 1.1g sodium 1-heptanesulfonate and 0.71g anhydrous disodium phosphate (phosphoric acid to pH 3.0): methanol: dibutylamine 70:30:2, flowing at 0.6 mL/min), with UV-DAD at 226nm. Seven degradation products were observed; none of these showed the same retention time as the atenolol and there were no overlapping peaks. The analytical method was thus demonstrated to be specific, indicative of stability and validated for the content determination of atenolol tablets. © 2014, Universidade Estadual Paulista (UNESP). All rights reserved.


Alves D.A.G.,University of Sao Paulo | Alves D.A.G.,Centro Universitario Of Lavras | Carvalho L.B.C.D.,University of Sao Paulo | Morais J.F.D.,University of Sao Paulo | Prado G.F.D.,University of Sao Paulo
Sleep Medicine | Year: 2010

Objective: The objective of this study was to identify the prevalence of restless legs syndrome (RLS) among pregnant Brazilian women, with individual diagnostic clinical interviews during the trimesters of pregnancy, and to determine the severity. Methods: It was a cross-sectional study. We interviewed 524 pregnant women (18-45. years old) who came to the prenatal outpatient clinic to consult an obstetrician. We used a RLS clinical-diagnostic interview and the International RLS Study Group rating scale (IRLS). Results: The prevalence of RLS during pregnancy was 13.5% in our sample, among which 90.1% of the cases started with their symptoms during pregnancy. More than half of the patients (53.5%) presented severe or very severe symptoms and the largest proportion of them (15.2%) were in their third trimester. We did not observe any demographic differences among the trimesters for RLS prevalence and RLS severity. Conclusion: RLS during pregnancy is more frequent than in the general population, such that more than half of the pregnant women with RLS present it severely or very severely. It occurs especially in the third trimester. © 2010 Elsevier B.V.


de Abreu P.,Setor de Meio Ambiente | Pereira E.L.,University of Sao Paulo | Campos C.M.M.,Federal University of Lavras | Naves F.L.,Centro Universitario Of Lavras
Acta Scientiarum - Technology | Year: 2013

Advanced Oxidation Processes (AOP) have attracted much interest of scientific and industrial communities. Therefore, this process was employed experimentally to determine its efficacy in treating dairy effluents. For this, it was employed an artificial light reactor using photolysis (UV), hydrogen peroxide (H2O2) as an oxidant, and zinc oxide (ZnO) as an alternative catalyst. The variables studied in the photocatalysis were: H2O2, ZnO, under different values of pH, both in relation to the interferences with the reaction rate and with the process efficiency. First order differential equations were applied in determining the degradation rate over the hydraulic retention time (HRT); the F-test and the signal: noise ratio comparison were used to test the significance of variables. The AOP using photolysis UV, H2O2 and ZnO was effective in COD removal with 80% efficiency. As for the sterilization, in terms of total and fecal coliforms the efficiency was 100%, with the most probable number of total and fecal coliforms (MPN 100 mL-1) equal to zero. A catalyst/oxidant/pH ratio was observed at 1 g 30 mL-1 4-1 for a better kinetic-chemical reaction, for 1 hour lighting.


Henrique P.C.,Federal University of Lavras | Alves J.D.,Federal University of Lavras | Deuner S.,Embrapa Cerrados | Goulart P.F.P.,Centro Universitario Of Lavras | do Livramento D.E.,Federal University of Lavras
Pesquisa Agropecuaria Brasileira | Year: 2011

The objective of this work was to evaluate the physiological aspects of the development of coffee seedlings grown under colored screens with different spectral characteristics. Seedlings of Catucaí Amarelo 2SL, in the stage known as "orelha de onça", were arranged in a randomized block design, with five replicates, under structures individually covered with blue, white, gray, black or red screens with 50% shade. Four months after, evaluations were done for seedling growth, pigment content of the leaves, total soluble sugars and starch contents of the leaves and roots. The red screen was the most effective in promoting growth in four out of the seven studied traits: plant height, leaf area and leaf dry weight and total dry matter. For the other characteristics, there was no difference among the screens. The pigment analysis showed that, except for the gray screen, the other ones did not differ for this trait. In leaves, the red screen promoted higher levels of carbohydrates and starch. At the root, carbohydrate contents were higher under the red and black screens. Among the five screen colors, the red one was the most efficient in the production of coffee seedlings with higher vigor and quality, with outstanding carbohydrate contents and biomass.


Souza-Silva M.,Centro Universitario Of Lavras | Ferreira de Oliveira Bernardi L.,CAPES | Ferreira de Oliveira Bernardi L.,Federal University of Lavras | Parentoni Martins R.,Federal University of Ceará | Lopes Ferreira R.,Federal University of Lavras
Acta Carsologica | Year: 2012

Caves are permanently aphotic environments, a fact that precludes the occurrence of photosynthetic organisms. In these systems the resource is allochthonous, coming mainly from the surrounding epigean environment, being imported by physical and biological agents. Even knowing about the importance of the organic allochthonous resources in caves, little is known of their importation and processing. The present work had as an objective, the measuring the coarse particulate organic matter processing and import rates in the subterranean environment. The cave studied was Lapa da Fazenda Extrema I, limestone cave, located in Brazilian savanna biome. Through bimonthly collections, it was observed that the organic detritus penetrated into the cave in low amounts in dry season and high amounts in rainy season. The processing of the organic plant matter in the aquatic hypogean environment was moderate (K-day=0.025), in the epigean environment the processing was predominantly slow (K-day =0.0104). The detritus commonly brought to the interior of the cave were large woods (58.18 g/day), followed by leaves and fragmented material (12.76 g/day), fruits and seeds (0.0069 g/day), animal carcasses (0.002 g/day) and roots (0.001 g/day). The highest richness and abundances of invertebrates were found in the same periods in which there were the highest rates of organic matter import to the cave.


da Silva A.C.,Federal University of Lavras | de Souza P.E.,Federal University of Lavras | Machado J.C.,Federal University of Lavras | da Silva B.M.,Centro Universitario Of Lavras | Pinto J.E.B.P.,Federal University of Lavras
Tropical Plant Pathology | Year: 2012

The aim of the study was to evaluate the antifungal activity of essential oils from "hortelã do campo" (Hyptis marrubioides), "alfazema-do-Brasil" (Aloysia gratissima) and "erva-baleeira" (Cordia verbenacea) and their effcacy in the treatment of soybean seeds infected with Colletotrichum truncatum. In vitro assays were performed to evaluate the effects of the oils on spore germination, mycelial growth, and the production and viability of C. truncatum conidia. Soybean seeds inoculated with C. truncatum were treated with essential oils at concentrations ranging from 0.5 to 2% and grown under greenhouse conditions. Seed health and germination percentages were evaluated according to standard protocols. Seedling emergence, stand establishment and the percentages of dead seedlings were determined, together with the emergence speed index, plantlet height and dry weight of aerial biomass. The oils from H. marrubioides, C. verbenacea and A. gratissima inhibited the germination and production of conidia as well as the growth of C. truncatum. At concentrations ≥ 1%, the effectiveness of the oils against soybean anthracnose was superior to, or at least comparable with, that of the fungicide carbendazim. The viability of conidia and the germination of soybean seeds were not affected by any of the treatments with essential oils. It is concluded that essential oils from H. marrubioides, A. gratissima and C. verbenacea have potential as alternatives to synthetic fungicides in the control of anthracnose in soybean seeds. © Brazilian Phytopathological Society.


The composition of Pimplinae taxocenosis (Hymenoptera, Ichneumonidae) was investigated in the Reserva Biológica Unilavras/Boqueirão, Ingaí, Minas Gerais. Samples were collected using two Malaise traps (Townes model), installed in a gallery forest and a savanna area between April 2005 and February 2006, totalizing 16 samples in each environment. One hundred specimens of Pimplinae were collected, being represented by 26 species and distributed in 12 genera. Pimpla and Neotheronia were the most abundant with 65 collected specimens. In gallery forest 71 individuals were collected and in savanna, 29. This survey expands the distributional records of Eruga draperi Gauld, 1991; Polysphincta thoracica (Cresson, 1874); Zaglyptus simonis (Marshall, 1892), and Neotheronia hespenheidei Gauld, 1991 towards the Cerrado domain.


Magalhaes K.T.,Federal University of Lavras | de Pereira G.V.M.,Federal University of Lavras | Dias D.R.,Centro Universitario Of Lavras | Schwan R.F.,Federal University of Lavras
World Journal of Microbiology and Biotechnology | Year: 2010

The microorganisms associated with sugary Brazilian kefir beverage were investigated using a combination of culture-dependent and -independent methods. A total of 289 bacteria and 129 yeasts were identified via phenotypic and genotypic methods. Lb. paracasei (23.8%) was the major bacterial isolate identified, followed by Acetobacterlovaniensis (16.31%), Lactobacillus parabuchneri (11.71%), Lactobacillus kefir (10.03%) and Lactococcus lactis (10.03%). Saccharomyces cerevisiae (54.26%) and Kluyveromyces lactis (20.15%) were the most common yeast species isolated. Scanning electron microscopy showed that the microbiota was dominated by lemon-shaped yeast cells growing in close association with Lactobacillus (long and curved). Some lactic acid bacteria detected by sequence analysis of DGGE (denaturing gradient gel electrophoresis) bands were not recovered at any time through fermentation by plating. Conversely, DGGE fingerprints did not reveal bands corresponding to some of the species isolated by culturing methods. The bacteria Acetobacter lovaniensis and the yeast Kazachstania aerobia are described for the first time in sugary kefir. During the 24h of fermentation, the concentration of lactic acid ranged from 0.2 to 1.80mg/ml, and that of acetic acid increased from 0.08 to 1.12mg/ml. The production of ethanol was limited, reaching a final mean value of 1.24mg/ml. © Springer Science+Business Media B.V. 2010.


Alves J.A.,Federal University of Lavras | Lima L.C.D.O.,Federal University of Lavras | Dias D.R.,Centro Universitario Of Lavras | Nunes C.A.,Federal University of Lavras | Schwan R.F.,Federal University of Lavras
International Journal of Food Science and Technology | Year: 2010

The aim of this study was to evaluate the contribution of yeast to the volatile profile of beverages obtained by the fermentation of lychee must through inoculated (Saccharomyces cerevisiae strains UFLA CA116, UFLA CA1183 and UFLA CA1174) and spontaneous fermentation. Higher alcohols and esters were the primary volatiles detected by SPME/GC-MS. A Principal Component Analysis indicated similarities between UFLA CA116 and UFLA CA1183 and between UFLA CA1174 and spontaneous fermentation. Changing the yeast strain used in the fermentation process has the potential to modulate the volatile profile of fermented beverages. The beverage produced by the inoculation of yeast CA1183 showed the most complex aroma profile compared to the other beverages. © 2010 The Authors. International Journal of Food Science and Technology © 2010 Institute of Food Science and Technology.


Objective. The purpose of this paper was to evaluate quality and quantity of sleep of chronic renal patients undergoing hemodialysis in Santa Casa da Misericórdia Hospital in Lavras - Minas Gerais, Brazil. Method. Forty patients between 40 and 60 years old took part in the study. The Diary of Sleep was used to evaluate the quantity and The Pittsburgh Sleep Quality Index to evaluate the quality of sleep in those patients. Results. According to the descriptive analysis of The Diary of Sleep, the average of the total time of sleep was 6.2h (±1,3), the average of the number of n aps taken per day was 0.4 (±0,5) and the night awakenings had the average of 0.9 (±1,0). The analysis of The Pittsburgh Sleep Quality Index showed that 20% of the patients displayed good quality of sleep, 52.5% displayed bad quality of sleep and 27.5% displayed sleep disorders. Conclusion. The conclusion that follows from this study is that the majority of patients presented, according to The Diary of Sleep, less than satisfactory sleep quantity and according to The Pittsburgh Sleep Quality Index, bad sleep quality or sleep disorders.

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