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Vila Real de Santo António, Portugal

Reis R.,Centro Hospitalar Of Tras Os Montes E Alto Douro | Teixeira F.,Centro Hospitalar iversitario Of Coimbra | Martins V.,Centro Hospitalar iversitario Of Coimbra | Sousa L.,Centro Hospitalar iversitario Of Coimbra | And 3 more authors.
Revista Portuguesa de Pneumologia | Year: 2015

This article has been withdrawn at the request of the author(s) and/or editor. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. © 2014 Sociedade Portuguesa de Pneumologia.

Soares Pires F.,Centro Hospitalar Of Sao Joao | Drummond M.,Centro Hospitalar Of Sao Joao | Drummond M.,University of Porto | Marinho A.,Centro Hospitalar Of Sao Joao | And 10 more authors.
Sleep and Breathing | Year: 2013

Purpose: Auto-titrating continuous positive airway pressure (APAP) is an effective treatment for obstructive sleep apnea/ hypopnea syndrome (OSAHS). We investigated whether a single group education session on APAP therapy is effective in promoting adherence among patients with OSAHS. Method:s This prospective, randomized, controlled, parallel group study included patients newly diagnosed with OSAHS who met criteria for APAP therapy. Patients were randomized into a study group and a control group. All patients in the study group were assigned to a single group education session, 1 month after beginning APAP therapy. Results: We evaluated 146 patients. The median percentage of APAP usage days was 88.3 %, with a median duration per day of use of 6.02 h; 59%were classified as adherent. Overall, no significant difference in adherence was seen between the study and the control groups. Analyzing patient subgroups, the group session significantly improved APAP adherence among males and patients who were younger (<65 years old), obese (BMI ≥35 kg/m2), non-sleepy (Epworth sleepiness scale ≤11), smokers or past smokers, had hypertension or nocturia and those with non-severe OSAHS. Conclusion: To maximize the impact of group education sessions and, by that, saving resources, it may be important to select patients likely to benefit from these sessions. © Springer-Verlag Berlin Heidelberg 2013.

Alves M.J.,Catholic University of Portugal | Alves M.J.,Centro Hospitalar Of Tras Os Montes E Alto Douro | Alves M.J.,Polytechnic Institute of Braganca | Ferreira I.C.F.R.,Polytechnic Institute of Braganca | And 2 more authors.
Journal of Applied Microbiology | Year: 2012

Aims: This work aimed to screen the antimicrobial activity of aqueous methanolic extracts of 13 mushroom species, collected in Bragança, against several clinical isolates obtained in Hospital Center of Trás-os-Montes and Alto Douro, Portugal. Methods and Results: Microdilution method was used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). MIC results showed that Russula delica and Fistulina hepatica extracts inhibited the growth of gram-negative (Escherichia coli, Morganella morganni and Pasteurella multocida) and gram-positive (Staphylococcus aureus, MRSA, Enterococcus faecalis, Listeria monocytogenes, Streptococcus agalactiae and Streptococcus pyogenes) bacteria. A bactericide effect of both extracts was observed in Past. multocida, Strep. agalactiae and Strep. pyogenes with MBC of 20, 10 and 5 mg ml-1, respectively. Lepista nuda extract exhibited a bactericide effect upon Past. multocida at 5 mg ml-1 and inhibited Proteus mirabilis at 20 mg ml-1. Ramaria botrytis extract showed activity against Enterococcus faecalis and L. monocytogenes, being bactericide for Past. multocida, Strep. agalactiae (MBCs 20 mg ml-1) and Strep. pyogenes (MBC 10 mg ml-1). Leucopaxillus giganteus extract inhibited the growth of E. coli and Pr. mirabilis, being bactericide for Past. multocida, Strep. pyogenes and Strep. agalactiae. Conclusions: Fistulina hepatica, R. botrytis and R. delica are the most promising species as antimicrobial agents. Significance and Impact of the Study: Mushroom extracts could be an alternative as antimicrobials against pathogenic micro-organisms resistant to conventional treatments. © 2012 The Society for Applied Microbiology.

Alves M.J.,Catholic University of Portugal | Alves M.J.,Centro Hospitalar Of Tras Os Montes E Alto Douro | Alves M.J.,Polytechnic Institute of Braganca | Ferreira I.C.F.R.,Polytechnic Institute of Braganca | And 4 more authors.
Journal of Applied Microbiology | Year: 2013

Aim and Methods: Although the antimicrobial activity of extracts from several mushroom species has been reported, studies with the individual compounds present in that extracts are scarce. Herein, the antimicrobial activity of different phenolic compounds identified and quantified in mushroom species from all over the world was evaluated. Furthermore, a structure-activity relationship (SAR) analysis and molecular docking studies were performed, in order to provide insights into the mechanism of action of potential antimicrobial drugs for resistant micro-organisms. Results: 2,4-Dihydroxybenzoic and protocatechuic acids were the phenolic compounds with higher activity against the majority of Gram-negative and Gram-positive bacteria. Furthermore, phenolic compounds inhibited more MRSA than methicillin-susceptible Staphylococcus aureus. MRSA was inhibited by 2,4-dihydroxybenzoic, vanillic, syringic (MICs = 0·5 mg ml-1) and p-coumaric (MIC = 1 mg ml-1) acids, while these compounds at the same concentrations had no inhibitory effects against methicillin-susceptible Staph. aureus. Conclusions: The presence of carboxylic acid (COOH), two hydroxyl (OH) groups in para and ortho positions of the benzene ring and also a methoxyl (OCH3) group in the meta position seems to be important for anti-MRSA activity. Significance and Impact of the Study: Phenolic compounds could be used as antimicrobial agents, namely against some micro-organisms resistant to commercial antibiotics. © 2013 The Society for Applied Microbiology.

Amaral C.,University of Beira Interior | Amaral C.,University of Porto | Gallardo E.,University of Beira Interior | Pinto Leite R.,Centro Hospitalar Of Tras Os Montes E Alto Douro | And 2 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2010

Cholesterol and its precursors, namely 7-dehydrocholesterol, desmosterol and lathosterol are important biochemical markers of cholesterol biosynthesis, and their quantification in body fluids is useful for the diagnosis of cholesterol biosynthesis pathway disorders. A rapid and sensitive gas chromatographic-mass spectrometric method was developed and validated for quantitative analysis of five sterols (cholesterol, 7-dehydrocholesterol, desmosterol, lathosterol and sitosterol) in amniotic fluid. The method was linear for all compounds (r2>0.99), and intra and inter-assay coefficients of variation were typically below 5%, and inaccuracy was within a ±12% interval. The method was applied to 330 amniotic fluid samples, grouped by gestational age between 13 and 22 weeks of pregnancy, in order to establish reference intervals for sterols in this specimen. The obtained concentrations (μmol/L) for each sterol was as follows: 22.1758±4.2716 at 13 weeks and 78.5082±12.9041 at 22 weeks for cholesterol; 0.0039±0.0007 at 13 weeks and 0.1150±0.0212 at 22 weeks for 7-dehydrocholesterol; 0.1562±0.0406 at 13 weeks and 0.7691±0.0821 at 22 weeks for desmosterol; 0.0272±0.0035 at 13 weeks and 0.8551±0.1791 at 22 weeks for lathosterol; and 0.0404±0.0039 at 13 weeks and 0.2326±0.0386 at 22 weeks for sitosterol. The method was also applied to one pathological sample that showed decreased levels of cholesterol, and higher concentration of 7-dehydrocholesterol, which is consistent with a 7-dehydrocholesterol-reductase deficiency. Our results showed that as long as pregnancy goes on, the concentrations of cholesterol and precursors increase in amniotic fluid, which is related to the increased need for cholesterol by the fetus. The reference range of each sterol in amniotic fluid was calculated at different gestational ages and will be useful for the interpretation and validation of biochemical prenatal diagnosis of inborn errors of sterol biosynthesis. © 2010 Elsevier B.V.

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