Centro Universitario Herminio Ometto

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Centro Universitario Herminio Ometto

Brazil

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Campos-Pereira F.D.,Centro Universitario Herminio Ometto | Oliveira C.A.,Centro Universitario Herminio Ometto | Pigoso A.A.,Centro Universitario Herminio Ometto | Silva-Zacarin E.C.M.,Federal University of São Carlos | And 4 more authors.
Ecotoxicology and Environmental Safety | Year: 2012

Risk assessments suggest that intermediate and long-term exposure to triazine herbicides and its metabolites through water can cause severe damage to human health. The objective of this study was to investigate the possible effects of atrazine on Wistar rats submitted to subacute treatment. For this purpose, the activity of catalase and alanine aminotransferase was quantified, and the effect of the herbicide on cell membranes was examined based on the measurement of lipid peroxidation and consequent formation of malondialdehyde and on the mRNA expression of antioxidant enzymes (Mn-superoxide dismutase [SOD] and GSTM1) and connexins. In addition, we evaluated histopathological alterations in the liver, cellular expression of SOD and glutathione (GST), activation of heat shock proteins (HSPs) by immunohistochemistry, and the induction of apoptosis. The genotoxic potential of the herbicide was investigated by the micronucleus test in bone marrow smears. Adult male Wistar rats were treated with an aqueous solution of atrazine at a concentration of 400. mg/kg/day, by gavage, for 14 consecutive days. Control groups were also included. The results showed an increase of catalase levels and maintenance of the expression of antioxidant enzymes (SOD and GST). In addition, lipid peroxidation, hepatic tissue degeneration, activation of HSP90, increased levels of connexin mRNA, and genotoxicity were observed. In conclusion, atrazine induced early hepatic oxidative stress that triggered defense mechanisms to maintain the morphophysiological integrity of the liver. Further studies are needed to better understand the effects of this herbicide on human health. © 2011 Elsevier Inc.


Clerici M.T.P.S.,Federal University of Alfenas | Kallmann C.,Centro Universitario Herminio Ometto | Gaspi F.O.G.,Centro Universitario Herminio Ometto | Morgano M.A.,Institute Tecnologia Of Alimentos Ital | And 2 more authors.
Food Research International | Year: 2011

Fruit of wolf (Solanum lycocarpum A. St. - HILL), found in Brazilian cerrado, has been used in the initial ripening stage as flour and/or starch in popular medicine due to its hypoglycemiant action. The aim of this work was to study the occurrence of phytochemical compounds, physical, chemical and technological characteristics of fruit of wolf flour and starch in the initial ripening stage. Flour and starch were extracted using known popularly and experimental methods (with sodium bisulfite) and chemical composition and technological characteristics were analyzed. The results were comparatively assessed by Tukey's test (p < 0.05). The recommendation for use was the extraction of flour and starch products by previously removing peels and seeds of fruit of wolf. The most relevant finding in this study is the high content of fibers in flour (23. g/100. g) and high content of resistant starch (32. g/100. g) in the fraction of starch extracted from fruit of wolf, which can explain their use as hypoglycemic agent. © 2011 Elsevier Ltd.


Blay S.L.,University of Sao Paulo | Marchesoni M.S.M.,Centro Universitario Herminio Ometto
Cadernos de Saude Publica | Year: 2011

The aim of this study is to investigate the impact of psychiatric morbidity, depression, cognitive deficit, number of self-reported illnesses and socio-demographic variables on the WHOQOLBref domain scores. WHOQOL-Bref domain scores are substantially affected by psychiatric morbidity and income. Depression, the number of selfreported illnesses and the female gender also explain the variability of other domains to a lesser extent.


Andreoli C.P.P.,Centro Universitario Herminio Ometto | De Martino M.M.F.,University of Campinas
Sleep Science | Year: 2012

Objectives: The assessment of student achievement in the teaching-learning is a constant concern of the study professors. Actually the night shift is a reality in favor of the persons seeking qualification for a position in the labor market. This study aimed to evaluate the academic performance and correlate with the indices of student achievement and sleep patterns. Methods: Participants were college students from the University Center Herminio Ometto, UNIARARAS, SP, (n = 139) with a mean age of 23.59 years. Were used: a questionnaire to gather personal information and professional evaluation of sleep-wake cycle (sleep diaries), validated by Benedito-Silva et al. (1990). For the analysis of academic performance, we used the Scheduled System Assessment (SPA). Results: The sleep patterns were statistically different between groups (Mann-Whitney) for the variables bedtime (p < 0.001), bedtime (p <0.001), time to wake up (p <0.001) and duration nap (p = 0.009). As for academic performance, students in the daytime period had higher mean values when compared with the group the night. Conclusion: The group of diurnal periods showed better academic performance in comparison with that of the night. The later the hour of waking, the lower academic performance, suggesting the effect of nighttime sleep and deficits. The sleep quality was better for the group of the day.


Medrano R.F.V.,Centro Universitario Herminio Ometto | De Oliveira C.A.,Centro Universitario Herminio Ometto
Molecular Biotechnology | Year: 2014

The tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR) reaction is a simple and economical method to genotype single-nucleotide polymorphisms (SNPs). It uses four primers in a single PCR and is followed just by gel electrophoresis. However, the optimization step can be very hardworking and time-consuming. Hence, we propose to demonstrate and discuss critical steps for its development, in a way to provide useful information. Two SNPs that provided different amplification conditions were selected. DNA extraction methods, annealing temperatures, PCR cycles protocols, reagents, and primers concentration were also analyzed. The use of tetra-primer ARMS-PCR could be impaired for SNPs in DNA regions rich in cytosine and guanine and for samples with DNA not purified. The melting temperature was considered the factor of greater interference. However, small changes in the reagents concentration significantly affect the PCR, especially MgCl2. Balancing the inner primers band is also a key step. So, in order to balance the inner primers band, intensity is important to observe which one has the weakest band and promote its band by increasing its concentration. The use of tetra-primer ARMS-PCR attends the expectations of modern genomic research and allows the study of SNPs in a fast, reliable, and low-cost way. © 2014 Springer Science+Business Media.


Tessutti L.S.,University of Campinas | Macedo D.V.,University of Campinas | Kubota L.T.,University of Campinas | Alves A.A.,University of Campinas | Alves A.A.,Centro Universitario Herminio Ometto
Analytical Biochemistry | Year: 2013

The use of potentiometry to measure plasma antioxidant capacity to contribute to oxidative stress evaluation is presented. In this assay, plasma (n = 60) diluted (0.3 to 1 ml) in phosphate buffer, pH 7.4, NaCl 9%, was submitted to potentiometry. A platinum wire was the working electrode and saturated calomel the reference. The results are presented as the difference between sample and buffer potential (ΔE). ΔE presented a good inverse correlation with added increasing concentrations of ascorbate (2.5-75 μmol/L; R = -0.99), urate (9.0-150 μmol/L; R = -0.99), and bilirubin (0.78-13 μmol/L; R = -0.99). Increase in the antioxidant capacity decreased ΔE. Depletion of the antioxidant capacity by tert-butylhydroperoxide (6.5-50 μmol/L) presented a direct correlation (0.97) with ΔE. Furthermore, ΔE presented an inverse correlation (R = -0.99) with increased antioxidant capacity of plasma (FRAP) induced by the addition of ascorbate (2.5-75 μmol/L). The response of the potentiometric method proved be adequate for measuring the plasma antioxidant depletion induced by acute exhaustive exercise in rats (control, n = 15; exercised, n = 15). This exercise decreased the concentration of urate (p < 0.05), decreased FRAP (p < 0.5), increased TBARS (p < 0.5), and decreased the potentiometer sensor response (p = 6.5 × 10-3). These results demonstrate the adequacy of potentiometry for evaluating the antioxidant capacity of blood plasma samples. © 2013 Elsevier Inc. All rights reserved.


Rebelato H.J.,Centro Universitario Herminio Ometto | Esquisatto M.A.M.,Centro Universitario Herminio Ometto | Moraes C.,Centro Universitario Herminio Ometto | Amaral M.E.C.,Centro Universitario Herminio Ometto | Catisti R.,Centro Universitario Herminio Ometto
Journal of Molecular Histology | Year: 2013

The placenta acts a regulator of nutrient composition and supply from mother to fetus and is the source of hormonal signals that affect maternal and fetal metabolism. Thus, appropriate development of the placenta is crucial for normal fetal development. We investigated the effect of gestational protein restriction (GPR) on placental morphology and mitochondrial function on day 19 of gestation. Pregnant dams were divided into two groups: normal (NP 17 % casein) or low-protein diet (LP 6 % casein). The placentas were processed for biochemical, histomorphometric and ultrastructural analysis. The integrity of rat placental mitochondria (RPM) isolated by conventional differential centrifugation was measured by oxygen uptake (Clark-type electrode). LP animals presented an increase in adipose tissue and triacylglycerol and a decrease in serum insulin levels. No alterations were observed in body, liver, fetus, or placenta weight. There was also no change in serum glucose, total protein, or lipid content. Gestational protein restriction had tissue-specific respiratory effects, with the observation of a small change in liver respiration (∼13 %) and considerable respiratory inhibition in placenta samples (∼37 %). The higher oxygen uptake by RPM in the LP groups suggests uncoupling between respiration and oxidative phosphorylation. In addition, ultrastructural analysis of junctional zone giant cells from LP placenta showed a disorganized cytoplasm, with loss of integrity of most organelles and intense vacuolization. The present results led us to hypothesize that GPR alters placental structure and morphology, induces sensitivity to insulin, mitochondrial abnormalities and suggests premature aging of the placenta. Further studies are needed to test this hypothesis. © 2013 Springer Science+Business Media Dordrecht.


Pedroso de Moraes C.,Centro Universitario Herminio Ometto | de Souza-Leal T.,Centro Universitario Herminio Ometto | Brescansin R.L.,Centro Universitario Herminio Ometto | Pettini-Benelli A.,Federal University of Mato Grosso | Gracas Sajo M.D.,Claro
Anais da Academia Brasileira de Ciencias | Year: 2012

Considering that the root structure of the Brazilian genera belonging to the Catasetinae subtribe is poorly known, we describe the roots of twelve representatives from this subtribe. For anatomical analysis, the roots were fixed in FAA 50, preserved in ethanol 70% and sectioned at its medium region using razor blades. The sections were stained with 0.05% astra blue and safranin and mounted in glycerin. For the identification of starch we used Lugol ́s solution; for lignin, floroglucin chloridric; for lipids, Sudan III, and for flavanoids, potassium hydroxide. The relevant aspects were registered using a digital camera joined with an Olympus microspope (BX51 model). The structural similarities of all roots support the placement of the subtribe Catasetinae into the monophyletic tribe Cymbidieae. Some root features are restricted to one or two taxa and can be useful in the systematics of the subtribe. For example, the occurrence of flavonoidic crystals characterizes the genera Catasetum and Cychnodes, and the number of the velamen layers and the shape of the epivelamen cells are useful to confirm the taxonomic position of Clowesia amazonica. The presence of velamen and flavonoidic crystals was interpreted as an adaptation to the epiphytic habit.


Pires R.C.,Centro Universitario Herminio Ometto | Souza E.E.,Centro Universitario Herminio Ometto | Vanzela E.C.,University of Campinas | Ribeiro R.A.,Federal University of Rio de Janeiro | And 4 more authors.
Applied Physiology, Nutrition and Metabolism | Year: 2014

The occurrence of metabolic disorders, such as diabetes, obesity, atherosclerosis, and hypertension, increases with age. Inappropriate food intake, when combined with genetic and hormonal factors, can trigger the occurrence of these diseases in aged organisms. This study investigated whether short-term calorie restriction (CR; 40% of the intake of control animals (CTL) for 21 days) benefits 1-year-old (CR1yr) and 2-year-old (CR2yr) Wistar rats, with regard to insulin secretion and action. Plasma insulin and the insulin secreted by isolated islets were measured with radioimmunoassay, and the insulin sensitivity of peripheral tissues was assessed with the intraperitoneal glucose tolerance test (IPGTT), intraperitoneal insulin tolerance test, and hepatic and muscle adenosine monophosphate-activated protein kinase (AMPK) phosphorylation measurements. Body weight, epididymal fat pad, epididymal fat pad/body weight index, plasma glucose, and insulin were lower in the CR1yr than in the control (CTL1yr) rats. Serum cholesterol, triglycerides, and protein, as well as hepatic and muscle glycogen content, were similar between the CR and CTL groups. The IPGTT was higher in CR2yr and CTL2yr rats than in CR1yr and CTL1yr rats, and insulin sensitivity was higher in CR1yr and CR2yr rats than in their respective CTLs. This was associated with an increase in hepatic and muscle AMPK phosphorylation. No differences in glucose-induced insulin secretion in the isolated islets were observed between CRs and their respective CTL rats. In conclusion, short-term calorie restriction provoked more severe alterations in CR1yr than CR2yr rats. The normoglycemia observed in both CR groups seems to be due to an increase in insulin sensitivity, with the involvement of liver and muscle AMPK.


Capucho C.,Centro Universitario Herminio Ometto | Sette R.,Centro Universitario Herminio Ometto | de Souza Predes F.,University Estadual do Parana | de Castro Monteiro J.,Federal University of Espirito Santo | And 4 more authors.
Food and Chemical Toxicology | Year: 2012

Basal generation of reactive oxygen species (ROS) is essential for male reproductive function, whereas high ROS levels may be linked to low quality of sperm and male infertility. The number of antioxidants known to inflict damage is growing, and it will be of interest to study natural products, which may have this activity. Since the epididymis is known to play an important role in providing the microenvironment for sperm maturation and storage of sperm, this study was undertaken to evaluate the morphometric-stereological and functional alterations in the epididymis after chronic treatment with low doses of Brazilian green propolis, which is known for its antioxidant properties. For this purpose, forty-eight adult male Wistar rats were treated with 3, 6 and 10. mg/kg/day of aqueous extract of Brazilian green propolis during 56. days and morphological parameters, sperm production and number of sperm in rat epididymis and oxidative stress levels were analyzed. The results showed higher sperm production and greater epithelium height of the epididymis initial segment and no induction of oxidative stress in treated animals. Further studies are needed to fully understand the effects of propolis on the reproductive system but our results showed that it could alter male reproductive function. © 2012 Elsevier Ltd.

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