Centro Of Ricerca Per Lorticoltura Crea Ort

Pontecagnano Faiano, Italy

Centro Of Ricerca Per Lorticoltura Crea Ort

Pontecagnano Faiano, Italy
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Bombarely A.,Virginia Polytechnic Institute and State University | Moser M.,University of Bern | Amrad A.,University of Bern | Bao M.,Huazhong Agricultural University | And 53 more authors.
Nature Plants | Year: 2016

Petunia hybrida is a popular bedding plant that has a long history as a genetic model system. We report the whole-genome sequencing and assembly of inbred derivatives of its two wild parents, P. axillaris N and P. inflata S6. The assemblies include 91.3% and 90.2% coverage of their diploid genomes (1.4 Gb; 2n = 14) containing 32,928 and 36,697 protein-coding genes, respectively. The genomes reveal that the Petunia lineage has experienced at least two rounds of hexaploidization: the older gamma event, which is shared with most Eudicots, and a more recent Solanaceae event that is shared with tomato and other solanaceous species. Transcription factors involved in the shift from bee to moth pollination reside in particularly dynamic regions of the genome, which may have been key to the remarkable diversity of floral colour patterns and pollination systems. The high-quality genome sequences will enhance the value of Petunia as a model system for research on unique biological phenomena such as small RNAs, symbiosis, self-incompatibility and circadian rhythms. © 2016 Macmillan Publishers Limited.


PubMed | Boyce Thompson Institute for Plant Research, University of Cologne, Purdue University, University of Fribourg and 22 more.
Type: Journal Article | Journal: Nature plants | Year: 2016

Petunia hybrida is a popular bedding plant that has a long history as a genetic model system. We report the whole-genome sequencing and assembly of inbred derivatives of its two wild parents, P. axillaris N and P. inflata S6. The assemblies include 91.3% and 90.2% coverage of their diploid genomes (1.4 Gb; 2n=14) containing 32,928 and 36,697 protein-coding genes, respectively. The genomes reveal that the Petunia lineage has experienced at least two rounds of hexaploidization: the older gamma event, which is shared with most Eudicots, and a more recent Solanaceae event that is shared with tomato and other solanaceous species. Transcription factors involved in the shift from bee to moth pollination reside in particularly dynamic regions of the genome, which may have been key to the remarkable diversity of floral colour patterns and pollination systems. The high-quality genome sequences will enhance the value of Petunia as a model system for research on unique biological phenomena such as small RNAs, symbiosis, self-incompatibility and circadian rhythms.


PubMed | Centro Of Ricerca Per Lorticoltura Crea Ort, University of Salerno, CNR Institute of Neuroscience, University of Naples Federico II and ENEA
Type: | Journal: Gene | Year: 2016

Water-limiting conditions affect dramatically plant growth and development and, ultimately, yield of potato plants (Solanum tuberosum L.). Therefore, understanding the mechanisms underlying the response to water deficit is of paramount interest to obtain drought tolerant potato varieties. Herein, potato 10K cDNA array slides were used to profile transcriptomic changes of two potato cell populations under abrupt (shocked cells) or gradual exposure (adapted cells) to polyethylene glycol (PEG)-mediated water stress. Data analysis identified >1000 differentially expressed genes (DEGs) in our experimental conditions. Noteworthy, our microarray study also suggests that distinct gene networks underlie the cellular response to shock or gradual water stress. On the basis of our experimental findings, it is possible to speculate that DEGs identified in shocked cells participate in early protective and sensing mechanisms to environmental insults, while the genes whose expression was modulated in adapted cells are directly involved in the acquisition of a new cellular homeostasis to cope with water stress conditions. To validate microarray data obtained for potato cells, the expression analysis of 21 selected genes of interest was performed by Real-Time Quantitative Reverse Transcription PCR (qRT-PCR). Intriguingly, the expression levels of these transcripts in 4-week old potato plants exposed to long-term water-deficit. qRT-PCR analysis showed that several genes were regulated similarly in potato cells cultures and tissues exposed to drought, thus confirming the efficacy of our simple experimental system to capture important genes involved in osmotic stress response. Highlighting the differences in gene expression between shock-like and adaptive response, our findings could contribute to the discussion on the biological function of distinct gene networks involved in the response to abrupt and gradual adaptation to water deficit.


Taranto F.,Centro Of Ricerca Per Lorticoltura Crea Ort | Francese G.,Centro Of Ricerca Per Lorticoltura Crea Ort | Di Dato F.,Centro Of Ricerca Per Lorticoltura Crea Ort | D'Alessandro A.,Centro Of Ricerca Per Lorticoltura Crea Ort | And 5 more authors.
Journal of Agricultural and Food Chemistry | Year: 2016

Rocket salad (Diplotaxis spp., Eruca spp.) is a leafy vegetable rich in health-promoting compounds and widely consumed. In the present study, metabolic profiles of 40 rocket accessions mainly retrieved from gene banks were assessed. Seven glucosinolates (GLSs) and 15 flavonol compounds were detected across genotypes. Dimeric 4-mercaptobutyl-GLS and 4-(β-d-glucopyranosyldisulfanyl)butyl-GLS were the major components of the total glucosinolate content. Flavonols were different between genera, with the exception of isorhamnetin 3,4′-diglucoside. Morphoagronomic traits and color coordinates were also scored. Results showed a negative correlation between color and GLSs, indicating these last as responsible for the increase of the intensity of green and yellow pigments as well as for the darkness of the leaf, whereas agronomic traits showed positive correlation with GLSs. Genetic diversity was assessed using inter simple sequence repeat (ISSR) markers, allowing separation of the accessions on the basis of the species and elucidating the observations made by means of phenotypic data. © 2016 American Chemical Society.


Docimo T.,National Research Council Italy | Docimo T.,CNR Institute of Neuroscience | Francese G.,Centro Of Ricerca Per Lorticoltura Crea Ort | De Palma M.,National Research Council Italy | And 6 more authors.
Journal of Agricultural and Food Chemistry | Year: 2016

Color, taste, flavor, nutritional value, and shelf life are important factors determining quality and healthiness of food and vegetables. These factors are strongly affected by browning processes, occurring after fruit or vegetable cutting. Characterization of ten eggplant genotypes for chlorogenic acid (CGA) content, total phenols (TP), polyphenoloxidase (PPO) activity, and browning tendency corroborated a lack of significant correlations between biochemical factors and fruit flesh browning. Further in-depth molecular and biochemical analyses of two divergent eggplant genetic lines, AM199 (high browning) and AM086 (low browning), within 30 min from cutting, highlighted differences in the physiological mechanisms underlying the browning process. qRT-PCR analysis revealed distinct activation mechanisms of CGA biosynthetic and PPO genes in the two genetic lines. Metabolic data on CGA, sugars, and ascorbic acid contents confirmed that their different browning tendency matched with different metabolic responses to cutting. Our findings suggest that the complex mechanism of flesh browning in the two eggplant genetic lines might be mediated by multiple specific factors. © 2016 American Chemical Society.


Parisi M.,Centro Of Ricerca Per Lorticoltura Crea Ort | Di Dato F.,Centro Of Ricerca Per Lorticoltura Crea Ort | Ricci S.,Centro Of Ricerca Per Lorticoltura Crea Ort | Mennella G.,Centro Of Ricerca Per Lorticoltura Crea Ort | And 2 more authors.
Plant Genetic Resources: Characterisation and Utilisation | Year: 2015

Landraces are an important resource for crop breeding, due to their resilience and content of quality traits. However, genetic and phenotypic variability needs to be carefully characterized for proper direct and indirect use. In the present study, a multidisciplinary approach was carried out to assess the Italian sweet pepper landrace ‘Friariello’. A total of 18 traditional accessions were compared with five hybrids and two ecotypes with similar fruit typology. Genetic and morpho-agronomic characterization allowed us to distinguish five different group types of ‘Friariello’. Accessions showing two/three lobes at the blossom end of the fruit were found to be the most productive, whereas the genotypes showing one/two lobes at the blossom end were the most homogeneous. A total of 167 volatile organic compounds (VOCs) were identified in the collection analysed. Moreover, of the 37 targeted VOCs, 29 showed significant differences in content among the pepper genotypes studied. Of such VOCs related to main flavours described for pepper in the literature, ten were found to be the major determinants of variability among the derived ‘Friariello’ groups. A slightly negative, albeit not significant, correlation was observed between ascorbic acid (AsA) content and agronomic traits, suggesting a better quality for less productive accessions, but also the possibility to improve yield without significantly reducing the AsA levels. The approach used allowed us to define how the different typologies can be used for different breeding purposes, integrating the peculiar properties in order to establish a desirable landrace ideotype. Furthermore, valuable sources for improving quality traits in pepper breeding were identified. Copyright © NIAB 2015


PubMed | Centro Of Ricerca Per Lorticoltura Crea Ort, National Research Council Italy and Consiglio per la ricerca in agricoltura e lanalisi delleconomia agraria
Type: Journal Article | Journal: Journal of agricultural and food chemistry | Year: 2016

Color, taste, flavor, nutritional value, and shelf life are important factors determining quality and healthiness of food and vegetables. These factors are strongly affected by browning processes, occurring after fruit or vegetable cutting. Characterization of ten eggplant genotypes for chlorogenic acid (CGA) content, total phenols (TP), polyphenoloxidase (PPO) activity, and browning tendency corroborated a lack of significant correlations between biochemical factors and fruit flesh browning. Further in-depth molecular and biochemical analyses of two divergent eggplant genetic lines, AM199 (high browning) and AM086 (low browning), within 30 min from cutting, highlighted differences in the physiological mechanisms underlying the browning process. qRT-PCR analysis revealed distinct activation mechanisms of CGA biosynthetic and PPO genes in the two genetic lines. Metabolic data on CGA, sugars, and ascorbic acid contents confirmed that their different browning tendency matched with different metabolic responses to cutting. Our findings suggest that the complex mechanism of flesh browning in the two eggplant genetic lines might be mediated by multiple specific factors.

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