Porto Alegre, Brazil
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Guimaraes A.C.,Centro Universitario Metodista do | Guimaraes A.C.,Federal University of Rio Grande do Sul | Wolfart M.,Federal University of Rio Grande do Sul | Brisolara M.L.L.,Federal University of Rio Grande do Sul | Dani C.,Centro Universitario Metodista do
Clinical Biochemistry | Year: 2012

Objective: Errors in clinical laboratories have a great impact on safety and care of patients. The pre-analytical phase is responsible for about 70% of errors. Hence, the objective was to determine what types of pre-analytical errors for improvements at this stage, and contribute data to the literature. Design and methods: Every three months during the study, the rejected blood samples were assessed for type of pre-analytical error and home care. Results: 77,051 blood samples were collected in the search period, whereof 441 (0.57%) were rejected by some type of pre-analytical error and therefore had to be recollected. Clot was found to be the major cause of rejection of samples, 43.8%, followed by insufficient sample volume, 24%. Conclusion: This study has shown that the most frequent causes of pre-analytical errors and rejection of samples corroborate those found in the literature. © 2011 The Canadian Society of Clinical Chemists.


Rodrigues A.D.,University of Caxias do Sul | Scheffel T.B.,University of Caxias do Sul | Scola G.,University of Caxias do Sul | dos Santos M.T.,Centro Universitario Metodista do | And 6 more authors.
Nutrition Research | Year: 2013

Oxidative damages in hepatocytes may be caused by epilepsy and/or anticonvulsant drugs. Epilepsy is one of the most common neurological disorders, characterized by recurrent seizures, which may increase the content of reactive oxygen species. Organic and conventional grape juices are rich in polyphenols, compounds with important antioxidant activity. It is hypothesized that organic and conventional purple grape juices may have protective effect against oxidative damage induced by pentylenetetrazole (PTZ) (a standard convulsant drug) in the liver and serum of Wistar rats. Animals (n = 16 in each group) received, by gavage, saline, organic grape juice or conventional grape juice (10 μL/g of body weight) for 17 days. Subsequently, half of the rats in each group received PTZ (60 mg/kg). After 30 minutes, the animals were euthanized by decapitation. Liver and blood samples were isolated to evaluate oxidative parameters (lipid and protein oxidation, nitric oxide metabolite content, antioxidant defenses, and protein sulfhydryl content). The results of this study showed that although organic juice contains higher polyphenol content than conventional juice, both juices conferred protection against lipid and protein oxidative damage and limited the increase in PTZ-induced nitric oxide metabolite content in the liver and serum. In addition, both juices inhibited the PTZ-induced reduction in enzymatic antioxidant defenses (superoxide dismutase and catalase activities) and sulfhydryl protein content in the liver and serum. In summary, both organic and conventional grape juices were able to reduce oxidative damage induced by PTZ in the liver and serum of Wistar rats. © 2013 Elsevier Inc.


Marinho K.F.,Centro Universitario Metodista do | Ratto C.G.,Centro Universitario La Salle
Saude e Sociedade | Year: 2016

Assuming that the borderline case seems to be a new “normality” today, this article discusses the contemporary society in its ways of life and the impact on the working world. The constant search for success and achievement consequently go through labor relations, which constitute the subject. No time for leisure! We have to produce. For the discussion we considered what we call “borderline case”, elaborated from its phenomenological and non-structural dimension, and from the paradigm of individual psychopathology, but as a symptom of the culture in a society marked by impulsiveness, fragility of social bonds and the so-called “culture of narcissism”. Through a psychoanalytic approach and brief description of the development of working methods and production over the years, we proposed a discussion of these new life dynamics involved in the working world, thinking, above all, in how this relationship is constructed in contemporaneity, in the context of a flexible capitalism. © 2016, UNIV SAOPAULO. All rights reserved.


Loss E.S.,Federal University of Rio Grande do Sul | Jacobus A.P.,Federal University of Rio Grande do Sul | Jacobus A.P.,Centro Universitario Metodista Do | Wassermann G.F.,Federal University of Rio Grande do Sul
Life Sciences | Year: 2011

This minireview describes the rapid signaling actions of follicle stimulating hormone (FSH) and testosterone in immature Sertoli cells mainly related to Ca 2+ inflow and the electrophysiological changes produced by hormones. The rapid membrane actions of FSH occur in a time frame of seconds to minutes, which include membrane depolarization and the stimulation of 45Ca 2+ uptake. These effects can be prevented by pertussis toxin (PTX), suggesting that they are likely mediated by Gi-protein coupled receptor activation. Furthermore, these effects were inhibited by verapamil, a blocker of the L-type voltage-dependent Ca 2+ channel (VDCC). Finally, FSH stimulation of 45Ca 2+ uptake was inhibited by the (phosphoinositide 3-kinase) PI3K inhibitor wortmannin. These results suggest that the rapid action of FSH on L-type Ca 2+ channel activity in Sertoli cells from pre-pubertal rats is mediated by the Gi/Gβγ/ PI3Kγ pathway, independent of its effects on insulin-like growth factor type I (IGF-I). Testosterone depolarizes the membrane potential and increases the resistance and the 45Ca 2+ uptake in Sertoli cells of the seminiferous tubules of immature rats. These actions were nullified by diazoxide (K + ATP channel opener). Testosterone actions were blocked by both PTX and the phospholipase C (PLC) inhibitor U73122, suggesting the involvement of PLC - phosphatidylinositol 4-5 bisphosphate (PIP2) hydrolysis via the Gq protein in the testosterone-mediated pathway. These results indicate that testosterone acts on the Sertoli cell membrane through the K + ATP channels and PLC-PIP2 hydrolysis, which closes the channel, depolarizes the membrane and stimulates 45Ca 2+ uptake. These results demonstrate the existence of rapid non-classical pathways in immature Sertoli cells regulated by FSH and testosterone. © 2011 Elsevier Inc.


Jacobus A.P.,Federal University of Rio Grande do Sul | Jacobus A.P.,Centro Universitario Metodista do | Loss E.S.,Federal University of Rio Grande do Sul | Wassermann G.F.,Federal University of Rio Grande do Sul
Frontiers in Physiology | Year: 2010

The aim of this study was to evaluate the effect of pertussis toxin (PTX) on the depolarizing component of the action of follicle stimulating hormone (FSH) on the membrane potential (MP) of Sertoli cells, which is linked to the rapid entry of Ca 2+ into cells and to the Ca 2+-dependent transport of neutral amino acids by the A system. This model allowed us to analyze the involvement of Gi proteins in the action of FSH in these phenomena. In parallel, using an inactive analog of insulin-like growth factor type I (IGF-1), JB1, and an anti-IGF-I antibody we investigated the possible mediating role of IGF-I on these effects of FSH because IGF-I is produced and released by testicular cells in response to stimulation by FSH and shows depolarization effects on MP similar to those from FSH. Our results have the following implications: (a) the rapid membrane actions of FSH, which occur in a time-frame of seconds to minutes and include the depolarization of the MP, and stimulation of 45Ca 2+ uptake and [ 14C]-methyl aminoisobutyric acid ([ 14C]-MeAIB) transport, are nullified by the action of PTX and, therefore, are probably mediated by GiPCR activation; (b) the effects of FSH were also nullified by verapamil, an L-type voltage- dependent Ca 2+ channel blocker; (c) wortmannin, an inhibitor of phosphoinositide 3-kinase (PI3K), prevented FSH stimulation of 45Ca 2+ entry and [ 14C]-MeAIB transport; and (d) these FSH actions are independent of the IGF-I effects. In conclusion, these results strongly suggest that the rapid action of FSH on L-type Ca 2+ channel activity in Sertoli cells from 10- to 12-day-old rats is mediated by the Gi/βγ/PI3Kγ pathway, independent of the effects of IGF-I. © 2010 Jacobus, Loss and Wassermann.


Escott G.M.,Federal University of Rio Grande do Sul | De Castro A.L.,Federal University of Rio Grande do Sul | Jacobus A.P.,Centro Universitario Metodista Do | Loss E.S.,Federal University of Rio Grande do Sul
Biochimica et Biophysica Acta - Biomembranes | Year: 2014

Insulin and insulin-like growth factor 1 (IGF-I) are capable of activating similar intracellular pathways. Insulin acts mainly through its own receptor, but can also activate the IGF-I receptor (IGF-IR). The aim of this study was to investigate the involvement of the IGF-IR in the effects of insulin and IGF-I on the membrane potential of immature Sertoli cells in whole seminiferous tubules, as well as on calcium, amino acid, and glucose uptake in testicular tissue of immature rats. The membrane potential of the Sertoli cells was recorded using a standard single microelectrode technique. In calcium uptake experiments, the testes were pre-incubated with 45Ca2 +, with or without JB1 (1 μg/mL), and then incubated with insulin (100 nM) or IGF-I (15 nM). In amino acid and glucose uptake experiments, the gonads were pre-incubated with or without JB1 (1 μg/mL) and then incubated with radiolabeled amino acid or glucose analogues in the presence of insulin (100 nM) or IGF-I (15 nM). The blockade of IGF-IR with JB1 prevented the depolarising effects of both insulin and IGF-I on membrane potential, as well as the effect of insulin on calcium uptake. JB1 also inhibited the effects of insulin and IGF-I on glucose uptake. The effect of IGF-I on amino acid transport was inhibited in the presence of JB1, whereas the effect of insulin was not. We concluded that while IGF-I seems to act mainly through its cognate receptor to induce membrane depolarisation and calcium, amino acid and glucose uptake, insulin appears to be able to elicit its effects through IGF-IR, in seminiferous tubules from immature rats. © 2014 Elsevier B.V.


Escott G.M.,Federal University of Rio Grande do Sul | Jacobus A.P.,Centro Universitario Metodista Do | Loss E.S.,Federal University of Rio Grande do Sul
Pflugers Archiv European Journal of Physiology | Year: 2013

There is clear evidence that insulin and insulin-like growth factor I (IGF-I) are crucial for the normal metabolism and development of Sertoli cells. However, the mechanisms of insulin regulatory signaling remain unknown in these cells, especially during the immature period. The aim of this study was to investigate the electrophysiological effects of insulin and the effects of insulin and IGF-I on calcium uptake, amino acid, and glucose transport in whole seminiferous tubules from 12-day-old rats, as well as the involvement of PI3K/Akt signaling pathway in these effects. Insulin produces a depolarizing effect on the membrane potential of Sertoli cells in seminiferous tubules within 180 s. This effect was nullified by verapamil, an L-type voltage-dependent calcium channel blocker, therefore demonstrating a calcium-dependent depolarizing effect. Both insulin and IGF-I stimulate calcium uptake, amino acid, and glucose transport in whole testes from 12-day-old rats. These stimulatory effects of insulin and IGF-I on calcium uptake and amino acid and glucose transport on testicular tissue were nullified by wortmannin, which demonstrates the involvement of the PI3K/Akt signaling pathway in these hormonal effects. © 2013 Springer-Verlag Berlin Heidelberg.


Caletti G.,Federal University of Health Sciences, Porto Alegre | Almeida F.B.,Federal University of Health Sciences, Porto Alegre | Agnes G.,Federal University of Health Sciences, Porto Alegre | Nin M.S.,Federal University of Health Sciences, Porto Alegre | And 4 more authors.
Behavioural Brain Research | Year: 2015

Diabetes mellitus is a metabolic disorder associated with higher risk for depression. Diabetic rats present depressive-like behaviors and taurine, one of the most abundant free amino acids in the brain, reverses this depressive behaviors. Because taurine is a GABAA agonist modulator, we hypothesize that its antidepressant effect results from the interaction on this system by changing α2 GABAA receptor subunit expression, beside changes on BDNF mRNA, and memory in diabetic rats. Streptozotocin-diabetic and non-diabetic Wistar rats were daily injected with 100mg/kg of taurine or saline, intraperitoneally, for 30 days. At the end of the experiment, rats were exposed to the novel object recognition memory. Later they were euthanized, the brains were weighed, and the hippocampus was dissected for α2 GABAA subunit and BDNF mRNA expression. Real-time quantitative PCR (qPCR) showed that diabetic rats presented lower α2 GABAA subunit and BDNF mRNA expression than non-diabetic rats and taurine increased both parameters in these sick rats. Taurine also reversed the lower brain weight and improved the short-term memory in diabetic rats. Thus, the taurine antidepressant effect may be explained by interference with the GABA system, in line to its neuroprotective effect showed here by preventing brain weight loss and improving memory in diabetic rats. © 2015 Elsevier B.V.


Pagano M.,Federal University of Rio Grande do Sul | Martins A.F.,Centro Universitario Metodista Do | MacHado A.B.M.P.,Hospital Of Clinicas Of Porto Alegre | Barin J.,Federal University of Rio Grande do Sul | And 2 more authors.
Epidemiology and Infection | Year: 2013

Over the last decade, Acinetobacter baumannii resistant to carbapenems has emerged in many medical centres and is commonly associated with high morbidity and mortality. We investigated potential mechanisms contributing to antimicrobial resistance of 58 clinical isolates of A. baumannii collected during a prolonged city-wide outbreak in five different hospitals in southern Brazil. The integrase gene was detected in 51 (87·9%) isolates of which 36 harboured class 2 integrons alone and 14 had both class 1 and 2 integrons; all carbapenem-resistant isolates displayed class 2 integrons. ISAba1 was found upstream of bla OXA-23-like only in isolates resistant to carbapenems; however, ISAba1 upstream of bla OXA-51-like was present in both susceptible and resistant isolates. This is the first report of a high prevalence of class 2 integrons in A. baumannii in southern Brazil. Moreover, our study suggests that ISAba1/bla OXA-51-like alone is insufficient to confer resistance to carbapenems. © 2012 Cambridge University Press.


PubMed | Federal University of Rio Grande do Sul and Centro Universitario Metodista do
Type: Journal Article | Journal: Antioxidants (Basel, Switzerland) | Year: 2016

The objective of this study was to investigate the antioxidant and hepatoprotective effect of the chronic use of conventional (CGJ) or organic (OGJ) grape juice from the Bordeaux variety grape on oxidative stress and cytoarchitecture in the liver of rats supplemented with a high-fat diet (HFD) for three months. The results demonstrated that HFD induced an increase in thiobarbituric acid-reactive substances (TBARS), catalase (CAT) activity and 2,7-dihydrodichlorofluorescein (DCFH) oxidation and a decrease in sulfhydryl content and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities. HFD also induced hepatocellular degeneration and steatosis. These alterations were prevented by CGJ and OGJ, where OGJ was more effective. Therefore, it was concluded that HFD induced oxidative stress and liver damage and that the chronic use of grape juice was able to prevent these alterations.

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