Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee

Acireale, Italy

Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee

Acireale, Italy
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Butelli E.,John Innes Center | Garcia-Lor A.,Instituto Valenciano Of Investigaciones Agrarias | Licciardello C.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee | Casas G.L.,University of Catania | And 11 more authors.
Plant Physiology | Year: 2017

Mandarin (Citrus reticulata), citron (Citrus medica), and pummelo (Citrus maxima) are important species of the genus Citrus and parents of the interspecific hybrids that constitute the most familiar commercial varieties of Citrus: sweet orange, sour orange, clementine, lemon, lime, and grapefruit. Citron produces anthocyanins in its young leaves and flowers, as do species in genera closely related to Citrus, but mandarins do not, and pummelo varieties that produce anthocyanins have not been reported. We investigated the activity of the Ruby gene, which encodes a MYB transcription factor controlling anthocyanin biosynthesis, in different accessions of a range of Citrus species and in domesticated cultivars. A white mutant of lemon lacks functional alleles of Ruby, demonstrating that Ruby plays an essential role in anthocyanin production in Citrus. Almost all the natural variation in pigmentation by anthocyanins in Citrus species can be explained by differences in activity of the Ruby gene, caused by point mutations and deletions and insertions of transposable elements. Comparison of the allelic constitution of Ruby in different species and cultivars also helps to clarify many of the taxonomic relationships in different species of Citrus, confirms the derivation of commercial varieties during domestication, elucidates the relationships within the subgenus Papeda, and allows a new genetic classification of mandarins. © 2017 American Society of Plant Biologists. All rights reserved.


Butelli E.,John Innes Center | Licciardello C.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee | Zhang Y.,John Innes Center | Liu J.,Sichuan Academy of Agricultural science | And 4 more authors.
Plant Cell | Year: 2012

Traditionally, Sicilian blood oranges (Citrus sinensis) have been associated with cardiovascular health, and consumption has been shown to prevent obesity in mice fed a high-fat diet. Despite increasing consumer interest in these healthpromoting attributes, production of blood oranges remains unreliable due largely to a dependency on cold for full color formation. We show that Sicilian blood orange arose by insertion of a Copia-like retrotransposon adjacent to a gene encoding Ruby, a MYB transcriptional activator of anthocyanin production. The retrotransposon controls Ruby expression, and cold dependency reflects the induction of the retroelement by stress. A blood orange of Chinese origin results from an independent insertion of a similar retrotransposon, and color formation in its fruit is also cold dependent. Our results suggest that transposition and recombination of retroelements are likely important sources of variation in Citrus. © 2012 American Society of Plant Biologists. All rights reserved.


Laino P.,Genomics Research Center | Russo M.P.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee | Guardo M.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee | Reforgiato-Recupero G.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee | And 3 more authors.
Physiologia Plantarum | Year: 2016

Citrus tristeza virus (CTV) is the causal agent of various diseases with dramatic effects on citrus crops worldwide. Most Citrus species, grown on their own roots, are symptomless hosts for many CTV isolates. However, depending on different scion-rootstock combination, CTV infection should result in distinct syndromes, being 'tristeza' the more severe one, leading to a complete decline of the susceptible plants in a few weeks. Transcriptomic analyses revealed several genes involved either in defense response, or systemic acquired resistance, as well as transcription factors and components of the phosphorylation cascades, to be differentially regulated during CTV infection in Citrus aurantifolia species. To date little is known about the molecular mechanism of this host-pathogen interaction, and about the rootstock effect on citrus response to CTV infection. In this work, the response to CTV infection has been investigated in tolerant and susceptible scion-rootstock combinations by two-dimensional gel electrophoresis (2DE). A total of 125 protein spots have been found to be differently accumulated and/or phosphorylated between the two rootstock combinations. Downregulation in tolerant plants upon CTV infection was detected for proteins involved in reactive oxygen species (ROS) scavenging and defense response, suggesting a probable acclimation response able to minimize the systemic effects of virus infection. Some of these proteins resulted to be modulated also in absence of virus infection, revealing a rootstock effect on scion proteome modulation. Moreover, the phospho-modulation of proteins involved in ROS scavenging and defense response, further supports their involvement either in scion-rootstock crosstalk or in the establishment of tolerance/susceptibility to CTV infection. © 2016 Scandinavian Plant Physiology Society.


Cocuzza G.E.M.,University of Catania | Mazzeo G.,University of Catania | Russo A.,University of Catania | Giudice V.L.,Agronomist | Bella S.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee
Phytoparasitica | Year: 2016

Here we review the arthropod pests most damaging to pomegranate cultivation in the main production regions of the Circum-Mediterranean area, and discuss the eventual phytosanitary risks linked to some newly introduced species in this geographical area. The arthropod species mentioned here are based on their occurrence and economic importance. These are either mites (Tenuipalpidae) or insects belonging to Hemiptera (Flatidae, Aphididae, Aleyrodidae, Coccidae, Diaspididae, and Pseudococcidae), Lepidoptera (Cossidae, Lycaenidae, Erebidae, and Pyralidae), Diptera (Tephritidae), and Coleoptera (Bostrichidae and Nitidulidae). In the Circum-Mediterranean area, the major pests are Aphis punicae, A. gossypii, Planococcus spp., Zeuzera pyrina, Apomyelois ceratoniae, Deudorix livia and Cryptoblades gnidiella. A nod is also given to those species normally considered as minor pests that, in some environment and under specific conditions, can be of major concern. Particular attention should be paid toward avoiding the introduction of two species that could become a serious problem for pomegranate that are Thaumatotibia leucotreta and Deudorix isocrates. The main morphological and biological characteristics of each pomegranate pest species and the tools available in their integrated and organic control are briefly presented. © 2016 Springer Science+Business Media Dordrecht


Caruso P.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee | Baldoni E.,CNR Institute of Agricultural Biology and Biotechnology | Mattana M.,CNR Institute of Agricultural Biology and Biotechnology | Pietro Paolo D.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee | And 6 more authors.
Plant Cell, Tissue and Organ Culture | Year: 2012

Oxygen deficit, which occurs in flooded or poorly drained soils, can limit plant growth and development. Low-oxygen environmental conditions also limit the distribution of many woody plants, such as citrus trees, which are considered flood-sensitive crops, although tolerance to this stress varies among genotypes and rootstocks. In this study, the rice transcription factor Mybleu was inserted into the pGA470 plant cloning vector and transferred into the epicotyl explants of the Carrizo citrange rootstock (Citrus sinensis × Poncirus trifoliata) using Agrobacterium tumefaciens-mediated transformation. The transgenic lines were confirmed for the presence and expression of the transgene, and physiological, biochemical and molecular parameters were evaluated for adaptation to hypoxic and anoxic stress conditions. The ectopic expression of Mybleu increased tolerance to oxygen deprivation in the transgenic lines, contributing to increased viability under this stress condition. This improved tolerance correlates with, and may depend on, the induction of genes and the activation of enzymes from various fermentation and carbohydrate metabolic pathways, antioxidant systems and nonsymbiotic haemoglobin-nitric oxide homeostasis mechanisms. Together, our data suggest a key role for Mybleu in coordinating the multifaceted plant response to low oxygen stress and the conservation of Mybleu-regulated pathways among species. © 2011 Springer Science+Business Media B.V.


Fabroni S.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee | Ballistreri G.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee | Amenta M.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee | Romeo F.V.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee | Rapisarda P.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee
Journal of the Science of Food and Agriculture | Year: 2016

BACKGROUND: The phytotherapic treatment of overweight and/or moderate obesity is growing widely, thus there is a great interest towards the phenolic compounds of fruits and vegetables which may inhibit pancreatic lipase enzyme. In this study, we report the chemical composition and in vitro pancreatic lipase inhibitory activity of 13 freeze-dried anthocyanin-containing extracts of different Mediterranean plants: fruits (blood orange, pomegranate, blackberry, mulberry and sumac), citrus by-products (blood orange peel), citrus vegetative tissues (young lemon shoots); vegetables (red cabbage and violet cauliflower), legume seeds (black bean), cereals (black rice), and cereal processing by-products (black rice hull). Total phenols and anthocyanins were determined. Individual anthocyanins were identified by UHPLC-PDA-ESI/MSn. RESULTS: Results revealed a wide variation in the distribution of anthocyanin compounds. Blood orange and pomegranate juice extracts had the highest total anthocyanin content and exhibited the strongest inhibition of pancreatic lipase in vitro. CONCLUSION: Inhibitory activity was positively correlated with anthocyanin content. In appropriate formulations, anthocyanin-containing extracts could find a use as anti-obesity agents. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry


Cocuzza G.E.M.,University of Catania | Silvestro S.D.,Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee | Giordano R.,University of Puerto Rico at San Juan | Rapisarda C.,University of Catania
ZooKeys | Year: 2015

The discrimination of species in the genus Anuraphis is particularly difficult due to the overlap of morphological characters. In this study, we used the 5’ (barcode) and 3’ regions of cytochrome oxidase I (COI) to test their utility in the identification of species in this genus as well as closely related species. Both regions were useful to discriminate all the species tested. However the non-barcode 3’ region resulted in higher resolution and support for species relationships when the data were analyzed using both Maximum Likelihood and MrBayes. We propose the development of an integrated database that encompasses morphological, molecular, life-cycle, host plant and bibliographic information to facilitate and increase the accuracy of aphid identification. © Giuseppe E. Massimino Cocuzza et al.


PubMed | University of Catania, Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee and University of Puerto Rico at San Juan
Type: | Journal: ZooKeys | Year: 2015

The discrimination of species in the genus Anuraphis is particularly difficult due to the overlap of morphological characters. In this study, we used the 5 (barcode) and 3 regions of cytochrome oxidase I (COI) to test their utility in the identification of species in this genus as well as closely related species. Both regions were useful to discriminate all the species tested. However the non-barcode 3 region resulted in higher resolution and support for species relationships when the data were analyzed using both Maximum Likelihood and MrBayes. We propose the development of an integrated database that encompasses morphological, molecular, life-cycle, host plant and bibliographic information to facilitate and increase the accuracy of aphid identification.


PubMed | Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee
Type: Journal Article | Journal: Journal of the science of food and agriculture | Year: 2016

The phytotherapic treatment of overweight and/or moderate obesity is growing widely, thus there is a great interest towards the phenolic compounds of fruits and vegetables which may inhibit pancreatic lipase enzyme. In this study, we report the chemical composition and in vitro pancreatic lipase inhibitory activity of 13 freeze-dried anthocyanin-containing extracts of different Mediterranean plants: fruits (blood orange, pomegranate, blackberry, mulberry and sumac), citrus by-products (blood orange peel), citrus vegetative tissues (young lemon shoots); vegetables (red cabbage and violet cauliflower), legume seeds (black bean), cereals (black rice), and cereal processing by-products (black rice hull). Total phenols and anthocyanins were determined. Individual anthocyanins were identified by UHPLC-PDA-ESI/MSResults revealed a wide variation in the distribution of anthocyanin compounds. Blood orange and pomegranate juice extracts had the highest total anthocyanin content and exhibited the strongest inhibition of pancreatic lipase in vitro.Inhibitory activity was positively correlated with anthocyanin content. In appropriate formulations, anthocyanin-containing extracts could find a use as anti-obesity agents. 2016 Society of Chemical Industry.


PubMed | Centro Of Ricerca Per Lagrumicoltura E Le Colture Mediterranee, Genomics Research Center and Rice Research Unit
Type: Journal Article | Journal: Physiologia plantarum | Year: 2016

Citrus tristeza virus (CTV) is the causal agent of various diseases with dramatic effects on citrus crops worldwide. Most Citrus species, grown on their own roots, are symptomless hosts for many CTV isolates. However, depending on different scion-rootstock combination, CTV infection should result in distinct syndromes, being tristeza the more severe one, leading to a complete decline of the susceptible plants in a few weeks. Transcriptomic analyses revealed several genes involved either in defense response, or systemic acquired resistance, as well as transcription factors and components of the phosphorylation cascades, to be differentially regulated during CTV infection in Citrus aurantifolia species. To date little is known about the molecular mechanism of this host-pathogen interaction, and about the rootstock effect on citrus response to CTV infection. In this work, the response to CTV infection has been investigated in tolerant and susceptible scion-rootstock combinations by two-dimensional gel electrophoresis (2DE). A total of 125 protein spots have been found to be differently accumulated and/or phosphorylated between the two rootstock combinations. Downregulation in tolerant plants upon CTV infection was detected for proteins involved in reactive oxygen species (ROS) scavenging and defense response, suggesting a probable acclimation response able to minimize the systemic effects of virus infection. Some of these proteins resulted to be modulated also in absence of virus infection, revealing a rootstock effect on scion proteome modulation. Moreover, the phospho-modulation of proteins involved in ROS scavenging and defense response, further supports their involvement either in scion-rootstock crosstalk or in the establishment of tolerance/susceptibility to CTV infection.

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