Herance R.,Institute dAlta Tecnologia |
Herance R.,Centro Imagen Molecular |
Herance R.,Research Center Biomedica en Red en Bioingenieria |
Herance R.,University Pompeu Fabra |
And 40 more authors.
Molecular Imaging | Year: 2011
In this study, we assessed the feasibility of using positron emission tomography (PET) and the tracer [ 11C]OMAR ([ 11C]JHU75528), an analogue of rimonabant, to study the brain cannabinoid type 1 (CB1) receptor system. Wild-type (WT) and CB1 knockout (KO) animals were imaged at baseline and after pretreatment with blocking doses of rimonabant. Brain uptake in WT animals was higher (50%) than in KO animals in baseline conditions. After pretreatment with rimonabant, WT uptake lowered to the level of KO animals. The results of this study support the feasibility of using PET with the radiotracer [ 11C]JHU75528 to image the brain CB1 receptor system in mice. In addition, this methodology can be used to assess the effect of new drugs in preclinical studies using genetically manipulated animals. © 2011 Decker Publishing.
Torrent E..,Centro Imagen Molecular |
Torrent E..,Autonomous University of Barcelona |
Farre M.,Centro Imagen Molecular |
Farre M.,Autonomous University of Barcelona |
And 11 more authors.
Molecular Imaging | Year: 2013
The goal of this study was to compare different quantification approaches and reconstruction methods to estimate the binding potential in [ 11C]raclopride studies in rats. The final aim was to determine if the results obtained with short-acquisition scanning were comparable to the results obtained with long-acquistion (conventional) scanning. We analyzed two rat data sets: A baseline versus a pretreatment study (with cold raclopride) and a young versus an old animal group comparison. The study results support the contention that optimization of [11C]raclopride positron emission tomographic studies in rats by shortening the acquisition time is feasible. In addition, filtered backprojection is recommended as a reconstruction algorithm, although iterative methods may be more sensitive to detect within-group differences. © 2013 Decker Publishing.