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Trentini C.P.,State University of Maringá | Santos K.A.,West Parana State University | Antonio da Silva E.,West Parana State University | Garcia V.A.D.S.,Centro Universitario Da Fundacao Of Ensino Octavio Bastos Unifeob | And 3 more authors.
Journal of Supercritical Fluids | Year: 2017

In this study, the extraction of macauba pulp oil (MPO) using compressed propane as a solvent was investigated, and compared with conventional (Soxhlet) extraction. Extractions with propane were carried out in order to investigate the effects of temperature (333-373. K) and pressure (4-12. MPa) on the oil yield and the chemical composition of the products. The effects of temperature and pressure on the yield were negative and positive, respectively, with a maximum yield of 23.08. wt% being obtained at 333. K and 12. MPa. The use of propane allowed a fast extraction with a yield of ∼86% in the conventional extraction. The fatty acids composition showed a predominance (∼85%) of oleic and palmitic acids. The β-carotene and flavonoid contents were affected by the compressed-solvent extraction conditions and extraction method. Oil obtained using compressed-solvent extraction showed higher levels of phytosteroids and tocopherols and, consequently, a longer oxidation induction time. © 2017 Elsevier B.V.

Bertassoli B.M.,Federal University of Minas Gerais | Santos A.C.,University of Sao Paulo | De Oliveira F.D.,University of Sao Paulo | De Oliveira D.M.,University of Sao Paulo | And 2 more authors.
Ciencia Animal Brasileira | Year: 2013

Many species of opossums are created and used in laboratories, opening a wide field for studying and acquiring knowledge about the habits, diseases, diet and reproduction of these animals. The research aimed at describing the macroscopic and microscopical morphology of the trachea and larynx of opossums. In this study, five opossums (Didelphis sp.) wereused. The trachea and larynx of the opossums were extracted, measured and processed by histology routine and dyed with Hematoxiline eosine, Picrosirius and Massoń Trichrome. The larynx can be divided into: cricoid in a "V"-like shape, thyroid in a shield-like shape, arytenoid in shell-like shape, and epiglottis shaped like sheets, the first three structuresshowed hyaline cartilage, and the last structure showed elastic cartilage. The trachea showed a cylindrical form and it consists of 25 cartilaginous incomplete rings in a "C"-like shape, similar to amphibians, snakes, lizards and pigs; and different from domestic animals.

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