Time filter

Source Type

Luthra S.K.,ICAR Central Potato Research Institute Campus | Tiwari J.K.,ICAR Central Potato Research Institute | Lal M.,ICAR Central Potato Research Institute Campus | Chandel P.,ICAR Central Potato Research Institute | Kumar V.,ICAR Central Potato Research Station
Potato Research | Year: 2017

Ten tetraploid interspecific potato somatic hybrids developed earlier through protoplast fusion between the dihaploid Solanum tuberosum L. ‘C-13’ and the diploid wild species Solanum pinnatisectum Dun. were used in this study. Somatic hybrids and standard control cultivars were evaluated for adaptability, tuber traits, late blight resistance and keeping quality attributes (dormancy, sprouting, weight loss and appearance after 75 days of storage) during two successive winter crop seasons (short-days) in the sub-tropical plains of India, where nearly 90% of India’s potatoes are grown. Somatic hybrids showed medium to good plant vigour and late to very late plant maturity. Though none of the somatic hybrids produced equal or higher tuber yield than the control cultivars, except clone P7, all other somatic hybrids produced significantly higher marketable and total tuber yield after a 90-day growth than the parent C-13. Most of the somatic hybrids possessed higher tuber dry matter concentration, resistance to late blight and better keeping quality attributes than the control cultivars. Notably, the tubers of somatic hybrids showed a tendency for colour change from white to purple on exposure to sunlight. Breeding potential of somatic hybrids was further assessed by crossing them with commonly grown local potato cultivars for the development of backcross (BC1) progenies. In the hybridization programme, berries were formed while somatic hybrids were used either as male or female parents with common cultivars. Our results revealed that the use of bulk pollen of somatic hybrids not only resulted in higher pollination success but also helped to achieve higher numbers of true potato seeds (TPS) per berry. Despite the formation of berries, TPS was absent in 22 crosses using somatic hybrids as a female or male parent which may be due to ovule or embryo abortions. Segregating BC1 generations were evaluated under the same sub-tropical conditions for further exploitation in potato breeding. Based on our study, the somatic hybrids P4, P8 and P10 can be utilized for the introgression of important characters such as high tuber dry matter concontration, resistance to late blight and excellent keeping quality attributes into the cultivated potato via conventional breeding methods for cultivar development in the sub-tropical plains of India. © 2017 European Association for Potato Research


Jeevalatha A.,ICAR Central Potato Research Institute | Siddappa S.,ICAR Central Potato Research Institute | Kumar A.,ICAR Central Potato Research Institute | Kaundal P.,ICAR Central Potato Research Institute | And 4 more authors.
Virus Research | Year: 2017

Apical leaf curl disease, caused by tomato leaf curl New Delhi virus-[potato] (ToLCNDV-[potato]) is one of the most important viral diseases of potato in India. Genetic resistance source for ToLCNDV in potato is not identified so far. However, the cultivar Kufri Bahar is known to show lowest seed degeneration even under high vector levels. Hence, microarray analysis was performed to identify differentially regulated genes during ToLCNDV-[potato] infection in a resistant (Kufri Bahar) and a susceptible cultivar (Kufri Pukhraj). Under artificial inoculation conditions, in Kufri Pukhraj, symptom expressions started at 15 days after inoculation (DAI) and then progressed to severe symptoms, whereas no or only very mild symptoms were observed in Kufri Bahar up to 35 DAI. Correspondingly, qPCR assay indicated a high viral load in Kufri Pukhraj and a very low viral load in Kufri Bahar. Microarray analysis showed that a total of 1111 genes and 2588 genes were differentially regulated (|log2 (Fold Change)| > 2) in Kufri Bahar and Kufri Pukhraj, respectively, following ToLCNDV-[potato] infection. Gene ontology and mapman analyses revealed that these altered transcripts were involved in various biological & metabolic processes. Several genes with unknown functions were 5 to 100 fold expressed after virus infection and further experiments are necessary to ascertain their role in disease resistance or susceptibility. This study gives an insight into differentially regulated genes in response to ToLCNDV-[potato] infection in resistant and susceptible cultivars and could serve as the basis for the development of new strategies for disease management. © 2017 Elsevier B.V.


Sanju S.,ICAR Central Potato Research Institute | Siddappa S.,ICAR Central Potato Research Institute | Thakur A.,ICAR Central Potato Research Institute | Shukla P.K.,Sam Higginbottom Institute of Agriculture | And 4 more authors.
Functional and Integrative Genomics | Year: 2015

RNA interference (RNAi) has proved a powerful genetic tool for silencing genes in plants. Host-induced gene silencing of pathogen genes has provided a gene knockout strategy for a wide range of biotechnological applications. The RXLR effector Avr3a gene is largely responsible for virulence of oomycete plant pathogen Phytophthora infestans. In this study, we attempted to silence the Avr3a gene of P. infestans through RNAi technology. The P. infestans inoculation resulted in lower disease progression and a reduction in pathogen load, as demonstrated by disease scoring and quantification of pathogen biomass in terms of Pi08 repetitive elements, respectively. Transgenic plants induced moderate silencing of Avr3a, and the presence and/or expression of small interfering RNAs, as determined through Northern hybridization, indicated siRNA targeted against Avr3a conferred moderate resistance to P. infestans. The single effector gene did not provide complete resistance against P. infestans. Although the Avr3a effector gene could confer moderate resistance, for complete resistance, the cumulative effect of effector genes in addition to Avr3a needs to be considered. In this study, we demonstrated that host-induced RNAi is an effective strategy for functional genomics in oomycetes. © 2015, Springer-Verlag Berlin Heidelberg.


PubMed | Sam Higginbottom Institute of Agriculture, Banasthali University, ICAR Central Potato Research Institute and Indian Agricultural Research Institute
Type: Journal Article | Journal: Functional & integrative genomics | Year: 2015

RNA interference (RNAi) has proved a powerful genetic tool for silencing genes in plants. Host-induced gene silencing of pathogen genes has provided a gene knockout strategy for a wide range of biotechnological applications. The RXLR effector Avr3a gene is largely responsible for virulence of oomycete plant pathogen Phytophthora infestans. In this study, we attempted to silence the Avr3a gene of P. infestans through RNAi technology. The P. infestans inoculation resulted in lower disease progression and a reduction in pathogen load, as demonstrated by disease scoring and quantification of pathogen biomass in terms of Pi08 repetitive elements, respectively. Transgenic plants induced moderate silencing of Avr3a, and the presence and/or expression of small interfering RNAs, as determined through Northern hybridization, indicated siRNA targeted against Avr3a conferred moderate resistance to P. infestans. The single effector gene did not provide complete resistance against P. infestans. Although the Avr3a effector gene could confer moderate resistance, for complete resistance, the cumulative effect of effector genes in addition to Avr3a needs to be considered. In this study, we demonstrated that host-induced RNAi is an effective strategy for functional genomics in oomycetes.


Buckseth T.,ICAR Central Potato Research Institute | Saggoo Singh M.I.,Punjabi University
Cytologia | Year: 2016

Meiotic behaviour of plant chromosomes is influenced by both genetic and environmental factors. The present paper shows the effect of abnormal meiosis on pollen fertility. Meiotic restitution is considered to be a common mechanism of polyploidization in plants and hence is one of the most important processes in plant speciation. Pollen mother cells (PMCs) of cultivated potato from anthers at different stages of meiotic process were analyzed for their chromosomal behaviour and irregularities. Various meiotic irregularities; univalent and multivalent formation, chromosome bridges, lagging chromosomes, etc. were reported for the first time in India. © 2016 The Japan Mendel Society.


Sharma A.K.,ICAR Central Potato Research Station | Kumar V.,ICAR Central Potato Research Station | Pandey K.K.,ICAR Central Potato Research Institute
Potato Journal | Year: 2016

The conventional system of seed potato production involving clonal multiplications is being replaced with micro-propagation techniques (hi-tech system) on account of numerous advantages possessed by the later. High hills of northwestern India are ideally suited for the production of breeder seed potatoes. Thus, nucleus and breeder seed potatoes for the hilly regions of India are produced at Central Potato Research Station, Kufri (Fagu), Shimla hills at an average altitude of 2700m above mean sea level. Since 2008, seed potato production through micro-propagation techniques has also been introduced at the station. To know the comparative efficiency of both the systems of seed potato production, a study was conducted during 2011 and 2012 involving the important cultivars of the region. The study revealed that both the systems of seed potato production proved equally good with regard to plant growth vigour, disease freedom as well as for the tuber number of tubers and productivity. It can be attributed to the slow rate of degeneration of seed stocks in either of the production systems due to low vector pressure prevailing in high hills of north-western India. Among the cultivars, Kufri Himalini recorded the maximum yield with minimum number of tubers per ha while Kufri Girdhari had the maximum number of tubers with mini-yields. © 2016, Indian Potato Association. All rights reserved.


Mehta A.,ICAR Central Potato Research Station | Singh B.,ICAR Central Potato Research Station | Singh B.,ICAR Central Potato Research Institute
Indian Journal of Horticulture | Year: 2016

Heap storage of potatoes is commonly used in many states of India to avoid distress sale at harvest, but the losses in stored potatoes are generally enormous. Spray application of CIPC (isopropyl N-(3-chlorophenyl) carbamate) at the time of storage has been recommended to inhibit sprouting and reduce total losses in potatoes up to 90 days of storage in heaps. Sprouting in tubers was inhibited and total losses in potatoes were reduced (by 58.7%) up to 90 days of storage (temp. 19–31°C, 55–90% RH) during March to June. The farmers could market 6.5% more weight of CIPC treated potatoes (cv. Kufri Pukhraj) compared to the control (untreated) tubers due to reduced total losses and fetch 55.3% higher market price than the price at the time of harvest. In processing cultivar, Kufri Chipsona-1, reducing sugar concentrations decreased from 188.1 to 22.5 mg/100 g fresh weight during storage up to 90 days and chip colour improved significantly. Stored potatoes were found highly acceptable for processing by an industry collaborator (M/s Satnam Agri Products Ltd., Jalandhar) and were used in making good quality flakes and French fries. Findings established that the improved storage technology can beneficially be used to increase remunerations from potato cultivation and to preserve the quality of processing potatoes for three months at lower storage cost. © 2016 Horticulture Society of India. All Rights reserved.


Mehta A.,ICAR Central Potato Research Station | Singh B.,ICAR Central Potato Research Institute
Potato Journal | Year: 2015

Considerable quantities of potato (Solanum tuberosum L.) are transported to different consumer centers through out the country and also exported to neighboring countries. Sprouting of potatoes is the major post-harvest limiting factor. Sprout inhibition treatment with isopropyl 3-chlorophenyl carbamate (CIPC) was evaluated under ambient conditions (20 to 35°C, 44 to 86% RH) in five potato varieties viz. Kufri Chipsona-1, Kufri Surya, Kufri Jyoti, Kufri Pukhraj and Kufri Bahar after harvest and after storage at 2-4°C for four months. CIPC treatment (15 and 20 mg a.i./kg tuber weight) significantly reduced sprouting and total losses in freshly harvested potatoes of all the varieties up to 60 days. Whereas in cold stored potatoes, CIPC treatment was effective at higher concentrations (25 and 30 mg a.i./kg tuber weight) in only two varieties. Nevertheless, the treatments could usefully extend the shelf life of potatoes by 30 to 45 days. CIPC treatment reduced sugar contents and improved the chip colour. Freshly harvested potatoes of Kufri Chipsona-1 can be marketed as table as well as processing potatoes, while other cultivars were suitable for table purpose only. Cold stored potatoes can be marketed only as table potatoes. CIPC residues were below the permissible limits and potatoes were safe for human consumption. The information is of high practical utility to market/export potatoes to distant markets. © 2015, Indian Potato Association. All rights reserved.


Singh S.K.,ICAR Central Potato Research Station | Lal S.S.,ICAR Central Potato Research Institute
Potato Journal | Year: 2015

The area under autumn/early potato crop in Bihar is around 15%. The productivity of early potato crop is very low due to high temperature prevailing during growth and development period of the crop. Kufri Surya, a heat tolerant variety of potato developed by Central Potato Research Institute, Shimla, performs better than other varieties when temperatures are higher during planting and early growth period. The field trials were conducted in randomized block design during autum/ winter season of 2011-12 and 2012-13 at farmer’s fields in participatory mode with the objective to assess the suitability of potato variety Kufri Surya for early planting under warmer condition in the Sone riverbed of Patna district in Bihar. The potato variety Kufri Surya recorded higher emergence percentage, significantly taller plants, more number of leaves per plant, higher LAI as compared, to potato cultivar Lal Gulab. It also produced highest total tuber yield, aggregate tuber population, average tuber weight, nutrient uptake, gross return and net return with recommended package of practices. Kufri Surya also produced 26.4% and 5.7% higher total tuber yield and total tuber number, respectively as compared to potato variety Lal Gulab at farmer’s management level. It also recorded 50.5 and 63.2% higher marketable tuber yield at farmer’s management and improved package of practice, respectively. © 2015, Indian Potato Association. All rights reserved.


Apical leaf curl disease, caused by Tomato leaf curl New Delhi virus-[potato] (ToLCNDV-[potato]) is one of the most important viral diseases of potato in India. Genetic resistance source for ToLCNDV in potato is not identified so far. However, the cultivar Kufri Bahar is known to show lowest seed degeneration even under high vector levels. Hence, microarray analysis was performed to identify differentially regulated genes during ToLCNDV-[potato] infection in a resistant (Kufri Bahar) and a susceptible cultivar (Kufri Pukhraj). Under artificial inoculation conditions, in Kufri Pukhraj, symptom expressions started at 15days after inoculation (DAI) and then progressed to severe symptoms, whereas no or only very mild symptoms were observed in Kufri Bahar up to 35 DAI. Correspondingly, qPCR assay indicated a high viral load in Kufri Pukhraj and a very low viral load in Kufri Bahar. Microarray analysis showed that a total of 1111 genes and 2588 genes were differentially regulated (|log2 (Fold Change)|>2) in Kufri Bahar and Kufri Pukhraj, respectively, following ToLCNDV-[potato] infection. Gene ontology and mapman analyses revealed that these altered transcripts were involved in various biological & metabolic processes. Several genes with unknown functions were 5 to 100 fold expressed after virus infection and further experiments are necessary to ascertain their role in disease resistance or susceptibility. This study gives an insight into differentially regulated genes in response to ToLCNDV-[potato] infection in resistant and susceptible cultivars and could serve as the basis for the development of new strategies for disease management.

Loading ICAR Central Potato Research Institute collaborators
Loading ICAR Central Potato Research Institute collaborators