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Natukula K.,Bhagwan Mahavir Medical Research Center | Natukula K.,Nizams Institute of Medical Sciences | Jamil K.,Bhagwan Mahavir Medical Research Center | Pingali U.R.,Nizams Institute of Medical Sciences | And 2 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2013

Background: The XRCC1 (X-ray repair cross complimenting group-I) gene in BER (base excision repair) pathway is essential for DNA repair process. Polymorphisms in this gene are associated with variations in the repair efficiency which might predispose individuals to development of various cancers. Two variants of XRCC1gene (at codon 399), Gln/Gln and Arg/Gln, have been shown to be related to lowered DNA repair capacity and increased genomic instability in multiple studies. Hence our investigation focused on genotyping these variants to correlate with other multiple risk factors in lung cancer (NSCLC) patients since we hypothesized that these variants of the XRCC1 gene might influence disease susceptibility. Materials and Methods: We examined the frequency of the polymorphism in one hundred cases and an almost equal number of controls after recording their demographics with a structured questionnaire. Genomic DNA from blood samples was extracted for PCR studies, followed by RFLP to determine the variants. The significance of the data was statistically analyzed. Results: The three genotypes in cases and controls were Arg/Arg (40% and 54.45%); Gln/Gln (19% and 9.90%), and Arg/Gln (41.0% and 35.64%) respectively. Among these 3 genotypes, we found Gln/Gln and Arg/Gln to show association with lung cancer. Correlating these genotypes with several parameters, we also found that these two variants were associated with risk in males (p<0.05) and with smoking habits (p<0.05). In females Arg/Gln genotype showed association with stage of the disease (p=0.04). This is the first report in South Indian scenario where Arg399Gln genotypes were found to be associated with stage of the disease in females. Conclusions: It is concluded that XRCC1 genotypes Gln/Gln and Arg/Gln may influence cancer susceptibility in patients with smoking habits and these functional SNPs in XRCC1 gene may act as attractive candidate biomarkers in lung cancer for diagnosis and prognosis.

Penchala Pratap G.,Sri Venkateswara University | Sudarsanam G.,Sri Venkateswara University | Husain K.,Central Research Institute of Unani Medicine
International Journal of Pharma and Bio Sciences | Year: 2014

Effect of ethanol, methanol and petroleum ether extracts from the leaves of Cardiospermum canescens Wall. was studied against human pathogenic bacterial strains of Escherichia coli, Salmonella typhi and Klebsiella pneumonia and the antibiotic sensitivity of bacterial strains was also analyzed by using Bauer-Kirby method. Finally leaf powder was subjected to physicochemical and preliminary phytochemical evaluations to establish the quality and purity of the drug. In the Physicochemical studies, loss on drying, total ash value, acid insoluble ash, water insoluble ash, various extractive values etc., were carried out. Further, qualitative tests for various functional groups like triterpenoids, alkaloids, glycosides etc., were carried out. HPTLC profile was also established with ethyl acetate, and methanolic extracts. The results obtained from the study concluded that leaf powder of this plant is a great potential source as an antibacterial agent and preliminary phytochemical studies revealed the presence of triterpenoids, alkaloids, tannins, flavonoids and proteins.

Natukula K.,Bhagwan Mahavir Medical Research Center | Natukula K.,Nizams Institute of Medical Sciences | Jamil K.,Bhagwan Mahavir Medical Research Center | Pingali U.R.,Nizams Institute of Medical Sciences | And 2 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2013

Background: The wide spectrum of clinical features in advanced stages of non-small cell lung cancer (NSCLC) probably contributes to disparities in outcomes because of different prognostic variables significant for stage IIIB/IV patients. Hence the aim of this study was to check for favorable response of patients to various chemotherapeutic combinations with respect to patient survival in stage IIIB and stage IV NSCLC disease. We selected those patients for our study who were receiving treatment with paclitaxel, gemcitabine or etoposide in combination with platinum based drugs. Materials and Methods: Seventy-two patients who visited the hospital from June 2009 to November 2012 with confirmed diagnosis of lung cancer were included, and data were collected for follow up and classified according to treatment received with respect to patients' regimen and response, and overall survival. This study analyzed tumor variables that were associated with clinical outcome in advanced NSCLC patients who were undergoing first-line chemotherapy for stage IIIB/IV NSCLC. Results: Comparative data on various parameters like age, gender, stage, histology, site of disease, metastatic site and chemo-regimens was analyzed; these parameters predicted variable significant improvement for overall survival (p=0.05). One and two year survival rates were 20.8% and 15.3% . Conclusions: In this study we found slight improvement in survival rates in NSCLC and clinical outcomes with one combination (carboplatin+paclitaxel). Overall there were only marginal differences in survival rates for other chemo-regimens evaluated in this study.

Rasheed N.M.A.,Indian Institute of Chemical Technology | Nagaiah K.,Indian Institute of Chemical Technology | Waheed M.A.,Central Research Institute of Unani Medicine
International Journal of Phytomedicine | Year: 2012

More than 70% of India's 1.1 billion populations still use non-allopathic systems of medicine like Ayurveda, Unani, Homeopathy and other systems. The global knowledge about Unani and Indian herbals will hopefully be enhanced by information on the evidence-base of these medicinal plants. Resin of Shorea robusta Gaertn known as "Raal" in Unani; Shala in Ayurvedic System belonging to Dipterocarpaceae family plays a foremost role in the Unani system of medicine. It is abundantly available in different parts of Eastern Ghats of Southern Peninsular India and has been widely used in indigenous system for the preparation of different formulations used in the treatment of many skin diseases mainly allergies; diarrhea, dysentery, astringent and as an ingredient in ointments. Due to its therapeutic potentials and vital medicinal properties and also usage of drug in most of the common ailments, lead us to standardize the drug according to WHO guidelines. Phytochemical and physico-chemical studies, macroscopic and microscopic properties of resin were carried out. The main aspects included in the study are organoleptic characters, physico-chemical constants, fluorescence analysis of powdered drug and extracts, TLC profile and Heavy metal analysis, which provide information, which are widely accepted in the quality assessment of herbal drugs and to lay down the standard for the genuine drug. Phytochemical screening, isolation of chemical constituents will help in future to study the pharmacological properties.

Ramakrishnan P.,Center for Development of Spirituality as Medical Subject | Ramakrishnan P.,Harvard University | Karimah A.,Airlangga University | Kuntaman K.,Airlangga University | And 8 more authors.
Journal of Religion and Health | Year: 2014

Religious/spiritual (r/s) characteristics of physicians influence their attitude toward integrative medicine and spiritual care. Indonesia physicians collaborate with traditional, complementary, and alternative medicine (TCAM) professionals within modern healthcare system, while Indian physicians are not reported to do so. The aim of the study was to understand the r/s characteristics and their influence on Indian and Indonesian physicians’ acceptance of TCAM/spirituality in modern healthcare system. An exploratory, pilot, cross-cultural, cross-sectional study, using Religion and Spirituality in Medicine, and Physician Perspectives (RSMPP) survey questionnaire, compared r/s characteristics and perspectives on integrative medicine of 169 physicians from two allopathic, Sweekar-Osmania University (Sweekar-OU), India, University of Airlanga (UNAIR), Indonesia, and a TCAM/Central Research Institute of Unani Medicine (CRIUM) institute from India. More physicians from UNAIR and CRIUM (89.1 %) described themselves as “very”/“moderately” religious, compared to 63.5 % Sweekar-OU (p = 0.0000). Greater number of (84.6 %) UNAIR physicians described themselves as “very” spiritual and also significantly high (p < 0.05) in intrinsic religiosity as compared to Sweekar-OU and TCAM physicians; 38.6 % of UNAIR and 32.6 % of CRIUM participants reported life-changing spiritual experiences in clinical settings as against 19.7 % of Sweekar-OU; 92.3 % of UNAIR, compared to CRIUM (78.3 %) and Sweekar-OU (62 %), felt comfortable attending to patients’ spiritual needs, (p = 0.0001). Clinical comfort and not r/s characteristics of participants was the significant (p = 0.05) variable in full regression models, predictive of primary outcome criteria; “TCAM or r/s healing as complementary to allopathic treatment.” In conclusion, mainstreaming TCAM into healthcare system may be an initial step toward both integrative medicine and also improving r/s care interventions by allopathic physicians. © 2014, Springer Science+Business Media New York.

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