Carvalhais V.,University of Aveiro |
Carvalhais V.,University of Minho |
Cerveira F.,Centro Hospitalar Baixo Vouga |
Vilanova M.,IBMC Institute Biologia Molecular e Celular |
And 3 more authors.
Molecular Immunology | Year: 2015
Virulence of Staphylococcus epidermidis is mainly attributed to surface colonization and biofilm formation in indwelling medical devices. Physiological heterogeneity of biofilms may influence host immune response and sensitivity to antibiotics. Dormant cells, among others, contribute to biofilm heterogeneity. The aim of this study was to identify immunogenic proteins of S. epidermidis biofilms associated with dormancy mechanism, by using two-dimensional electrophoresis (2-DE) immunoblotting and mass spectrometry (MS). A total of 19 bacterial proteins, recognized by human serum samples, were identified. These proteins were mainly involved in small molecule metabolic biological processes. Catalytic activity and ion binding were the most representative molecular functions. CodY and GpmA proteins were more reactive to sera when biofilm dormancy was induced, while FtnA and ClpP were more reactive when dormancy was prevented. This is the first work that identifies differences in immunoreactive proteins within bacterial biofilms with induced or prevented dormancy. Considering the importance of dormancy within biofilms, further evaluation of these proteins can provide insights into the mechanisms related to dormancy and help to improve current understanding on how dormancy affects the host immune response. © 2015 Elsevier Ltd.
PubMed | University of Minho, Abel Salazar Biomedical Sciences Institute, Centro Hospitalar Baixo Vouga and University of Aveiro
Type: Journal Article | Journal: Molecular immunology | Year: 2015
Virulence of Staphylococcus epidermidis is mainly attributed to surface colonization and biofilm formation in indwelling medical devices. Physiological heterogeneity of biofilms may influence host immune response and sensitivity to antibiotics. Dormant cells, among others, contribute to biofilm heterogeneity. The aim of this study was to identify immunogenic proteins of S. epidermidis biofilms associated with dormancy mechanism, by using two-dimensional electrophoresis (2-DE) immunoblotting and mass spectrometry (MS). A total of 19 bacterial proteins, recognized by human serum samples, were identified. These proteins were mainly involved in small molecule metabolic biological processes. Catalytic activity and ion binding were the most representative molecular functions. CodY and GpmA proteins were more reactive to sera when biofilm dormancy was induced, while FtnA and ClpP were more reactive when dormancy was prevented. This is the first work that identifies differences in immunoreactive proteins within bacterial biofilms with induced or prevented dormancy. Considering the importance of dormancy within biofilms, further evaluation of these proteins can provide insights into the mechanisms related to dormancy and help to improve current understanding on how dormancy affects the host immune response.
Marto G.,Centro Hospitalar Baixo Vouga |
Aguiar R.,Centro Hospitalar Baixo Vouga |
Barcelos A.,Centro Hospitalar Baixo Vouga
Acta Reumatologica Portuguesa | Year: 2014
Systemic sclerosis (SSc) is a rare disease with an increased incidence of cancer, but the ocorrence of Non- -Hodgkin lymphoma (NHL) is a very uncommon event. We report a case of a 76-year-old female addmi - ted to the hematology clinic with long-term adenopa - thies and ocasional gastro-intestinal symptomatology. Progressive symmetrical swelling of hands was also noticed. Colonoscopy revealed multiple polyps and histopathology was consistent with Mantle-Cell Lym - phoma (MCL)-NHL. R-CHOP (rituximab, cyclophosphamide, doxorubicine, vincristine and prednisone) regimen was promptly iniated with complete response. Persistent swelling of both hands was observed, with thickening of the skin of both handswith proximal extension until the forearm. Biopsy confirmed the diagnosis of scleroderma.Symptomatic and rehabilitation treatment was initiated with mild improvement of symptoms.To our knowledge this is the first case of MCL associated with SSc.
Barcelos A.,Centro Hospitalar Baixo Vouga |
Fernandes B.,Instituto Portugues Of Oncologia
Acta Reumatologica Portuguesa | Year: 2012
Neonatal Lupus Erythematosus (NLE) is a rare disease associated with placental transport of maternal anti-Ro/ /La and/or anti-U1RNP antibodies into the fetal circula -tion and characterized by cardiac, cutaneous, hematologic and hepatic manifestations. The most serious com plication of NLE is complete heart block and cardiomyopathy. The maternal connective tissue disorder has been systemic lupus erythematosus (SLE) or Sjögren syndrome in most cases, however approximately 50% of mothers are asymptomatic at the time of diagnosis, testing positive only against Ro and/ or U1RNP auto-antibodies. We describe a case of neonatal lupus erythematosus and review the clinical and laboratory manifestations of this rare disease.
Verissimo L.M.P.,University of Coimbra |
Valada T.I.C.,University of Coimbra |
Sobral A.J.F.N.,University of Coimbra |
Azevedo E.E.F.G.,University of Coimbra |
And 2 more authors.
Journal of Chemical Thermodynamics | Year: 2014
The Taylor dispersion technique has been used for measuring mutual diffusion coefficients of sodium hyaluronate in aqueous solutions at T = 298.15 K, and concentrations ranging from (0.00 to 0.50) g · dm-3. The results are interpreted on the basis of Nernst, and Onsager and Fuoss theoretical equations. From the diffusion coefficient at infinitesimal concentration, the limiting ionic conductivity and the tracer diffusion coefficient of hyaluronate ion were estimated. These studies have been complemented by molecular mechanics calculations. © 2013 Elsevier Ltd. All rights reserved.
Lopes A.C.G.A.,Centro Hospitalar Baixo Vouga |
Nunes S.I.C.V.,Centro Hospitalar Baixo Vouga
Jornal Brasileiro de Psiquiatria | Year: 2015
The authors report the case of a 61 years-old woman, with history of depressive episodes and syncope, who developed hyperreligiosity, grandiose delusions and déjà vu. She was admitted to a psychiatry emergency and the electroencephalogram identified temporal lobe epilepsy. She was medicated with valproate sodium 750 mg/day with remission of psychotic symptoms and return to premorbid function. The purpose of this work is to highlight the importance of organic investigation in the assessment of neuropsychiatric symptoms. © 2015, Editora Cientifica Nacional Ltda. All rights reserved.
Dias E.,University of Aveiro |
Dias E.,Centro Hospitalar Baixo Vouga |
Gomes M.,University of Aveiro |
Domingues C.,University of Aveiro |
And 3 more authors.
Journal of Toxicology and Environmental Health - Part A: Current Issues | Year: 2013
Thiodicarb, a carbamate pesticide widely used on crops, may pose several environmental and health concerns. This study aimed to explore its toxicological profile on male rats using hematological, biochemical, histopathological, and flow cytometry markers. Exposed animals were dosed daily at 10, 20, or 40 mg/kg/body weight (group A, B, and C, respectively) during 30 d. No significant changes were observed in hematological parameters among all groups. After 10 d, a decrease of total cholesterol levels was noted in rats exposed to 40 mg/kg. Aspartate aminotransferase (AST) activity increased (group A at 20 d; groups A and B at 30 d) and alkaline phosphatase (ALP) (group B at 30 d) activity significantly reduced. At 30 d a decrease of some of the other evaluated parameters was observed with total cholesterol and urea levels in group A as well as total protein and creatinine levels in groups A and B. Histological results demonstrated multi-organ dose-related damage in thiodicarb-exposed animals, evidenced as hemorrhagic and diffuse vacuolation in hepatic tissue; renal histology showed disorganized glomeruli and tubular cell degeneration; spleen was ruptured with white pulp and clusters of iron deposits within red pulp; significant cellular loss was noted at the cortex of thymus; and degenerative changes were observed within testis. The histopathologic alterations were most prominent in the high-dose group. Concerning flow cytometry studies, an increase of lymphocyte number, especially T lymphocytes, was seen in blood samples from animals exposed to the highest dose. Taken together, these results indicate marked systemic organ toxicity in rats after subacute exposure to thiodicarb. © 2013 Taylor and Francis Group, LLC.
Gil P.,Centro Hospitalar Baixo Vouga
Journal of Glaucoma | Year: 2016
PURPOSE OF THE STUDY:: The purpose of the study was to describe and compare anterior and posterior topographic elevation maps in primary open angle glaucoma patients with functional damage staging and in healthy controls. METHODS:: A total of 217 subjects were consecutively recruited, including 111 primary open angle glaucoma patients (patients), and 106 healthy individuals (controls). All patients performed Pentacam HR corneal topography. Mean anterior keratometry and anterior and posterior topographic elevation maps were compared in the central 3, 5, and 7 mm. Humphrey automated perimetry results from the patient group were classified according to the Glaucoma Staging System. RESULTS:: Age (patients: 72.32±8.09; controls: 70.82±8.36; P=0.18) and central corneal pachymetry (patients: 541.13±36.98; controls: 548.67±34.56; P=0.12) were similar in both groups. Maximum elevation readings in the central 5 mm were significantly (P<0.05) higher in the anterior (patients: 8.21±8.63; controls: 5.79±3.62) and posterior (patients: 16.17±8.72; controls: 13.92±6.03) corneal topography of the glaucomatous patients, as well as in the anterior (patients: 17.32±20.78; controls: 9.61±5.64) and posterior (patients: 38.81±19.78; controls: 26.38±12.73) central 7 mm. There was a weak but significant correlation between the Glaucoma Staging System stage and both the anterior 5 mm (r=0.397) and 7 mm (r=0.304) maximum, as well as the posterior 5 mm (r=0.233) and 7 mm (r=0.241) maximum. CONCLUSIONS:: In patients with primary open angle glaucoma, there is a forward shifting of the posterior and anterior corneal surfaces. This appears to be correlated with more advanced stages of functional damage, pointing to a possible link between corneal structural changes and duration and intensity of elevated intraocular pressure. Further studies may ascertain the potential for this biological marker to be used in monitoring primary open angle glaucoma patients. Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved.
PubMed | Centro Hospitalar Baixo Vouga
Type: Journal Article | Journal: Reumatologia | Year: 2016
Eosinophilic fasciitis is a systemic inflammatory disease characterized by symmetrical swelling and skin induration of the distal portions of the arms and/or legs, evolving into a scleroderma-like appearance, accompanied by peripheral blood eosinophilia. It is a rare disease with a poorly understood etiology. Corticosteroid treatment remains the standard therapy, either taken alone or in association with an immunosuppressive drug. This paper presents a case of a male patient with palpebral edema and marked eosinophilia, diagnosed with intestinal parasitic infection in October 2006. He was treated with an antiparasitic drug, but both the swelling and the analytical changes remained. This was followed by a skin and muscle biopsy, which turned out to be compatible with eosinophilic fasciitis. There was progressive worsening of the clinical state, with stiffness of the abdominal wall and elevated inflammatory parameters, and the patient was referred to the Immunology Department, medicated with corticosteroids and methotrexate. Over the years there were therapeutic adjustments and other causes were excluded. Currently the patient continues to be monitored, and there is no evidence of active disease. The case described in this article is interesting because of the diagnosis of eosinophilic fasciitis probably associated/coexisting with a parasite infection. This case report differs from others in that there is an uncommon cause associated with the onset of the disease, instead of the common causes such as trauma, medication, non-parasitic infections or cancer.
PubMed | IBMC Institute Biologia Molecular e Celular, Centro Hospitalar Baixo Vouga and University of Aveiro
Type: Journal Article | Journal: Electrophoresis | Year: 2015
Saliva is essential to interact with microorganisms in the oral cavity. Therefore, the interest in saliva antimicrobial properties is on the rise. Here, we used an immunoproteomic approach, based on protein separation of Staphylococcus epidermidis biofilms by 2DE, followed by Western-blotting, to compare human serum and saliva reactivity profile. A total of 17 proteins were identified by MALDI-TOF/TOF. Serum and saliva presented a distinct pattern of immunoreactive proteins. Our results suggest that saliva seems to have higher propensity to react against S. epidermidis proteins with oxidoreductase activity and proteins involved with L-serine metabolic processes. We show that saliva was a powerful tool for the identification of potential S. epidermidis biofilms proteins.