Rattmann Y.D.,University of Strasbourg |
Rattmann Y.D.,Centro Politcnico |
Anselm E.,University of Strasbourg |
Kim J.-H.,University of Strasbourg |
And 6 more authors.
Journal of Vascular Research | Year: 2012
Background/Aims: The consumption of polyphenol-rich food is associated with a decreased mortality from coronary diseases. This study examined whether a standardized hydroalcoholic extract of Dicksonia sellowiana (HEDS) triggered endothelium-dependent relaxations in porcine coronary artery rings and characterized the underlying mechanism. Methods: The phosphorylation level of Src, Akt and eNOS was assessed by Western blot analysis, the formation of reactive oxygen species by dihydroethidine staining and the level of eNOS Ser1177 phosphorylation by immunohistochemical staining in sections of coronary arteries. Results: HEDS-induced endothelium-dependent relaxations were strongly reduced by Nω-nitro-L-arginine, an eNOS inhibitor, and by its combination with charybdotoxin plus apamin, inhibitors of endothelium-derived hyperpolarizing factor-mediated responses. These relaxations were markedly reduced by MnTMPyP (a membrane-permeant mimetic of superoxide dismutase), polyethylene glycol catalase (PEG-catalase; a membrane-permeant analog of catalase), and by wortmannin (an inhibitor of PI3-kinase). HEDS-induced sustained phosphorylation of Akt and eNOS in endothelial cells was abolished by MnTMPyP, PEG-catalase and wortmannin. Oral administration of HEDS induced a significant decrease of mean arterial pressure in spontaneously hypertensive rats. Conclusion: These findings indicate that HEDS caused endothelium-dependent relaxations of coronary artery rings through the redox-sensitive activation of the endothelial PI3-kinase/Akt pathway leading to the subsequent activation of eNOS by phosphorylation. HEDS also has antihypertensive properties. © 2012 S. Karger AG, Basel.
Reinert B.L.,Materials Natura Institute Estudos Ambientais |
Belmonte-Lopes R.,Materials Natura Institute Estudos Ambientais |
Belmonte-Lopes R.,Centro Polytechnic |
Bornschein M.R.,Materials Natura Institute Estudos Ambientais |
And 7 more authors.
Wilson Journal of Ornithology | Year: 2012
We describe the nest and eggs of the Marsh Antwren (Stymphalornis acutirostris), a recently described species which is the only member of the Thamnophilidae restricted to marsh habitats. We conducted 1,560 hrs of nest searching in tidal marshes of southern Brazil and found 178 nests. All nests were of dry fibers, straws, and silk. Nineteen plant species were used for nest attachment. All nests had a clutch of two white eggs with several irregular brown spots scattered over the entire egg, concentrated at the middle or the largest pole. The strategy of nest attachment to vertical structures used by the Marsh Antwren was previously unknown in the Thamnophilidae. © 2012 by the Wilson Ornithological Society.
Belmonte-Lopes R.,Centro Politcnico |
Belmonte-Lopes R.,Materials NaturaInstitute Estudos Ambientais |
Maurcio G.N.,Grupo Especial de Estudo e Protecao do Ambiente Aquatico do Rio Grande do Sul |
Bornschein M.R.,Materials NaturaInstitute Estudos Ambientais |
Bornschein M.R.,Centro Politcnico
Wilson Journal of Ornithology | Year: 2011
We describe the nest and egg of the Black-headed Berryeater (Carpornis melanocephala), an Atlantic Forest endemic considered vulnerable to extinction. The nest was in a montane evergreen primary forest area in a tree fork 4.2 m above the ground. It was cup shaped and constructed mainly of leaves and stems, resembling a pile of aerial leaf litter. It held just one egg that was incubated solely by the female. The male was near the nest, and inspected it once while being observed. © 2011 by the Wilson Ornithological Society.