Centro Especializado em Micologia Medica

Fortaleza, Brazil

Centro Especializado em Micologia Medica

Fortaleza, Brazil
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de Aguiar Cordeiro R.,Centro Especializado em Micologia Medica | Astete-Medrano D.J.,Programa de Pos Graduacao em Ciencias Medicas | de Farias Marques F.J.,Centro Especializado em Micologia Medica | Andrade H.T.L.,Centro Especializado em Micologia Medica | And 9 more authors.
Memorias do Instituto Oswaldo Cruz | Year: 2011

The aim of the present study was to evaluate the effect of cotrimoxazole on the in vitro susceptibility of Coccidioides posadasii strains to antifungals. A total of 18 strains of C. posadasii isolated in Brazil were evaluated in this study. The assays were performed in accordance with the Clinical and Laboratory Standards Institute guidelines and the combinations were tested using the checkerboard method. The minimum inhibitory concentrations were reduced by 11, 2.4, 4.3 and 3.5 times for amphotericin B, itraconazole, fluconazole and voriconazole, respectively. Moreover, it was seen that cotrimoxazole itself inhibited C. posadasii strains in vitro. The impairment of folic acid synthesis may be a potential antifungal target for C. posadasii.


Rocha M.F.G.,State University of Ceará | Rocha M.F.G.,Centro Especializado em Micologia Medica | de Aguiar F.L.N.,State University of Ceará | Brilhante R.S.N.,Centro Especializado em Micologia Medica | And 9 more authors.
Ciencia Rural | Year: 2011

The increase in the incidence of fungal infections and the frequent report of resistance and therapeutic failure has promoted the performance of phytochemical screening for compounds with antifungal properties. Based on this, the present study investigated the antifungal potential of extracts of Baccharis ligustrina, B. schultzii, Croton jacobinensis, Licania rigida, Moringa oleifera, Vernonia sp. and V. brasiliana and of essential oils of Lippia alba (Chemotypes 1, 2, 3 and 4) and Ocimum gratissimum. Initially, a qualitative evaluation of the antifungal activity of each vegetal sample against strains of Candida albicans and Microsporum canis, through the agar diffusion method, was performed. Extracts of M. oleifera (MLFC) and Vernonia sp. (TVS-H) presented activity against C. albicans and M. canis with inhibition halos =10mm. Then, minimum inhibitory concentrations (MICs) for MLF-C and TVS-H against 12 strains of C. albicans and M. canis were determined through the methodology established by the Clinical and Laboratory Standards Institute (CLSI), and acute toxicity tests against Artemia sp. were performed for both extracts. MICs (80%) for MLF-C and TVS-H varied from 0.156 to 2.5mg mL -1 against C. albicans and from 0.039 to 1.25mg mL -1 and 0.039 to 0.625mg mL -1against M. canis, respectively. MICs (100%) for MLF-C and TVS-H varied from 0.625 to 2.5mg mL -1 for C. albicans and from 0.039 to 2.5mg mL -1 and 0.078 to 1.25mg mL -1 against M. canis, respectively. Lethal doses (DL 50) of MLF-C and TVS-H were 201.09 and 204.17mg mL -1 respectively, being, therefore, demonstrated the low toxicity of these extracts. M. oleifera and Vernonia sp. extracts presented in vitro antifungal activity against C. albicans and M. canis, creating perspectives for the development of studies on the characterizations of their bioactive components.

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