Central Research Institute of Field Crops

Ankara, Turkey

Central Research Institute of Field Crops

Ankara, Turkey

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Akfirat F.S.,Gebze Institute of Technology | Aydin Y.,Marmara University | Ertugrul F.,Scientific and Technological Research Council of Turkey | Hasancebi S.,Scientific and Technological Research Council of Turkey | And 5 more authors.
Cereal Research Communications | Year: 2010

Bulk segregant analysis (BSA) was used to identify molecular markers associated with yellow rust disease resistance in wheat ( Triticum aestivum L.). DNAs isolated from the selected yellow rust tolerant and susceptible F 2 individuals derived from a cross between yellow rust resistant and susceptible wheat genotypes were used to established a "tolerant" and a "susceptible" DNA pool. The BSA was then performed on these DNA pools using 230 markers that were previously mapped onto the individual wheat chromosomes. One of the SSR markers (Xgwm382) located on chromosome group 2 (A, B, D genomes) was present in the resistant parent and the resistant bulk but not in the susceptible parent and the susceptible bulk, suggesting that this marker is linked to a yellow rust resistance gene. The presence of Xgwm382 was also tested in 108 additional wheat genotypes differing in yellow rust resistance. This analysis showed that 81% of the wheat genotypes known to be yellow rust resistant had the Xgwm382 marker, further suggesting that the presence of this marker correlates with yellow rust resistance in diverse wheat germplasm. Therefore, Xgwm382 could be useful for marker assisted selection of yellow rust resistances genotypes in wheat breeding programs. © 2010 Akadémiai Kiadó.


Senturk Akfirat F.,Gebze Institute of Technology | Ertugrul F.,Scientific and Technological Research Council of Turkey | Hasancebi S.,Scientific and Technological Research Council of Turkey | Aydin Y.,Marmara University | And 4 more authors.
Journal of Genetics | Year: 2013

We have previously reported Xgwm382 as a diagnostic marker for disease resistance against yellow rust in Izgi2001 × ES14 F2 population. Among the same earlier tested 230 primers, one SSR marker (Xgwm311) also amplified a fragment which is present in the resistant parent and in the resistant bulks, but absent in the susceptible parent and in the susceptible bulks. To understand the chromosome group location of these diagnostic markers, Xgwm382 and Xgwm311, in the same population, we selected 16 SSR markers mapped only in one genome of chromosome group 2 around 1-21 cM distance to these diagnostic markers based on the SSR consensus map of wheat. Out of 16 SSRs, Xwmc658 identified resistant F2 individuals as a diagnostic marker for yellow rust disease and provided the location of Xgwm382 and Xgwm311 on chromosome 2AL in our plant material. © 2013 Indian Academy of Sciences.

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