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Rauscher G.,Cornell University | Rauscher G.,U.S. Department of Agriculture | Simko I.,Cornell University | Simko I.,U.S. Department of Agriculture | And 6 more authors.
Theoretical and Applied Genetics | Year: 2010

Genetic resistance is a valuable tool in the fight against late blight of potatoes but little is known about the stability and specificity of quantitative resistance including the effect of defeated major resistance genes. In the present study we investigated the effect of different isolates of Phytophthora infestans on the mode of action of RPi-ber, an R-gene originating from Solanum berthaultii. The experiments were conducted on progenies derived from two reciprocal inter-specific backcrosses of Solanum tuberosum and S. berthaultii. The plant-pathogen interaction was tested in diverse environments including field, greenhouse and growth chamber conditions. The RPi-ber gene provided complete resistance against a US8 isolate of P. infestans in all trials. When isolates compatible with RPi-ber were used for inoculation, a smaller, but significant resistance effect was consistently detected in the same map position as the R-gene. This indicates that this R-gene provides a residual resistance effect, and/or that additional resistance loci are located in this genomic region of chromosome X. Additional quantitative resistance loci (QRL) were identified in the analyzed progenies. While some of the QRL (such as those near TG130 on chromosome III) were effective against several isolates of the pathogen, others were isolate specific. With a single exception, the S. berthaultii alleles were associated with a decrease in disease severity. Resistance loci reported in the present study co-locate with previously reported R-genes and QRL to P. infestans and other pathogens. © 2010 US Government.

Rodriguez Pardina P.,Instituto Nacional de Tecnologia Agropecuaria | Luque A.,Instituto Nacional de Tecnologia Agropecuaria | Nome C.,Instituto Nacional de Tecnologia Agropecuaria | Lopez Colomba E.,Instituto Nacional de Tecnologia Agropecuaria | And 2 more authors.
Australasian Plant Disease Notes | Year: 2012

We report the complete nucleotide sequence of a begomovirus infecting sweet potato in the Northeastern region of Argentina. Sequence comparisons indicated that the isolate under study has the highest nucleotide sequence identity (93.6 %) with Sweet potato leaf curl virus Puerto Rico. According to the current taxonomic criteria for begomovirus classification the Argentinean isolate would correspond to a new strain of Sweet potato leaf curl virus. © 2012 Australasian Plant Pathology Society Inc.

Agency: Cordis | Branch: FP7 | Program: CP-SICA | Phase: KBBE-2008-1-4-08 | Award Amount: 3.85M | Year: 2009

VALORAM aims at exploring and valorizing Andean soil microbial diversity for the development of alternative, efficient technologies and crop management practices to improve the sustainability and productivity of Andean cropping systems benefiting rural farming households. The project will focus on potato because of its global importance for small-scale farmers in the central Andean highlands. The participants will use metagenomic, genomic, proteomic and metabolomic analysis to identify novel traits of microorganisms and characterize beneficial soil microbial communities, to achieve the objective. The project specific aims are to (1) explore the agro-ecosystem functions of soil microbes in potato-based cropping systems and preserve the components of this microflora in international culture collections, (2) elucidate the role of rhizosphere microorganisms and communities in promoting plant growth, suppressing soil borne disease and priming plant biotic defenses, developing eco-efficient technologies/products for sustainable crop production systems, (3) develop applied technologies and knowledge-based systems to improve the sustainability and resilience of potato based cropping systems for the benefit of the indigenous farmers and (4) promote the exchange of scientific knowledge and technologies among partners and the LA scientific community to impulse research in this area and support the continuous development of crop production technologies. The strategy for VALORAM implementation is to engage LA and EU partners in developing and further stengthening collaborative research activities in order to sustainably improve potato-based systems. This is supported by a multidisciplinary team of experts with highly complementary skills and based on a robust management structure with an efficient workshop and communication programme. The results will directly benefit the local partners and may also contribute to increase the productivity of organic potato production in the EU.

Agency: Cordis | Branch: FP7 | Program: CP-FP | Phase: KBBE-2009-1-4-01 | Award Amount: 3.99M | Year: 2010

Detection methods are the first tools used by national plant protection organisations (NPPO) and inspection services in order to find incursions of quarantine plant pathogens or pests (Q-pests) across a border, a crucial step to implement Council Directive 2000/29/EC. This is often done visually in the first instance, with support from a laboratory for confirmatory testing and subsequent monitoring. Reliance on laboratory testing causes significant delays when action is only taken on the return of results from the laboratory to which the samples were sent. Thus, there is a real need for rapid, simple and robust detection methods that can be deployed by NPPOs in the field with inspection services to enable early detection of Q-pests. The Q-detect consortium aims to develop detection methods based on biochemical (detecting volatile organic compounds [VOC] and nucleic acid), acoustic (including resonance), remote imaging (incorporating spectral and automated data analysis) and pest trapping (insect pests and pathogen vectors) techniques. The careful selection of traded products (primarily potato and forestry/trees) ensures the methods will be developed on high priority targets for the EU such as the pine wood nematode (Bursaphelenchus xylophilus), potato brown rot (Ralstonia solanacearum) and potato ring rot (Clavibacter michiganensis ssp. Sepedonicus), Asian longhorn beetle (Anoplophora glabripennis) and a range of whitefly transmitted viruses. The diversity of targets enables the Q-detect consortium to work on suites of complementary techniques; this is of particular importance since the diverse range of targets listed in Directive 2000/29/EC means no single detection method will be suitable for all Q-pests. Critically, NPPOs and third country institutes are partners, which will enable testing, and validation of methods at real outbreak sites where these are absent in the EU. SME partners ensure access to technology and routes for exploitation after the project ends.

Agency: Cordis | Branch: H2020 | Program: RIA | Phase: SFS-07b-2015 | Award Amount: 6.89M | Year: 2016

G2P-SOL is a research alliance bringing together the major European and International repositories of germplasm with public and private institutions active in genomics, phenotyping and breeding in the four major Solanaceous crops: potato, tomato, pepper and eggplant. These four crops constitute 66% of the value of European horticultural production, and over 65,000 accessions are available within the consortium. By harnessing the available global biodiversity, novel genotyping and phenotyping concepts and data analysis tools, the G2P-SOL project will link the genetic code underlying Solanaceae biodiversity with the traits that improve productivity, adaptation and human health. By making this information accessible to end-users, the awareness of the available diversity will be increased and use of this genetic diversity in breeding programs will be stimulated, resulting in diversified production chains. The phenotypes and traits of material held in European and major international collections will be described using common ontology terms developed in this project and this information will be housed in an open source software platform, allowing easy interfacing with existing platforms for germplasm cataloguing. G2P-SOL will develop shared values in science and education in the following areas: 1) Defining and maintaining genetic pools for crop improvement. 2) Phenomic and genomic data: generation, analysis, storage, and linkage with gene banks. 3) Pre-breeding and germplasm enhancement. 4) Training, workshops and public outreach. G2P-SOL will redefine how to manage and organize genetic resources and linked genomic and phenomic information in a manner that will make them accessible to naturalists, geneticists and breeders for conservation, scientific research, and breeding in the post genomic era, in compliance with the objectives of the International Treaty on Plant Genetic Resources and the Nagoya Protocol.

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