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Mateen A.,Central Research Institute for Unani Medicine | Tanveer Z.,Shadan Institute Of Pg Studies | Janardhan K.,P.A. College | Gupta V.C.,Central Research Institute for Unani Medicine
International Journal of Pharma and Bio Sciences | Year: 2015

Antibiotics have been effective in treating infectious diseases, but resistance to these drugs has led to the emergence of new and the reemergence of old infectious diseases. For centuries, Indian spices have made a significant contribution both in the health care system and the food industry. Ancient Asian literature is a treasure of information related to the problems of health care and other environmental aspects. Bacterial strains used for the study was S. aureus (ATCC 25923), E. coli (ATCC 25922), P. aeruginosa (ATCC 27853) and P. vulgaris (ATCC 6380) were purchased from Hi-Media laboratories. Our results showed that various buffer pH have different protein extractability percentages after dialysis for the seed extracts used. The protein was extracted by sodium phosphate citrate buffer pH (7.2) highest concentration of the protein was found to ( 660μg/ml) in Ammi majus and the lowest was 160 μg/ml in . The highest protein concentration extracted by CTAB buffer pH (6.0) was found to be (640μg/ml) in Ammi majus and the lowest was 140 μg/ml in Chichorium intybus.

Syed R.,Bhagawan Manahir Hospital and Research Center | Syed R.,Central Research Institute for Unani Medicine | Prasad G.,Bhagawan Manahir Hospital and Research Center | Deeba F.,Bhagawan Manahir Hospital and Research Center | And 5 more authors.
African Journal of Microbiology Research | Year: 2011

Nosocomial infections are one of the occupational biohazards that affect the health of individuals with or without predisposing factors. Staphylococcus aureus is associated with significantly higher mortality and is associated with community-acquired serious nosocomial infections because strains generally show multiple drug resistance, which limits treatment possibilities. A total of 1800 patients in the state of Andhra Pradesh were screened for the presence of Staphylococcus species and were tested for antibiotic resistance. The results indicated that among ten antibiotics used in the present study, Amikacin and Azithromycin should be the drug of choice to treat S. aureus infection. It was observed that the resistance of most of the antibiotics tested showed increased resistance with increasing age. These results suggest that clinicians should consider age as an important factor while prescribing these antibiotics. © 2011 Academic Journals.

Al Akeel R.,King Saud University | Al-Sheikh Y.,King Saud University | Mateen A.,Central Research Institute for Unani Medicine | Syed R.,King Saud University | And 2 more authors.
Saudi Journal of Biological Sciences | Year: 2014

A huge group of natural antimicrobial compounds are active against a large spectrum of bacterial strains causing infectious threat. The present study was conducted to investigate the crude extracts of antimicrobial protein and peptide efficacy from six medicinal plant seeds. Extraction was carried out in Sodium phosphate citrate buffer, and Sodium acetate buffer using different pH. Antimicrobial activities of these plants were determined by the microbiological technique using Agar well diffusion Assay. Extremely strong activity was observed in the seed extracts of Allium ascolinicum extracted in sodium phosphate citrate buffer at pH (5.8) against Proteus vulgaris, Escherichia coli and Staphylococcus aureus with zone of inhibition 17. mm, 17. mm and 15. mm and Rumex vesicarius at pH (7.6), Ammi majus at pH (6.8), Cichorium intybus at pH (7.4) and Cucumis sativus at pH (7.8) also showed better sensitivity against the bacterial strains with zone of inhibition ranges 16-10. mm and some of the strains were found to be resistant. Antibacterial activity pattern of different plant extracts prepared in sodium acetate buffer pH (6.5), among all the plant seed extracts used Foeniculum vulgare had shown good inhibition in all the bacterial strains used, with zone of inhibition ranges 11-12.5. mm, The extracts of C. intybus and C. sativus were found to be effective with zone of inhibition 11-6. mm and some of the strains were found to be resistant. Most of the strains found to have shown better sensitivity compared with the standard antibiotic Chloramphenicol (25. mcg). Our results showed that the plants used for our study are the richest source for antimicrobial proteins and peptides and they may be used for industrial extraction and isolation of antimicrobial compounds which may find a place in medicine industry as constituents of antibiotics. © 2013.

Al Akeel R.,King Saud University | Mateen A.,Central Research Institute for Unani Medicine | Gupta V.C.,Central Research Institute for Unani Medicine | Janardhan K.,P.A. College
Journal of Environmental Biology | Year: 2016

Antimicrobial proteins/peptides produced by plant seeds participate in protection of seeds against pathogenic organisms. A study was carried out to investigate the in silico analysis of protein sequence localization, structure, homology modeling and 3D structure prediction of ALLCE-AMP in Allium cepa. Primary structure prediction and physico-chemical characterization were performed by computing theoretical isoelectric point (pI), molecular weight, total number of positive and negative residues, extinction coefficient, instability index, aliphatic index and grand average hydropathy (GRAVY). In the present study, homology modeling, a high quality of peptide 3D structure, was predicted by submitting the peptide sequence (target) to ESYPred3D web server. The template (1T12 chain A) was found to share 18.2% identity with the Query (B2CZN8). The model was validated using protein structure checking tools PROCHECK and ERRAT VALUE (62.353). The present study would be useful in studying protein-protein interactions and drug designing. © Triveni Enterprises, Lucknow (India).

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