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Uberlândia, Brazil

Cardoso R.A.,Centro Universitario do Triangulo | Pires L.T.A.,Centro Universitario Moura Lacerda | Zucchi T.D.,University of Sao Paulo | Zucchi F.D.,University of Sao Paulo | Zucchi T.M.A.D.,University of Sao Paulo
Genetics and Molecular Research | Year: 2010

Some herbicides are suspected of promoting teratogenic, carcinogenic and mutagenic events. Detection of induced mitotic crossing-over has proven to be an indirect way of testing the carcinogenic properties of suspicious substances, because mitotic crossing-over is involved in the multistep process of carcinogenesis. We examined mitotic crossing-over induced by two commercial herbicides (diuron and trifluralin) in diploid strains of Aspergillus nidulans based on the homozygotization index. Low doses (2.5 μg/mL) of diuron were sufficient to increase the mean homozygotization index in 2.1 and 11.3 times for UT448// UT196 and Dp II-I//UT196, respectively, whereas the same dose of tri-fluralin increased this mean only 1.2 (UT448//UT196) and 3.5 (Dp II-I// UT196) times, respectively. The lower homozygotization index value found for trifluralin could be due to its interference with mitotic crossingover in eukaryotic cells. We concluded that the diploid Dp II-I//UT196 of A. nidulans is more sensitive to organic compounds than UT448//UT196; these compounds cause recombinational events at a greater frequency in the latter diploid. This system holds promise as an initial test for carcinogenicity of organic compounds, including herbicides. Source


Sanchez H.M.,University of Rio Verde | Sanchez E.G.M.,University of Rio Verde | Barauna M.A.,Centro Universitario do Triangulo | Canto R.S.T.,Federal University of Uberlandia
Acta Ortopedica Brasileira | Year: 2014

Objective: To compare the value of Q angle in different positions, in the external and internal rotations of lower limbs. Methods: This is a descriptive cross-sectional study. We have evaluated 62 volunteers, 32 women and 30 men in the following positions: supine positions with parallel feet, supine with abduction (external rotation of lower limbs), and standing position with parallel feet and with external rotation. All the participants were sedentary and without previous history of acute injury or complaints regarding lower limbs. In order to calculate the Q angle we used computerized biophotogrammetry through ALC image 2.1® program. Results: The results of the comparisons showed significant difference between the standing position with feet parallel and orthostatic positions with abductees feet on the left side for both genders (p = 0.000). We also found a significant difference between supine and standing position with abducted feet and with feet parallel on the left side (p = 0.046) in females. Conclusion: From these results, we can conclude that there are significant differences in the standing position with abducted feet and parallel to the left leg, and symmetry between the lower limbs independent of rotation of limbs in the supine posture. Source


Tambascio J.,University of Sao Paulo | De Souza L.T.,Centro Universitario do Triangulo | Lisboa R.M.,University of Sao Paulo | Passarelli R.D.C.V.,University of Sao Paulo | And 2 more authors.
Respiratory Medicine | Year: 2011

Background: The Flutter®VRP1 combines high frequency oscillation and positive expiratory pressure (PEP). Objective: To separately evaluate the effect of the Flutter®VRP1 components (high frequency oscillation and PEP) on mucus transportability in patients with bronchiectasis. Methods: Eighteen patients with bronchiectasis received sessions with the Flutter®VRP1 or PEP for 30 min daily in a randomized, crossover study. The treatment duration was four weeks with one of the therapies, one week of a "wash-out" period and followed by four more weeks with the other treatment. Weekly secretion samples were collected and evaluated for mucociliary relative transport velocity (RTV), displacement in a simulated cough machine (SCM) and contact angle measurement (CAM). For the proposed comparisons, a linear regression model was used with mixed effects with a significance level of 5%. Results: The Flutter®VRP1 treatment resulted in greater displacement in SCM and lower CAM when comparing results from the first (9.6 ± 3.4 cm and 29.4 ± 5.7°, respectively) and fourth weeks of treatment (12.44 ± 10.5 cm and 23.28 ± 6.2°, respectively; p < 0.05). There was no significant difference in the RTV between the treatment weeks for either the Flutter®VRP1 or PEP. Conclusion: The use of the Flutter®VRP1 for four weeks is capable of altering the respiratory secretion transport properties, and this alteration is related to the high frequency oscillation component. © 2011 Elsevier Ltd. All rights reserved. Source


Objective: To characterise and compare the in vitro transport properties of respiratory mucoid secretion in individuals with no lung disease and in stable patients with chronic obstructive pulmonary disease (COPD) and bronchiectasis. Methodology: Samples of mucus were collected, from 21 volunteers presenting no lung disease who had undergone surgery, from 10 patients presenting chronic COPD, and from 16 patients with bronchiectasis. Mucociliary transport (MCT), transport by cough (SCM), and contact angle (CAM) were evaluated. Results: MCT was found to be greater in healthy individuals (1.0 ± 0.19) than in COPD (0.91 ± 0.17) and bronchiectasis (0.76 ± 0.23) patients (p < 0.05), whereas SCM was greater in COPD patients (16.31 ± 7.35 cm) than in patients with bronchiectasis (12.16 ± 6.64 cm) and healthy individuals (10.50 ± 25.8 cm) (p < 0.05). No significant differences were observed between the groups regarding CAM. Conclusion: Mucus from healthy individuals allows better mucociliary transport compared to that from patients with lung diseases. However, the mucus from COPD patients allows a better transport by coughing, demonstrating that these individuals have adapted to a defence mechanism compared to patients with bronchiectasis, who have impairment in their ciliary and cough transport mechanisms. © 2012 Sociedade Portuguesa de Pneumologia. Source


Lima Afonso J.,Centro Universitario do Triangulo | Tambascio J.,University of Sao Paulo | Dutra De Souza H.C.,University of Sao Paulo | Jardimc J.R.,University of Sao Paulo | And 2 more authors.
Revista Portuguesa de Pneumologia | Year: 2013

Objective: To characterize and compare the in vitro transport properties of respiratory mucoid secretion in individuals with no lung disease and in stable patients with chronic obstructive pulmonary disease (COPD) and bronchiectasis. Methodology: Samples of mucus were collected from 21 volunteers presenting no lung disease who had undergone surgery, from 10 patients presenting chronic COPD, and from 16 patients with bronchiectasis. Mucociliary transport (MCT), transport by cough (SCM), and contact angle (CAM) were evaluated. Results: MCT was found to be greater in healthy individuals (1.0 ± 0.19) than in COPD (0.91 ±0.17) and bronchiectasis (0.76±0.23) patients (p<0.05), whereas SCM was greater in COPD patients (16.31 ±7.35cm) than in patients with bronchiectasis (12.16±6.64cm) and healthy individuals (10.50±25.8cm) (p<0.05). No significant differences were observed between the groups regarding CAM. Conclusion: Mucus from healthy individuals allows better mucociliary transport compared to that from patients with lung diseases. However, the mucus from COPD patients allows a better transport by coughing, demonstrating that these individuals have adapted to a defence mechanism compared to patients with bronchiectasis, who have impairment in their ciliary and cough transport mechanisms. © 2012 Sociedade Portuguesa de Pneumologia. Source

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