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Fajã de Baixo, Portugal

Barcelos A.,Centro Hospitalar Baixo Vouga | Fernandes B.,Instituto Portugues Of Oncologia
Acta Reumatologica Portuguesa | Year: 2012

Neonatal Lupus Erythematosus (NLE) is a rare disease associated with placental transport of maternal anti-Ro/ /La and/or anti-U1RNP antibodies into the fetal circula -tion and characterized by cardiac, cutaneous, hematologic and hepatic manifestations. The most serious com plication of NLE is complete heart block and cardiomyopathy. The maternal connective tissue disorder has been systemic lupus erythematosus (SLE) or Sjögren syndrome in most cases, however approximately 50% of mothers are asymptomatic at the time of diagnosis, testing positive only against Ro and/ or U1RNP auto-antibodies. We describe a case of neonatal lupus erythematosus and review the clinical and laboratory manifestations of this rare disease.

Carvalhais V.,University of Aveiro | Carvalhais V.,University of Minho | Cerveira F.,Centro Hospitalar Baixo Vouga | Vilanova M.,IBMC Institute Biologia Molecular e Celular | And 3 more authors.
Molecular Immunology | Year: 2015

Virulence of Staphylococcus epidermidis is mainly attributed to surface colonization and biofilm formation in indwelling medical devices. Physiological heterogeneity of biofilms may influence host immune response and sensitivity to antibiotics. Dormant cells, among others, contribute to biofilm heterogeneity. The aim of this study was to identify immunogenic proteins of S. epidermidis biofilms associated with dormancy mechanism, by using two-dimensional electrophoresis (2-DE) immunoblotting and mass spectrometry (MS). A total of 19 bacterial proteins, recognized by human serum samples, were identified. These proteins were mainly involved in small molecule metabolic biological processes. Catalytic activity and ion binding were the most representative molecular functions. CodY and GpmA proteins were more reactive to sera when biofilm dormancy was induced, while FtnA and ClpP were more reactive when dormancy was prevented. This is the first work that identifies differences in immunoreactive proteins within bacterial biofilms with induced or prevented dormancy. Considering the importance of dormancy within biofilms, further evaluation of these proteins can provide insights into the mechanisms related to dormancy and help to improve current understanding on how dormancy affects the host immune response. © 2015 Elsevier Ltd.

Verissimo L.M.P.,University of Coimbra | Valada T.I.C.,University of Coimbra | Sobral A.J.F.N.,University of Coimbra | Azevedo E.E.F.G.,University of Coimbra | And 2 more authors.
Journal of Chemical Thermodynamics | Year: 2014

The Taylor dispersion technique has been used for measuring mutual diffusion coefficients of sodium hyaluronate in aqueous solutions at T = 298.15 K, and concentrations ranging from (0.00 to 0.50) g · dm-3. The results are interpreted on the basis of Nernst, and Onsager and Fuoss theoretical equations. From the diffusion coefficient at infinitesimal concentration, the limiting ionic conductivity and the tracer diffusion coefficient of hyaluronate ion were estimated. These studies have been complemented by molecular mechanics calculations. © 2013 Elsevier Ltd. All rights reserved.

Carvalhais V.,University of Aveiro | Carvalhais V.,University of Minho | Amado F.,University of Aveiro | Cerveira F.,Centro Hospitalar Baixo Vouga | And 5 more authors.
Electrophoresis | Year: 2015

Saliva is essential to interact with microorganisms in the oral cavity. Therefore, the interest in saliva antimicrobial properties is on the rise. Here, we used an immunoproteomic approach, based on protein separation of Staphylococcus epidermidis biofilms by 2DE, followed by Western-blotting, to compare human serum and saliva reactivity profile. A total of 17 proteins were identified by MALDI-TOF/TOF. Serum and saliva presented a distinct pattern of immunoreactive proteins. Our results suggest that saliva seems to have higher propensity to react against S. epidermidis proteins with oxidoreductase activity and proteins involved with L-serine metabolic processes. We show that saliva was a powerful tool for the identification of potential S. epidermidis biofilms proteins. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Pimentel-Santos F.M.,New University of Lisbon | Pimentel-Santos F.M.,University of Tras os Montes e Alto Douro | Pimentel-Santos F.M.,Centro Hospitalar Lisbon Ocidental CHLO | Matos M.,University of Tras os Montes e Alto Douro | And 15 more authors.
Arthritis Research and Therapy | Year: 2010

Introduction: A number of genetic-association studies have identified genes contributing to ankylosing spondylitis (AS) susceptibility but such approaches provide little information as to the gene activity changes occurring during the disease process. Transcriptional profiling generates a 'snapshot' of the sampled cells' activity and thus can provide insights into the molecular processes driving the disease process. We undertook a whole-genome microarray approach to identify candidate genes associated with AS and validated these gene-expression changes in a larger sample cohort.Methods: A total of 18 active AS patients, classified according to the New York criteria, and 18 gender- and age-matched controls were profiled using Illumina HT-12 whole-genome expression BeadChips which carry cDNAs for 48,000 genes and transcripts. Class comparison analysis identified a number of differentially expressed candidate genes. These candidate genes were then validated in a larger cohort using qPCR-based TaqMan low density arrays (TLDAs).Results: A total of 239 probes corresponding to 221 genes were identified as being significantly different between patients and controls with a P-value <0.0005 (80% confidence level of false discovery rate). Forty-seven genes were then selected for validation studies, using the TLDAs. Thirteen of these genes were validated in the second patient cohort with 12 downregulated 1.3- to 2-fold and only 1 upregulated (1.6-fold). Among a number of identified genes with well-documented inflammatory roles we also validated genes that might be of great interest to the understanding of AS progression such as SPOCK2 (osteonectin) and EP300, which modulate cartilage and bone metabolism.Conclusions: We have validated a gene expression signature for AS from whole blood and identified strong candidate genes that may play roles in both the inflammatory and joint destruction aspects of the disease. © 2011 Pimentel-Santos et al.; licensee BioMed Central Ltd.

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