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Omes C.,Centro Of Procreazione Medicalmente Assistita | Fassina L.,University of Pavia | Magenes G.,University of Pavia | Ogliari D.,University of Pavia | And 2 more authors.
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS | Year: 2013

Low-Intensity Pulsed Ultrasound Stimulus (LIPUS) accelerates the bone fracture healing in animal models and in clinical studies. In this work, according to the literature, we have chosen the mesenchymal stem cells (MSCs) as precursors of bony tissue, in particular the MSCs derived from the human ovarian follicular liquid (FL), and we have investigated the effects of ultrasounds on their proliferation. We tested two different durations of ultrasound stimulus (2 and 5 min) and compared these data to the control without ultrasound treatment. To quantify the proliferation of these putative MSCs, we used the BrdU incorporation assay: in comparison with the control, the results showed that 5 min of ultrasound stimulus significantly increased the percentage number of cells in intensive proliferative activity; on the other hand, there was no significant difference using 2 min of stimulation, hypothetically because the transmitted energy was not sufficient to stimulate the cells and to consequently enhance their proliferation. In conclusion, the effects of LIPUS on putative MSCs derived from ovarian follicular liquid show potential developments in biotech or medical applications. © 2013 IEEE. Source


Fassina L.,University of Pavia | Visai L.,University of Pavia | Magenes G.,University of Pavia | Schelfhout J.,Ghent University | And 10 more authors.
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS | Year: 2013

In the present study, gelatin-based cryogels have been seeded with human SAOS-2 osteoblasts. In order to overcome the drawbacks associated with in vitro culture systems, such as limited diffusion and inhomogeneous cell-matrix distribution, this work describes the application of ultrasounds (average power, 149 mW; frequency, 1.5 MHz) to physically enhance the cell culture in vitro. The results indicate that the physical stimulation of cell-seeded gelatin-based cryogels upregulates the bone matrix production. © 2013 IEEE. Source


Coccia M.E.,University of Florence | Coccia M.E.,Centro Of Procreazione Medicalmente Assistita | Rizzello F.,Centro Of Procreazione Medicalmente Assistita | Rizzello F.,University of Rome La Sapienza | And 8 more authors.
Reproductive BioMedicine Online | Year: 2014

This study investigated the relationships between ovarian endometrioma size, ovarian responsiveness and the number of retrieved oocytes following ovarian stimulation. A prospective study was conducted in a public clinical assisted reproduction centre. A total of 64 infertile women with monolateral endometriomas undergoing IVF or intracytoplasmic sperm injection were included in the study. The total number of follicles, number of follicles 16 mm and number of oocytes retrieved of ovaries containing endometrioma and normal ovaries were compared. Multivariate linear regression was used to assess whether number of follicles and collected oocytes varied by endometrioma size, age, basal FSH concentration. Significantly lower numbers of follicles 16 mm (P = 0.024) and oocytes retrieved (P = 0.001) in the ovaries containing endometrioma were observed. In patients with endometriomas 30 mm, endometrioma size was the most influential contributor to the total number of follicles and oocytes retrieved. Ovarian endometriomas result in reduced response to ovarian stimulation, compared with the response of the contralateral normal ovary in the same individual. In case of endometriomas <30 mm, basal FSH concentration remains the most important prognostic factor for oocyte retrieval. © 2014 Reproductive Healthcare Ltd. © 2014 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved. Source


Omes C.,Centro Of Procreazione Medicalmente Assistita | Fassina L.,University of Pavia | Van Vlierberghe S.,Ghent University | Magenes G.,Centro Of Procreazione Medicalmente Assistita | And 4 more authors.
Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS | Year: 2013

Significant research efforts have been undertaken in the last decade to develop specific cell-based therapies and, in particular, adult multipotent mesenchymal stem cells (MSCs) hold great promise toward such regenerative strategies. Bio-materials have been widely used in reconstructive bone surgery to heal critical-size bone defects due to trauma, tumor resection, and tissue degeneration. In particular, gelatin cryogel scaffolds are promising new biomaterials owing to their biocompatibility. There is an increasing demand for MSC-based regenerative approaches in the musculoskeletal system. Combining stem cells with biomaterial scaffolds provides a promising strategy for tissue engineering. Our previous studies showed the possibility to obtain MSCs from the human ovarian follicular liquid (FL) that is usually wasted during in vitro fertilization (IVF). In this study, we tested the ability of these FL cells to grow on gelatin cryogel in comparison with MSCs derived from human bone marrow. Samples and controls were analyzed with confocal and scanning electron microscopes. Results demonstrated that FL cells could grow on the biomaterial not only on the top but also in the layers below till 60 μm of deepness. Data suggested that the observed cells were mesenchymal since positive for vimentin and CD-44, typical MSC markers. Successful growth of putative MSCs derived from follicular liquid on 3D gelatin cryogel opens potential developments in biotech and medical applications. © 2013 IEEE. Source

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