Centro Multidisciplinario Of Estudios En Biotecnologia

Mexico City, Mexico

Centro Multidisciplinario Of Estudios En Biotecnologia

Mexico City, Mexico
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Zamora-Aviles N.,Institute Investigaciones Agropecuarias y Forestales | Murillo R.,Institute Agrobiotecnologia | Murillo R.,Public University of Navarra | Lasa R.,Institute Ecologia AC | And 6 more authors.
Journal of Economic Entomology | Year: 2017

This study describes four multiple nucleocapsid nucleopolyhedrovirus isolates recovered from infected larvae of beet armyworm, Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae), on crops in two different geographical regions of Mexico. Molecular and biological characterization was compared with characterized S. exigua multiple nucleopolyhedrovirus (SeMNPV) isolates from the United States (SeUS1 and SeUS2) and Spain (SeSP2). Restriction endonuclease analysis of viral DNA confirmed that all Mexican isolates were SeMNPV isolates, but molecular differences between the Mexican and the reference isolates were detected using PCR combined with restriction fragment length polymorphism (RFLP). Amplification of the variable region V01 combined with RFLP distinguished the two Mexican isolates, SeSLP6 and SeSIN6. BglII digestions showed that the majority of the isolates contained submolar bands, indicating the presence of genetic heterogeneity. Amplification of the variable regions V04 and V05 distinguished between American and the Spanish isolates. Biological characterization was performed against two laboratory colonies of S. exigua, one from Mexico, and another from Switzerland. Insects from the Mexican colony were less susceptible to infection than insects from Se-Swiss colony. In the Se-Mex colony, SeSP2 was the most pathogenic isolate followed by SeSIN6, although their virulence was similar to most of the isolates tested. In Se-Swiss colony, similar LD 50 values were observed for the five isolates, although the virulence was higher for the SeSLP6 isolate, which also had the highest OB (occlusion body) yield. We conclude that the Mexican isolates SeSIN6 and SeSLP6 possess insecticidal traits of value for the development of biopesticides for the control of populations of S. exigua. © 2017 The Authors.


Barajas-Ledesma E.,Universidad Michoacana de San Nicolás de Hidalgo | Garcia-Benjume M.L.,Universidad Michoacana de San Nicolás de Hidalgo | Espitia-Cabrera I.,Universidad Michoacana de San Nicolás de Hidalgo | Bravo-Patino A.,Centro Multidisciplinario Of Estudios En Biotecnologia | And 3 more authors.
Materials Science and Engineering B: Solid-State Materials for Advanced Technology | Year: 2010

Al2O3-doped TiO2 thin films were prepared by combining electrophoretic deposition (EPD) with sputtering. A Corning* glass was used as a substrate, in which a titanium film was deposited by sputtering. Then, a precursor sol was prepared with Ti(n-OBu)4 and Al(s-OBu)3 and used as the medium for EPD. Next, the thin films were sintered and, finally, characterised by scanning electron microscopy (SEM), high resolution transmission electron microscopy (HRTEM) and X-ray diffraction (XRD). Several cultures of Escherichia coli, strain XL1-Blue, were prepared. Nine experiments were carried out. In three of them, an inoculum (a low amount of a product that contains bacteria) was prepared without a film; in the other six Al2O3-doped TiO2 film-coated glass substrates were irradiated with visible light before they were introduced in the inoculum. The SEM and EDS results showed that TiO2-Al 2O3 films were obtained, covering all the glass substrate and with uniform size of particles forming them, and that the aluminium was distributed uniformly on the film. XRD results showed that rutile phase was obtained. By TEM, the structure of TiO2 was demonstrated. Al 2O3-doped TiO2 thin films were successful at eliminating E. coli. © 2010 Elsevier B.V. All rights reserved.


Guillen-Nepita A.L.,Centro Multidisciplinario Of Estudios En Biotecnologia | Vazquez-Marrufo G.,Centro Multidisciplinario Of Estudios En Biotecnologia | Blanco-Guillot F.T.,Laboratorio Estatal Of Salud Publica Of Michoacan | Figueroa-Aguilar G.A.,Laboratorio Estatal Of Salud Publica Of Michoacan | Vazquez-Garciduenas M.S.,Universidad Michoacana de San Nicolás de Hidalgo
New Microbiologica | Year: 2013

Random amplified polymorphism DNA (RAPD) is an easy, inexpensive technique for the characterization of pathogens in low-income countries. In this study we used RAPD to assess the genetic diversity of a small collection of isolates of mycobacteria from the Mexican state of Michoacán. In contrast with the low annual tuberculosis incidence in Michoacán relative to the national average, we found a high molecular diversity value suggesting high population diversity of M. tuberculosis in the studied region. Our findings justify further typing efforts with other molecular tools such as MIRU-VNTR and spoligotyping.


Vazquez-Marrufo G.,Centro Multidisciplinario Of Estudios En Biotecnologia | Vazquez-Garciduenas M.A.S.,Centro Multidisciplinario Of Estudios En Biotecnologia | Mukhtar I.,Centro Multidisciplinario Of Estudios En Biotecnologia
Bangladesh Journal of Botany | Year: 2014

During August and November, 2013, leaves of Euphorbia hirta L. were found with a rust disease in Taxco and Morelia cities in Guerrero and Michoacán states, respectively. The pustules of rust were orange - yellow to dark brown in color and were observed on lower and upper side of leaves. Necrotic red-purple or dark brown spots were also observed upper surface of leaves with pustules on the lower surface. On the basis of morphological characteristics, the rust species was identified as Uromyces euphorbiae. This is the new record of U. euphorbiae on E. hirta in Guerrero and Michoacán states in Mexico.

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