Time filter

Source Type

Corso M.,University of Padua | Corso M.,Centro Interdipartimentale per la Ricerca in Viticoltura Ed Enologia CIRVE | Vannozzi A.,University of Padua | Vannozzi A.,Centro Interdipartimentale per la Ricerca in Viticoltura Ed Enologia CIRVE | And 19 more authors.
Journal of Experimental Botany | Year: 2015

In light of ongoing climate changes in wine-growing regions, the selection of drought-tolerant rootstocks is becoming a crucial factor for developing a sustainable viticulture. In this study, M4, a new rootstock genotype that shows tolerance to drought, was compared from a genomic and transcriptomic point of view with the less drought-tolerant genotype 101.14. The root and leaf transcriptome of both 101.14 and the M4 rootstock genotype was analysed, following exposure to progressive drought conditions. Multifactorial analyses indicated that stress treatment represents the main factor driving differential gene expression in roots, whereas in leaves the genotype is the prominent factor. Upon stress, M4 roots and leaves showed a higher induction of resveratrol and flavonoid biosynthetic genes, respectively. The higher expression of VvSTS genes in M4, confirmed by the accumulation of higher levels of resveratrol in M4 roots compared with 101.14, was coupled to an up-regulation of several VvWRKY transcription factors. Interestingly, VvSTS promoter analyses performed on both the resequenced genomes highlighted a significantly higher number of W-BOX elements in the tolerant genotype. It is proposed that the elevated synthesis of resveratrol in M4 roots upon water stress could enhance the plant's ability to cope with the oxidative stress usually associated with water deficit. © 2015 The Author.


Vannozzi A.,University of Padua | Vannozzi A.,Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia CIRVE | Corso M.,University of Padua | Corso M.,Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia CIRVE | And 6 more authors.
Acta Horticulturae | Year: 2016

Iron chlorosis is an important nutritional disease that affects orchards and vineyards reducing quality and yield production. In soil, high calcareous conditions affect pH, raising it to sub-alkaline and alkaline levels and maintaining iron in an insoluble form as Fe3+. Chlorotic plants show abnormal photosynthesis and yellowing shoots. Nowadays the most used protection against iron chlorosis is grafting the cultivars of economic interest on tolerant rootstocks. Genetic improvement of fruit trees plays an important role on the selection of iron chlorosis resistant rootstocks using breeding techniques. In grapevine, this selection is based on breeding cultivars belonging to the V. vinifera species with non-vinifera ones (i.e., wild American species). In grapevine, iron uptake and homeostasis are controlled by a mechanism known as "Strategy I", characteristic of non-graminaceous plants and based on a system of iron reduction, soil acidification and transporters-mediated uptake. In this study, main Strategy I-genes involved in iron uptake were identified and analysed in two grapevine rootstocks characterized by different levels of tolerance and susceptibility to iron chlorosis. Expression analyses on selected genes under different iron-availability conditions revealed important differences which could explain the different degree of tolerance observed in the two genotypes under study. © 2016, International Society for Horticultural Science. All rights reserved.


Corso M.,University of Padua | Ziliotto F.,University of Padua | Rizzini F.M.,University of Padua | Teo G.,Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia CIRVE | And 3 more authors.
Plant Science | Year: 2013

At ripening, Vitis vinifera cv Raboso Piave grapes have high acidity, which results in an astringent wine that is not easy to drink. To overcome this limitation, several researches have attempted to alter the polyphenols profile mainly by applying different harvest techniques. The aim of this work was to investigate sensorial, biochemical, and molecular changes in Raboso Piave grape berries subjected to delayed harvests as Late Harvest (LH) and "Double Maturation Raisonnée" (DMR) techniques. At the molecular level, a microarray study was conducted comparing Traditional Harvest berries (TH) to LH and DMR ones. Gene ontology enrichment analysis pointed out that LH and DMR techniques affected metabolism of acids, sugars and polyphenols. A Principal Component Analysis, performed on transcriptomic data, pointed out that malate catabolism as well as some branches of flavonoids biosynthesis are significantly affected by DMR. In DMR grape berries, the flavonol and catechin accumulations were induced and depressed, respectively. In parallel, the transcription of flavonol synthase and leucoanthocyanidin-reductase 2, the main genes responsible for flavonol and catechin biosynthesis, were similarly induced and down-regulated. These changes resulted in a brighter colored wine with lower astringency. © 2013 Elsevier Ireland Ltd.

Loading Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia CIRVE collaborators
Loading Centro Interdipartimentale per la Ricerca in Viticoltura ed Enologia CIRVE collaborators