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Hou H.,CAS Wuhan Institute of Hydrobiology | Hou H.,Agriculture and Agri Food Canada | Hou H.,Central Research Institute of Food and Feed Control | Atlihan N.,Agriculture and Agri Food Canada | And 2 more authors.
Frontiers in Plant Science | Year: 2014

Cisgenesis is genetic modification to transfer beneficial alleles from crossable species into a recipient plant. The donor genes transferred by cisgenesis are the same as those used in traditional breeding. It can avoid linkage drag, enhance the use of existing gene alleles.This approach combines traditional breeding techniques with modern biotechnology and dramatically speeds up the breeding process.This allows plant genomes to be modified while remaining plants within the gene pool. Therefore, cisgenic plants should not be assessed as transgenics for environmental impacts. © 2014 Hou, Atlihan and Lu.

Yibar A.,Uludag University | Ozcan A.,Central Research Institute of Food and Feed Control | Karaca M.Y.,Central Research Institute of Food and Feed Control
Kafkas Universitesi Veteriner Fakultesi Dergisi | Year: 2014

Detection of macrolides residues in animal feedingstuffs to ensure food safety is very important issue. For this purpose, a liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of residues of 4 macrolides (erythromycin, spiramycin, tilmicosin and tylosin) in animal feedingstuffs. Roxithromycin (ROX), a macrolide not used in veterinary medicine, was used as internal standard. The mass spectral acquisition was done in the positive-ion mode applying multiple reaction monitoring with the following ions (mass-to-charge ratio, m/z): m/z 837.4→679.2; m/z 734.4→576.2; m/z 422.4→174.1; m/z 869.1→696.1 and m/z 916.4→772.4 for roxithromycin (ROX), erythromycin (ERY), spiramycin (SPI), tilmicosin (TIL), and tylosin (TYL), respectively. Good repeatibility, reproducibility and recovery values was obtained. Average recoveries ranged from 98.9% (TIL) to 101% (ERY) and an overall mean of 99.9%. Method limits of detection (LODs) of ERY, SPI, TIL and TYL 5.1, 6.6, 6.7 and 7.5 μg kg-1 were achieved respectively. Method limits of quantification (LOQs) were 8.5, 11.1, 11.2 and 12.4 μg kg-1 for the same drugs, respectively. Satisfactory decision limits (CCα) and detection capabilities (CCβ) were also attained. The method is simple, rapid, sensitive and suitable for the simultaneous determination of macrolide antibiotics in animal feedingstuffs.

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