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Shin S.,Seoul National University | Lee H.,Central Post Entry Quarantine Station | Lee S.,Seoul National University
Journal of Asia-Pacific Entomology

Since 2007, serious collapses of the bedlogs used to cultivate shiitake mushrooms in Korea have been reported. An investigation of these shiitake mushroom farms revealed two Camptomyia spp. (C. corticalis and C. heterobia) as major pests. These gall midges feed on mycelium of the shiitake mushroom, thereby disturbing the formation of mushroom fruit bodies. Because they are not known pests of shiitake mushrooms, we provide taxonomic and biological descriptions of these two Camptomyia species. This report represents new records of C. corticalis (Loew) and C. heterobia Mamaev in Far East Asia. © 2011 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Source

Shinohara A.,National Museum of Nature and Science | Hara H.,Forestry Research Institute Doto Station | Lee H.-S.,Central Post Entry Quarantine Station | Lyu D.,Sangji University
Proceedings of the Entomological Society of Washington

Descriptions are given for the adults of both sexes and immature stages of Arge hasegawae Takeuchi, 1927, based on the holotype and newly acquired material. Rubus crataegifolius Bunge (Rosaceae) is newly recorded as a host plant. The life history is recorded for the first time based on rearing of material collected in Suwon, Gyeonggi-do, Korea. Arge hasegawae is a multivoltine species with gregarious larval habit at least in early instars. A previous record from Japan is a misidentification. Copyright © 2011 BioOne All rights reserved. Source

Park D.-S.,Korea Research Institute of Bioscience and Biotechnology | Suh S.-J.,Youngnam Regional Office | Hebert P.D.N.,University of Guelph | Oh H.-W.,Korea Research Institute of Bioscience and Biotechnology | Hong K.-J.,Central Post Entry Quarantine Station
Bulletin of Entomological Research

Although DNA barcode coverage has grown rapidly for many insect orders, there are some groups, such as scale insects, where sequence recovery has been difficult. However, using a recently developed primer set, we recovered barcode records from 373 specimens, providing coverage for 75 species from 31 genera in two families. Overall success was >90% for mealybugs and >80% for armored scale species. The G•C content was very low in most species, averaging just 16.3%. Sequence divergences (K2P) between congeneric species averaged 10.7%, while intra-specific divergences averaged 0.97%. However, the latter value was inflated by high intra-specific divergence in nine taxa, cases that may indicate species overlooked by current taxonomic treatments. Our study establishes the feasibility of developing a comprehensive barcode library for scale insects and indicates that its construction will both create an effective system for identifying scale insects and reveal taxonomic situations worthy of deeper analysis. © 2010 Cambridge University Press. Source

Shin S.-G.,Seoul National University | Lee H.-S.,Central Post Entry Quarantine Station | Lee S.,Seoul National University
Journal of Asia-Pacific Entomology

Seven species of sciarid flies were collected in shiitake mushroom farm in Korea. Among them, Lycoriella ingenua (Dufour 1839) and Bradysia difformis Frey 1948 were dominant as possible pests of the shiitake mushroom because the larvae were found on both oak bed logs and in the artificial sawdust beds for shiitake cultivation. Five other species, which were collected in lower numbers, are reported for the first time in Korea: Bradysia longimentula (Sasakawa 1994), Bradysia trispinifera Mohrig & Krivosheina 1979, Leptosciarella (Leptospina) subdentata (Mohrig and Menzel 1992), Scatopsciara camptospina Mohrig and Mamaev 1990, and Xylosciara inornata Mohrig and Krivosheina 1979. © 2011 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. Source

Park D.-S.,Korea Research Institute of Bioscience and Biotechnology | Jin Leem Y.,Hannam University | Hahn K.-W.,Hannam University | Suh S.-J.,Central Post Entry Quarantine Station | And 2 more authors.
Journal of Economic Entomology

Mealybugs are under a strict regulation at foreign trades of agricultural products because they are one of the most economically damaging groups of insects on food crops and ornamental plants. However, the absence of morphological characteristics enabling the discrimination of early life stages often cause a significant delay or rejection of a shipment when infested fruit is discovered, causing significant economic loss. A polymerase chain reaction-based method for species identification was developed for six mealybug species known to infest Korean pears including two regulated insects, Planococcus kraunhiae (Kuwana) and Crisicoccus matsumotoi (Siraiwa). Six sets of species-specific primers were designed based on the sequence comparison of the internal transcribed spacer 1 and 2 regions. Efficiency tests against 29 mealybug samples showed that this method could effectively discriminate different mealybug species regardless of their developmental stages. Blind tests against 11 field collected mealybug nymph samples indicated that a single polymerase chain reaction is enough to discriminate unidentified mealybugs collected on Korean pears. This new method will facilitate trade and export requirements, as well as identify the species at any stage of mealybug intercepted. © 2010 Entomological Society of America. Source

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