Central Veterinary Control and Research Institute

Ankara, Turkey

Central Veterinary Control and Research Institute

Ankara, Turkey

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Izgur M.,Ankara University | Akan M.,Ankara University | Ilhan Z.,Yuzuncu Yil University | Yazicioglu N.,Central Veterinary Control and Research Institute
Ankara Universitesi Veteriner Fakultesi Dergisi | Year: 2010

Caseous lymphadenitis (CLA), which is a serious infectious disease of sheep and goats world-wide, has caused significant economic losses in Turkey in recent years. In this study, it was aimed to identify major antigens of Corynebacterium pseudotuberculosis strains isolated in Turkey and to evaluate the efficiency of the vaccine prepared from local strains of C. pseudotuberculosis against bacterial challenge in lambs. C. pseudotuberculosis strains were isolated from six different flocks in Turkey and were classified according to their similarities by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE revealed more than 21 bands ranging in size from 15 kDa to 194 kDa present in different concentrations in C. pseudotuberculosis isolates as determined by visual assessment of their approximate molecular masses. Lambs were vaccinated with a bacterin + toxoid + Freund's complete adjuvant (FCA) vaccine, and the vaccine efficiency against bacteriological challenge was evaluated. When a local C. pseudotuberculosis (Pl 18) strain was used and formulated as per ml of the vaccine, 1.5x108 cfu of C. pseudotuberculosis bacterin + toxoid + 1/2 FCA of the toxoid, we obtained encouraging results regarding the potential of this vaccine to control of CLA in sheep.


Utuk A.E.,Central Veterinary Control and Research Institute | Kirbas A.,Atatürk University | Babur C.,Refik Saydam National Hygiene Center | Balkaya I.,Atatürk University
Israel Journal of Veterinary Medicine | Year: 2012

This study was conducted on 11 camels, ranging in age from 2 months to 40 years. The aim of this study was to detect the Toxoplasma gondii antibodies and helminth parasites in camels. T. gondii antibodies were detected by Sabin-Feldman Dye Test (SFDT), and faecal samples were examined by Fulleborn flotation and Benedek sedimentation methods. As a result of the study, T. gondii antibodies were detected in 10 out of 11 (90.9%) camels. Detected helminths consisted of 63.63% (7/11) Trishostrongyloidea spp., 45.45% (5/11) Trichuris spp. and 9.09% (1/11) Dicrocoelium dendriticum.


Cabuk T.Z.,Central Veterinary Control and Research Institute | Sari B.,Gazi University | Unal H.I.,Gazi University
Journal of Applied Polymer Science | Year: 2010

In this study, the conducting homopolymer of indene was synthesized by a chemical polymerization method in a nonaqueous medium, and polyindene (PIn)/polyoxymethylene (POM) blends were prepared. The physical, chemical, thermal, and spectral properties of the synthesized homopolymer and their blends were investigated. The conductivities of PIn and the PIn/POM blends were measured with a four-probe technique. The conductivity of PIn was determined as 1.16 × 10-5 S/cm, whereas the conductivities of the PIn/POM blends were determined to be in the range 3.16 × 10-6 to 9.8 × 10-6 S/cm. From Gouy scale magnetic susceptibility measurements, we found that PIn and the PIn/POM blends had polaron natures. The amount of Fe (milligrams per gram) in the PIn and PIn/POM structures were determined by inductively coupled plasma-optic emission spectrometry. Fourier transform infrared spectra were taken to analyze the structural properties of PIn and the PIn/POM blends. The thermal properties of PIn and PIn/POM blends were investigated with thermogravimetric analysis and differential scanning calorimetry analyses, and we found that they showed adequate thermal stability. According to the initial decomposition temperature among the blends, the blend including 16% PIn had the highest decomposition temperature with 244°C. The morphological structures of the PIn, POM, and blends were clarified with scanning electron microscopy. © 2010 Wiley Periodicals, Inc.


Utuk A.E.,Cukurova University | Piskin F.C.,Central Veterinary Control and Research Institute | Girisgin A.O.,Uludag University | Selcuk O.,Uludag University | Aydin L.,Uludag University
Apidologie | Year: 2016

In this study, we aimed to determine the prevalence of Nosema spp. in honeybees of Turkey. For this aim, adult honeybee (Apis mellifera) samples were collected from 1621 colonies within 95 apiaries located in 22 provinces of Turkey. Samples were examined microscopically. In case of positivity, spore identification was done by multiplex PCR. At the end of microscopic examination, Nosema spp. spores were detected in 7 out of 22 provinces (31.8 %), and 16 out of 95 colonies (16.8 %) that represent 1621 colonies. According to PCR results, 1 out of 16 isolates (6.25 %) was Nosema apis, and 15 out of 16 isolates (93.75 %) were Nosema ceranae. The result of our study indicated that N.ceranae is the dominant species in Turkey. © 2015, INRA, DIB and Springer-Verlag France.


Fidan A.F.,Afyon Kocatepe University | Cmgi C.C.,Afyon Kocatepe University | Karafakioglu Y.S.,Usak University | Utuk A.E.,Central Veterinary Control and Research Institute | And 2 more authors.
Journal of Animal and Veterinary Advances | Year: 2010

The aim of this study was to examine the effects of neosporosis on the oxidative stress cow naturally infected with Neospora caninum. Ten infected cow with Neospora caninum and ten healthy were included in this study. The neospora infection was diagnosed in the cows by using a commercially available competitive enzyme-linked immunosorbent assay kit Compared with healthy controls, levels of malondialdehyde and serum concentration of nitric oxide metabolites higher in the infected group. Furthermore, total antioxidant activities were found lowered in the infected group compared to the control group. The results demonstrated that oxidative stress markers are greatly increased in cows naturally infected with Neospora caninum. © Medwell Journals, 2010.


Simsek S.,Firat University | Utuk A.E.,Central Veterinary Control and Research Institute | Balkaya I.,Atatürk University
Helminthologia | Year: 2011

Summary: The most common and widespread liver flukes of the genus Fasciola are Fasciola hepatica and F. gigantica. Adults of both species occur in many domestic ruminants and in humans and can cause serious disease. The differential diagnosis of these flukes infection is very important because of their different transmission and epidemiological characteristics. A simple and rapid PCR-restriction fragment length polymorphism (RFLP) assay, using the common restriction enzymes AluI and RsaI, is described to distinguish between both fasciolid species. After the digestion of the mitochondrial cytochrome c oxidase 1 (CO1) PCR product with the restriction enzyme AluI, the RFLP profile obtained from F. hepatica revealed two fragments, whereas F. gigantica was not cut. The RsaI digestion generated two fragments from F. gigantica, whereas it did not cut the PCR product from F. hepatica. Results were confirmed with CO1 sequence analysis of both F. hepatica and F. gigantica. The present study suggests that the PCRRFLP method described here can be used for the proper identification of Fasciola species. © 2011 Versita Warsaw and Springer-Verlag Wien.


Balkaya I.,Atatürk University | Babur C.,Refk Saydam National Hygiene Center | Celebi B.,Refk Saydam National Hygiene Center | Utuk A.E.,Central Veterinary Control and Research Institute
Israel Journal of Veterinary Medicine | Year: 2011

Te aim of this study was to investigate the seroprevalence of Toxoplasma gondii in donkeys in Er-zurum, the largest province of Eastern Turkey. Te current study was carried out on serum samples collected from 55 female and 37 male donkeys from the Erzurum province which were examined using the Sabin Feldman Dye test. Out of 92 collected samples, 57 (62%) of sera were found to be se-ropositive at the dilution of 1:16 and greater. Te rates of positivity for female and male donkeys were 67.3% and 54.1%, respectively. As far as age groups were concerned, 38.6% positivity was found at 0-3 years, 50.9% at 4-6 years and 19.3% at 7 years of age or older. Tis study indicated that toxoplasmosis in donkeys is common in Erzurum province and displays diferent ratios in terms of age and gender.


Utuk A.E.,Central Veterinary Control and Research Institute | Simsek S.,Firat University | Piskin F.C.,Central Veterinary Control and Research Institute | Balkaya I.,Atatürk University
Israel Journal of Veterinary Medicine | Year: 2011

The aim of this study was to detect Neospora caninum IgG antibodies in Saanen and Hair goats from Elazig, Erzurum and Ki{dotless}rsehir provinces of Turkey. For this, a total of 128 sera obtained from goats were tested using a commercially available competitive enzyme-linked immunosorbent assay (c-ELISA) kit. Overall prevalence of anti-N.caninum antibodies was 10.2% (13/128); and mean prevalence in Hair and Saanen goats were 13.8% (12/87) and 2.4% (1/41) respectively. Tis is the frst serological study on caprine neosporosis in the above mentioned provinces of Turkey.


Bedir O.,Gulhane Military Medical Academy | Kilic A.,Gulhane Military Medical Academy | Atabek E.,Central Veterinary Control and Research Institute | Kuskucu A.M.,Gulhane Military Medical Academy | And 2 more authors.
Polish Journal of Microbiology | Year: 2010

Leptospirosis, caused by pathogenic Leptospira, is one of the most important zoonoses in the world. Several molecular techniques have been developed for detection and differentiation between pathogenic and saprophytic Leptospira spp. The aim of this study was to develop a rapid and simple assay for specific detection and differentiation of pathogenic Leptospira spp. by multiplex real-time PCR (TaqMan) assay using primers and probes targeting Leptospira genus specific 16S ribosomal RNA gene, the pathogen specific lig A/B genes and non-pathogen Leptospira biflexa specific 23S ribosomal RNA gene. Sixteen reference strains of Leptospira spp. including pathogenic and nonpathogenic and ten other negative control bacterial strains were used in the study. While the 16S primers amplified target from both pathogenic and non-pathogenic leptospires, the lig A/B and the 23S primers amplified target DNA from pathogenic and non-pathogenic leptospires, respectively. The multiplex real-time PCR (TaqMan) assay detection limit, that is, the sensitivity was found approximately 1 × 102 cells/ml for lig A/B gene and 23S ribosomal RNA gene, and 10 cells/ml 16S ribosomal RNA. The reaction efficiencies were 83-105% with decision coefficients of more than 0.99 in all multiplex assays. The multiplex real-time PCR (TaqMan) assay yielded negative results with the ten other control bacteria. In conclusion, the developed multiplex real-time PCR (TaqMan) assay is highly useful for early diagnosis and differentiation between pathogenic and non-pathogenic leptospires in a reaction tube as having high sensitivity and specificity.

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