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Ionescu D.,University of Bucharest | Chifiriuc M.C.,University of Bucharest | Ionescu B.,University of Bucharest | Ionescu B.,Central Reference Laboratory Synevo | And 9 more authors.
Romanian Biotechnological Letters

Streptococcus pyogenes is a Gram-positive bacterial pathogen responsible for a wide variety of human diseases. The aim of this study was to investigate the rate of Str. pyogenes infections and to determine the virulence patterns in isolates obtained from patients diagnosed in the SYNEVO Laboratory in Bucharest, during December 2012 - April 2013. Material and methods: The strains were isolated from throat swabs, identification was performed by conventional tests and by using a Bruker Maldi-Tof analyzer. The bacterial adherence to the cellular substratum was assessed by using the adapted Cravioto's method. The expression of soluble virulence factors was assessed by inoculating the Str. pyogenes strains on enriched culture media. Results: From the total of the 10691 individuals, 560 (5.14%), were found to be Str. pyogenes carriers. All tested strains adhered to the HeLa cells exhibiting different adherence patterns. At least one of the investigated soluble virulence factors with DN-ase being the most prevalent (77.1%). was produced by the streptococcal studied strains. Conclusions: The Str. pyogenes strains isolated from throat carriage in Romanian population exhibited a high adherence capacity to epitelial cells and expressed at least one of the investigated soluble virulence factors, suggesting that Str. pyogenes possess a high variety of pathogenic features that can be correlated with the high frequency, diverse location and severity of the produced infections. © 2015 university of bucharest. Source

Cristea V.-C.,Central Reference Laboratory Synevo | Cristea V.-C.,Carol Davila University of Medicine and Pharmacy | Gheorghe I.,University of Bucharest | Neacsu G.,Central Reference Laboratory Synevo | And 6 more authors.
Romanian Biotechnological Letters

The aim of this study was to investigate the contribution of Staphylococcus species to the etiology of community acquired urinary tract infections (UTI) in Romanian population, and to investigate their antibiotic resistance profiles. 6,250 urine samples were collected between August - October 2014 by Synevo Laboratory (Bucharest), out of which the overall isolation rate of Staphylococcus spp. was 2.68% (n = 50). S. saprophyticus was the most frequent isolated species, being susceptible to methicillin but 28% exhibited an efflux-mediated macrolides resistance phenotype. S. aureus was the second most commonly isolated, exhibiting combined MRSA (methicillin-resistant Staphylococcus aureus) (encoded by SSCmec type III and IVa gene cassettes) and inducible resistance to macrolide, lincosamide, streptogramin B (MLSBi) phenotypes. The S. haemolyticus and S. lugdunensis strains were isolated with a lower frequency (2 strains each) and one S. lugdunensis strain harboured SSCmec type III cassette. Our results highlight differences in the etiology of community acquired UTI from those reported in other geographical areas, proving the necessity of knowing the local etiology and resistance markers in order to design appropriate empirical therapy regimens and to monitor the antimicrobial resistance emergence and spread in community. © 2016 University of Bucharest. Source

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