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Soto-Cerda B.J.,Agriaquaculture Nutritional Genomic Center | Inostroza-Blancheteau C.,Nucleo de Investigacion en Produccion Alimentaria | Inostroza-Blancheteau C.,Catholic University of Temuco | Mathias M.,Centro Regional Of Investigacion Carillanca | And 6 more authors.
Biologia Plantarum | Year: 2014

Aluminium toxicity in acid soils is the main limitation to crop production worldwide. In wheat (Triticum aestivum L.), the Al-activated malate transporter (TaALMT1) gene located on chromosome 4DL is associated with malate efflux and Al-tolerance. To introgress Al-tolerance from the breeding line CAR3911 into the high yielding Al-sensitive cultivar Kumpa-INIA, phenotypic and molecular characterizations of gene/QTL underlying Al-tolerance in CAR3911 followed by marker-assisted backcrossing (MAS-BC) were undertaken. Al-tolerant backcross (BC) lines were selected using the functional marker ALMT1-4 designed immediately upstream of the TaALMT1 coding region. Foreground and background selections using ALMT1-4 and microsatellite markers were conducted. Linkage and sequence analyses suggest that the TaALMT1 gene could underly the Al-tolerance in CAR3911, possessing the same promoter type (V) as the Al-tolerant genotypes Carazinho and ET8. The MAS-BC strategy allowed the selection of Al-tolerant lines with the smallest introgressed region (6 cM) on 4D and the highest recurrent parent genome (RPG) (98 %) covering 2 194 cM of the wheat genome. The homozygous BC3F2 line named Kumpa-INIA-TaALMT1 expressed a 3-fold higher Al-tolerance than its isogenic line Kumpa-INIA at 40 μM Al in the hydroponic solution, and similarly to CAR3911 and Carazinho. The MAS-BC strategy was successful for the introgression of the TaALMT1 gene into Kumpa-INIA in only three BC generations, shortening the breeding cycle to 24 months, which promises to increase wheat production and a greater yield stability in the acid soils of Southern Chile. © 2015, Springer Science+Business Media Dordrecht.

Piornos J.A.,Agriaquaculture Nutritional Genomic Center | Burgos-Diaz C.,Agriaquaculture Nutritional Genomic Center | Ogura T.,Agriaquaculture Nutritional Genomic Center | Morales E.,Agriaquaculture Nutritional Genomic Center | And 5 more authors.
Food Research International | Year: 2015

This study describes the isolation of proteins from the novel lupin variety AluProt-CGNA (Lupinus luteus) and the influence of pH and NaCl on their functional properties. AluProt-CGNA variety showed to have a great protein content in dehulled seeds (60.60g protein/100g, dry matter), which is higher than soybean and other lupin varieties. A lupin protein isolate (97.54g protein/100g) from AluProt-CGNA, LPIA, was prepared from lupin flour by alkali solubilization and isoelectric precipitation. The solubility profile of the LPIA was affected by pH, where the minimal values were observed at pH values close to its isoelectric point range (pH4-5). The highest values of water absorption capacity (1.71cm3H2O/g protein), oil absorption capacity (1.43g trapped oil/g protein), emulsifying capacity (61.94%), emulsion stability (96.43%), foaming capacity (114.29%), foam stability (65.69%) and least gelation concentration (20g/100cm3) were observed at pH values lower and higher than its isoelectric point. In the presence of 100mM of NaCl, their functional properties were improved. SDS-PAGE showed that LPIA mainly contained high molecular weight proteins (α and β-conglutin). These results are useful for increasing the utilization of this protein isolate as a potential functional ingredient in food industry. © 2015 Elsevier Ltd.

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