Centro Agrario Of Albaladejito

Chillarón de Cuenca, Spain

Centro Agrario Of Albaladejito

Chillarón de Cuenca, Spain
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Usano-Alemany J.,Centro Agrario Of Albaladejito | Usano-Alemany J.,Complutense University of Madrid | Herraiz Penalver D.,Centro Agrario Of Albaladejito | Cuadrado Ortiz J.,Centro Agrario Of Albaladejito | And 3 more authors.
Botanica Complutensis | Year: 2011

In the present paper, we present a description of the ecological agriculture of different lavenders in Cuenca province (Spain). We focused on the important aromatic crops of Lavandula angustifolia (lavender) and on three varieties of Lavandula x intermedia (lavandin) called name abrial, rosso and super. A description of yield production and essential oil qualities obtained in our experimental fields are presented. Lavandin var. super showed the best essential oil quality with good yield production as well. The current market of Fragrant and Medicinal Plants claims raw materials with confirmed quality, with good practices of harvesting, transformation and manufacture by means of ecological production.


Usano-Alemany J.,University of Bonn | Pala-Paul J.,Complutense University of Madrid | Rodriguez M.S.-C.,Centro Agrario Of Albaladejito | Herraiz-Penalver D.,Centro Agrario Of Albaladejito
Natural Product Communications | Year: 2014

The amount and chemical composition of essential oils are crucial for the modulation of the flavor, scent and therapeutic properties of aromatic and medicinal plants. The aim of this study was to evaluate the effects of phenology and weather conditions on the essential oil yield obtained from the aerial parts of Salvia lavandulifolia Vahl. Besides, we tried to carry out an approach to the chemical composition at the time of full bloom. Essential oil production of several accessions was monitored throughout the whole phenological cycle, both, at the original location growing wild and at the experimental plot as cultivated plants. Local pedoclimatic conditions seem to be crucial for the plant essential oil production. Our results showed high conditioning rates from both yearly climatic conditions and developmental stage of the plants. Maximum yield production was reported at the full seed maturation stage (average 1.74%) and after a slight dry period (average 2.16%). Phytochemical differences were maintained when plants were forced to grow under common pedoclimatic conditions. Thereby, essential oil analysis showed some populations formed by clearly distinct individuals while others had more homogenous plants. Compounds such α-pinene, β-pinene + myrcene, limonene, 1,8-cineol, camphor and β-caryophyllene were the main compounds of the essential oils of S. lavandulifolia.


Sanchez-Vioque R.,Centro Agrario Of Albaladejito | Sanchez-Vioque R.,Fundacion Parque Cientif co y Tecnologico de Albacete | Izquierdo-Melero M.E.,Centro Agrario Of Albaladejito | Polissiou M.,Agricultural University of Athens | And 6 more authors.
Grasas y Aceites | Year: 2015

Solid residues from the hydrodistillation of selected Spanish populations of rosemary (Rosmarinus officinalis L.) have been analyzed for their polyphenol composition, and antioxidant and bioplaguicide activities. The objective was to evaluate and select the most suitable plant materials as sources of natural antioxidants and crop protectants. Total polyphenol content and polyphenol composition of rosemary populations were very dependent on the growth location: populations from Aranjuez showed a higher content of total polyphenols and were richer in rosmarinic acid as compared with their equivalent populations from Cuenca, whereas these latter were characterized by an overall higher content in genkwanin and carnosol. Most of the antioxidant activities were highly correlated with the total content of polyphenols although some polyphenols like carnosic acid and carnosol seemed to favor such activities. The extracts from R. officinalis were strong antifeedants against Leptinotarsa decemlineata Say and moderate against Spodoptera littoralis Boisd and Myzus persicae Sulzer, according to their feeding ecologies. The biological effects of the active samples cannot be accounted by their chemical composition, suggesting additive or synergistic effects. Both the phytotoxic and stimulating effects on Lactuca sativa L., and Lolium perenne L. leaf and/or root growth were observed.


Santana-Meridas O.,Centro Agrario Of Albaladejito | Santana-Meridas O.,Institute Recursos Humanos para la Ciencia y la Tecnologia INCRECYT | Polissiou M.,Agricultural University of Athens | Izquierdo-Melero M.E.,Centro Agrario Of Albaladejito | And 5 more authors.
Industrial Crops and Products | Year: 2014

Rosemary is one of the most important aromatic plants in terms of commercialization of essential oil. Nevertheless, a large amount of solid residue with a considerable content of bioactive compounds is generated during the hydrodistillation of the essential oil. In this work we have identified the major phenolic compounds present in such residue and evaluated its antioxidant and bioplaguicide activities. Antioxidant activity was determined by several in vitro methods, such as the β-carotene/linoleate model system, reducing power, DPPH radical scavenging, and iron and copper ion chelation. Bioplaguicide activity was investigated by means of the antifeedant effects against Leptinotarsa decemlineata Say, Spodoptera littoralis Boisd. and Myzus persicae Sulzer; and the phytotoxic activity against Lactuca sativa L., Lycopersicon esculentum L. and Lolium perenne L. Solid residue extract showed very high antioxidant activities, comparable to an extract from red grape pomace that was used as a natural antioxidant standard. It also showed strong antifeedant activity against L. decemlineata, S. littoralis and M. persicae with effective doses within the range of model antifeedants as well as limited phytotoxic effects on L. sativa, SL. esculentum and L. perenne. These results suggest a potential use of the solid residue from the hydrodistillation of rosemary as source of antioxidants and natural crop protectants. © 2014 Elsevier B.V.


Sanchez-Vioque R.,Centro Agrario Of Albaladejito | Sanchez-Vioque R.,Institute Recursos Humanos para la Ciencia y la Tecnologia INCRECYT | Polissiou M.,Agricultural University of Athens | Astraka K.,Agricultural University of Athens | And 5 more authors.
Industrial Crops and Products | Year: 2013

Essential oils of aromatic plants have numerous applications in fields like medicine, cosmetic and food industry. Nevertheless, the average yield of essential oil distilled from aromatic plants is below 5% (w/w) and consequently a considerable amount of solid residues is generated. These residues are especially rich in polyphenols that can be exploited as health-promoting compounds in food and feed or as anti-aging ingredients in cosmetic products, and thus to enhance the overall profitability of the aromatic plants. In this work, we have tentatively identified the polyphenols present in solid residues from the steam distillation of Cistus ladanifer, Lavandula×. intermedia, Santolina rosmarinifolia and Thymus mastichina, and evaluated their antioxidant and chelating activities by means of several in vitro methods, such as the β-carotene/linoleate model system, reducing power, DPPH radical scavenging, and iron and copper ion chelation. A Soxhlet extraction and an ultrasound-assisted extraction have been used in terms of comparison. Most of the extracts whether they were obtained by Soxhlet system or by ultrasonic method showed good antioxidant activities. Soxhlet ethanolic extract of T. mastichina showed an antioxidant activity comparable to the standard red grape pomace. The influence of the total polyphenol content on the chelating activities was not as important as on the antioxidant activities and the predominant role of certain polyphenols and/or the presence in the extracts of other compounds with a favourable chemical structure to metal complexation can be assumed. This was the case of the ultrasound ethanolic extract of C. ladanifer, which was the most effective in iron chelating activity despite its medium-to-low content of total polyphenols. All these data point to profit from these aromatic plant solid residues as a valuable source of bioactive compounds. © 2013 Elsevier B.V.


Sanchez-Vioque R.,Centro Agrario Of Albaladejito | Sanchez-Vioque R.,Institute Recursos Humanos para la Ciencia y la Tecnologia INCRECYT | Rodriguez-Conde M.F.,Centro Agrario Of Albaladejito | Reina-Urena J.V.,Centro Agrario Of Albaladejito | And 4 more authors.
Industrial Crops and Products | Year: 2012

Corms, tepals and leaves of saffron are by-products generated during the processing of the stigmas of the flower (saffron spice). To increase the overall profitability of this crop, these by-products have been investigated as a potential source of antioxidant compounds. With this purpose, several in vitro antioxidant methods, such as the β-carotene/linoleate model system, reducing power, DPPH and nitric oxide radical scavenging, and iron and copper ion chelation have been assayed on corm, tepal and leaf extracts. Best antioxidant properties were observed for leaf extract, which totally inhibited the oxidation of β-carotene at 10μg/mL and showed a DPPH scavenger activity up to 32 times higher than those reported for traditional sources of antioxidants like grapes and berries. Tepal extract showed an extensive inhibition of β-carotene oxidation, and significant scavenging NO radical and Cu 2+-chelating activities. In contrast, corm extract was a poor antioxidant although showed a slight Cu 2+-chelating activity. It can be concluded that tepals and especially leaves of saffron constitute an exploitable source of antioxidant and metal chelating compounds. © 2012 Elsevier B.V.


Laserna-Ruiz I.,Centro Agrario Of Albaladejito | de-Los-Mozos-Pascual M.,Centro Agrario Of Albaladejito | Santana-Meridas O.,Centro Agrario Of Albaladejito | Santana-Meridas O.,Institute Recursos Humanos para la Ciencia y la Tecnologia INCRECYT | And 3 more authors.
Euphytica | Year: 2012

Seed weevil (Bruchus spp.) is one of the most damaging pests to lentil crop, and the current chemical treatments to control this insect present numerous disadvantages. In this work, we have searched for resistant or almost resistant lentil cultivars to be used as potential sources of genes for resistance to bruchids as a more sustainable and environmentally friendly approach to diminish harvest loss. Thus, a lentil germplasm collection of 571 accessions from 27 different countries, and including wild species was screened for susceptibility to seed bruchids under natural field conditions in Central Spain. Infestation was evaluated in comparison with a check (lentil cultivar 'Lyda') according to the number of eggs per pod and the infestation rate in seeds (IR). A large variation of IR (0-70 %) was observed among accessions although, in general, wild species were consistently less affected by bruchids than the check. A total of 32 accessions, including Lens culinaris Medikus subsp. culinaris, Lens culinaris Medikus subsp. orientalis (Boiss.) Ponert, Lensnigricans (M. Bieb.) Godr., and Lenslamottei Cezfr., showed lower infestation rates than the check and were selected as potential sources of resistance to this pest. Potential mechanisms of resistance of selected accessions and their inclusion in a breeding program are outlined. © 2012 Springer Science+Business Media B.V.


Santana O.,CSIC - Institute of Agricultural Sciences | Santana O.,Centro Agrario Of Albaladejito | Reina M.,CSIC - Institute of Natural Products and Agrobiology | Fraga B.M.,CSIC - Institute of Natural Products and Agrobiology | And 2 more authors.
Chemistry and Biodiversity | Year: 2012

Crude extracts and fractions from Echium wildpretii H. Pearson ex Hook. f. subsp. wildpretii (Boraginaceae) have been tested against insect species Spodoptera littoralis, Leptinotarsa decemlineata, and the aphids Myzus persicae, Diuraphis noxia, Metopolophium dirhodum, Rhopalosiphum maidis, and Rhopalosiphum padi. The EtOH extract and the lipid and steroidal fractions of E. wildpretii exhibited significant antifeedant activities against the aphids and L. decemlineata. Two bioactive mixtures composed of fatty acid esters and n-alkanes were obtained from the lipid fraction. The bioguided fractionation of the steroidal fraction resulted in the isolation of glutinol, β-sitosterol, (3β,7α)-stigmast-5-ene-3,7-diol, and (3β,7α)-7- methoxystigmast-5-en-3-ol. The latter two compounds exhibited potent antifeedant activities against L. decemlineata indicating that the presence of an O-bearing C(7) was responsible for the activities of these molecules. Copyright © 2012 Verlag Helvetica Chimica Acta AG, Zürich.


PubMed | Centro Agrario Of Albaladejito
Type: Evaluation Studies | Journal: Analytical biochemistry | Year: 2011

A new colorimetric method based on the bleaching of the iodoplatinate ion has been developed for fast and easy determination of -glutamyl-S-ethenyl-cysteine (GEC) in narbon vetch (Vicia narbonensis L.) seeds. The calibration curve showed a good correlation (r(2)=0.9959) between absorbance and GEC amounts from 5.5 to 33 g (10-59.78 mol/L). The limits of detection and quantification were 1.16 and 3.55 mol/L, respectively, and no significant interferences from other sulfur-containing compounds were observed. The method showed excellent repeatability (relative standard deviation [RSD]=0.28%), reproducibility (RSD=4.4%), and accuracy (94%). Determination of GEC in 20 narbon vetch accessions yielded values that were in agreement with those reported previously using capillary electrophoresis and high-performance liquid chromatography methods. The method could be especially valuable for determination of GEC during the process of production of new low-GEC narbon vetch varieties.


Megias C.,CSIC - Instituto de la Grasa | Cortes-Giraldo I.,CSIC - Instituto de la Grasa | Alaiz M.,CSIC - Instituto de la Grasa | Giron-Calle J.,CSIC - Instituto de la Grasa | And 6 more authors.
Food Analytical Methods | Year: 2015

A method for determination of the neurotoxic non-protein amino acid 3-N-oxalyl-2,3-diaminopropionic acid (ODAP) and other free amino acids in Lathyrus cicera and Lahtyrus sativus is presented. Seed extracts were derivatised by reaction with diethyl ethoxymethylenemalonate (DEEMM) and analysed by reversed-phase high-performance liquid chromatography (RP-HPLC). Calibration curves showed very good linearity of the response. The limits of detection (LOD) and quantification (LOQ) were 0.15 and 0.50 μM, respectively. The method has a high intra- [relative standard deviation (RSD) < 0.42 %] and inter-repeatability (RSD = 2.01–2.33 %) and a remarkable accuracy with a 99 % recovery in spiked samples. The method yielded similar results in comparison with a previously established colorimetric method. The method is very easy to carry out and allows for ready analysis of large number of samples using very basic HPLC equipment because the derivatised samples are very stable and have very good chromatographic properties. © 2015, Springer Science+Business Media New York.

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