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Yatsu F.K.J.,Federal University of Rio Grande do Sul | Koester L.S.,Federal University of Rio Grande do Sul | Lula I.,Federal University of Minas Gerais | Lula I.,Centro Universitario Of Belo Horizonte Unibh | And 4 more authors.
Carbohydrate Polymers | Year: 2013

In the present study we evaluated the complexation of daidzein/genistein/ glycitein, present in an isoflavone enriched fraction (IEF), with β-cyclodextrin and 2-hydroxypropyl-β-cyclodextrin (HPβCD). Based on the increased solubility and higher complexation efficiency, IEF and HPβCD solid complexes were prepared by kneading, freeze-drying, co-evaporation, spray-drying and microwave. The solid complexes were characterized using Fourier transformed-infrared spectroscopy, differential scanning calorimetry, X-ray diffraction, scanning electron microscopy, and nuclear magnetic resonance spectroscopy, and the isoflavone content and solubility were determined by liquid chromatography. The results suggest that the isoflavones daidzein, genistein and glycitein may be externally associated to HPβCD as well as that isoflavones/HPβCD inclusion complexes are formed through the insertion of B-ring into the cyclodextrin cavity. Except for the freeze-dried IEF/HPβCD solid complex, all complexes showed similar content and solubility. In conclusion, the three isoflavones showed to be able to simultaneously complex with HPβCD. © 2013 The Authors. Published by Elsevier Ltd. All rights reserved. Source

Couto B.R.G.M.,Centro Universitario Of Belo Horizonte Unibh | Santoro M.M.,Federal University of Minas Gerais | Ladeira A.P.,Centro Universitario | Dos Santos M.A.,Federal University of Minas Gerais
BIOINFORMATICS 2013 - Proceedings of the International Conference on Bioinformatics Models, Methods and Algorithms | Year: 2013

The majority of actual methods for predicting the protein type a new sequence encode is based on alignments. We present a method that codifies sequences as dipeptide frequency vectors in R400 and uses information from known protein databases to build logistic regression models for protein prediction. In addition to calculate the probability of an unknown sequence being a specific class of protein, the method performs a feature selection, identifying dipeptides important to each protein group. We tested the method on 16 randomly groups of proteins chosen from Swiss-Prot. Assessments of the fit of logistic regression models were made on an independent dataset and by comparing discriminant results with BLAST, the basic local alignment search tool. Overall rate of correct protein classification ranged from 87% to 99%, and the sensitivity ranged from 61% to 99%, similar or better than BLAST. We observed that BLAST had difficult to identify short sequences, as Venom peptides, showing only 18% of correct classification of this group of proteins. Logistic model hit 96% in this case. Areas under the ROC curves were higher than 0.90 for all models. After achieving the logistic models, the problem to predict the protein type of a new sequence encode became very simple. Same analysis can be achieved for any other protein group. In addition to good results, better than BLAST program, there are two important issues in the proposed method: firstly, the modeling phase is made by a case-control study that do not use all database, but only samples for each target protein. This way the modeling becomes fast and adaptable to huge problems. The second and most important characteristic of this method is that, after the modeling phase, the entire system reduces to a few source code with an interface to receive queries, a subroutine to encoded amino acids sequences as frequency vectors and the logistic equations to predict probabilities. After the model is built there is no more database searching or any comparison among the new sequence and known proteins. Copyright © 2013 SCITEPRESS - Science and Technology Publications. Source

Pereira H.O.,Federal University of Minas Gerais | Rezende E.M.,Federal University of Minas Gerais | Couto B.R.G.M.,Centro Universitario Of Belo Horizonte Unibh
Revista Brasileira de Ortopedia | Year: 2015

Objective To analyze infections of the surgical site among patients undergoing clean-wound surgery for correction of femoral fractures. Methods This was a historical cohort study developed in a large-sized hospital in Belo Horizonte. Data covering the period from July 2007 to July 2009 were gathered from the records in electronic medical files, relating to the characteristics of the patients, surgical procedures and surgical infections. The risk factors for infection were identified by means of statistical tests on bilateral hypotheses, taking the significance level to be 5%. Continuous variables were evaluated using Student's t test. Categorical variables were evaluated using the chi-square test, or Fisher's exact test, when necessary. For each factor under analysis, a point estimate and the 95% confidence interval for the relative risk were obtained. In the final stage of the study, multivariate logistic regression analysis was performed. Results 432 patients who underwent clean-wound surgery for correcting femoral fractures were included in this study. The rate of incidence of surgical site infections was 4.9% and the risk factors identified were the presence of stroke (odds ratio, OR = 5.0) and length of preoperative hospital stay greater than four days (OR = 3.3). Conclusion To prevent surgical site infections in operations for treating femoral fractures, measures involving assessment of patients' clinical conditions by a multiprofessional team, reduction of the length of preoperative hospital stay and prevention of complications resulting from infections will be necessary. © 2015 Sociedade Brasileira de Ortopedia e Traumatologia. Source

Araujo L.G.,Centro Universitario Of Belo Horizonte Unibh | Lima D.M.F.,Fisioterapeuta UniBH | Sampaio R.F.,Federal University of Minas Gerais | Pereira L.S.M.,Federal University of Minas Gerais
Revista Brasileira de Fisioterapia | Year: 2010

Background: Pain is considered a multidimensional experience and is very common in the elderly. The pain locus of control has become essential to understand how the perceptions, expectations and beliefs are related to individual behavior, attitudes, coping and adherence of the elderly with regards to the health conditions and with the proposed treatment. Studies focused on adaptation and reliability of instruments are necessary for health professionals. Objectives: The present study performed the cross-cultural adaptation of the Pain Locus of Control Scale (C form of Multidimensional Health Locus of Control) for Brazil and assessed its intra and inter-examiner reliability among a sample of 68 elderly individuals with non-oncological pain living in the community. Methods: The cross-cultural adaptation of the scale was performed using the methodology standardized by Beaton et al. (2000)*. Pearson's correlation coeffi cient (PCC) and the intraclass correlation coeffi cient (ICC) were used for the statistical analysis (p≤0.05). Results: Average age of the subjects was 69.6 ± 5.5 years; most were women, with low levels of income and education. The average pain duration was 10.2 years and the main clinical diagnosis was osteoarthritis. The reliability of the scale was adequate, with a regular to very strong correlations (PCC = 0.60 to 0.93) and a moderate to nearly perfect ICC (0.60 to 0.93), in mainly the chance locus of control and medical and healthcare professionals locus of control subscales. Conclusion: An adequate reliability and applicability was observed in our sample after adjustments and adaptations of the scale for use in elderly. © Revista Brasileira de Fisioterapia. Source

Volpe C.M.O.,Nucleo de Pos Graduacao e Pesquisa NPGP | Abreu L.F.M.,Nucleo de Pos Graduacao e Pesquisa NPGP | Gomes P.S.,Nucleo de Pos Graduacao e Pesquisa NPGP | Gonzaga R.M.,Nucleo de Pos Graduacao e Pesquisa NPGP | And 2 more authors.
Oxidative Medicine and Cellular Longevity | Year: 2014

We examined nitric oxide (NO), IL-6, and TNF- α secretion from cultured palmitate-stimulated PBMNCs or in the plasma from type 2 diabetes mellitus (T2MD) patients or nondiabetic (ND) controls. Free fatty acids (FFA) have been suggested to induce chronic low-grade inflammation, activate the innate immune system, and cause deleterious effects on vascular cells and other tissues through inflammatory processes. The levels of NO, IL-6, TNF- α, and MDA were higher in supernatant of palmitate stimulated blood cells (PBMNC) or from plasma from patients. The results obtained in the present study demonstrated that hyperglycemia in diabetes exacerbates in vitro inflammatory responses in PBMNCs stimulated with high levels of SFA (palmitate). These results suggest that hyperglycemia primes PBMNCs for NO, IL-6, and TNF-alpha secretion under in vitro FFA stimulation are associated with the secretion of inflammatory biomarkers in diabetes. A combined therapy targeting signaling pathways activated by hyperglycemia in conjunction with simultaneous control of hyperglycemia and hypertriglyceridemia would be suggested for controlling the progress of diabetic complications. © 2014 Caroline Maria Oliveira Volpe et al. Source

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