Fernandez-Ocana A.,University of Jaen |
Garcia-Lopez M.C.,University of Jaen |
Jimenez-Ruiz J.,University of Jaen |
Saniger L.,University of Jaen |
And 9 more authors.
Tree Genetics and Genomes
The juvenile-to-adult transition is a complex and poorly understood process in plant development required to reach reproductive competence. For woody plants, knowledge of this transition is even scantier and no genes have been definitively identified as involved in this transition. To search for genes involved in the juvenile-to-adult transition in olive, we constructed juvenile and adult subtractive cDNA gene libraries and identified genes that were differentially expressed in the juvenile and adult phases. In the analysis of theses libraries, we found 13 differentially expressed genes. One of these genes designated as juvenile to adult transition (JAT) was of special interest because it was highly expressed at the mRNA level in the early developmental phases but repressed in the adult phase. The analysis of mutant trees altered in the juvenile-to-adult transition, as well as a segregating progeny of 31 trees from a "Picual" x "Jabaluna" cross, support the contention that its activity might be required for a non-delayed transition. The study of an Arabidopsis thaliana JAT mutant strain confirmed this hypothesis as it showed a delayed flowering phenotype. JAT is expressed in different parts of the plant, showing an unexpectedly high level of mRNA in the roots. However, the JAT expression level is not determined by the distance to the roots, but rather depends on the developmental stage of the branch meristems. JAT is a widely represented gene in plants that appears to be involved in the control of the juvenile-to-adult transition in olive. © 2010 Springer-Verlag. Source
Lopez-Gomez M.,IRTA - Institute of Agricultural-Alimentary Research and Technology |
Talavera M.,Centro Camino Of Purchil |
Verdejo-Lucas S.,IRTA - Institute of Agricultural-Alimentary Research and Technology |
Verdejo-Lucas S.,Centro La Mojonera
The suitability of watermelon cultivars and cucurbit rootstocks as hosts to Meloidogyne incognita and M. javanica was determined in pot and field experiments. Meloidogyne incognita showed higher reproduction than did M. javanica on watermelon and cucurbit rootstocks. The watermelon cultivars did not differ in host status when challenged with these two species and supported lower nematode reproduction than the cucurbit rootstocks. Rootstocks Lagenaria siceraria cv. Pelops and Cucurbita pepo AK15 supported lower reproduction than did the squash hybrid rootstocks (C. maxima × C. moschata). Egg production increased (P < 0·05) with a rising initial inoculum level (Pi) in the non-grafted Sugar Baby but the reproduction factor Rf (eggs per plant/Pi) was similar at two Pi levels. The total egg production in the plants grafted onto squash hybrids RS841 and Titan was greater (P < 0·05) at the higher Pi, but the Rf values were lower. The development of field-grown non-grafted watermelon plants was significantly stunted in plots where nematodes were detected at planting. However, no differences were observed in plots with grafted plants. In plots with nematodes, non-grafted and Titan-grafted plants had similar yields that were higher than that of RS841-grafted plants. In the commercial plastic houses with grafted watermelon, the average Rf value was 42-fold, confirming the high susceptibility of squash hybrids as rootstocks for grafted watermelon. The Titan-Sugar Baby combination was tolerant to M. javanica. © 2016 British Society for Plant Pathology. Source
Obrero A.,IFAPA Centro del Llano |
Die J.V.,CSIC - Institute for Sustainable Agriculture |
Roman B.,IFAPA Centro del Llano |
Gomez P.,Centro La Mojonera |
And 2 more authors.
Journal of Agricultural and Food Chemistry
The zucchini (Cucurbita pepo) is an important food crop, the transcriptomics of which are a fundamental tool to accelerate the development of new varieties by breeders. However, the suitability of reference genes for data normalization in zucchini has not yet been studied. The aim of this study was to assess the suitability of 13 genes for their potential use as reference genes in quantitative real-time PCR. Assays were performed on 34 cDNA samples representing plants under different stresses and at different developmental stages. The application of geNorm and NormFinder software revealed that the use of a combination of UFP, EF-1A, RPL36aA, PP2A, and CAC genes for the different experimental sets was the best strategy for reliable normalization. In contrast, 18S rRNA and TUA were less stable and unsuitable for use as internal controls. These results provide the possibility to allow more accurate use of qPCR in this horticultural crop. © 2011 American Chemical Society. Source
Obrero A.,Centro Alameda del Obispo |
Gonzalez-Verdejo C.I.,Centro Alameda del Obispo |
Roman B.,Centro Alameda del Obispo |
Gomez P.,Centro La Mojonera |
And 2 more authors.
An essential step in the carotenoid biosynthesis pathway is the formation of phytoene by phytoene synthase (PSY). In this study, three new genes coding PSYs (CpPSYA, CpPSYB, and CpPSYC) were cloned from Cucurbita pepo and their expression patterns analysed in three cultivars of summer squash which had a different carotenoid content. The gene sequences had a high similarity with those from other plant species, and their predicted proteins were significantly different from each other. A phylogenetic analysis indicates that CpPSYA and CpPSYB shared a high homology and were also homologous with PSYs from others cucurbits, whereas CpPSYC was more closely related to orthologues from strawberry and carrot. An expression analysis revealed that CpPSYA had a higher expression in flowers compared to leaves and showed a differential expression during fruit development. The amount of CpPSYA transcript was higher in fruits with a higher carotenoid content than in those with a lower carotenoid content. However, CpPSYB and CpPSYC showed a relatively high expression in leaves, and their expression in fruits varied among the different cultivars and fruit tissues. These results suggest that the CpPSY genes were under different regulatory mechanisms and they may have different roles in C. pepo. © 2015, Springer Science+Business Media Dordrecht. Source
Villatoro-Pulido M.,IFAPA Centro del Llano |
Font R.,Centro La Mojonera |
Dominguez I.,Centro La Mojonera |
Del Rio-Celestino M.,Centro La Mojonera |
And 6 more authors.
The increase in life expectancy is a challenge in disease burden, especially, in chronic diseases like cancer. Several studies have stated the protection of some elements of Mediterranean diet (MD) to human health. The consequences of this protection on the chronic use of this kind of food are expressed as high standard of living quality. The present study develops different genetics, cytological and lifespan approaches to assess the protective role of components of the MD such as rocket, tomato, grapes and walnut oil. Source