Garcia M.R.,Institute Salud Carlos III |
Ledgerwood L.,University of California at Davis |
Yang Y.,Mount Sinai School of Medicine |
Xu J.,Mount Sinai School of Medicine |
And 18 more authors.
Journal of Clinical Investigation | Year: 2010
One of the main unresolved questions in solid organ transplantation is how to establish indefinite graft survival that is free from long-term treatment with immunosuppressive drugs and chronic rejection (i.e., the establishment of tolerance). The failure to achieve this goal may be related to the difficulty in identifying the phenotype and function of the cell subsets that participate in the induction of tolerance. To address this issue, we investigated the suppressive roles of recipient myeloid cells that may be manipulated to induce tolerance to transplanted hearts in mice. Using depleting mAbs, clodronate-loaded liposomes, and transgenic mice specific for depletion of CD11c+, CD11b+, or CD115+ cells, we identified a tolerogenic role for CD11b+CD115+Gr1+ monocytes during the induction of tolerance by costimulatory blockade with CD40L-specific mAb. Early after transplantation, Gr1+ monocytes migrated from the bone marrow into the transplanted organ, where they prevented the initiation of adaptive immune responses that lead to allograft rejection and participated in the development of Tregs. Our results suggest that mobilization of bone marrow CD11b+CD115+Gr1+ monocytes under sterile inflammatory conditions mediates the induction of indefinite allograft survival. We propose that manipulating the common bone marrow monocyte progenitor could be a useful clinical therapeutic approach for inducing transplantation tolerance.
PubMed | Centro Universitario do Maranhao, Federal University of Minas Gerais, Laboratorio Of Enterotoxinas Of Alimentos and Federal University of Viçosa
Type: | Journal: Microbial pathogenesis | Year: 2016
Coagulase-negative staphylococci (CNS) represent one of the most prevalent microorganisms in nosocomial infections worldwide, nevertheless little is known about their pathogenicity features. Thus, our aim was to characterize virulence aspects of CNS isolated from patients with bloodstream infections assisted in hospitals of Belo Horizonte, MG, Brazil. Strains were identified using bioMrieuxVitek
Abreu A.G.,Federal University of Maranhão |
Marques S.G.,Federal University of Minas Gerais |
Monteiro-Neto V.,Federal University of Maranhão |
Monteiro-Neto V.,Centro Universitario do Maranhao |
Goncalves A.G.,Federal University of Maranhão
Brazilian Journal of Microbiology | Year: 2013
The number of ESBL-producing Enterobacteriaceae in community-acquired urinary tract infections worldwide is probably underestimated because of the technical difficulties encountered with their detection. In this study, out of 5,672 urine samples analyzed, 916 were positive for uropathogens, 472 of them being enterobacteria of which 7.6% produced β-lactamases. Analysis of the isolated from 36 patients showed a high level of antibiotic resistance, with 52.7% and 80.5% of isolates expressing blaTEM and blaCTX-M, respectively. © 2013, Sociedade Brasileira de Microbiologia.
Allometry of the babassu palm growing on a slash- and-burn agroecosystem of the eastern periphery of Amazonia [Allometria da palmeira babaçu em um agroecossistema de derruba-e- queima na periferia este da Amazônia]
Gehring C.,State University of Maranhão |
Zelarayan M.L.C.,State University of Maranhão |
Almeida R.B.,State University of Maranhão |
Moraes F.H.R.,Centro Universitario do Maranhao
Acta Amazonica | Year: 2011
Babassu (Attalea speciosa C.Martius, Arecaceae) is a palm with extraordinary socioeconomic and ecologic importance in large areas of tropical Brazil, especially in frequently burned and degraded landscapes. Nevertheless, surprisingly little is known about this keystone species. This paper investigates the allometry of babassu, in order to improve understanding on palm architecture and to provide researchers with an efficient tool for aboveground biomass estimation of juvenile and adult palms. Juvenile leaf biomass can be accurately predicted with the easily measurable minimum diameter of rachis at 30 cm extension. Adult palm biomass can be estimated based on woody stem height, a variable fairly easily measurable on-field. Leaf biomass of adult palms was highly variable, averaged 31.7% of aboveground biomass and can be estimated only indirectly through the relationships between wood: leaf-ratio and total aboveground biomass. Carbon contents varied little in the babassu palm, without size- or growth-stage related differences, suggesting the general applicability of values (42.5% C for stems, 39.8% C for leaves). As a consequence of the limited secondary diameter growth inherent to palms, stem diameter of adult palms is unrelated to palm height and biomass. Stem tapering decreases with increasing palm height. This is partially compensated by increasing wood density in near cylindrical stems. Nevertheless, maximum babassu palm height of about 30 meters appears to be dictated by mechanical stability constraints. All allometric relationships of babassu described in this study are not affected by vegetation stand age, indicating the general applicability of these relationships.
Miranda J.P.,University of Campinas |
Ricci-Lobao A.,Centro Universitario do Maranhao |
Rocha C.F.D.,State University of Rio de Janeiro
Zoologia | Year: 2010
We studied the structural habitat use and the thermal ecology of Gonatodes humeralis (Guichenot, 1855) in São Luís, Maranhão, Brazil, to examine intersexual differences in the use of perch features and to simultaneously analyze reciprocal differences on thermal ecology between the sexes. Gonatodes humeralis body temperature was strongly correlated with environmental temperatures (air and substrate), but air temperature had an additional effect on the males' body temperatures after removing the effect of the substrate temperature. Males and females differed significantly in perch height use above ground (males perched higher) but the sexes did not differ in the trunk perimeter used. Gonatodes humeralis tended to use the larger tree trunks available in its environment and selected trunks with deeper leaf litter at the base. It is hypothesized that choosing tree trunks with deeper leaf litter is a defensive behavior against predation. © 2010 Sociedade Brasileira de Zoologia.
Rosa R.,Federal University of Minas Gerais |
Costa E.A.,Federal University of Minas Gerais |
Marques R.E.,Federal University of Minas Gerais |
Oliveira T.S.,Federal University of Minas Gerais |
And 6 more authors.
PLoS Neglected Tropical Diseases | Year: 2013
St. Louis encephalitis virus (SLEV) is a causative agent of encephalitis in humans in the Western hemisphere. SLEV is a positive-sense RNA virus that belongs to the Flavivirus genus, which includes West Nile encephalitis virus, Japanese encephalitis virus, Dengue virus and other medically important viruses. Recently, we isolated a SLEV strain from the brain of a horse with neurological signs in the countryside of Minas Gerais, Brazil. The SLEV isolation was confirmed by reverse-transcription RT-PCR and sequencing of the E protein gene. Virus identity was also confirmed by indirect immunofluorescence using commercial antibodies against SLEV. To characterize this newly isolated strain in vivo, serial passages in newborn mice were performed and led to hemorrhagic manifestations associated with recruitment of inflammatory cells into the central nervous system of newborns. In summary this is the first isolation of SLEV from a horse with neurological signs in Brazil. © 2013 Rosa et al.
Brito L.M.O.,University of Sao Paulo |
Chein M.B.C.,Federal University of Rio de Janeiro |
Marinho S.C.,Federal University of Maranhão |
Duarte T.B.,Centro Universitario do Maranhao
Revista do Colegio Brasileiro de Cirurgioes | Year: 2011
Objective: to evaluate patients suffering from spinal cord injury Methods: A retrospective, cross-sectional study was conducted with 87 patients admitted to the university hospital of UFMA between January 2008 and June 2009. We assessed sex, age, compromised segment of the spine and cause of injury, subjecting these data to statistical analysis (chi-square test). Results: there was a significant prevalence of males (p <0.001), with 81.6% (71) cases, and age between 21 and 30 years of age (p <0.001), with 39.1% (34) of cases. The average age was 33.96 ± 13.56 years. The proportion of falls from height was significantly greater than the number of traffic (p <0.001) and motorcycle (p <0.001) accidents. The most compromised segment of the spine (p <0.001) was the thoracic (33), with 37.9% of cases. Traffic accidents appear in greater proportion among men (p = 0.014). The cervical spine was the most affected in males (p = 0.043). The thoracolumbar fractures were caused, to a greater extent, by falls from height (p = 0.003), whereas involvement of the thoracic spine was significantly higher (p = 0.016) in traffic accidents. Conclusion: The group at higher risk of injury to the spinal cord is the young adult male. Although there is a difference between the sexes when correlated traffic accidents, falling from height is the main cause in both sexes.
Inacio H.S.M.,Federal University of Minas Gerais |
Bomfim M.R.Q.,Centro Universitario do Maranhao |
Franca R.O.,Federal University of Minas Gerais |
Farias L.M.,Federal University of Minas Gerais |
And 3 more authors.
Chemotherapy | Year: 2014
Background: Pseudomonas aeruginosa commonly causes nosocomial bloodstream infections and the emergence of a variety of β-lactamases (BLs) is worrying. In 5 hospitals in Belo Horizonte, Brazil, the presence of phenotypes encoding BL genes was established and the genetic diversity of the P. aeruginosa strains recovered from bloodstream infections was analyzed. Materials and Methods: The isolates were investigated using a disk diffusion (DD) method and the Etest®, for encoding metallo-β-lactamases (MBLs), oxacillinases and cephalosporinases. Genes and genetic diversity were evaluated by random amplified polymorphic DNA (RAPD) genotyping and enterobacterial repetitive intergenic consensus (ERIC)-PCR. Results: Twelve strains (30%) were positive for MBLs by Etest and DD, 15 were cephalosporinase-positive and 87.5% were positive for blaSPM-1 and blaVIM-1. Twenty-three strains (57.5%) were grouped into profile A, 32.5% into profile B and 10% into profile C by RAPD genotyping. ERIC-PCR revealed a varying degree of similarity between strains, ranging from 45 to 100%. Conclusions: The results suggest distinct clonal populations in the 5 hospitals studied, indicating a potentially problematic epidemiological situation in Belo Horizonte, Brazil. © 2014 S. Karger AG, Basel.
Avelino F.,Autonomous University of Puebla |
Saldana Z.,University of Florida |
Islam S.,University of Florida |
Monteiro-Neto V.,Centro Universitario do Maranhao |
And 3 more authors.
International Journal of Medical Microbiology | Year: 2010
Enteroaggregative Escherichia coli (EAEC) have emerged as a significant worldwide cause of chronic diarrhea in the pediatric population and in HIV patients. The vast majority of EAEC strains do not produce the aggregative adherence fimbriae I-III (AAFs) so far reported and thus, what adherence factors are present in these strains remains unknown. Here, we investigated the prevalence of the chromosomal E. coli common pilus (ECP) genes and ECP production amongst 130 EAEC strains of diverse origin as well as the role of ECP in EAEC adherence. Through multiplex PCR analysis we found that 96% of EAEC strains contained the ecpA structural pilin gene whereas only 3.1% and 5.4% were positive for AAF fimbrial genes aggA or aafA, respectively. Among the ecpA+ strains, 63% produced ECP when adhering to cultured epithelial cells. An ecpA mutant derived from prototypic strain 042 (AAF/II+) was not altered in adherence suggesting that the AAF/II, and not ECP, plays a major role in this strain. In contrast, strain 278-1 (AAF-) deleted of the ecpA gene was significantly reduced in adherence to cultured epithelial cells. In all, these data indicate a potential role of ECP in adherence for EAEC strains lacking the known AAFs and that in association with other adhesive determinants, ECP may contribute to their survival and persistence within the host and in the environment. © 2010 Elsevier GmbH.
Nicolete R.,University of Sao Paulo |
Nicolete R.,Centro Universitario Do Maranhao |
Santos D.F.D.,University of Sao Paulo |
Faccioli L.H.,University of Sao Paulo
International Immunopharmacology | Year: 2011
Biodegradable micro/nanoparticles generated from PLGA have recently attracted attention due to their clinically proven biocompatibility, especially for immunization purposes. These polymeric particulate delivery systems are able to present antigens and activate both humoral and cellular responses. Many studies have discussed the ideal size of these particles in contributing to the generation of the different types of immune response. However, these studies do not demonstrate the effect of micro or nanoparticles, without any encapsulated bioactive, on phagocytic cells after the uptake process. In this context, the aim of this study was to analyze the in vitro inflammatory behavior of J774 murine macrophages after particles' uptake, since nano/microparticles per se can differently activate phagocytic cells, using or not appropriate receptors, inducing distinct inflammatory responses. An o/w emulsion solvent extraction-evaporation method was chosen to prepare the particles. We determined their diameters, zeta potential and morphology. Fluorescent particles' uptake by J774 murine "macrophage-like" cells was also analyzed. To evaluate the in vitro inflammatory profile of these cells after micro or nanoparticles' uptake, we conducted NF-κB translocation assay by confocal microscopy and also determined the pro-inflammatory cytokines production provoked by the particles. © 2011 Elsevier B.V. All rights reserved.