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Vinodh Kumar O.R.,Central Sheep and Wool Research Institute CSWRI | Gunaseelan L.,Madras Veterinary College | Sekar M.,Madras Veterinary College | Ronald B.S.M.,Madras Veterinary College | Sakthivelan S.M.,Madras Veterinary College
Buffalo Bulletin | Year: 2014

From two organized farms with previous histories of paratuberculosis, 93 female buffaloes aged between 2 and 5 years were selected for screening for paratuberculosis using a cattle type ELISA kit. The milk and serum ELISA were performed and the association between ELISA S/P ratios was correlated. The correlation found was highly significant (0.816**, P=0.00). From this study it is concluded that milk ELISA can be a better alternative than serum ELISA since collection of blood from buffaloes is a very tedious while collection of milk is very simple. © 2014, Kasetsart University. All rights reserved. Source

Kumar D.,Central Sheep and Wool Research Institute CSWRI | De K.,Central Sheep and Wool Research Institute CSWRI | Saxena V.K.,Central Sheep and Wool Research Institute CSWRI | Naqvi S.M.K.,Central Sheep and Wool Research Institute CSWRI
Journal of Animal and Feed Sciences | Year: 2015

A major problem in sheep rearing is scarcity of feed during the summer and winter in semi-arid tropics. A study was conducted to assess the sperm motion characteristics and sexual behaviour of Garole x Malpura x Malpura (GMM) rams subjected to nutritional stress. Eighteen adult GMM rams were randomly allocated into three groups of six animals each: G1 (control; fed maintenance requirement), G2 (fed 20% less than maintenance) and G3 (fed 30% less than maintenance). The animals were stall-fed with a diet consisting of 70% roughage and 30% concentrate. The study was conducted for eight weeks during the spring. Semen collection was done weekly at 08:00. Semen was evaluated by a computer-assisted sperm analysis technique. Sexual behaviours were also recorded at the time of semen collections. The proportion of rapid, medium and slow motile sperm, and average path velocity varied significantly (P < 0.05) among the groups. The time for seeking females significantly (P < 0.05) decreased in G3, while the refractory period significantly (P < 0.05) increased in G2. The testosterone concentration was significantly (P < 0.05) lower in G2 and G3 as compared with G1. The results indicate that GMM rams compromised their reproductive performance under nutritional stress imposed by 30% feed restriction of their maintenance diet during a feed scarcity period. © 2015, Polish Academy of Science. All rights reserved. Source

Kumar J.,Central Sheep and Wool Research Institute CSWRI | Tripathi B.N.,Central Sheep and Wool Research Institute CSWRI | Kumar R.,Animal Biotechnology Section | Sonawane G.G.,Central Sheep and Wool Research Institute CSWRI | Dixit S.K.,Central Sheep and Wool Research Institute CSWRI
Tropical Animal Health and Production | Year: 2013

Corynebacterium pseudotuberculosis, a Gram-positive bacterium is the causative agent of caseous lymphadenitis (CLA), a chronic disease of sheep, goats and other warm blooded animals. In the present study, a total of 1,080 sheep reared under semi-intensive system on organized farms situated in the semi arid tropical region of Rajasthan, India, was clinically examined. Pus samples from superficial lymph nodes of 25 (2.31 %) adult sheep showing clinical lesions similar to CLA were collected for laboratory analyses. On the basis of morphological, cultural and biochemical characteristics 12 (48 %) bacterial isolates from pus identified it as C. pseudotuberculosis. A polymerase chain reaction (PCR) assay targeting Putative oligopeptide/dipeptide ABC transporter, nicotinamide adenine dinucleotide phosphate (NADP) oxidoreductase coenzyme F420-dependent and proline iminopeptidase (PIP) genes of C. pseudotuberculosis was developed that showed 14 pus samples as positive. All C. pseudotuberculosis isolates were also found positive for these genes in the PCR. The specificity of the PCR products was confirmed by sequencing of the amplified products that showed 98-100 % homology with published sequences available in the NCBI database. The present study shows the incidence of CLA as 2.31 %, 1.1 % and 1.29 % based on clinical, bacterial culture and direct pus PCR assay, respectively. The PCR assay was rapid, specific and as significant as bacterial culture in detecting bacteria directly in the clinical pus samples. The PCR assay developed in the study can be applied for the diagnosis and control of CLA. Furthermore, the assay can also be applied to detect C. pseudotuberculosis in various clinical samples. © 2013 Springer Science+Business Media Dordrecht. Source

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