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Ferreira T.S.,Centro Universitario Of Belo Horizonte Unibh | Moreira C.Z.,Centro Universitario Of Belo Horizonte Unibh | Caria N.Z.,Centro Universitario Of Belo Horizonte Unibh | Victoriano G.,São Paulo State University | And 2 more authors.
Revista Brasileira de Plantas Medicinais | Year: 2014

There has been a great advance in the pharmacognosy field, which has increased the use of medicinal plants by health professionals and practitioners of folk medicine. This systematic review explored the history and the present day application of phytotherapeutic medicines. We searched the databases Cochrane Library, Embase, Lilacs, PubMed, Scielo and a specialized bibliography. We concluded that there are many therapeutic potentialities in the use of phytotherapeutic medicines and medicinal plants. We also concluded that despite the increasing number of publications in the field each year, there is still a lack of reviews and meta-analyses that could promote a better integration of the knowledge produced. In addition, research about pharmacological interactions and multidisciplinary studies may promote a quicker and safer process from the workbench up to the clinical trials.


Yatsu F.K.J.,Federal University of Rio Grande do Sul | Koester L.S.,Federal University of Rio Grande do Sul | Lula I.,Federal University of Minas Gerais | Lula I.,Centro Universitario Of Belo Horizonte Unibh | And 4 more authors.
Carbohydrate Polymers | Year: 2013

In the present study we evaluated the complexation of daidzein/genistein/ glycitein, present in an isoflavone enriched fraction (IEF), with β-cyclodextrin and 2-hydroxypropyl-β-cyclodextrin (HPβCD). Based on the increased solubility and higher complexation efficiency, IEF and HPβCD solid complexes were prepared by kneading, freeze-drying, co-evaporation, spray-drying and microwave. The solid complexes were characterized using Fourier transformed-infrared spectroscopy, differential scanning calorimetry, X-ray diffraction, scanning electron microscopy, and nuclear magnetic resonance spectroscopy, and the isoflavone content and solubility were determined by liquid chromatography. The results suggest that the isoflavones daidzein, genistein and glycitein may be externally associated to HPβCD as well as that isoflavones/HPβCD inclusion complexes are formed through the insertion of B-ring into the cyclodextrin cavity. Except for the freeze-dried IEF/HPβCD solid complex, all complexes showed similar content and solubility. In conclusion, the three isoflavones showed to be able to simultaneously complex with HPβCD. © 2013 The Authors. Published by Elsevier Ltd. All rights reserved.


Couto B.R.G.M.,Centro Universitario Of Belo Horizonte Unibh | Santoro M.M.,Federal University of Minas Gerais | Ladeira A.P.,Centro Universitario | Dos Santos M.A.,Federal University of Minas Gerais
BIOINFORMATICS 2013 - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms | Year: 2013

The majority of actual methods for predicting the protein type a new sequence encode is based on alignments. We present a method that codifies sequences as dipeptide frequency vectors in R400 and uses information from known protein databases to build logistic regression models for protein prediction. In addition to calculate the probability of an unknown sequence being a specific class of protein, the method performs a feature selection, identifying dipeptides important to each protein group. We tested the method on 16 randomly groups of proteins chosen from Swiss-Prot. Assessments of the fit of logistic regression models were made on an independent dataset and by comparing discriminant results with BLAST, the basic local alignment search tool. Overall rate of correct protein classification ranged from 87% to 99%, and the sensitivity ranged from 61% to 99%, similar or better than BLAST. We observed that BLAST had difficult to identify short sequences, as Venom peptides, showing only 18% of correct classification of this group of proteins. Logistic model hit 96% in this case. Areas under the ROC curves were higher than 0.90 for all models. After achieving the logistic models, the problem to predict the protein type of a new sequence encode became very simple. Same analysis can be achieved for any other protein group. In addition to good results, better than BLAST program, there are two important issues in the proposed method: firstly, the modeling phase is made by a case-control study that do not use all database, but only samples for each target protein. This way the modeling becomes fast and adaptable to huge problems. The second and most important characteristic of this method is that, after the modeling phase, the entire system reduces to a few source code with an interface to receive queries, a subroutine to encoded amino acids sequences as frequency vectors and the logistic equations to predict probabilities. After the model is built there is no more database searching or any comparison among the new sequence and known proteins. Copyright © 2013 SCITEPRESS - Science and Technology Publications.


Pereira H.O.,Federal University of Minas Gerais | Rezende E.M.,Federal University of Minas Gerais | Couto B.R.G.M.,Centro Universitario Of Belo Horizonte Unibh
Revista Brasileira de Ortopedia | Year: 2015

Objective To analyze infections of the surgical site among patients undergoing clean-wound surgery for correction of femoral fractures. Methods This was a historical cohort study developed in a large-sized hospital in Belo Horizonte. Data covering the period from July 2007 to July 2009 were gathered from the records in electronic medical files, relating to the characteristics of the patients, surgical procedures and surgical infections. The risk factors for infection were identified by means of statistical tests on bilateral hypotheses, taking the significance level to be 5%. Continuous variables were evaluated using Student's t test. Categorical variables were evaluated using the chi-square test, or Fisher's exact test, when necessary. For each factor under analysis, a point estimate and the 95% confidence interval for the relative risk were obtained. In the final stage of the study, multivariate logistic regression analysis was performed. Results 432 patients who underwent clean-wound surgery for correcting femoral fractures were included in this study. The rate of incidence of surgical site infections was 4.9% and the risk factors identified were the presence of stroke (odds ratio, OR = 5.0) and length of preoperative hospital stay greater than four days (OR = 3.3). Conclusion To prevent surgical site infections in operations for treating femoral fractures, measures involving assessment of patients' clinical conditions by a multiprofessional team, reduction of the length of preoperative hospital stay and prevention of complications resulting from infections will be necessary. © 2015 Sociedade Brasileira de Ortopedia e Traumatologia.


Silva M.R.M.A.,Federal University of Minas Gerais | Henriques J.G.B.,Federal University of Minas Gerais | Silva J.H.,Federal University of Minas Gerais | Camargos V.R.,Centro Universitario Of Belo Horizonte Unibh | Moreira P.R.,Federal University of Minas Gerais
Journal of Morphological Sciences | Year: 2016

Introduction: The knowledge of morphological variations of the veins of the head and neck is essential for health professionals, both for diagnostic procedures as for clinical and surgical planning. This study described changes in the following structures: retromandibular vein and its divisions, including the relationship with the facial nerve, facial vein, common facial vein and jugular veins. Material and Methods: The variations of the veins were analyzed in three heads, five hemi-heads (right side) and two hemi-heads (left side) of unknown age and sex. Results: The changes only on the right side of the face were: union between the superficial temporal and maxillary veins at a lower level; absence of the common facial vein and facial vein draining into the external jugular vein. While on the left, only, it was noted: posterior division of retromandibular, after unite with the common facial vein, led to the internal jugular vein; union between the posterior auricular and common facial veins to form the external jugular and union between posterior auricular and common facial veins to terminate into internal jugular. The absence of the anterior and posterior divisions of retromandibular vein was observed on both sides of the face. Conclusion: These findings provide relevant informations about important vessels of the head and neck and will contribute to the appropriate clinical and/or surgical planning, aiming at the preservation of important structures. © 2016, Brazilian Society of Anatomy. All rights reserved.


Araujo L.G.,Centro Universitario Of Belo Horizonte Unibh | Lima D.M.F.,Fisioterapeuta UniBH | Sampaio R.F.,Federal University of Minas Gerais | Pereira L.S.M.,Federal University of Minas Gerais
Revista Brasileira de Fisioterapia | Year: 2010

Background: Pain is considered a multidimensional experience and is very common in the elderly. The pain locus of control has become essential to understand how the perceptions, expectations and beliefs are related to individual behavior, attitudes, coping and adherence of the elderly with regards to the health conditions and with the proposed treatment. Studies focused on adaptation and reliability of instruments are necessary for health professionals. Objectives: The present study performed the cross-cultural adaptation of the Pain Locus of Control Scale (C form of Multidimensional Health Locus of Control) for Brazil and assessed its intra and inter-examiner reliability among a sample of 68 elderly individuals with non-oncological pain living in the community. Methods: The cross-cultural adaptation of the scale was performed using the methodology standardized by Beaton et al. (2000)*. Pearson's correlation coeffi cient (PCC) and the intraclass correlation coeffi cient (ICC) were used for the statistical analysis (p≤0.05). Results: Average age of the subjects was 69.6 ± 5.5 years; most were women, with low levels of income and education. The average pain duration was 10.2 years and the main clinical diagnosis was osteoarthritis. The reliability of the scale was adequate, with a regular to very strong correlations (PCC = 0.60 to 0.93) and a moderate to nearly perfect ICC (0.60 to 0.93), in mainly the chance locus of control and medical and healthcare professionals locus of control subscales. Conclusion: An adequate reliability and applicability was observed in our sample after adjustments and adaptations of the scale for use in elderly. © Revista Brasileira de Fisioterapia.


PubMed | Centro Universitario Of Belo Horizonte Unibh, Georgetown University and Federal University of Minas Gerais
Type: | Journal: Neuropeptides | Year: 2016

To determine if preeclampsia (PE) is associated with dysregulation of the neuropeptide Y (NPY) system.The study enrolled 114 subjects either with normal pregnancy (NP) or with PE. Systolic blood pressure (SBP) was collected from patients using a standard sphygmomanometer. The PE patients were divided into two groups based on the gestational age (GA) at delivery - placental PE (PLPE, GA <34 weeks) or maternal PE (MTPE, GA 34 weeks). NPY was measured in platelet rich plasma (PRP), platelet poor plasma (PPP) and in the serum of NP and PE patients utilizing radioimmunoassay. Serum levels of soluble fms-like tyrosine kinase-1 (sFlt-1) and placental growth factor (PlGF) were measured in NP and PE subjects by ELISA.SBP was higher in PE compared to NP. Circulating NPY in serum and PRP, as well as NPY content per 100,000 platelets, but not its concentrations in PPP, were elevated in PE, as compared to NP. The highest NPY concentrations were observed in sera and PRP of patients with MTPE. PE patients had also elevated levels of sFlt-1, as compared to NP, although no difference between PLPE and MTPL groups were observed. There was no increase in P1GF in PE patients.Systemic NPY is elevated in PE patients, as compared to NP. This increase is observed in blood fractions containing platelets, suggesting accumulation of the peptide in these cells. NPY concentrations are particularly high in patients with MTPE, underlying differences in etiology between PLPE and MTPE. Our study implicates NPY as a potential target in antihypertensive therapies for PE patients.


Hurtado R.,Centro Universitario Of Belo Horizonte Unibh | Geber S.,Federal University of Minas Gerais
Journal of Endometriosis | Year: 2012

Purpose: Laparoscopy is a very accurate diagnostic tool for endometriosis. However, diagnostic accuracy has been questioned, as there is no reliable link between the visual status of the peritoneal cavity reported by laparoscopy and the patient's clinical condition. In addition, there is no actual association between the presence of typical peritoneal lesions and its confirmation by means of pathologic examination. The aim of our study was to determine whether histologic microscopic assessment is a reliable method for the diagnosis of endometriosis. Methods: We performed a prospective transversal study with 68 women with suggestive history of endometriosis who underwent laparoscopy. After biopsy, an intra-observer and inter-observer variability of the histologic study was analyzed by the Kappa integrity test to reveal whether microscopic examination is an accurate exam for the diagnosis of endometriosis. Results: In 38 of the 68 slides, all examiners confirmed the presence of endometriosis (55.9%). The intra-observer analysis showed an individual agreement degree over 92 (kappa=0.85; P<.001), and the inter-observer analysis showed an overall agreement of 76.5 (k=0.78; P<.001). Conclusions: The results showed a high level of integrity of the histologic exam for the diagnosis of endometriosis and confirmed that microscopic histology is a reliable and reproducible diagnostic tool for endometriosis. © 2012 Wichtig Editore.


Ribeiro G.B.,Centro Universitario Of Belo Horizonte Unibh | Coelho A.L.D.,Centro Universitario Of Belo Horizonte Unibh | Chaves P.H.P.,Centro Universitario Of Belo Horizonte Unibh | Macedo R.L.,Centro Universitario Of Belo Horizonte Unibh | Silva T.A.B.,Centro Universitario Of Belo Horizonte Unibh
Revista Brasileira de Oftalmologia | Year: 2015

Objectives: To describe and analyze the prevalence of visual impairment and its causes in public school students in Belo Horizonte/MG (Brazil). Methods: Cross-sectional study of 1452 public school children from 5 to 19 years old, in Belo Horizonte/MG (Brazil), screened and evaluated between 2011 and 2014. Results: Of the total sample, 150 patients (10,33%) had low visual acuity at the screening, and were referred to an ophthalmologist to further examination. Forty (26%) of the referred patients presented at the ambulatory, where 29 (72,5%) of these showed indication of visual correction. Simple and compound myopic astigmatism were the most frequent diagnoses associated with prescription glasses. Conclusion: The study showed that the prevalence of visual impairment in this population were consistent with other similar programs. By contrast, the abstention rate was much higher than the expectation, showing the importance of preventive and informative actions about the eye health care.


Volpe C.M.O.,Hospital Santa Casa Of Belo Horizonte | Abreu L.F.M.,Hospital Santa Casa Of Belo Horizonte | Gomes P.S.,Hospital Santa Casa Of Belo Horizonte | Gonzaga R.M.,Hospital Santa Casa Of Belo Horizonte | And 2 more authors.
Oxidative Medicine and Cellular Longevity | Year: 2014

We examined nitric oxide (NO), IL-6, and TNF- α secretion from cultured palmitate-stimulated PBMNCs or in the plasma from type 2 diabetes mellitus (T2MD) patients or nondiabetic (ND) controls. Free fatty acids (FFA) have been suggested to induce chronic low-grade inflammation, activate the innate immune system, and cause deleterious effects on vascular cells and other tissues through inflammatory processes. The levels of NO, IL-6, TNF- α, and MDA were higher in supernatant of palmitate stimulated blood cells (PBMNC) or from plasma from patients. The results obtained in the present study demonstrated that hyperglycemia in diabetes exacerbates in vitro inflammatory responses in PBMNCs stimulated with high levels of SFA (palmitate). These results suggest that hyperglycemia primes PBMNCs for NO, IL-6, and TNF-alpha secretion under in vitro FFA stimulation are associated with the secretion of inflammatory biomarkers in diabetes. A combined therapy targeting signaling pathways activated by hyperglycemia in conjunction with simultaneous control of hyperglycemia and hypertriglyceridemia would be suggested for controlling the progress of diabetic complications. © 2014 Caroline Maria Oliveira Volpe et al.

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