Time filter

Source Type

Santos M.A.,Embrapa Soja | Geraldi I.O.,Escola Superior de Agricultura Luiz de Queiroz ESALQ | Garcia A.A.F.,Escola Superior de Agricultura Luiz de Queiroz ESALQ | Bortolatto N.,Embrapa Soja | And 4 more authors.
Hereditas | Year: 2013

Biological nitrogen fixation (BNF) is a key process, but despite the economic and environmental importance, few studies about quantitative trait loci (QTL) controlling BNF traits are available, even in the economically important crop soybean Glycine max (L.) Merr. In this study, a population of 157 F2:7 RILs derived from crossing soybean cultivars Bossier (high BNF capacity) and Embrapa 20 (medium BNF capacity) was genotyped with 105 simple sequence repeat markers (SSRs). The genetic map obtained has 1231.2 cM and covers about 50% of the genome, with an average interval of 18.1 cM. Three traits, nodule number (NN), the ratio nodule dry weight (NDW)/NN and shoot dry weight (SDW) were used to evaluate BNF performance. A composite interval mapping for multiple traits method (mCIM) analysis mapped two QTLs for SDW (LGs E and L), three for NN (LGs B1, E and I), and one for NDW/NN (LG I); all QTLs were of small effect (R-values ranging from 1.7% to 10.0%) and explained 15.4%, 13.8% and 6.5% of total variation for these three traits, respectively. © 2013 The Authors.


PubMed | University do Estado do Parana, State University of Maringá and Centro Universitario Filadelfia
Type: Journal Article | Journal: Epidemiologia e servicos de saude : revista do Sistema Unico de Saude do Brasil | Year: 2016

to describe near miss maternal morbidity among women living in Paran State, Brazil, in 2010.this was a descriptive study using Brazilian National Hospital Information System (SIH/SUS) data on all hospital admissions with primary diagnosis falling under Chapter XV of the International Statistical Classification of Diseases and Related Health Problems 10th Revision and/or with records of obstetric procedures indicative of near misses; three criteria were used to define severe maternal morbidity.4,890 admissions owing to near miss were identified, with a rate of 52.9 hospitalizations per 1,000 births, a rate of 69.8/1,000 among women aged 35-39 and a rate of 356.6/1,000 among women aged 44-49; the leading causes of hospitalization were preeclampsia (28.2%), haemorrhage (23.7%) and immune system dysfunction (14.0%).the results indicate the need to pay greater attention to women aged 35 and over since they had higher rates of near miss.


Cyrino Zequi J.A.,National Institute of Amazonian Research | dos Santos F.P.,Centro Universitario Filadelfia | Lopes J.,State University Londrina
Revista Colombiana de Entomologia | Year: 2014

Industrial or urban wastewater treatment lagoons accumulate nutrients, which allows the proliferation of Cx. quinquefasciatus, vector of the etiologic agent of filariasis, and of Cx. saltanensis, vector of the protozoan causing malaria in chickens. The goal of this study was to evaluate the effectiveness and persistence of Aquabac® XT 1,200 UTI/ mg, Teknar® 3,000 AAU/mg, and Vectobac® AS 1,200 UTI/mg; these products are liquid formulations whose active principles are crystals produced by Bacillus thuringiensis israelensis. Products were tested in two wastewater treatment lagoons of a meat cold-storage facility. The lagoons measured 1,419 m2 and 736 m2, and received concentrations of 1 and 2 litres/hectare of each product, with three replicates each. Water pH, conductivity, oxygen and temperature were measured at each collection. Cx. quinquefasciatus and Cx. saltanensis were found in both lagoons. One litre/hectare concentration controlled 70 to 80% of the larvae in 24 and 48 hours, respectively; two litres/hectare concentration showed the best results, controlling 86 to 99% of the immature mosquitoes, with the highest efficiency observed at 48 hours. None of the products affected water quality according to the abiotic parameters examined. As a result, all products tested efficiently controlled these Culicidae under the local breeding conditions but had low persistence, with seventh-day larval indexes being similar to those recorded before application of the products. Weekly applications are recommended. © 2014 The American Society for Cell Biology.


Gomes D.F.,Embrapa Soja | Gomes D.F.,State University Londrina | Batista J.S.D.S.,Embrapa Soja | Batista J.S.D.S.,Centro Universitario Filadelfia | And 5 more authors.
BMC Microbiology | Year: 2012

Background: Rhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of N-fertilizers. The objective of our study was to obtain an overview of adaptive responses to heat stress of strain PRF 81, by analyzing differentially expressed proteins when the bacterium is grown at 28°C and 35°C. Results: Two-dimensional gel electrophoresis (2DE) revealed up-regulation of fifty-nine spots that were identified by MALDI-TOF/TOF-TOF. Differentially expressed proteins were associated with the functional COG categories of metabolism, cellular processes and signaling, information storage and processing. Among the up-regulated proteins, we found some related to conserved heat responses, such as molecular chaperones DnaK and GroEL, and other related proteins, such as translation factors EF-Tu, EF-G, EF-Ts and IF2. Interestingly, several oxidative stress-responsive proteins were also up-regulated, and these results reveal the diversity of adaptation mechanisms presented by this thermotolerant strain, suggesting a cross-talk between heat and oxidative stresses. Conclusions: Our data provide valuable protein-expression information relevant to the ongoing genome sequencing of strain PRF 81, and contributes to our still-poor knowledge of the molecular determinants of the thermotolerance exhibited by R. tropici species. © 2012 Gomes et al.


Ishii P.L.,Centro Universitario Filadelfia | Ishii P.L.,São Paulo State University | Prado C.K.,Centro Universitario Filadelfia | Mauro M.D.O.,Centro Universitario Filadelfia | And 8 more authors.
Regulatory Toxicology and Pharmacology | Year: 2011

The development of various types of cancer results from the interaction among endogenous, environmental and hormonal factors, where the most notable of these factors is diet. The aim of the present study was to determine the antigenotoxic, anticarcinogenic, phagocytic and immunomodulatory activities of Agaricus blazei. The test antigenotoxicity (Comet Assay) and anticarcinogenic (Test of Aberrant Crypt Foci) assess changes in DNA and/or intestinal mucosa that correlate to cancer development. Tests of phagocytosis in the spleen and differential count in blood cells allow the inference of modulation of the immune system as well as to propose a way of eliminating cells with DNA damage. Supplementation with the mushroom was carried out under pre-treatment, simultaneous treatment, post-treatment and pre-treatment. +. continuous conditions. Statistical analysis demonstrated that the mushroom did not have genotoxic activity but showed antigenotoxic activity. Supplementation caused an increase in the number of monocytes and in phagocytic activity, suggesting that supplementation increases a proliferation of monocytes, consequently increasing phagocytic capacity especially in the groups pre-treatment, simultaneous and pre-treatment. +. continuous. The data suggest that A. blazei could act as a functional food capable of promoting immunomodulation which can account for the destruction of cells with DNA alterations that correlate with the development of cancer, since this mushroom was demonstrated to have a preventive effect against pre-neoplastic colorectal lesions evaluated by the aberrant crypt foci assay. According to these results and the literature, it is believed that supplementation with A. blazei can be an efficient method for the prevention of cancer as well as possibly being an important coadjuvant treatment in chemotherapy. © 2011 Elsevier Inc.


Lopes J.,State University Londrina | Korasaki V.,Federal University of Lavras | Catelli L.L.,Centro Universitario Filadelfia | Marcal V.V.M.,Centro Universitario Filadelfia | Nunes M.P.B.P.,Centro Universitario Filadelfia
Zoologia | Year: 2011

The study was conducted at the Mata dos Godoy State Park in Londrina, state of Paraná, Brazil. Baited pitfall traps were installed in the forest, in adjacent abandoned pasture land, and on the borders between these two environments. Every two weeks, from June 1999 to May 2000, the traps were placed for 24 hours and the material was collected every 12 hours. The 4687 specimens of Scarabaeinae collected belong to 13 genera and 27 species. The most frequent species were Onthophagus catharinensis (Paulian, 1936) (22.1%), Onthophagus hirculus (Mannerhein, 1829) (20.1%), Dichotomius mormon (Ljungh, 1799) (13.1%), Sulcophanaeus menelas (Laporte, 1840) (12.8%), and Eurystemus parallelus (Dalman, 1824) (10.6%). The other species collected amounted to less than 4.7%. The difference in numbers of specimens collected in the three environments was not significant (K (2.72) = 0.63, p = 0.729). The distribution patterns of species composition for the different environments (ANOSIM R = 0.29, p < 0.01) and activity periods (ANOSIM R = 0.18, p < 0.01) were different. Sulcophanaeus menelas, Canthidium aff. trinodosum, O. hirculus, and E. parallelus were the most abundant species, being predominantly diurnal; the first three species were more active on abandoned pasture. Most of the Dichothomius assifer (Eschscholtz, 1822) and D. mormon specimens were captured at night. The latter species was the most abundant in edges, whereas D. assifer, Scybalochantum aff. zischkai, and O. catharinensis can be considered bioindicators because they were either exclusively or highly predominant in the best preserved areas. A larger number of individuals was observed from December 1999 through March 2000, which is the time of the year when adults of Scarabaeinae normally emerge and fly, in the studied region. The highest diversity (88.89%) was observed in February. © 2011 Sociedade Brasileira de Zoologia.


Monica V.B.A.,State University Londrina | Daniela S.M.,Centro Universitario Filadelfia | De Reis Filho N.P.,Paulista University
Semina:Ciencias Agrarias | Year: 2015

Traumatic injuries to the vertebral column, spinal cord, and cauda equina nerve roots occur frequently in human and veterinary medicine and lead to devastating consequences. Complications include partial or complete loss of motor, sensory, and visceral functions, which are among the main causes of euthanasia in dogs. The present case report describes neurological functional recovery in two dogs that were treated surgically for severe spinal fracture and vertebral luxation. In the first case, a stray, mixed breed puppy was diagnosed with thoracolumbar syndrome and Schiff-Scherrington posture, as well as a T13 caudal epiphyseal fracture with 100% luxation between vertebrae T13 and L1; despite these injuries, the animal did show deep pain sensation in the pelvic limbs. Decompression through hemilaminectomy and spinal stabilization with vertebral body pins and bone cement were performed, and the treatment was supplemented with physiotherapy and acupuncture . In the second case, a mixed breed dog was diagnosed with a vertebral fracture and severe luxation between L6 and L7 after a vehicular trauma, but maintained nociception and perineal reflex. Surgical stabilization of the spine was performed using a modified dorsal segmental fixation technique Both patients showed significant recovery of neurological function. Complete luxation of the spinal canal observed radiographically does not mean a poor prognosis, and in some cases, motor, sensory, and visceral functions all have the potential for recovery. In the first case the determining factor for good prognosis was the presence of deep pain perception, and in the second case the prognosis was determined by the presence of sensitivity and anal sphincter tone during the initial neurological examination.


PubMed | Hospital Universitario Of Londrina, Centro Universitario Filadelfia, Secretaria Municipal de Saude and State University Londrina
Type: Journal Article | Journal: Epidemiologia e servicos de saude : revista do Sistema Unico de Saude do Brasil | Year: 2016

to demonstrate the experience of implanting toxoplasmosis notification during pregnancy and congenital toxoplasmosis in a Sentinel Unit (SU) and describe reported cases.this was a descriptive study of the implantation of a notification protocol using a specific notification form for suspected cases of toxoplasmosis in pregnant women and congenital toxoplasmosis in a reference center in Paran State, Brazil, from August 2013 to August 2014.all 64 suspected cases were notified and case investigation was completed by the SU Epidemiology Sector; 63 received prenatal care and 51 received treatment during pregnancy; 7 of the children being clinically monitored had confirmed diagnosis of congenital toxoplasmosis.implanting toxoplasmosis notification afforded epidemiological, clinical and diagnostic data on the disease that contributed to the assessment of the clinical evolution of children exposed to Toxoplasma gondii.


PubMed | Centro Universitario Filadelfia, Federal University of Mato Grosso do Sul, São Paulo State University and State University Londrina
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

The compounds 6-dimethylaminopurine (6-DMAP) and cyclohexamide (CHX) are currently used to stimulate the development of embryos produced by nuclear transfer in the production of cloned mammals with a great deal success. This study investigated the effects of 6-DMAP and CHX in vivo using biological assays to evaluate reproductive performance in females, teratogenesis, and mutagenesis. The results of this study demonstrated that the activating agents of oocyte cytoplasm, 6-DMAP and CHX, altered the reproductive performance of the experimental animals, as well as increased the rate malformations. In addition to these adverse effects, the administration of these compounds in pregnant females resulted in genotoxic and mutagenic toxicity, as determined by comet and micronucleus assays carried out in peripheral blood samples, respectively. Based on these findings and that alterations in DNA are important, morpho-functional teratogenesis and diminished embryonic viability, suggesting that 6-DMAP and CHX, which are utilized during the cloning of mammals, are responsible for the fact that embryos produced by nuclear transfer show low viability after implantation in utero or after birth because of congenital malformations and/or alterations in their DNA.


PubMed | Centro Universitario Filadelfia and Federal University of Mato Grosso do Sul
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

The present study investigated the effects of restricting protein and calories and supplementation of inulin, a fiber comprising a linear type of polydisperse carbohydrates composed primarily of fructil-fructose bonds (-(21), on the deficiency statuses of animals in which genomic lesion development and colorectal carcinogenesis had been induced. This experiment involved adult male Swiss mice (N = 11/group). The experimental groups were as follows: Negative Control (vehicle), Positive Control, 1,2-dimethylhydrazine (DMH), Inulin, and Associate. DMH, which promoted colorectal cancer, was administered intraperitoneally in 4 20-mg/kg body weight (bw) doses during a 2-week period; inulin was administered orally at a daily dose of 50 mg/kg bw. Each group was bifurcated; half of each group was fed a normal protein diet and the other half was fed a low-protein diet. The results indicated that a correlation existed between malnutrition and an increased frequency of genomic lesions but that malnutrition did not predispose animals to colorectal cancer development. Inulin exhibited genotoxic activity, which requires further investigation, and low anti-genotoxic activity. Moreover, inulin reduced the levels of intestinal carcinogenesis biomarkers in both malnourished and healthy animals. These data suggest that inulin holds therapeutic potential and is a strong candidate for inclusion among the functional foods used for cancer prevention in both properly nourished and malnourished individuals.

Loading Centro Universitario Filadelfia collaborators
Loading Centro Universitario Filadelfia collaborators