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Ferrantelli V.,Centro Of Referenza Nazionale Per Le Anisakiasi Crena | Cicero A.,Centro Of Referenza Nazionale Per Le Anisakiasi Crena | Costa A.,Centro Of Referenza Nazionale Per Le Anisakiasi Crena | Alongi A.,Centro Of Referenza Nazionale Per Le Anisakiasi Crena | And 3 more authors.
Italian Journal of Food Safety | Year: 2014

One of the parasite diseases associated with the consumption of raw fish that occurs with some frequency is the anisakiasis, a human disease caused by the accidental ingestion of larval nematodes of the genus Anisakis, family Anisakidae. At the National Reference Centre for Anisakiasis (C.Re.N.A.) from October 2012 to February 2013, a number of 231 bony fish (Trichiuridae, Engraulidae, Scombridae and Clupeidae) were received from different fishing sites in Sicily. Anisakis pegreffii is the main species detected in fish, as identified by molecular analysis based on polymerase chain reaction-restriction fragment length polymorphism, while Anisakis simplex sensu stricto was found only in Scomber scombrus caught in the Mediterranean Sea (Fishing Areas 37), in the Spanish coast (Fishing Areas 37) and in the Atlantic Ocean (Fishing Areas 34). Larvae of the genus Pseudoterranova were found only in fish caught in the Norwegian Sea. Source


Cammilleri G.,Centro Of Referenza Nazionale Per Le Anisakiasi Crena | Chetta M.,Centro Of Referenza Nazionale Per Le Anisakiasi Crena | Costa A.,Centro Of Referenza Nazionale Per Le Anisakiasi Crena | Graci S.,Centro Of Referenza Nazionale Per Le Anisakiasi Crena | And 8 more authors.
Acta Parasitologica | Year: 2016

Anisakis and other parasites belonging to the Anisakidae family are organisms of interest for human health, because of their high zoonotic potential. Parasites belonging to this family can cause Anisakiasis, a parasitological disease caused by the ingestion of raw, infested fish products. Furthermore, evidence from the EFSA (European Food Safety Authority; EFSA 2010) has highlighted the allergological potential of nematodes belonging to the Anisakis genre. The detection and identification of Anisakidae larvae in fish products requires an initial visual inspection of the fish sample, as well as other techniques such as candling, UV illumination and artificial digestion. The digestion method consists of the simulation of digestive mechanics, which is made possible by the utilization of HCl and pepsin, according to EC Regulation 2075/2005. In this study, a new Anisakidae larvae detection method using a mechanical digestion system called Trichineasy® was developed. A total of 142 fish samples, belonging to 14 different species, were examined to validate the method. A reaction mixture with 100 g of sample, 10 g of pepsin (1:10000 NF) and 50 ml of 10% HCl at 36 ± 1°C for 20 minutes was evaluated to be the best condition for the digestion of fish samples. These parameters have also allowed the detection of viable larvae after digestion. The results confirm this instrumentation as a valuable and safe tool for the detection of Anisakidae larvae in fishery products. © W. Stefański Institute of Parasitology, PAS 2016. Source

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