Central Tasar Research and Training Institute

Ranchi, India

Central Tasar Research and Training Institute

Ranchi, India
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Yogananda Murthy V.N.,Institute of Management Sciences | Lokesh G.,Central Tasar Research and Training Institute | Munirajappa,Bangalore University | Dayakar Yadav B.R.,Central Sericultural Research and Training Institute
International Journal of Pharmaceutical Sciences Review and Research | Year: 2013

Ten mulberry varieties viz., Tr8, Tr12, Tr20, S1708, MS5, C6, C10, Matigara black, Morus nigra and M5 were analysed for their leaf quality through phytochemical tests. Results revealed that, total proteins, total sugars and amino acids were high in tender followed by medium and coarse leaves. Total proteins, total sugars and amino acids were high in S1708 leaves and total proteins and total sugars were low in M5 leaves and amino acids were low in C6 leaves. Phenols, prolines and chlorophyll contents were high in medium followed by coarse and tender leaves. Phenols were high in M5 and least in Matigara black leaves. Prolines were high in Matigara black and low in M5 leaves. S1708 leaves recorded highest total chlorophyll, chlorophyll-a and chlorophyll-b whereas C6 recorded lowest total chlorophyll, chlorophyll-a and Chlorophyll-b. Moisture contents were high in tender followed by medium and coarse leaves. Moisture content and moisture retention capacity were significantly high in S1708 and lowest in C6 leaves.


Kiran Kumar K.P.,Central Tasar Research and Training Institute | Sankar Naik S.,Andhra University
International Journal of Zoological Research | Year: 2011

In the silkworm rearing, the usage of commercial silkworm hybrids resistant/tolerant to silkworm diseases is economical and better option particularly in tropical countries, where inadequate disinfection methods are practiced. Among the major silkworm diseases, grasserie caused by Bombyx mori Nuclear Polyhedrosis Virus (BmNPV) is controlled by polygens. In the present study, an attempt was made to develop BmNPV tolerant polyvoltine x bivoltine hybrids by utilizing newly evolved BmNPV tolerant polyvoltine breeds viz., AKP 1, AKP 2 and AKP 3 as lines and bivoltine breeds viz., AKB 6, AKB 7 and AKB 8 as testers by employing LinexTester analysis. Nine hybrid combinations were compared with the control hybrids viz., Pure MysorexCSR 2 and APM lxAPS 8. Total score of positive traits of Specific Combing Ability (SCA), mid Parental Heterosis (MPH) and Better Parent Heterosis (BPH) was observed maximum in case of AKP l×AKB 6 with 20 fallowed by AKP l×AKB 8 (19), AKP 2×AKB 7 (13), AKP 3xAKB 6 (13). Least total score was observed in the case of AKP l×AKB 7. Based upon the total scores (SCA, MPH and BPH), over dominance and E.I values, AKP lxAKB 6 and AKP l×AKB 8 were identified as promising tolerant hybrids against BmNPV with productive merits. These tolerant hybrids can be suggested to the farmers for utilization after conducting large scale trials. © 2011 Academic Journals Inc.


Kumar D.,Central Tasar Research and Training Institute | Pandey J.P.,St Xaviers College | Jain J.,Central Tasar Research and Training Institute | Mishra P.K.,Central Tasar Research and Training Institute | Prasad B.C.,Central Tasar Research and Training Institute
International Journal of Zoological Research | Year: 2011

In the present study, quantitative and qualitative changes in protein profile of different tissue of larvae, pupae, adult and eggs of Tasar silkworm Antheraea mylitta Drury was investigated. Stage and age dependent variation in protein concentration and SDS-PAGE protein profile of 36 and 64 kDa protein was observed in different tissue. The concentration of protein was recorded higher in eggs laid by fresh moth than 3 days old moth and significant variation was also noticed in normal and depressed eggs. Interestingly, substantial changes in SDS-PAGE protein profile was observed in normal and depressed eggs and eggs laid by fresh moth than 3 days old moth. Haemolymph and midgut protein concentration was recorded higher in 3rd and 5th instar feeding larvae and in 4th instar mature larvae. Concentration of protein in the haemolymph of pupae before the brain window becomes opaque was higher in both the sexes than opaque stage. Fat body protein concentration in larvae showed increasing trend from 3rd to 5th instar larvae and it was higher in pupae after the brain window becomes opaque and fresh moth. In addition, higher protein concentration was recorded in gonads of pupae after the brain window becomes opaque and in reproductive organs of fresh moth. Present findings would promote to further understand the precise reason for depression of eggs and changes in protein profile in different tissue of A. mylitta. © 2011 Academic Journals Inc.


Jena K.,Central Tasar Research and Training Institute | Kumar Kar P.,Central Tasar Research and Training Institute | Kausar Z.,Central Tasar Research and Training Institute | Babu C.S.,Central Tasar Research and Training Institute
Journal of Thermal Biology | Year: 2013

High temperatures are known to cause physiological stress in organisms. This is often associated with enhanced generation of reactive oxygen species (ROS) leading to oxidative damage. The commercially important tropical tasar silkworm Antheraea mylitta has to endure high summer temperature before egg production on the onset of monsoon. In this study the status of pro-oxidants and antioxidants was studied in the testes of male pupae of tasar silkworm A. mylitta under thermal stress condition. Further, to find out the impact of temperature on physiological activity, oxygen consumption rate was measured. The result indicated higher level of thiobarbituric acid reactive substances (TBARS, as an index of lipid peroxidation) and total hydroperoxides in the male pupae exposed to high temperature (40±1°C). Similarly, it was found that increased levels of antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), ascorbic acid (ASA) and low molecular thiols (L-SH) in testes are more prominent in high temperature rather than in moderate temperature (35±1oC) suggesting the activation of physiological mechanism to scavenge the ROS produced during stress. Further more, the order of higher level of oxygen consumption rate was observed as high temperature (40±1°C) > moderate temperature (35±1°C) > control groups (28±1°C). Oxygen consumption rate was positively correlated with oxidative stress and antioxidant defence indices. We infer from these findings that the testes of A. mylitta pupae modulate testicular antioxidant responses to thermal stress. © 2013 Elsevier Ltd.


Singh G.P.,Central Tasar Research and Training Institute | Sinha A.K.,Central Tasar Research and Training Institute | Kumar P.K.,Central Tasar Research and Training Institute | Prasad B.C.,Central Tasar Research and Training Institute
Research Journal of Microbiology | Year: 2011

Tropical tasar silkworm, Antheraea mylitta D., an economically important insect is affected by bacteriosis caused by bacteria, which accounts considerable loss of 10-15% to silk cocoon production. The aim of the present investigation was to isolation, characterization and identification of bacteria causing diseases in Indian tropical tasar silkworm, Antheraea mylitta D. Total 15 isolates of bacteria in two groups (8 from anal lip sealing diseased silkworms and 7 from rectal protrusion diseased worms) were isolated. The shape, size and colour of bacterial colony were recorded. The gram reaction of vegetative cells, its shape, size and pattern of reaction with different enzymes were observed for characterization of different bacterial isolates. Pathogenecity of these bacteria have shown that only two bacterial isolates coded SA3 and RP2 were responsible for anal lip sealing and rectal protrusion diseases, respectively in tasar silkworm. The bacterial isolates coded SA3 and RP2 on the basis of cultural, morphological and biochemical characters tentatively identified as Serratia sp. which were, close to Serratia nematodiphila and Serratia marcescens sub sp., respectively. The infection of anal lip sealing and rectal protrusion diseases in Indian tropical tasar silkworm caused by Serratia nematodiphila and Serratia marcescens was reported first time. © 2011 Academic Journals Inc.


Prasad B.C.,Central Tasar Research and Training Institute | Pandey J.P.,Central Tasar Research and Training Institute | Sinha A.K.,Central Tasar Research and Training Institute
American Journal of Food Technology | Year: 2012

Cocoonase which is secreted as a natural phenomenon has its direct utility in softening of cocoons for reeling without altering the very organic nature of tasar silk. In the present study, efforts have been made to comprehend and utilize cocoonase for its future use in cocoon cooking. The emerging moth gradually release cocoonase from galea of their mouth parts in anterior inner portion of the cocoon (peduncle region). After releasing adequate volume of enzyme (around 400-600 μL) for softening the peduncle region of cocoon, with the help of appendages emerging moth create outlet near the peduncle and escape from cocoon shell. Around 1000 mL cocoonase from 2000 eclosion stage A. mylitta have been collected and centrifuged in cold condition (4°C) at 10000 rpm to minimise the impurity. To maintain buffer conditions, cocoonase was collected in pre chilled Tris buffer pH 9.2 and stored at -4°C temperature for further use in cocoon cooking. When cocoons of A. mylitta (Daba ecorace) were subjected to initial water boiling at 100°C on leisurely flame for 30-40 min followed by cocoon soaking in cocoonase for 20-24 h at 37°C, the 80-90% softening of cocoon shell was found. Silk filament obtained from the cocoons cooked in cocoonase maintains natural tasar silk colour, softness and structure. The 50-52% silk recovery was observer in cocoonase cocoon cooking. By centrifuging used reaction mixture at 9000 rmp in cold condition to remove impurities and adding 10-15% volume of fresh cocoonase in reaction mixture left over enzyme solution can be reused once. More study is required to get better cocoonase cooking efficiency and consistent cooking with higher or comparable silk recovery than the ruling practices. © 2012 Academic Journals Inc.


Reddy R.M.,Central Tasar Research and Training Institute
Asian Textile Journal | Year: 2010

The major host plants of all silkworms are available as cultivated or nature grown under different agro climatic conditions and their rearing has become prerogative for rural farmers in India. The silk proteins are extensively used as anti-hay fever masks, gauze pads, contact lenses, catheters and bandages, as artificial skin, blood vessels, tendons, ligaments to treat dermatological disorders besides as anti-coagulants in specialized surgeries. The eye catching art of cocoon craft is very resourceful utility of pierced silk cocoon and one of the cultural and traditional products in certain states of India. The silk bio-polymer is used in manufacturing eye lenses, tissue restoration of burn victims, and as matrix of abrasion surgery. The pupal oil has cosmetic value and is used extensively in face powders and creams, body deodorants besides as hair darkener and shaper with its capacity of making hair black, supple, and flexible.


Kiran Kumar K.P.,Central Tasar Research and Training Institute | Sinha A.K.,Central Tasar Research and Training Institute | Gupta S.K.,Central Tasar Research and Training Institute | Madhusudhan K.N.,Central Tasar Research and Training Institute | Prasad B.C.,Central Tasar Research and Training Institute
International Journal of Zoological Research | Year: 2011

Pebrine disease in tasar silkworm is considered to be most serious because of its chronic pathogenicity. Prophylactic method of mother moth examination proves to be the foolproof technique for pebrine identification. So, the study was conducted to investigate pebrine spores detection competency and evaluation of their quality in exhisting mother moth examinations. Four existing mother moth examination methods namely Prick and See, Conventional, Fuziwara and Delayed Mother Moth Examination (DMME) by Fuziwara were selected for the study. The maximum pebrine and hatching percentages were noticed in 4 days DMME with 23.2 and 93.1%, respectively. While, minimum values were observed in prick and see with 4.3% and 84.3, respectively. No significant variations in Cocoon weight, Shell weight and S.R% was observed in cocoons obtained from the different mother moth testing methods. Least percentage of effective rate of rearing (ERR) was observed in Prick and See method (58.75%) followed by Conventional method (61.25%) and Fuziwara (63.15%). High percentage of ERR was observed in DMME which ranged from 64.53% (1st day after egg laying) to 67.28% (4th day after egg laying). Least percentage of improvement of ERR% over Prick and See was observed in Conventional (2.50%) followed by Fuziwara (4.40%). High percentage of ERR over Prick and See was observed in DMME which ranged from 5.78% (1st day) to 8.53% (4th day). Consequently, among the tested mother moth examinations, DMME by Fuziwara after delaying four days from the egg laying has given best results. This method can be implemented in the research institutions for the maintenance of germplasm and breeders stock. © 2011 Academic Journals Inc.


Manohar Reddy R.,Central Tasar Research and Training Institute
Journal of Entomology | Year: 2010

The commercially important tropical tasar silk insect, Antheraea mylitta Drury (Lepidoptera: Saturniidae) is having 44 ecoraces distributed along central India (12-31°N latitude and 72-96°E longitude) with varied phenotypic, physiological and behavioral characters. The sericigenous insect feeds primarily on Shorea robusta (Sal), Terminalia arjuna (Arjun), Terminalia tomentosa (Asan) besides variety of secondary and tertiary food plants available in tropical deciduous forests of West Bengal, Jharkhand, Bihar, Orissa, Chhattisgarh, Madhya Pradesh, Uttar Pradesh Maharashtra and Andhra Pradesh states of India. Some wild tasar ecoraces like, Raily (Chhattisgarh), Modal and Jata (Orissa), Sarihan and Laria (Jharkhand), Bhandara (Maharashtra) and Andhra (Andhra Pradesh), besides domesticated Daba and Sukinda are contributing for livelihood and alleviating the socio-economic status of around hundred and fifty thousand Indian tribal families. However, the extensive collection of nature grown cocoons, rapid deforestation and human encroachment to insect habitats has declined ecorace population. So, there is an imperative need to involve and educate local tribals on cocoon collection and insect conservation to save from extinction. The orderly involvement of forest, tribal welfare, sericulture departments and Non Government Organizations (NGOs) along with local aboriginals can up-keep the silk insect conservation for sustainable utility. This coordinated management of tasar insect population will also help in rejuvenation of their semi-domesticated relative seri-biodiversity and integrated bio-resource (flora and fauna) management of insect ecozones. © 2010 Academic Journals Inc.


Manohar Reddy R.,Central Tasar Research and Training Institute | Sinha M.K.,Central Tasar Research and Training Institute | Prasad B.C.,Central Tasar Research and Training Institute
Journal of Entomology | Year: 2010

In spite of huge availability of nature grown tropical tasar silkworm food plants and rural tribal man power, the tasarculture and raw silk production is yet to attain the potential. The reason being the inadequacy of prospective commercial silkworm seed and breed options, and the urgent need is a coherent application of existing parental races by effective selection. The global demand for vanya silks in general and tasar silk in particular, call-for sustainable utilization of country's seri-biodiversity potential. Viability and productivity proportion of tasarculture in terms of seeds, cocoons and essentially raw silk, need attention for its vital role in reforming the livelihood and economic condition of rural, backward and tribal farmers. The conventional approaches on basic stock maintenance, commercial seed production, selective use of parental races or parents for heterosis and heterobeltiosis, method of backcrossing to exploit the traits of commercial importance and applying the advantage of GenotypexEnvironment (GxE) interactions are indispensable. In spite of current knowledge on sophisticated transgenic silkworm, appropriate application of on-hand parental resource material and methodologies can expedite tasar silk productivity improvement in addition to up-keep the agro based cottage industry's cost-effectiveness and biodiversity conservation. The review deals with the current situation and probable strategies for enhancing the productivity and quality of tasar raw silk. © 2010 Academic Journals Inc.

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