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Do Prado H.A.,Catholic University of Brasília | Bianchi Campos F.,Catholic University of Brasília | Ferneda E.,Catholic University of Brasília | Nunes Cornelio N.,Federal Bureau of Data Processing | And 2 more authors.
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) | Year: 2013

Since the arising of software engineering many efforts have been devoted to improve the software development process. More recently, software quality has received attention from researchers due to the importance that software has gained in supporting all levels of the organizations. New methods, techniques, and tools were created to increase the quality and productivity of the software development process. Approaches based on the practitioners' experience, for example, or on the analysis of the data generated during the development process, have been adopted. This paper follows the second path by applying data mining procedures to figure out variables from the development process that most affect the software quality. The premise is that the quality of decision making in management of software projects is closely related to information gathered during the development process. A case study is presented in which some regression models were built to explore this idea during the phases of testing, approval, and production. The results can be applied, mainly, to help the development managers in focusing those variables to improve the quality of the software as a final product. © 2013 Springer-Verlag.


Silva M.L.C.,Centro Universitario do Distrito Federal | Rangel E.R.,Centro Universitario do Distrito Federal | Lansac-Toha F.A.,State University of Maringá | Schwind L.T.F.,State University of Maringá | Joko C.Y.,Centro Universitario do Distrito Federal
Acta Scientiarum - Biological Sciences | Year: 2016

The pictorial key contributes to taxonomic analysis, as it lists the species found in a given environment, and points out the morphological characteristics that differs one specific taxon from the others. Arcellidae Ehrenberg, 1830 is one of the testate amoebae families with highest representativity in terms of richness and abundance, including three genera, that Arcella shows greater dispersion in different types of aquatic biotopes. The zooplankton community in the Paranoá Lake has been extensively studied over the decades. However, there are no studies on testate amoebae in this environment. The study aimed to develop a pictorial key to help in the identification of Arcella in the Paranoá Lake. Samples were taken with plankton net at 13 sites in the littoral zone of the lake. The elaboration of the key was based on morphological characteristics of the shell and pseudostoma of the individuals. There were identified 23 taxa, 16 species and seven subspecies. Two species with low incidence in Brazil were recorded (Arcella catinus and Arcella rota). The species richness was higher than the records published to date in Brazil, thus demonstrating the relevance of the data and the applicability of this pictorial key in other studies both in the Paranoá Lake, as in other regions. © 2016, Eduem - Editora da Universidade Estadual de Maringa. All rights reserved.


PubMed | Secretaria de Estado da Saude do Distrito Federal, Catholic University of Brasília, University of Sao Paulo, University of Brasilia and Centro Universitario do Distrito Federal
Type: Journal Article | Journal: Revista da Escola de Enfermagem da U S P | Year: 2016

Developing and validating an instrument to evaluate the playfulness of games in health education contexts.A methodological, exploratory and descriptive research, developed in two stages: 1. Application of an open questionnaire to 50 graduate students, with content analysis of the answers and calculation of Kappa coefficient for defining items; 2. Procedures for construction of scales, with content validation by judges and analysis of the consensus estimate by Content Validity Index(CVI).53 items regarding the restless character of the games in the dimensions of playfulness, the formative components of learning and the profiles of the players.Ludicity can be assessed by validated items related to the degree of involvement, immersion and reinvention of the subjects in the game along with the dynamics and playability of the game.


Moraes D.A.,University of Brasilia | Moraes D.A.,Centro Universitario Do Distrito Federal | Sibov T.T.,University of Sao Paulo | Pavon L.F.,University of Sao Paulo | And 8 more authors.
Stem Cell Research and Therapy | Year: 2016

Background: Mesenchymal stromal cells (MSCs) are multipotent progenitor cells used in several cell therapies. MSCs are characterized by the expression of CD73, CD90, and CD105 cell markers, and the absence of CD34, CD45, CD11a, CD19, and HLA-DR cell markers. CD90 is a glycoprotein present in the MSC membranes and also in adult cells and cancer stem cells. The role of CD90 in MSCs remains unknown. Here, we sought to analyse the role that CD90 plays in the characteristic properties of in vitro expanded human MSCs. Methods: We investigated the function of CD90 with regard to morphology, proliferation rate, suppression of T-cell proliferation, and osteogenic/adipogenic differentiation of MSCs by reducing the expression of this marker using CD90-target small hairpin RNA lentiviral vectors. Results: The present study shows that a reduction in CD90 expression enhances the osteogenic and adipogenic differentiation of MSCs in vitro and, unexpectedly, causes a decrease in CD44 and CD166 expression. Conclusion: Our study suggests that CD90 controls the differentiation of MSCs by acting as an obstacle in the pathway of differentiation commitment. This may be overcome in the presence of the correct differentiation stimuli, supporting the idea that CD90 level manipulation may lead to more efficient differentiation rates in vitro. © 2016 The Author(s).


Carvalho Rodrigues M.A.,Centro Universitario Do Distrito Federal | Santos N.A.G.D.,University of Sao Paulo | Da Silva Faria M.C.,Federal University of São Paulo | Rodrigues J.L.,Federal University of São Paulo | And 6 more authors.
Chemico-Biological Interactions | Year: 2016

Cisplatin (Cisp) is an effective antitumor drug; however, it causes severe nephrotoxicity. Minimization of renal toxicity is essential, but the interference of nephroprotective agents, particularly antioxidants, with the antitumor activity of cisplatin is a general concern. We have recently demonstrated that the anti-hypertensive and antioxidant drug carvedilol (CV) protects against the renal damage and increases the survival of tumor-bearing mice without impairing the tumor reduction by cisplatin. So far, reports on the antioxidant mechanism of CV are controversial and there are no data on the impact of CV on the antitumor mechanisms of cisplatin. Therefore, this study addresses the effect of CV on mechanisms underlying the tumor control by cisplatin. CV did not interfere with the biodistribution or the genotoxicity of cisplatin. We also addressed the antioxidant mechanisms of CV and demonstrated that it does not neutralize free radicals, but is an efficient chelator of ferrous ions that are relevant catalyzers in cisplatin nephrotoxicity. The present data suggest that oxidative damage and genotoxicity play different roles in the toxicity of cisplatin on kidneys and tumors and therefore, some antioxidants might be safe as chemoprotectors. Altogether, our studies provide consistent evidence of the beneficial effect of CV on animals treated with cisplatin and might encourage clinical trials. © 2016 Elsevier Ireland Ltd. All rights reserved.


Schalcher T.R.,Federal University of Pará | Borges R.S.,Federal University of Pará | Coleman M.D.,Aston University | Junior J.B.,Centro Universitario do Distrito Federal | And 5 more authors.
PLoS ONE | Year: 2014

This study aims to assess the oxidative stress in leprosy patients under multidrug therapy (MDT; dapsone, clofazimine and rifampicin), evaluating the nitric oxide (NO) concentration, catalase (CAT) and superoxide dismutase (SOD) activities, glutathione (GSH) levels, total antioxidant capacity, lipid peroxidation, and methemoglobin formation. For this, we analyzed 23 leprosy patients and 20 healthy individuals from the Amazon region, Brazil, aged between 20 and 45 years. Blood sampling enabled the evaluation of leprosy patients prior to starting multidrug therapy (called MDT 0) and until the third month of multidrug therapy (MDT 3). With regard to dapsone (DDS) plasma levels, we showed that there was no statistical difference in drug plasma levels between multibacillary (0.518±0.029 μg/mL) and paucibacillary (0.662±0.123 μg/mL) patients. The methemoglobin levels and numbers of Heinz bodies were significantly enhanced after the third MDTsupervised dose, but this treatment did not significantly change the lipid peroxidation and NO levels in these leprosy patients. In addition, CAT activity was significantly reduced in MDT-treated leprosy patients, while GSH content was increased in these patients. However, SOD and Trolox equivalent antioxidant capacity levels were similar in patients with and without treatment. These data suggest that MDT can reduce the activity of some antioxidant enzyme and influence ROS accumulation, which may induce hematological changes, such as methemoglobinemia in patients with leprosy. We also explored some redox mechanisms associated with DDS and its main oxidative metabolite DDS-NHOH and we explored the possible binding of DDS to the active site of CYP2C19 with the aid of molecular modeling software. © 2014 Schalcher et al.


de Lucca R.M.R.,Federal University of Mato Grosso | Batista Junior J.,Centro Universitario do Distrito Federal | Fontes C.J.F.,Federal University of Mato Grosso | Fontes C.J.F.,Hospital Universitario Julio Muller | And 2 more authors.
Environmental and Molecular Mutagenesis | Year: 2015

Lumefantrine (LF) is an aryl-amino alcohol antimalarial drug used in artemisinin-based combination therapies against malaria worldwide. In this study, we investigated the genotoxic effects of LF in human lymphocytes in vitro, and the potential noncovalent interaction of LF with DNA using a 3D DNA-docking model. The number of DNA breaks and the frequency of nuclear buds (NBUDS) was significantly increased (P<0.01 and P <0. 05, respectively) at LF concentrations of 60, 80, and 100 μg/mL (LF60, LF80, and LF100, respectively). Frequency (‰) of micronuclei (MN) formation also increased after LF treatments. However, this was only significant for LF100 (P = 0.01) and LF80 (P = 0.001). LF did not affect the frequency of nucleoplasmic bridges (NPBs) (P = 0.12) or the nuclear division index (NDI) (P = 0.32). Computational analysis suggests that LF may interact noncovalently with DNA via the DNA minor groove surface with a predicted binding affinity energy of -7.2 kcal/mol and showing a favorable shape complementary to this groove. Our results suggest that LF has clastogenic effects in human lymphocytes in vitro due to noncovalent interaction with the minor groove of DNA. Environ. Mol. Mutagen. 56:556-562, 2015. © 2015 Wiley Periodicals, Inc.


PubMed | Federal University of Pará, Federal University of Mato Grosso and Centro Universitario do Distrito Federal
Type: Journal Article | Journal: Environmental and molecular mutagenesis | Year: 2015

Lumefantrine (LF) is an aryl-amino alcohol antimalarial drug used in artemisinin-based combination therapies against malaria worldwide. In this study, we investigated the genotoxic effects of LF in human lymphocytes in vitro, and the potential noncovalent interaction of LF with DNA using a 3D DNA-docking model. The number of DNA breaks and the frequency of nuclear buds (NBUDS) was significantly increased (P < 0.01 and P < 0. 05, respectively) at LF concentrations of 60, 80, and 100 g/mL (LF60, LF80, and LF100, respectively). Frequency () of micronuclei (MN) formation also increased after LF treatments. However, this was only significant for LF100 (P = 0.01) and LF80 (P = 0.001). LF did not affect the frequency of nucleoplasmic bridges (NPBs) (P = 0.12) or the nuclear division index (NDI) (P = 0.32). Computational analysis suggests that LF may interact noncovalently with DNA via the DNA minor groove surface with a predicted binding affinity energy of -7.2 kcal/mol and showing a favorable shape complementary to this groove. Our results suggest that LF has clastogenic effects in human lymphocytes in vitro due to noncovalent interaction with the minor groove of DNA.


Alvarenga G.M.,Centro Universitario do Distrito Federal | da Silva E.B.,Centro Universitario do Distrito Federal | de Menezes M.E.B.,Centro Universitario do Distrito Federal | Del Sarto R.P.,Centro Universitario do Distrito Federal | And 2 more authors.
Biology and Medicine | Year: 2016

Since Dailey et al. first described the possible correlation between inflammation caused by autoimmune diseases and thyroid cancers in 1955, the majority of the events involving these two conditions have not been clearly elucidated, and this association is still very controversial in the literature. Chronic inflammation predisposes the organism to cell proliferation reactions, cytokines secretion and other phenomenas that influence rearrangements and mutations in thyroid follicular cells. Thus, is possible that in thyroid autoimmune phenotypes, the same mechanistic forces occur, mainly by the similarity of molecular events that affect both diseases. The large quantity of pro-inflammatory substances secreted within thyroid milieu in a chronic autoimmune condition, and the imbalance between anti and pro-apoptotic effectors, result in thyroid cells transformation, reducing thyroid hormones synthesis. Key important events regarding chronic inflammation momentum are those driving PTC carcinogenesis and its deregulation of the MAPK signaling pathway, causing rearrangements of RET/PTC, TRKA and mutation points in RAS and BRAF. In this review, we highlight the most relevant molecular events on thyroid disorders, giving an especial attention to mechanisms that drive molecular protagonists. © 2016 Alvarenga GM, et al.


PubMed | Centro Universitario do Distrito Federal, Catholic University of Brasília and Escola Superior de Ciencias da Saude
Type: Journal Article | Journal: Revista da Escola de Enfermagem da U S P | Year: 2016

Analyzing incidents reported in a public hospital in the Federal District, Brasilia, according to the characteristics and outcomes involving patients.A descriptive and retrospective study of incidents reported between January 2011 and September 2014.209 reported incidents were categorized as reportable occurrences (n = 22, 10.5%), near misses (n = 16, 7.7%); incident without injury (n = 4, 1.9%) and incident with injury (adverse events) (n = 167, 79.9%). The average age of patients was 44 years and the hospitalization time until the moment of the incident was on average 38.5 days. Nurses were the healthcare professionals who most reported the incidents (n = 55, 67%). No outcomes resulted in death.Incidents related to blood/hemoderivatives, medical devices/equipment, patient injuries and intravenous medication/fluids were the most frequent. Standardizing the reporting processes and enhancing participation by professionals in managing incidents is recommended.Analisar os incidentes notificados em um hospital pblico do Distrito Federal, segundo as caractersticas e os desfechos quando envolveram pacientes.Estudo descritivo e retrospectivo dos incidentes notificados entre janeiro de 2011 e setembro de 2014.Notificados 209 incidentes categorizados em ocorrncia comunicvel (n = 22, 10,5%), quase evento (n = 16, 7,7%), incidente sem dano (n = 4, 1,9%) e incidente com dano (eventos adversos) (n = 167, 79,9%). A idade mdia dos pacientes foi de 44 anos e o tempo da internao at o momento do incidente teve mdia de 38,5 dias. Os enfermeiros foram os que mais notificaram (n = 55, 67%). Nenhum desfecho resultou em morte.Os incidentes relacionados a sangue/hemoderivados, dispositivos/equipamento mdico, acidentes do doente e medicao/fluidos endovenosos foram os mais frequentes. Recomenda-se padronizar os processos de notificao e potencializar a participao dos profissionais no manejo dos incidentes.

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