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Zavala-Aguirre J.L.,Autonomous University of Guadalajara | Torres-Bugarin O.,Autonomous University of Guadalajara | Flores-Kehn L.P.,Autonomous University of Guadalajara | Ramos-Ibarra M.L.,Centro Universitario Of Ciencias Biologicas ropecuarias | And 2 more authors.
Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering | Year: 2010

This study is a follow-up of previous research in which we described the frequency of spontaneous micronucleated erythrocytes (MNE) in the Goodeid Xenotocoa melanosoma collected from Lake La Alberca, located in the state of Michoacan, México. In the present work, we measured micronuclei (MN) and nuclear abnormalities (NA) in erythrocytes of peripheral blood. Bioassays taken at 24 or 96 hours in either the cyclophosfamide (CP) or colchicine (COL) showed a significant increase in MN and BC (P values ranging from 0.0499 to 0.0036) compared with information from wild organisms collected over 3 years. Concentrationdependent and time-dependent responses support the proposal of using endemic Xenotoca melanosoma as a bioindicator of genotoxicity and cytotoxicity with a high transcendence for the health of the entire ecosystem and evaluation of the Lerma-Chapala watershed.

Villasenor-Cabrera T.,Hospital Civil Fray Antonio Alcalde | Pacheco-Sanchez I.,Centro Universitario Of Tonala | Nuno-Romero R.,Centro Universitario Of Ciencias Biologicas ropecuarias | Garcia-Estrada J.,University of Guadalajara
Revista de Neurologia | Year: 2016

Introduction. Gastroschisis is a malformation of the abdominal wall that is corrected by surgery and requires special hospital care, such as immobilisation, dietary restriction and separation from the mother for a variable period of time, among others. To analyse the possible cognitive repercussions, the Bayley III test was administered to 14 children. Subjects and methods. The study was conducted with seven cases (born with gastroschisis) and seven controls, in civil hospitals in Guadalajara, over the period January-April 2013. Results. No evidence of retardation was found in the cases in the cognition, language and motor conduct assessments, whereas in those associated with socio-emotional and adaptive behaviour the scores favoured the control group. This shows that the latter have better adaptation and interaction strategies with respect to the cases group. Conclusions. Gastroschisis will not affect later neurodevelopment, at least in this group which presented only this abnormality and deficits that can be explained by their perinatal circumstances. Nevertheless, this prognosis cannot be generalised to other cases with a more severe clinical picture, a longer stay in hospital and associated comorbidity. In any case, it is important to inform the parents as of the prenatal stage about the expected consequences of this pathology. © 2016, Revista de Neurologia. All rights reserved.

Canales-Aguirre A.A.,Research Center y Asistencia en Tecnologia | Gomez-Pinedo U.A.,Institute Neurociencias | Luquin S.,University of Guadalajara | Ramirez-Herrera M.A.,Laboratorio Of Neurofisiologia | And 2 more authors.
Nutritional Neuroscience | Year: 2012

One of the main concerns regarding organophosphate pesticides (OP) is their possible toxic effects. Doses that do not produce acute toxicity are capable of altering the structure and biochemistry of different tissues and organs by production of reactive oxygen species (ROS). Curcumin (CUR) is the main substance in Curcuma longa (Zingiberacea) rhizome that has strong antioxidant activity. However, the neuroprotective properties of curcumin against oxidative stress induced by prolonged exposure to parathion (PAR) is not clear. Objective: The present work evaluated the protective effect of curcumin against the oxidative damage induced in the rat hippocampus by the OP PAR. Methods: Forty female Wistar rats were distributed in four groups as follows: exposed to PAR by inhalation (PAR group); pre-treated with CUR and then exposed to PAR by inhalation, (CUR + PAR group); exposed to environmental air and treated with CUR in the food (CUR group); and exposed to environmental air (the control group). At the end of the handling process, the concentration of erythrocyte cholinesterase was monitored, as indicator of PAR intoxication and lipoperoxidation, immunohistochemistry for astrocytes, and activated microglia and apoptosis was determined in the hippocampus. Results: In the present study, we show that the administration of CUR (200 mg/kg body weight) significantly diminished the oxidative damage in the hippocampus of rats exposed to the OP PAR. Discussion: These data suggest that CUR may be an alternative to prevent neurodegenerative damage after pesticide exposure. © W.S. Maney & Son Ltd 2012.

Reyes-Velasco J.,University of Texas at Arlington | Reyes-Velasco J.,Simon Bolivar University of Venezuela | Ahumada-Carrillo I.,Centro Universitario Of Ciencias Biologicas ropecuarias | Burkhardt T.R.,10150 West Rudasill Rd. | Devitt T.J.,University of Texas at Austin
Zootaxa | Year: 2015

We describe two new species of Eleutherodactylus, subgenus Syrrhophus, from two separate mountain ranges in western Mexico. Eleutherodactylus grunwaldi sp. nov. inhabits the Sierra de Manantlán in Colima and Jalisco from 1300 to 2200 m, whereas E. wixarika sp. nov. is known from a single locality in the Sierra Huichola of northern Jalisco at 2400 m, but is probably more widespread. Eleutherodactylus grunwaldi is readily distinguishable from most members of mainland Syrrhophus by a combination of its large size, broad, truncate digital pads more than three times the narrowest part of the digit, and a black and green marbled color pattern. This species is saxicolous, inhabiting limestone outcrops, and has been found in caves during the dry season. Eleutherodactylus wixarika is a moderate sized species, most similar to E. teretistes, E. pallidus and E. modestus. It is distinguished from all other members of the subgenus by the combination a tuberculate, reddish dorsum, lack of compact lumbar glands, and expanded digital pads less than twice the width of the narrowest part of the digit. This species inhabits areas with secondary vegetation in pine forest. Males of both species call at night during the rainy season. The advertisement call of both species consists of a short, narrow band, pure-tone note organized into a discrete train at a rate of about six times per minute. Spectral and temporal acoustic properties differ between species. The subgenus Syrrhophus of the genus Eleutherodactylus is one of the most poorly studied groups of frogs in Mexico but probably one of the most diverse. Copyright © 2015 Magnolia Press.

Jay O.,University of Salvador | Torres V.,Institute Ciencia Animal | Marrero Y.,Institute Ciencia Animal | Torres P.,Centro Universitario Of Ciencias Biologicas ropecuarias
Cuban Journal of Agricultural Science | Year: 2012

In order to find a tool for comparing multiple curves of in vitro gas production to assess ruminants' feeds, the square mean distance between curves is proposed as statistical distance. The performance of four comparison tests of fixed range using this distance: lowest significant distance (LSD), Tukey's honest significant distance (HSD), Scheffé's significant distance (SSD) and Bonferroni-corrected significant distance (BSD) through four experiments simulated by Monte Carlo. The signification level of the tests is also analyzed when the number of treatments or the variation between the simulation parameters increases. It was proved that the Tukey test was stable and limited the inflation, the error type 1, when the number of treatments increases. Besides, it is the most adequate in multiple comparisons of this kind of experiment.

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