National survey on the use of commercial assays employing method principles in agreement with IFCC reference methods for the measurement of catalytic activity of serum enzymes [Inchiesta nazionale sull'impiego di metodi commerciali basati sul principio analitico raccomandato dall'IFCC per la determinazione dell'attività catalitica degli enzimi nel siero]
Cattozzo G.,Azienda Ospedaliero |
Borsotti M.,Azienda Ospedaliero |
Carobene A.,Diagnostica e Ricerca San Raffaele SpA |
Incorvaia L.,Azienda Ospedaliera Policlinico S. Orsola Malpighi |
And 2 more authors.
Biochimica Clinica | Year: 2010
Method-dependent variability of enzyme catalytic activities jeopardizes the use of uniform clinical decision limits and confuses the understanding of results observed in patient sera and in control materials as well. Results by different methods may be harmonized making the results they produce traceable to a selected higher metrological level: however, it may be easier to achieve result traceability by widespread use of one selected analytical principle comparable with a reference, i.e. the IFCC, reference procedure. The aim of this work was to check the spread in Italian laboratories of assays based on IFCC reference method principles for measurement of alanine transaminase (ALT), α-amylase (AMY), aspartate transaminase (AST), creatine kinase (CK), γ-glutamiltransferase (GGT), and lactate dehydrogenase (LDH) activities. By distributing a questionnaire to organizers of five national EQAS, we collected information about the enzyme analytical methodologies selected in about 1400 laboratories. We found that 86%, 44%, 15% and 10% of laboratories adopted the relevant IFCC principle for CK, AMY, LDH and AST/ALT measurements, respectively. For GGT we observed that the IFCC principle was adopted by 56% of laboratories participating in the two EQAS distinguishing a specific group for the IFCC method. However, 83% of the laboratories participating in EQAS used L-γ-glutamyl-3-carboxy-4-nitroanilide as substrate.
Fogazzi G.B.,Research Laboratory on Urine |
Secchiero S.,Centro Of Ricerca Biomedica |
Consonni D.,Unita Operativa di Epidemiologia |
Sciacovelli L.,Centro Of Ricerca Biomedica |
And 5 more authors.
Clinica Chimica Acta | Year: 2010
Background: EQA programs on urinary sediment are rare. We describe an EQA Italian program which started in 2001 and involves today more than 300 laboratories. Methods: The program, which started with a questionnaire about the methodological aspects on urinary sediment, includes today four surveys per year. These ask the participants the identification and clinical associations of urinary sediment particles shown by colour images (surveys 1 and 3) and the diagnosis of clinical cases presented by both images and a short clinical history (surveys 2 and 4). The results of each survey are then scored and commented. Results: Questionnaire (participants = 287): most methodological aspects were not dealt with properly. Identification: cells, lipids, casts and some contaminants were poorly known. However, when 27 particles were presented for the second time and 16 particles for the third time, the correct identification rate for most of them increased significantly. Clinical associations (No presented = 16): a correct answer was indicated by ≥. 84% of participants for all particles but one. Clinical cases (No presented = 4): lowest correct identification for urine contamination from genital secretion (77.3%), highest for ureteric stone (94.4%). Conclusions: Our program shows that EQA programs are both useful and needed. © 2010 Elsevier B.V.